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دراسة وبائية لمرض اللشمانيات في محافظة النجف الاشرف == Epidemiological study of Lieshmaniasis in Najaf

Author name: يحيى بستان كاظم الموسوي
Supervisor name: محسن عبد نعمة الروضان
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Higher Diploma
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: Arabic
University location: Qadisiyah
First pages:

الكشف عن خمج الفايروس التاجي في الاطفال والعجول باستخدام تقنية الوقت الحقيقي لتفاعل سلسلة البلمرة في محافظة الديوانية == Detection of coronavirus infection in children and calves by using Real Time qRT - PCR in AL - Diwaniyia province

Author name: ختام قايد مايع
Supervisor name: محسن عبد نعمة الروضان
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:

التوصيف والجزيئي عن فايروس التاجي في الجمال والانسان == Seroepidemiological and Molecular Characterization of Coronavirus in Camel and Human

Author name: صبا فلاح كليف البو صالح
Supervisor name: محسن عبد نعمة الروضان
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:

تاثير العوامل الوبائية على انتشار السل البقري == The effect of epidemiological factors on bovine tuberculosis distributions

Author name: احمد علي حسن غالي البديري
Supervisor name: قاسم حليم كشاش
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Higher Diploma
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:

الاهمية الانتقالية والاقتصادية لداء المقوسات Toxoplasmosis في الانسان والحيوان == Transitional and economical important of Toxoplasmosis In human and animals

Author name: فاضل صعيب خليف
Supervisor name: منير عبد الامير الفتلاوي
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Higher Diploma
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: Arabic
University location: Qadisiyah
First pages:

الدراسة الجزيئية والسيرولوجية على العزلة المحلية لفايروس النيوكاسل المرضي في الفرات الاوسط من العراق == Molecular and serological study on local NDV isolate in Middle Euphrates of Iraq

Author name: احمد مهدي عباس كاظم
Supervisor name: هدى عبد الهادي علي النصراوي | احمد مجيد حمزة الشمري
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:

دراسة حول الاصابة بفيروس الروتا في الانسان والحيوان == Study about rotavirus infection in human and animals

Author name: علي فاهم سفاح الهلالي
Supervisor name: هدى عبد الهادي علي
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Higher Diploma
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:

عزل وتشخيص جزيئي لمورثات مجاميع الانماط المصلية لجرثومة اللستيريا المستوحدة Listeria monocytogenes من الانسان والحيوانات في محافظة القادسية == Isolation And Molecular Detection of Serotype Genes Group of Listeria Monocytogenes From Human And Animals In Al - Qadissyia Province

