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التشخيص الجزيئي لبعض انواع الفطر Trichoderma وتقييم فعاليتها ضد الديدان الثعبانية لعقد الجذور Meloidogyne javanica في محافظة واسط == Molecular Diagnosis of Some Species of Trichoderma And Evaluation Their Activity Against Root - Knot Nematode Meliodogyne Javanica In Wasit Province

Author name: حمزة عباس ياسر
Supervisor name: محمد جبير حناوي | هادي مهدي عبود
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Wasit University - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Wasit
First pages:
Abstract: نفذت هذه الدراسة لعزل وتشخيص الانواع المستوطنة للفطرspp Trichoderma في محافظة واسط وتقييم فاعليتها ضد نيماتود العقد الجذرية Meloidogyne javanica (Treub) في مختبر الفطريات في قسم علوم الحياة - كلية العلوم - جامعة واسط فضلا عن دراسة تاثير هذه الانواع في بعض | This study has been conducted to isolate and identify some nation species of the fungus Trichoderma spp in wasit province and evaluate the activity of them against Root Knot nematode Meloidogyne javanica (Treub) in the laboratory of Biodepartment - College of science - University of wasit, and study the effect of these species in growth criteria of tomato plant in greenhouses of Ministry of Technology and Science, and diagnosed by PCR technology. Soil samples had been collected from soil and plant roots (solanaceae, cucurbitacece) infected with Meloidogyne javanica in fields and greenhouses in eight locations in wasit province (Kut center, Alahrar, Alhay, Alnuamania, , Badra, algardhia, sheikh saad and Aldijaily) for the period from 5/11/2013 to 22/12/2013.The results had been revealed that there are 15 isolates of 25 isolates contain Trichoderma spp, by the purification we got 7 a local isolates of the fungus Trichoderma belong to four species of Trichoderma which were T.harzianum، T.hamatum، Trichoderma sp1 and Trichoderma sp2.Results showed All tested isolate high efficiency in parasitism the eggs of nematode and the reduction of the percentage of hatching eggs and the more effective isolate was the T. harzianum3 (T6) from sheikh saad location comparing with the other tested isolates and the percent of parasitism was 97%, and significant differences with all isolates and the results showed a clear reduction in the number of larvae emerging comparing with the control.The results showed that the isolates T.hamatum1 (T1), T.harzianum2 (T3)، T.hamatum2(T7) exist high percent of parasitism which were 92.2% , 90.8% , 90.4% respectively It was not a significant difference between them, while the isolates T.harzianum1 (T2)، Trichoderma sp1 (T4)، Trichoderma sp2 (T5) were 80.4%, 77.5%, 84.6% respectively.The result also showed that the filtrate of isolates (100%, 50%, 25%) had good effect on egg hatching (destruction) comparing with the control and the more effective concentration was 100% Compared to other concentrations and the filtrate of isolate T. harzianum3(T6) was more effective comparing with other filtrate of isolates and the mortality of eggs were 96.7%، 75.5، % 56.3% at the concentration 100%, 50%, 25% respectively The Trichoderma sp2 (T5) was least effective isolate compared with other isolates, as the proportion of parasitism was 76.4%، 59.7%، 38.6% at the concentration 100%, 50%, 25% respectively. With regard to the effect of fungus in tomato plant growth criteria the results also showed that the isolate T. harzianum3 (T6) enhanced the growth of the length of stem and root of tomato plant growing in greenhouses and the lengths of stem and root treatment with T. harzianum3 (T6) were (19.7, 21.3) cm respectively Followed by the the control which were (16.1، 15.4) cm respectively while the lengths of stem and root of treatment plants with the fungus and nematode together were(14.4، 11.1) cm respectively and least length of stem and root of treatment plants with nematode only were (9.6، 6.2) cm respectively.The results of the polymerase chain reaction (PCR) revealed a Positive results by using SCAR primer in this technique to two isolate of fungus Trichoderma (T. harzianum and T. hamatum) through success in the amplification process to 7 isolate of the fungus as given expected band (837 base pairs) which back to the species T.harzianum, while the species T.hamatum the size of band was (450 base pairs), the recording of two species using this technique is the first in Wasit province and Iraq.