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تاثير المضادات الحيوية على الاستجابة المناعية في مرضى التهاب السبيل البولي

Author name: سلطان كريم سلطان

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

University location: Karbala

Key words:

الامراض التناسلية 2 - السيلان

دراسة مقارنة للعلاقه بين معدل النبض ووزن الجسم والعظام والعضلات في بعض الفقريات

Author name: نصير مرزة حمزة الزبيدي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

University location: Karbala

Key words:

الحيوان - علم 2 - الفقاريات

انتاج وتوصيف انزيم الكلكواميليز من عزله محليه للفطر (اسبيرجلس)

Author name: جمان خليل ابراهيم

General topic: Biology

Specific topic: Life Science

Degree: Higher Diploma

University: University of Kerbala

University location: Karbala

Key words:

الفطر - نبات 2 - رسائل جامعية

دراسة مقارن بين طريقتي تقنية (الدحرجة والطبع) لاخذ بصمات الاصابع

Author name: زينب سالم جعفر الكاظمي

General topic: Biology

Specific topic: Life Science

Degree: Higher Diploma

University: University of Kerbala

University location: Karbala

Key words:

بصمات الاصابع

تقدير الكولوكاكون شبيه بيبتايد (1) والبروتين جي المزدوج المستقبل لدى مرضى السكري في العراق والمصابين لفرط وقصور الغدة الدرقية

Author name: هدى فرحان احمد

General topic: Biology

Specific topic: Life Science

Degree: Doctorate

University: University of Kerbala - College Of Science - Department Of Biology

University location: Karbala

بعض العلامات المناعية والجزيئية للقرحة المعدية == Some Immunological and Molecular Markers of Peptic Ulcer

Author name: محمد باقر شهيد عبد الزهرة الخطيب

Supervisor name: كوكب عبد الله حسين السعدي | حسن علي حسين

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: English

University location: Karbala

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دراسة مقارنة لطرق تشخيص طفيلي المشعرات المهبلية وتحديد بعض المعايير المناعية لدى النساءالمصابات بداء المشعرات المهبلية == Comparative study of diagnosis methods of Trichomonas vaginalis and determine some immunological marker among Trichomonasis infected women

Author name: زهﺮاء زهﯿﺮ ﻣﺤﻤﺪ المعموري

Supervisor name: جميل جري الحميداوي | علي عطية عبد الحسناوي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: English

University location: Karbala

First pages:

دراسة بيئة مجتمع الطحالب الملتصقة على نباتي القصب والشمبلان في نهر بني العراق - حسن(نهر الفرات) /كربلاء المقدسة == Environmental study for Epiphytic Algal community on two macrophytes in Bani - Hassan River(Euphrates River) /Holy Kerbala city - Iraq

Author name: محمد جاسم محمد الربيعي

Supervisor name: عبد عون هاشم علوان الغانمي | حسن جميل جواد الفتلاوي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/06/Rp-Environmental-study-for-Epiphytic-Algal-community-on-two-macrophytes-in-Bani-Hassan-RiverEuphrates-River-Holy-Kerbala-city-Iraq.pdf

University location: Karbala

First pages:

الكشف عن جينات السموم المعوية وبعض العوامل المؤثرة على السموم المعوية لعزلات المكورات العنقودية الذهبية في مدينة كربلاء == Detection of Enterotoxin genes and some of Enterotoxin influencing factors of Staphylococcus aureus isolates in kerbala city

Author name: هبة مهدي محمود موسى

Supervisor name: جاسم محمد مصطفى | مهند محسن احمد

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: English

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/07/Rp-Detection-of-Enterotoxin-genes-and-some-of-Enterotoxin-influencing-factors-of-Staphylococcus-aureus-isolates-in-kerbala-city.pdf

University location: Karbala

First pages:

توصيف بعض الفطريات المسببة للاخماج الجلدية وعلاقتها ببعض المعايير المناعية والفسلجية == Identification of some Fungi causing skin infection and relationship with some immunological and physiological criteria of Tinea patients in holy kerbala province

Author name: ناصرة حمزة جبير البركات

Supervisor name: زهير حميد عبود الظويهري | جاسم حنون هاشم العوادي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

University location: Karbala

First pages:

تحري عن جين الفوعه usp وتكوين الغشاء الحيوي لبكتيريا Escherichia coli المعزولة من الادرار == Investigation of the virulence gene USP and biofilm formation for Escherichia coli isolated from urine

Author name: حسين علي محسن الجبوري

Supervisor name: هيام عبد الرضا كريم العواد

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Karbala

First pages:

دراسة مناعية في مرضى الذئبة الحمراء الجهازية الذين يشكون من الفشل الكلوي المزمن == Immunological Study in Systemic Lupus Erythoromatosus Patients Complaining from Chronic Renal Failure

Author name: نور هاشم ماشاء الله الهاشمي

Supervisor name: هادي رسول حسن المسعودي | محمد صالح مهدي الكردي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science

Language: English

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/07/Rp-Immunological-Study-in-Systemic-Lupus-Erythoromatosus-Patients-Complaining-from-Chronic-Renal-Failure.pdf

University location: Karbala

First pages:

دراسة بعض الدلائل الفسلجية والكيموحيوية في الاشخاص المصابين بمتلازمة الايض في محافظة كربلاء == Study of Some Physiological and Biochemical Markers in Metabolic Syndrome Patients in Kerbala Province

Author name: حيدر بخيت عباس الكريطي

Supervisor name: ستار جاسم حتروش | فاضل جواد ال طعمة

General topic: Biology

Specific topic: Life Science

Degree: Doctorate

University: University of Kerbala - College Of Education For Pure Sciences

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/07/Rp-Study-of-Some-Physiological-and-Biochemical-Markers-in-Metabolic-Syndrome-Patients-in-Kerbala-Province.pdf

University location: Karbala

First pages:

تاثير المستخلص الكحولي لنبات الزنجبيل على تكوين الغشاء الحيوي لبكتريا Streptococcus mutans المعزولة من مرضى تسوس الاسنان == Effect of alcoholic extract of Zingiber officinale on biofilm formation of Streptococcus mutans which isolated form dental caries patients

Author name: ازهار راقب كاظم الفتلاوي

Supervisor name: هيام عبد الرضا كريم العواد

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2022/08/Rp_Effect-of-alcoholic-extract-of-Zingiber-officinale-on-biofilm-formation-of-Streptococcus-mutans-which-isolated-form-dental-caries-patients.pdf

University location: Karbala

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مؤشرات الدم و حالة الحديد في مرضى الفشل الكلوي في محافظة بابل == Haematological indices and iron status in renal failure patients in Babylon Governorate

Author name: حسين حازم مدب الغانمي

Supervisor name: وفاء صادق الوزني | نجلاء بدر العوادي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/07/Rp-Haematological-indices-and-iron-status-in-renal-failure-patients-in-Babylon-Governorate.pdf

University location: Karbala

First pages:

تاثير بعض المضادات الحيوية في تثبيط عدد مـن عوامل الضراوة لبكتريا Pseudomonas aeruginosaوProteus mirabilis المعزولة من خمج المسالك البولية == Effect of some antibiotics on inhibition of some virulence factors of pseudomonas aeruginosa and proteus mirabilis isolated from urinary tract infection

Author name: حنين زهير علي الغانمي

Supervisor name: ذكرى عدنان جواد المسلماوي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

University location: Karbala

First pages:

تقييم فعالية بعض المضادات الحيوية ضد بكتريا العنقوديات الذهبية المسببة لانتان الدم و تاثيرها على بعض المعايير الالتهابية للمرضى في كربلاء المقدسة العراق == Evaluation of some antibiotics activity against Staphylococcus aureus causing septicemia and some inflammatory parameters in patients in Holy Karbala Iraq

Author name: ايات علاءالدين عبد العزيز ال طعمة

Supervisor name: ذكرى عدنان جواد المسلماوي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Karbala

First pages:

تطور ظهور صفة المقاومة في حشرة الذبابة المنزلية Musca domestica Linnaeus (1758) (Diptera : Muscidae) باستعمال منظمي النمو Trigard و Match == Development Occurrence of Resistance Feature in House flies Musca domestica Linnaeus (1758) (Diptera : Muscidae) Two Growth Match and Trigard

Author name: الاء سجاد صيهود حسين ا لخفاجي

Supervisor name: رافد عباس علي العيسى

General topic: Biology

Specific topic: Life Science

Degree: Doctorate

University: University of Kerbala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Karbala

First pages:

النمط المناعي للاشخاص المعرضين للحروق والمصابين ببكتريا الزوائف الزنجارية (Pseudomonas aeruginosa) وتوصيفها الجزيئي لمقاومة المضادات الحياتية == Immunological profile of burned patients infected with Pseudomonas aeruginosa and Molecular characterization of It's Antibiotic Resistance

Author name: ضواء محمد صلاح الخطيب

Supervisor name: وفاء صادق الوزني | ياسمين خضير الغانمي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science

Language: Arabic

University location: Karbala

First pages:

Abstract: هدفت الدراسة الحالية الى بيان نسبة تردد بكتريا الزوائف الزنجارية Pseudomonas aeruginosa بين مرضى الحروق في محافظة كربلاء. مع بيان بعض التغايرات المناعية المرافقة لاصابات الحروق في جسم المصاب وبعض الصفات الوراثية لبكتريا المعزولة التي تجعلها مقاومة للعديد من المضادات الحيوية الشئ الذي يزيد من زيادة نسبة حالات الانتان الدموي والموت بين هؤلاء المرضى . قد تم في الفترة من شهر شباط2014 الى شهر اب 2014 جمع مسحات جلدية وعينات دم من 64 عزلة من مرضى الحروق الراقدين في مستشفى الحسين مع جمع 30 مسحة بيئية من ردهات الحروق نفسها في تلك المستشفى ،اذ ظهر ان 45 % من مسحات الحروق كانت تعود لبكتريا الزوائف الزنجارية في حين 26.1% فقط كانت نسبة عزل هذه البكتريا من المسحات البيئية ولبيان العلاقة بين عمر المريض ودرجة الحرق ونوعه تبين ان نسبة الحروق 60.9) %) في الاعمار دون 15 سنة كانت اعلى من نسبتها 39.11)%) في الاعمار فوق 15 سنة . في حين كانت الحروق الناتجة عن السوائل الحارة لدى المصابين دون 15سنة اعلى معدلات الحرق بينما حروق اللهب شكلت النسبة الاكبر (29.7%) في الاعمار فوق 15سنة بالاضافة الى ان الاناث كانت الاكثر عرضة للحرق ونسبته (56.3%) مقارنة بالرجال (%43.8). كانت بكتريا P.aeruginosa المعزولة عالية المقاومة لاغلب المضادات الحيوية حيث كانت مقاومة لكل من )Cefatotaxime 88.6%) , Ceftazidime (85.7%), Oxacillin (71.4)و Meropenin(85.7%) في حين وصلت مقاومتها الى (%77.1) للمضاد الحيوي Gentamycin . اذ كانت اغلب العزلات مقاومة لاكثر من مضاد حيوي واحد لبيان سبب هذه المقاومة العالية تم الكشف وراثيا عن جينات البيتالاكتميز مثل per - 1 وoxa - 10باستخدام تقنية تفاعل تضخيم الدنا (PCR) حيث اظهرت نتائج البلمرة بحجم 760bp بالنسبة لجين المسؤول عن انزيم oxa - 10 وناتج بلمرة بحجم 927bp للجين المسؤول عن انزيم per - 1 ، وكانت نسبة تواجد جين oxa - 10 بنسبة 85% من مجموع العزلات الكلي اما الجين per - 1 فقد تواجد بنسبة 50% من العزلات المحصلة عليها.اما نتائج الدراسة المناعية الخاصة لبيان تاثير الحروق بانواعها على فعالية الجهاز المناعي للمريض فقد ظهر جليا ارتفاع واضح لكريات الدم البيض عند المصابين والذي يتناسب طرديا مع شدة الحرق فقد وصلت اعداد الخلايا mm3) / 103) 10.44 , 13.222 و15.955 لكل من مجموعة مرضى الحروق البسيط والمتوسطة والشديدة على التوالي حيث كان الارتفاع بصورة خاصة لكريات الدم البيض العدلة مقارنة ببقية الانواع الخلوية .اما المناعة الخلطية المتمثلة بتراكيز كل من بروتين الطور الحاد والمتمم فقد لوحظ ارتفاع معنوي في قيم بروتيات الطور الحاد والذي يتزايد طرديا مع شدة الحرق ليصل الى 63.4 ملغم/لتر في الحروق البسيطة في حين وصل الى) 95.2 و121.8 ) ملغم/لتر لكل من الحروق المتوسطة والشديدة على التوالي .اما تراكيز بروتين المتمم C3 فقد ظهرت التحليلات الاحصائية ارتفاع غير معنوي بمستوى المتمم الثالث لكل درجات الحروق البسيطة وبنسبة 125.45 ملغم/ديسيلتر,المتوسطة137.5 ملغم/ديسيلتر والشديدة 140.15 ملغم/ديسيلتر مقارنة مع الاشخاص الاصحاء 122.32 ملغم/ديسيلتر. في حين كان الارتفاع معنويا ( p≤0.05) في قيم الحركي الخلوي IL - 6 ليصل الى 118 بيكوغرام /مل, 86 بيكوغرام /مل و33 بيكوغرام /مل كل من مرضى الحروق البسيطة والمتوسطة والشديدة على التوالي .على العكس من ذلك فقد سجلت عامل التنخر الورمي TNF اعلى ارتفاع في مرضى الحروق الشديدة اذ بلغ 600.45 بيكوغرام/مل مقارنة بالاشخاص الاصحاء 175.15 بيكوغرام/مل. | This study aimed to a statement the ratio Pseudomonas.aeruginosa between burns patients in the Al - Hussein Hosppital in Kerbala city and as well as some variations of immune system that associated with injuries or burns in the body patient in addition explain some genetic characters of isolated bacteria that make them resistant to different types of antibiotics, which increases sepsis & death among these patients. Skin swabs and blood samples were collected from 64 burn patients admitted to the hospital in period febrwary 2014 to agust 2014 in addition 30 swabs from environmental hospital also collected . Pseudomonas aeruginosa was isolated from burns swabs (45% ) while only 26.1% were isolated from environmental swabs. Relationship statement between the patient's age and the degree, type of the burn showed that the percentage of burns ( 60.9%) in the ages under 15 years higher than the rate of (39.11%) in ages over 15 years .The burns rates caused by hot liquids in patients under 15 years than flame higher burn while burns flame formed the most (29.7%) in the ages over 15 years . That females were most likely to burn rate (56.3%) compared to men (43.8%) P.aeruginosa were isolated high resistance to most antibiotics used where the resistance of each Cefatotaxime (88.6%), Ceftazidime (85.7%),Oxaciilin(71.4) and Meropenin (85.7%) while resistant to (77.1%) for Gentamycin. The cause of multidurg resistance was detected genetically by determined B - lactamase genes of per - 1and oxa - 10 using a technique amplified DNA reaction (PCR). The results showed polymerization Size 760bp for the gene responsible for oxa - 10 and the result Size polymerization 927bp gene responsible for an enzyme per - 1the presence of oxa - 10 (85%) in the total isolates either per - 1gene has presence by (50% )in the isolates collected them.The immunological results showed clearly increased white blood cells that directly proportional to the severity of the burn has reached the preparation of cells (10.44, 13.222, 15.955 ) 103/mm3 of the patients with simple and moderate to severe burns group respectively, where the rise, in particular, to neutrophil white blood cells compared to other types of cells.The immune humoral concentrations of both protein acute phase and complement were noted a significant rise in acute phase protein values and which are increasingly proportional to severity of the burn to arrives 63.4 mglL in simple burns while arrived( 95.2 and 121.8) ) mglL for each of degrees burns moderate and severe on respectively.It was found significant increase in the acute phase protein values and which increased directly according to the severity of the burn for up to 63.4mg/ml in simple burns while reached( 95.2 and 121.8)mg/ml each of the moderate and severe burns respectively.Statistical analyzes of complement protein C3 appeared insignificant rise in the levels of the complement for each degree of burns simple 125.45, medium (137.5 mgldL)and severe (140.15 mgldL) compared with healthy people (122.32 mgldL).While the rise significant at the level of probability (p ≤0.05) in the kinetic values cellular IL - 6 to reach( 118 pg / ml), (86 pg / ml), (33 pg / ml) each of the patients simple burns and moderate to severe respectively. On the opposite it values of kinetic cellular TNF were recorded the highest rise in patients with severe burns, reaching 600.45 (pg / ml) compared to control

تحضير مركبين نانويين هجينين من الكلورهكسدين وحامض التانيك وتحديد فعلهما المضاد للاحياء المجهرية المعزولة من مرضى الحروق في محافظة كربلاء المقدسة == Preparation of Two Nanohybrid Compounds From Chlorhexidine And Tannic Acid And Determination Their Antimicrobial Activities Against Microorganisms Isolated From Burns Patients In Sacred Karbala Province

Author name: نورس مجيد حميد الخفاجي

Supervisor name: علي عبد الكاظم الغانمي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/07/Rp-Preparation-of-Two-Nanohybrid-Compounds-from-Chlorhexidine-and-Tannic-Acid-and-Determination-Their-Antimicrobial-Activities-Against-Microorganisms.pdf

University location: Karbala

First pages:

Abstract: تضمنت هذه الدراسة عزل وتشخيص اهم انواع البكتريا المسببة لاخماج الحروق كما تضمنت ايضا تحضير وتشخيص مركبين نانويين هجينين احدهما من المطهر كلورهكسدين والاخر من حامض التانيك. تم تقييم الفعالية التثبيطية للمطهر النانوي المحضر اعلاه ضد البكتريا المعزولة في هذه | This study included the isolation and diagnosis of the most important species of bacteria responsible for the infection in burns. It also included the preparation and detection of two hybrid nano compounds, one of them from the disinfectant chlorhexidine and the other from the tannic acid. The inhibitory activity of the nano disinfectant was assessed against the isolated bacteria in this study also, the antioxidant activity of the hybrid nano tannic acid was identified and the results showed the following : 1 - As a total 32 samples were taken from burned patients hospitalized in the burning ward Imam Al - Hussien medical city in Sacred Karbalaa governate. It had been obtained 8 isolates (34.78) % from Pseudomonas spp., 7 of them were Pseudomonas aeruginosa and one was P. oryzihabitans , 4 isolates (17.39) % were Acinetobacter baumannii, 4 isolates (17.39) % were Klebsiella pneumoniae, and one isolate (4.34) % of Proteus mirabilis, Escherichia coli, Serratia liquefaciens and Chryzeobacterium meningosepticum and two isolates (8.69) % of Staphylococcus aureus in addition to one isolate (4.43) % from Candida guilliermondii yeast.2 - Chlorhexidine disinfectant and tannic acid were used in preparation of the two hybrid nano compounds after intercalating each of them on layers of double hydroxide (Mg/Al - LDH). FT - IR spectrum studies has shown the appearance of specific chemical groups and the disappearance of others while the spectrum of x - ray(XRD) has shown the appearance of new levels of diffraction in the spectrum of the hybrid nano disinfectant Mg/Al - CHX - LDH and the spectrum of the hybrid nano tannic acid Mg/Al - TA - LDH as compared to the spectrum of the Mg/Al - LDH, which indicate that the two prepared compounds under study is a nano hybrid compounds. Results of the scanning electronic microscope has shown, the change in the shape of the surface of the nanohybrid compounds that prepared above as compared to the layer of double hydroxide. It turns out from the results of the examination with the use of Atomic Force Microscope (AFM), that the diameter of the particles of hybrid nano disinfectant and hybrid nano tannic acid were (121.66 and 77.70) nm, respectively. 3 - The inhibitory activity of the hybrid nano disinfectant Mg/Al - CHX - LDH and the free disinfectant (Free CHX) has been studied and the results revealed followings : a. The hybrid nano disinfectant has the highest inhibitory activity against the two bacterial isolates P. aeruginosa (11 and 23 - A) with an inhibition diameter of 24 mm for each, while the inhibition diameter for the free disinfectant was 23.5 mm against the isolate P. aeruginosa 4 - C.b. The hybrid nano disinfectant showed the highest inhibitory activity against the two isolates A. baumannii (16 - A and 31 - A) with an inhibition diameter of 18 mm for each, while the highest inhibition activity for the free disinfectant was against the isolate A. baumannii 22 - B with an inhibition diameter of 19.5 mm.c. The hybrid nano disinfectant showed the highest inhibitory activity against the isolate K. pneumoniae (16 - B) with an inhibition diameter of 24.5 mm, while the highest inhibition activity for the free disinfectant was against the isolate K. pneumoniae 15 - B with an inhibition diameter of 24.5 mm also.d. The highest inhibition activity for the hybrid nanoic disinfectant against each of bacteria P. mirabilis and E. coli with an inhibition diameter of 23.5 mm, while the highest inhibitory activity for the free disinfectant was against the bacteria E. coli with an inhibition diameter of 24.5 mm, while the bacteria C. meningosepticum was the least affected among the other types of the isolated bacteria with an inhibition diameter of 8 mm and it was resistance to the hybrid nano disinfectant.e. The highest inhibition activity for the hybrid nano chlorhexidine was against the isolate S. aureus 21 with an inhibition diameter of 28.5 mm, while the highest inhibition activity for the free chlorhexidine was against the isolate S. aureus 22 - A with aninhibition diameter of 31.5 mm.f. The inhibitory activity of both the hybrid nano and the free disinfectant against C. guilliermondii yeast was closely to each other with an inhibition diameter of (28 and 29) mm, respectively.4. The study of antioxidant activity confirmed the following : a. Using free radical scavenging method, the IC50(Half maximal inhibitory concentration) value against ABTS free radical were at 10 µg /ml for free tannic acid while it was at 125 µg /ml for the hybrid nano tannic acid Mg/Al - TA - LDH.b. Measurement of ferric ion reducing power revealed that free tannic acid had reducing power greater than that of the hybrid nano tannic acid