Author name: حسين عمران كريم العابدي
Supervisor name: هدى عبد الهادي علي النصراوي
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: اجريت الدراسة الحالية خلال الفترة من تشرين الثاني 2014 الى نيسان 2015، جمعت العينات المرضية من النساء التي تعاني من حالات الاجهاض وولادة مبكرة والاطفال المصابين بالتهاب السحايا من مستشفى الولادة والاطفال التعليمي وقد جمعت العينات تحت اشراف طبي متخصص وكان | This study was carried out during the period from November 2014 to April 2015, whereas samples were collected from women that suffered of abortion and infected children in the maternal and children hospital in Al - Qadissiya province , so the samples were collected under supervising of a specialist medicine , the number of taken samples of the women is 65 samples , while the number of taken samples of children is 32 samples , so the samples that belong to animals have included a gall bladder samples were collected from Al - Diwaniya slaughterhouse were 100 samples of sheep and 100 samples of cows. in addition to collect 200 samples of milk as 100 samples for each sheep and cows from different rural zones in al - Qadissiya province. L.monocytogenes was isolated by using oxford listeria selective agar for listeria , so the special stimulator for growth of the bacteria was added to it in order to determine the type of bacteria , also it diagnosed an isolates for L.monocytogenes by using the microscopically examination and biochemical tests , furthermore the germ isolates was planted on blood agar for diagnosing the blood analysis type : ? - heamolysis as affirmative checking for the isolates. also PCR technique used for affirming the diagnosing of L. monocytogenes by using the initiator for the blood analysis gene (hly) , whereas all the L. monocytogenes isolates' gave a positive result in inspection , so this technique is used too in the detection about the serous types for L.monocytogenes isolates by the molecular diagnosing for the fierce genes of the germ and these are (D1,D2,Glt,FlaA and MAMA gene ). The results of the research showed that isolating and diagnosing percentage of the Listeria monocytogenes is about 9.27%, whereas the isolation percentage in women infected in abortion is 4.61%, while the percentage of listeria germ isolation was higher in the children that infected in meningitis whereas it was 18.75% of the total amount of the samples.As for the percentage of the isolating in sheep it was 4% and 7% for milk and gall bladder respectively. In the cows the isolation percentage of the germ from milk and gall bladder was 2% and 3% respectively. So the the results of isolating for listeria germ during the study period in human and animals were disparate , whereas the highest percentage of isolation for the germ in human were scored in January and February months of the year whereas it scored 20% and 16% respectively.As for sheep the percentage of isolation was higher in December and January months whereas it is 6.25% and 10% respectively , while in the cows the percentage was higher in January and February months where it is 4.2% and 7.4% respectively. Regarding The results of detection about the genes of the serous types for the isolates of L.monocytogenes by using PCR technique 14 isolates of the samples showed a positive result when they react with the initiator for gene (D1)in percentage about 56%, so this isolates showed a positive result when they react with the initiator for gene (Glt) as amount of 5 isolates and they classified to be belong to the serous types (1/2B or 3B), while 9 isolates gave a negative result in the inspection , whereas these isolates were tested with the initiator for gene (MAMA), 5 isolates showed a positive result in this reaction with this gene and they classified to be belong to the serous types (4c or 4a), while 4 isolates gave a negative result as they classified to be belong to the serous types (4b or 4d or 4c).As for the isolates' that gave a negative result with the initiator of gene (D1)there are 11 isolates all these isolates gave a positive result when they inspected with the initiator of gene (D2) in a percentage about 44%, so when these isolates inspected with the initiator for gene (FLaA) then 6 isolates of them gave a positive result in the inspection and classified as they belong to (1/2a or 3a), while 5 isolates of them gave a negative result in the inspection and classified as they belong to (1/2c or 3c). As for the serous types that have not diagnosed in the germ isolates of the human there are(1/2a or 3c), while in the germ isolates of the sheep the serous types (4a or 4c) have not been diagnosed , then the serous types (1/2b or 3b) have not been scored in the cows. The present study concluded that the percentage of infection in L.monocytogenes was high in human in al - Qadissiya province , in which that refer to the risks of the diseases and health problems spread that could be caused by L.monocytogenes , also the results included isolating the germ from milk and gall bladder samples for sheep and cows that could be a source to carry the infection to the human by contaminating the meat, milk and its derivatives'. whereas the isolation percentage of the germ was higher in the cold months of the year in human and animals, so the highest isolation percentage of the serous types for the L.monocytogenes in human and cows were (4a , 4c) , while in sheep the serous types were (1/3c,3c).

التحري عن الاصابة بفيروس جدري الابل في بعض محافظات العراق باستخدام تقنية تفاعل سلسة انزيم البلمرة PCR

Author name: محمود شاكر عبد الامير عيدان مرزوق
Supervisor name: هدى عبد الهادي علي النصراوي
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: Arabic
University location: Qadisiyah
First pages:

تحديد القرابة الوراثية لعزلات عراقية ضارية لفيروس مرض النيوكاســــل من تتابع القواعد النتروجينية في موروث الهموكلوتنين نيورامنيديز == Phylogenetic Determination of Newcastle Disease Virus Hn Gene Sequences Iraqi Virulent Isolate

Author name: مرتضى عبد المهدي محمد حسن المظفر
Supervisor name: هدى عبد الهادي علي النصراوي | احمد مجيد حمزة الشمري
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:
Abstract: يعد مرض النيوكاسل من اخطر الامراض الفيروسية المعدية التي تصيب قطعان الدواجن وتسبب خسائر اقتصادية كبيرة في صناعة الدواجن واول تسجيل لمرض النيوكاسل كمرض مشترك بين الانسان والحيوان كان من قبل العالمBurnet في سنة 1943. والهدف من الدراسة هو عزل وتحليل تسل | Newcastle disease is considered to be the most contagious poultry disease and may cause severe economic loss in the poultry industry. The first report in which Newcastle disease virus (NDV) was consider as zoonotic disease and a human pathogen that was published by Burnet, in 1943. The aim of this study was to isolate and determination of nucleotide sequence of the Hemagglutinin - neuraminidase gene (HN) of two NDV Iraqi virulent isolates. These isolates were named as Najaf isolate (Najaf APMV1/ Chicken/ Iraq - Najaf/ ICCMGR/2012) and Baghdad isolate (Baghdad ICCMGR). This work was conducted in Iraq for the first time, the reason behind it was to correlate phylogenetically between these two isolates and the NDV strains in the countries of the region. This will help in determining the source of NDV outbreaks occurred in Iraq. Both Najaf and Baghdad isolates were propagated in embryonated chicken eggs, after inoculation of 11 days embryonated chicken eggs was showed and embryos were killed in different times (more than 40 hours and less than 72 hours). To assess the activity of both virus isolates, the main features of the infected embryos that reflect characteristics of congestion and sever hemorrhage was compared with control one. Hemorrhage was markedly severed in the infected embryos and it was similar in its severity by both isolates. The allantoic fluid was harvested post infection and the agglutination activity reflected titer of 1024 for Najaf isolate and 512 for Baghdad isolate. In order to isolate the Hemagglutinin - neuraminidase gene (HN) from both Najaf and Baghdad NDV isolates, specific primers for this gene were designed. After viral RNA purification, one - step reverse transcriptase - PCR was undertaken to amplify the NH gene and isolated it from the gel. Nucleotide sequence of the isolated NH gene from both virus isolates was determined. The purpose was to focus on molecular and phylogenetic analysis of the hemagglutinin - neuraminidase (HN) gene in both Iraqi isolates. The resulted nucleotide sequence of 647 nucleotides of the HN gene for both isolate were submitted to the GenBank database under the accession numbers KJ632972. To determine the origin of both Iraqi virulent isolates, HN gene sequence of both isolates was aligned with sequences of NDV isolates previously published in GenBank. The resulted alignment was then analyzed in terms of phylogenetic relation and variation. phylogenetic analysis of the isolated virus showed that both Iraqi isolates share significant similarity with 14 other international stains. Two strains of India origin and 12 was China isolates. The identity percentage was 99% - 100% with clustered group. Interestingly, Iraqi isolate was different from the neighbor countries such as Iran, and other Middle East countries. These results may indicate that certain migratory birds might have contributed to the distribution of NDV in Iraq. However imported infected beards from south East Asia may contribute to the current NDV outbreaks caused by foreign strain.

دراسة التغيرات الكيموحيوية في مصل الاغنام المصابة بداء الاكياس العدرية في الكبد == A study of Biochemical changes in serum of sheep infected with hydatidosis in liver

Author name: صبا فلاح كليف الصالحي
Supervisor name: غيداء عباس جاسم الجبوري
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: Arabic
University location: Qadisiyah
First pages:

دراسة وبائية عن داء الجيارديا في الانسان والابقار == An Epidemiological Study About Gairdiasis In Humans And Cows

Author name: شيماء عبد جبر الزيادي
Supervisor name: نعمان ناجي عايز
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Higher Diploma
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:

تشخيصCampylobacter jejuni باستخدام تقنية الوقت الحقيقي لتفاعل سلسلة البلمرة في محافظة المثنى == Detection Of Campylobacter Jejuni By Real - Time PCR In Al - Muthanna Province

Author name: منى توفيق عبد
Supervisor name: هدى عبد الهادي علي النصراوي
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Master
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: English
University location: Qadisiyah
First pages:

داء البروسيلات Brucellosis وافاق استخدام التقنيات الحديثة في تشخيصه == BRUCELLOSIS AND SOME RECENT TECHNIQUS THAT USED IN ITS DIAGNOSIS

Author name: احمد حسين علوان الغرابي
Supervisor name: احمد جاسم نعمة الهنداوي
General topic: Veterinary Medicine
Specific topic: Common Diseases
Degree: Higher Diploma
University: University of Al-Qadisiyah - College Of Veterinary Medicine
Language: Arabic
University location: Qadisiyah
First pages: