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تحديد الكفاءة التثبيطية لمضادي الاوكسي تتراسالين والتايلوسين المحضرين نانويا ضد البكتريا المسببه للالتهاب الضرع في الابقار في محافظة كربلاء

Author name: ميساء صالح مهدي الحمداني
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kerbala - College Of Science - Department Of Biology
University location: Karbala

انتاج وتوصيف متعدد السكريات الخارج خلوي Lactobacillus plantarum من بكتريا Exopolysaccharide المعزولة محليا ودراسة فعاليته البيولوجية == Production And Characterization of Exopolysaccharide (EPS) From Locally Isolated Lactobacillus Plantarum And Studying Its Biological Activity

Author name: بيداء مهدي عباس الغانمي
Supervisor name: ناجح هاشم كاظم الظويهري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kerbala - Faculty Of Education - Department Of Biology
Language: Arabic
University location: Karbala
First pages:
Abstract: Twenty - five isolates of Bacillus spp. Were isolated from 15 soil samples collected from different places in the Holly city of Kerbala, 13 isolates lactobacillus spp. Were isolated from 15 milk products and 7 isolates pseudomonas spp. The isolates were screened for their ability to produce Exopolysaccharide.The results showed that isolate number 3 (Lactobacillus lac 3) was found to give the highest production of the polymer, the isolate has been identified as lactobacillus plantarum. The optimal cultural and environmental conditions for EPS production were been identified which include using the production medium (simplified synthetic medium) containing date juice (5%) as a carbon source and yeast extract (0.2%) as a nitrogen source, the media was enhanced with a total concentration 0.9% of magnesium sulphate and potassium phosphate as mineral salts. The primary pH was adjusted to 6.5 and the inoculum size was 3%(v/v), isolates were grown under anaerobic condition at 35 Co for 24 h and the EPS extracted with ethanol 95%. Two steps were used for partial purification of the polymer as follows, the first step by using TriChloroAcitic acid (TCA) solvent and the second step by dialysis. The yields were (77.4 and 63.8)% for the two purification steps respectively. The biological activity of the EPS extraction from L. plantarum has been tested against some species of G+ve and G - ve bacteria and some fungal. The results showed that EPS extrac exhibits an inhibitory activity against Stenotrophomonas, Bacillus cereus and Proteus bacteria with an inhibition zone diameter of 23, 21, 19 mm respectively. While, there was no inhibitory effect against fungi. Antioxidant activity has also been tested, where IC50 value against ABTS free radical at 100 Mg / ml. The characterization of partially purified EPS is investigated by using Fourier Transform Infrared Spectroscopy (F.T.I.R). The molecular weight of the polymer was also measured by using viscometer and the molecular weight obtained by this technique was 2.3 *105 Dalton. The EPS extraction from selected isolate showed two spots when separated by Thin Layer Chromatography (TLC) which is analyzed as a glucose and galacto

دراسة لتشخيص بكتريا الملوية البوابية Helicobacter pylori المعزولة من الخزع النسيجية والغائط == Study For Diagnosis Helicopacter Pylori Isolated From Biopsy And Stool

Author name: ابرار علي حسين
Supervisor name: هيام عبد الرضا كريم العواد | ياسمين خضير الغانمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Kerbala - College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Karbala
First pages:
Abstract: تناولت الدراسة الحالية عزل وتشخيص بكتريا (Helicobacter pylori) من الخزع النسيجية وعينات البرازالماخوذة من المرضى المراجعين مستشفى الحسين التعليمي حيث جمعت 122 عينة من الخزع النسيجية من وحدة الناظور من 61 مريض بواقع عينتين للمريض الواحد, كما تم جمع 228 عين | The present study addressed isolate and diagnose the bacteria (Helicobacter pylori) from biopsy and stool, samples taken from AL - Hussein Teaching Hospital where collected 122 samples of biopsy from the endoscopy. From 61 patients by two samples per patient, also were collected 228 samples of stool from the laboratory of the Department of parasites by two samples of 114 patients for the period from January 2014 to January 2015. Where shown on biopsy results which were subject to examination by the rapid urease test (RUT) the presence of 60 (98.4%) cases, while there were 23 (37.7%) case of those bacteria being positive related to biopsy was not significant differences between the two tests performed. When the distribution of test results (RUT) and culture according to age group, it appear that the highest percentage of infection was in 50 - 41 age group that which showed the highest percentage on examination (RUT) of the number of infected at 16 (26.23%) cases, either in the culture technique number of infected at 9 (14.75%) cases. But when the results of each of the examination (RUT) and culture were distribution according to the sex of the patient's infection was the higher in the females than males reaching males 28 (45.90%) cases in females of 32 (52.46%) cases for examination (RUT). No significant differences appear between the sexes in terms of infected. While the males in 8 (13.11%) and in the case of females was 15 (24.59%) case for the culture technique it did not show significant differences between the sexes in terms of the patients. While taking stool samples to check the stool antigens, it was found that there are 76 (66.7%) cases diagnosed while the technology for the polymerase chain reaction gene cagA to 49 (43%) cases have significant differences between the tests performed. When distribution the results of each of the stool antigens and polymerase chain reaction technique by age group category the highest infected was in 46 - 55 the age group with the number of 23 (20.18%) people case for screening stool antigens. The test results of the technology polymerase chain reaction showed that highest rate of infection was in the 35 - 26 age group where the number of patients was at 15 (13.16%) case.The results of the stool antigens examination also showed that the infection rate among males was higher than females, 39 males (34.21%) and in the case of females has reached the number of 37 cases (32.46%) casesThat did not show significant differences between the sexes patient. The technical results of the polymerase chain reaction by sex has been the injury was higher in females than males reaching 22 in males (19.30%) cases, while in females was 27 (23.68%) cases did not show significant differences between the sexes in terms of the infected. The results of the study conclude that (RUT) and stool antigen test is the fastest in the detection of infection from the bacteria H. pylori and then comes beyond culture technique for biopsy sample and then polymerase chain reaction for stool samples