دراسة تاثير الذيفان حال الدم الفا المستخلص من بكتريا Echerichia.coli في فعالية واستجابة الخلايا المناعية == Study The Effect of The Alpha Hemolysin Toxin Extracted From Echerichia Coli In The Activity And Response of Immune Cells

Author name: وقار عدنان حمدان الكبيسي

Supervisor name: ذكرى عدنان جواد المسلماوي

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

University location: Karbala

First pages:

Abstract: تضمنت الدراسة الحالية محورين الاول الحصول على عزلات من بكتريا E.coli من المرضى المصابين بخمج السبيل البولي اذ تم الحصول على اربعة عزلات من بكتريا E.coli من مختبر الصحة العامة في كربلاء، وعزلة من بكتريا E.coli من براز شخص سليم من مستشفى الاطفال في كربلاء و| The isolation of E.coli bacteria from patients with urinary tract infection, Four isolates were obtained from the public health laboratory in Karbala, and isolation of E.coli bacteria from the feces from healthy person and S.aureus from the public health laboratory, on the basis of microscopic diagnosis and biochemical test isolates were confirmed diagnosis.This axis also included the investigation of the ability of four UTIs isolates to produce the toxin qualitatively on blood agar and measuring the diameters of hemolysis and quantitatively by measuring the amount of hemoglobin released from the RBCs.The toxin (hemolysin) was extracted from the most efficient isolate, after the cultivation in the chemically defined medum (CDM) The highest dilution gave hemolytic activity on blood cells 1/32 (320 units / ml), but after sedimentation, dialysis and freeze - drying found that (1 / 64) caused hemolysis of 50% of the blood cells compared to the standard curve.The results of this study, indicated that the role of the toxin (Hemolysin) in the pathogenecity and virulence of E.coli, lowered the lethal dose (LD50) of the bacteria which decreased from (107 × 3.16 cells / mL) to (106 × 2.34 cells / mL).The second axis included the study of the immune responce included : Studying the effect of the toxin on the immune cells isolated from the peritoneum of the mice through its effect on viability of these cells, using the concentrations of the toxin that showed the hemolytic activities (100, 90.70, 50.25) %, as well as determine the effect of the toxin on the phagocytosis S.aureus process in different periods The study found that the toxin effect on the immune cells depends on the concentration used.The low concentrations of the toxin, which showed hemolytic activity (25%) did not affect the viability of immune cells and their ability to phagocytosis where the results of the statistical analysis showed no significant difference (0.05 <p) in comparison with control, while high concentrations of the toxin showed effects on the viability of immune cells and on phagocytosis where statistical analysis significant difference (0.05> p) compared to control treatment, and the results showed that the effect of the toxin on the phagocytosis depended on the concentration and time, where phagocytic Index decrease with dose and time.It was estimated the level of cytokins (IL - 1B, IL - 2, IL - 6, TNF) in plasma were estimated for mice injected with sub lethal dose 50 (106) of the non hemolytic E.coli isolated from stool and another group which injected with LD50 of the toxin (25%) and a third group were injected with a mixture of bacteria the toxin in addition to the control group injected with normal saline, The level of cytokins during different periods of time (6, 12, 24, 48, 72) hour were estimated, where it was found that the sub LD50 of the non hemolytic E.coli had no effect on the level of TNF while sub LD50 of the toxin had no significant effect as well as the doses of the mixture in comparison with control treatment while the other cytokins, Results showed little rise in the level of IL - 1? in mice injected with non hemolytic bacteria and when the injection with sub LD50 as well as two doses mixture was large, as well as the case for IL - 2 and the IL - 6, but in different proportions, from this we conclude that the toxin stimulates the liberation of the IL - 1 and IL - 2 and the IL - 6 from immune cells, but does not affect the TNF.

دراسة جزيئية ومناعية لطفيلي اللشمانيا الجلدية Cutaneous Leishmania في المحافظات الوسطى والجنوبية == Molecular And Immunological Study of Cutaneous Leishmania In The Middle And Southern Provinces

Author name: ازهار موسى جعفر الموسوي

Supervisor name: علي حسين الكبيسي | مهدي حسين العمار

General topic: Biology

Specific topic: Life Science

Degree: Doctorate

University: University of Kerbala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/06/Rp-Molecular-and-Immunological-study-of-Cutaneous-Leishmania-in-the-middle-and-southern-provinces-.pdf

University location: Karbala

First pages:

Abstract: The study includes distribution of the Cutaneous Leishmania in some governorates in Iraq. Specimens are included cases of outpatients in hospitals like : Al - Karamah, Al - Sadir Teaching Hospital in Baghdad, Al - Hilla Teaching Hospital, Al - Hussein Teaching Hospital and Ein Altamer General Hospitals in Kerbala, Al - Sadir Teaching Hospital in Al - Najaf, Al - Karamah and Al - Zahraa Teaching Hospitals in Wasit, Al - Diwanyia General Hospital. Al - Hussein General Hospital in Al - Nasiriya, Al - Smawa General Hospital, Al - Sadir Teaching Hospital and Al - Qurna General Hospital in Al - Basrah during October 1122 to march 1121 Cutaneous Leishmania is consider a major problem that faces the patients because it causes deformities in the infection region. The study includes 330 cases of skin ulcer in which 225 of them were microscopically positive. Parasitic growth was done using two types of cultural media NNN and RPMI - 1640.The results have shown a considerable difference between male and female in which male was significantly exceeding 131) %22.11 (and female was 94) %72.44 (and 139) %72.44 (of outpatients from rural areas increased in numbers from outpatients of urban areas, and only) %12.11(from urban areas.The number of ulcerations in body has exceeded infection, that means more than one ulceration in male 83 (85%) and for female 41 (62%), and concerning infection in three or four ulceration, it is approximately equal and normally distributed on face areas 99 and a percentage of (44%) then lower arms are 92 and a percentage of (40.80%) and the upper parts are 33 and a percentage of (14.66%). Also, one infection was recorded on The study shows that 46 patients in a percentage of (%20.44) having a dry infection; whereas 179 patients (%79.55) having the wet type.For accurate diagnosis of Leishmaniaa species distributed in Iraq, PCR Was used technique and diagnosed two types of Leishmania parasite that causes the illness. A band in a length of 560 bp in 186 samples related to Leishmania major and the band sample was 750 bp related to 39 Leishmania tropica.Through studying the samples in Kerbala City, the number infected samples were 125 for the years of 2010 - 2011 the actuality is (73) infection and (52) infection from 2011 - 2012 in a ratio of (58.4 % and % 72.7 accordingly) in different inhibited areas in the governorate in which (51)) %71.2 (was in Ein Al - Tamir Suburb, and 29 cases of) %11.1 (in Al - Husseinya Suburb, as well as 17 cases of (13.6%) in Al - Hur Suburb, 16 cases) %21.2 (in the south quarters and 7 cases of (5.6%) in the north quarters and only 5 cases of (4%) in the city center.The molecular diagnosis in Polymerase Chain Reaction for Kerbala governorate samples have shown 31) %17.2 (of L.tropica, 22) %24.7(of them were male and 9 of) %4.1 (of them were female. L. major was 94 of) %42.1 (in which 55 of) %77 (were in male and 39 of) %12.1 (were in female.The immunological study for the patients serums with L. major by ELISA technique has significantly shown raised values of IgG and IgM during infection in comparison with the control group, then it declines slowly after treatment in which IgG was in ratio (1811.1 ± 523.1 mg/dl), and IgM was in ratio (166.7 ±23.6 mg/dl). Also, in L. tropica - IgG was in ratio (1722.1 ± 524.0mg/dl) and IgM was in ratio (25.3±182.9mg/dl) in comparison with control specimens, and then the ratios have declined after treatment dosages with Pentostam drug.The cellular dynamics values have shown an abstract increase in which interferon - gamma (IFN - ?) in infected patients with cutaneous L. major were (221.1±2.2mg/dl) which declined after treatment abstractly to (7.12±1.1mg/dl) as well as for L tropica (22.1±7.2mg/dl) which shows no significant abstract difference after treatment with control.An increase appears in cytokine IL - 10 that reached in L.major (122.1±8.2mg/dl) and after treatment was (8.11±2.2mg/dl) and in L. tropica was (222.1±2.2mg/dl) with no significant abstract difference after treatment with control specimen.As a simple attempt to find a vaccine for Leishmania disease a Lipophosphoglycan was isolated and purified as known factor for promastigot stage. The vaccine injected for two groups of Bulb mice in a concentration of purified vaccine (L.majorand Ltropica) to study the immunological response by detecting the Lymphocyte Transformation assay, the Delayed Type Hypersensitivity test and the PhagocytosisIndex.In lymphocyte transformation the ratio of L. tropica was % 7.2 and for L. major was % 21.7 and there is significant difference by P? 0.05 in comparison with control specimen in which its ratio was %7.4, and for Delayed Hypersensitivity test study the average foot thickness injected with vaccine and the other foot which injected with phenol - saline solution - only (1.11 ± 2.12ml) for the first group and (1.21 ± 2.72) for the second group, and for the control group it has registered (1.77± 1.15 ml) with a considerable difference of P? 0.05. By calculating the ratio of Phagocytosis Index cells, it was 17% and 27.6% for the first and second groups while for the control group it has been reaching 9.10%. Therefore, we can get advantage from these values as indicators for the rule of vaccine in the immunological response and triggering of vaccine idea for this disease.

تاثير المشتت الحيوي لبكترياspp. Lactobacillus على عوامل الضراوة لبعض البكتريا السالبة لصبغة غرام المعزولة من الاسهال == Effect Of Lactobacillus Spp. Biosurfactant On Virulence Factors Of Some Gram Negative Bacteria Isolated From Diarrhea

Author name: جنات كمال الخزرجي

Supervisor name: وفاء صادق الوزني | ناجح هاشم كاظم

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: English

University location: Karbala

First pages:

الفعالية الحياتية لعدد من المستخلصات النباتية اتجاه بعض الفطريات المعزولة سريريا == Bioactivity of Some Plant Extracts On Some fungi Isolated Clinically

Author name: نور شاكر مهدي الوزني

Supervisor name: زهير حميد عبود | ابراهيم خليل حسون

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

Full text from the source: https://uokerbala.edu.iq/wp-content/uploads/2020/07/Rp-Bioactivity-of-Some-Plant-Extracts-On-Some-fungi-Isolated-Clinically.pdf

University location: Karbala

First pages:

دراسة تاثير الزيوت الطيارة المستخلصة من بعض النباتات في بكتريا Pseudomonas aeruginosa المعزولة من خمج الحروق == Study the effect of volatile oils extracted from some plants on Pseudomonas aeruginosa isolated from burn infection

Author name: لمى عدنان مزعل

Supervisor name: ذكرى عدنان جواد | ابراهيم صالح عباس

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Kerbala - College Of Science - Department Of Biology

Language: Arabic

University location: Karbala

First pages: