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دور اضافة البلازما الغنية بالصفيحات الدموية لتنشيط النطف مع الترشيح بالصوف الزجاجي وقياس جودة وحيوية النطف في الرجال غير الخصبين == Beneficial Role of Adding Platelet - Rich Plasma (PRP) to the Medium used for Glass Wool Filtration Technique Assessing best Recovery of Spermatozoa in Infertile Men

Author name: وطفاء اسعد عبد الجبار عبد الكريم
Supervisor name: حيدر علي لفتة | مؤيد سريبت عبود
General topic: Biology
Specific topic: Zoology
Degree: Master
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies - Department of Life Sciences
Language: English
University location: Baghdad

دور تعدد اشكال جين مستقبلة الهرمون المنبه للجريب في امراض متلازمة المبيض المتعدد الكيسات في النساء العراقيات == The Role of Follicle Stimulating Hormone Receptor Gene Polymorphisms in Pathogenesis of Polycystic Ovary Syndrome in Iraqi Women

Author name: زينب كاظم عبد
Supervisor name: استبرق عبد الرسول الواسطي | بان عباس عبد المجيد
General topic: Biology
Specific topic: Assisted Reproductive Laboratory Techniques
Degree: Doctorate
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies
Language: English
University location: Baghdad
First pages:

مستوى هرمون الليبتين في مصل النساء المصابات بمتلازمة تكيس المبايض قبل وبعد العلاج بالميتفورمين == Serum Leptin Level In Women With Polycystic Ovary Syndrome Before And After Treatment With Metformin

Author name: بتول حسين كاظم
Supervisor name: وليد حميد يوسف سندال النصيري
General topic: Biology
Specific topic: Biotechnologies
Degree: Higher Diploma
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies
Language: English
University location: Baghdad
First pages:
Abstract: Polycystic ovary syndrome is the most common endocrinopathy in women, affecting 5 - 10% of women of reproductive age.The principal features of PCOS are anovulation, resulting in irregular menstruation, amenorrhea, ovulation - related infertility, and polycystic ovaries; excessive amounts or effects of androgenic hormones. Leptin, a key hormone in energy homeostasis and neuroendocrine function, has a permissive role in the pathogenesis of reproductive dysfunction. Aim of the study To assess the role of serum leptin in women with PCOS and to evaluate leptin levels in PCOS women before and after treatment with metformin. Materials and Methods This prospective experimental study included 60 women of reproductive age (18 - 38years) were allocated to four groups : 15 obese women with PCOS (BMI >30 kg/m2), 15obese controls, 15 non - obese women with PCOS (BMI 18 - 30 kg/m2), and 15 non - obese controls. Serum leptin and insulin levels were measured and compared between case and control subjects also comparison done before and after treatment with metformin. Results There was a significant increase in leptin in non - obese PCOS group (8.2±2.73) compared to non - obese control (5.64±1.43), (P value=0.0032), insulin level was significantly higher in PCOS group (15.87±6.65) than control (5.47±1.68), (P value<0.001). There was significant decrease in BMI, leptin and insulin levels after 12 weeks of metformin treatment in obese and non - obese PCOS subjects. Conclusions Leptin level increased remarkably with increasing body weight. It is higher in non - obese PCOS women in comparison with non - obese healthy women. Treatment with metformin for 12 weeks resulted in significant reduction in leptin levels in both obese and non - obese PCOS subjects.

مقارنة نسب الحمل للنساء اللواتي يعانين من توقف الطمث لاسباب ثانويه بعد استعمال ادوية المنشطه للحويصلات المبيضيه باستخدام التمنيه داخل الرحم والطريقه الطبيعيه للحمل == Comparison of Pregnancy Rate Between Intrauterine Insemination And Spontaneous Pregnancy After Ovulation Induction In Women With Secondary Amenorrhea

Author name: سوسن كاظم عبد الامير
Supervisor name: محمد باقر محمد رشاد فخر الدين
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies
Language: English
University location: Baghdad
First pages:
Abstract: انقطاع الطمث لاسباب ثانوية هو انعدام الدوره الشهريه لمدة ثلاثة الى ستة اشهر للنساء اللواتي لهن دوره شهريه منتظمة.واستنادا لمنظمة الصحة العالمية ان انقطاع الطمث يتضمن مجموعة (WHOI) و(WHOII) والتي تكون (30%) من حالات العقم.وتعاني اكثر النساء ممن يكونون من م | Secondary amenorrhea refers to the lack of cycle for three months in a row or for more than six months in a woman who has had regular menstrual periods before, and its associated with conditions classified as World Health Organization (WHO) group 1 and WHO group 2 and has been estimated to represent almost (30%) of all causes of infertility. In clinical practice, WHO group 2 subjects present much more frequently than WHO group 1 subjects and they appear to be much harder to treat successfully. Among women classified as WHO group 2, those diagnosed with polycystic ovary syndrome (PCOS) constitute the largest group (60 - 85% of cases). These women account for most cases with oligomenorrhea (90%) and represent about (30%) of those with amenorrhea.ObjectiveThe aim of this study was to find out the pregnancy rate following intrauterine insemination (IUI) in women complaining of secondary amenorrhea after induction of ovulation and compare it with those achieve pregnancy by natural way.Material and MethodsSeventy five females with secondary amenorrhea were involved in this study, there ages were ranged between (?30, 30 - 39, ?40) years old with infertility duration ranged between (?2, 3 - 4, 5 - 6, 7 - 8, ?9) years. History and medical examination were done for all women, with tubal patency assessment by HSG or laparoscopy.By cycle day two, hormonal assay (S.FSH, S.LH, S.E2, S.Prolactin and S.Testosterone) were done for all females in addition to ultrasonography to exclude any ovarian pathology.Ovulation induction program was applied for all females which include either clomiphene citrate (CC) alone, recombinant Follicular Stimulating Hormone (r - FSH) alone or combined CC and r - FSH. Vaginal ultrasonography was done for all females on day 12 or13 to assess ovarian response (size and number of mature follicles) and endometrial thickness. Meantime all male partners undergo seminal analysis to assess sperm parameters (sperm concentration, sperm motility and morphology).Thirty eight of females participated for natural pregnancy and thirty seven of females participate for IUI. Washing swim up technique was used for sperm activation for all male partner involved in IUI group.Follow up of all these females for pregnancy test to assess the pregnancy rate for both groups (natural and IUI).ResultsThe pregnancy rate achieved by natural way for a women with secondary amenorrhea is (23.7%), and (18.92%) by IUI way.In this study, it was found that female age, infertility duration, hormonal balance and ovulation induction program affect the pregnancy outcome where the young age group and short infertility duration correlate positively with pregnancy rate. Furthermore, early diagnosis of underlying causes of secondary amenorrhea to achieve hormonal balance by suitable ovulation induction program, have an effect on pregnancy rate, were combined CC and r - FSH produce a significant result among females achieve pregnancy by natural way (13.5%) compared with other ways. While ovulation induction by r - FSH drugs produce a significant results (13.5%) among females achieve pregnancy by IUI way.ConclusionFrom results of this study, it was concluded that young female age, short infertility duration, early diagnosis of underlying causes of secondary amenorrhea and the use of r - FSH drugs for ovulation induction produce a high significant pregnancy rate using IUI program.

دراسة مقارنة بين تاثير سترات الكلوميفين وسترات التاموكسيفين على بعض معايير نطف الفئران == Comparative Study On The Effects of Clomiphene Citrate And Tamoxiphene Citrate On Some Sperm Parameters In Mice

Author name: رائد عبد الاله عباس
Supervisor name: محمد باقر محمد رشاد فخر الدين | محمد عوده سلمان
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies
Language: English
University location: Baghdad
First pages:
Abstract: يعد العقم واحدة من المشاكل الاجتماعية المهمة في معظم انحاء العالم.استنادا الى الاحصائيات الحالية حوالي 15% من المتزوجين يواجهون العقم. يؤدي تقييم السائل المنوي غير الطبيعي للاستروجينات الى تشخيص قلة اعداد النطف ونشاطها وشكلها الطبيعي ومن ناحية اخرى تؤثر | Infertility is one of the most critical social problems in the all of population world. According to the current statistics, about 15% of married couples face with infertility. Assessment of abnormal semen parameters leads to diagnosis of oligoasthenoteratozoospermia, and in others, it leads to isolated abnormalities of sperm concentration, motility, and morphology, due to the lack of differentiable or correctable etiology, Both clomiphene citrate and tamoxiphene citrate, as an anti - estrogen, is the most common prescribed medication for male infertility. In humans, ant estrogens interfere with the normal negative feedback of sex steroids at the hypothalamus and pituitary, thereby increasing endogenous gonadotropin releasing hormone (GnRH) secretion from the hypothalamus and follicle stimulating hormone (FSH) and luteinizing hormone (LH) secretion directly from the pituitary. Objective : The objective of this study was to investigate the effect of clomiphene citrate (CC) and tamoxiphene citrate (TC) administered to male mice on quality of sperm parameters.Materials and Methods : Ninety six male mice were divided into two major groups, and every one of these groups was subdivided into three minor groups involving control and two treated groups. In the CC study, 48 mice were administrated orally 0, 0.0125 mg and 0.025 mg as control (G 1), low dose (G 2) and high dose (G 3) for 42 days. While, in the TC study, 48 mice were administrated orally 0, 0.01 mg and 0.02 mg as control (G 1), low dose (G 2) and high dose (G 3) for 42 days. Then, the male mice were sacrificed, both vas deferens were collected and assessment sperm parameters involving sperm concentration, sperm motility and activity, and normal sperm morphology. Results : The results of present study appeared that the use high dose of CC or TC significantly increment (P<0.05) sperm concentration as compared to control groups. However, use high dose of CC significantly increment (P<0.05) percentages of sperm motility and normal sperm morphology. Meanwhile, the percentage of progressive sperm motility was increased significantly (P< 0.05) when low CC dose administered as compared to control and high CC dose groups. In this study, percentages of sperm motility, progressive sperm motility and normal sperm morphology showed best elevation when used low TC dose as compared to both control and high TC dose groups. High TC dose causes significant increment (P<0.05) in the percentage of sperm agglutination when compared to control and low TC dose groups.Conclusions : Based on the results of this study, it can be concluded that the administration of high dose of either CC or TC increases sperm concentration, while administration of low dose of either CC or TC enhances progressive sperm motility.

تاثير الادينوسين ثلاثي الفوسفات على اخصاب وتطور الاجنة الاولي خارج الجسم الحي في الفئران كنموذج للجنس البشري == Effect of Adenosine Triphosphate On In Vitro Fertilization And Early Embryonic Development In Mice As A Model For Human Being

Author name: مهند علي مشكور
Supervisor name: محمد باقر محمد رشاد فخر الدين | نوال خيري العاني
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies
Language: English
University location: Baghdad
First pages:
Abstract: يعد الاخصاب خارج الجسم الحي (IVF) من احد اهم التقنيات المستخدمة لانتاج اعداد كبيرة من الاجنة لبحوث التطور الاساسيه والبيولوجيا الجزيئية لنضوج البويضات المختبري والاخصاب. يعتمد الاخصاب خارج الجسم الحي وظروف حفظ الجنين المثلى على مكونات الوسط الزرعي كونها ا | In vitro fertilization (IVF) is one of the most important techniques used to produce large numbers of embryos for research into the basic development and molecular biology of in vitro oocytes maturation and fertilization. Optimal IVF and embryo culture conditions depend on the composition of culture media being the most important determinants of success in vitro interaction of the gametes and subsequent embryo development. Adenosine 5 - triphosphate (ATP) is a fundamental factor to maintain life, by providing energy, and controlling the cell function and metabolism. Objectives : The present study aims to investigate the effect of ATP supplied to SMART medium on a rate of fertilization and early embryonic development (ED) in mice as a model for human beings.Materials and Methods : The female mice (90) were superovulated using superovulation program to produce a large number of oocytes. These were divided into three groups, the first group was inseminated with vassal sperm in vitro alone (control); the seconed group (G2) was inseminated with vassal sperm in vitro using SMART medium enriched with low concentration of ATP(1.25mM). The third group (G3) was inseminated with vassal sperm in vitro using SMART medium enriched with high concentration of ATP (2.5mM). Mouse spermatozoa were collected from both vasdeferens. Then sperm parameters were assessed after 30 min. IVF technique was performed for 3 groups, and assessed after 20 - 22h. Then, percentages of early embryonic development stages and abnormal embryos morphology (%) for each embryo stage were assessed.Results : Results of the present study revealed significant increment (P<0.05) in the in - vitro fertilization (%) when using 1.25mM ATP as compared to 2.5 mM ATP and the control group. Significant differences (P<0.05) in the 1 - cell stage were assessed in 2.5mM compared with 1.25mM groups and the control group. Also, Significant differences (P<0.05) in the 2 - cell and 4 - cell stage were assessed in 1.25mM ATP compared with other groups. However, non significant differences in the percentages of abnormal embryos morphology were reported between both treated groups of ATP.Conclusion : From the results of present study it was concluded that the low ATP concentration (1.25mM) enriched to the culture medium improved the percentage of in vitro fertilization and early embryonic development.

العلاقة بين متلازمة تكيس المبايض وهرمون المضاد لمولر وبرامج تحريض الاباضة عند اجراء عملية التمنية داخل الرحم

Author name: عبير ناظم جاسم
Supervisor name: محمد باقر محمد رشاد فخر الدين | صباح مهدي حسين
General topic: Biology
Specific topic: Biotechnologies
Degree: Higher Diploma
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies
Language: English
University location: Baghdad
First pages:
Abstract: تعد متلازمة المبيض متعدد الا كياس هي من اكثر امراض الغدد الصماء شيوعا عند النساء حيث يصاب من النساء في سن الانجاب مايقارب (5 - 10%). الاعراض الرئيسيه لمرض متلازمة المبيض متعدد الاكياس هو اضطراب الدوره الشهريه مع فشل لاباضه وظهور اعراض زيادة الهرمون الذكر

نوعية البويضة والتكوين الجنيني بعد الاعطاء الفموي للسبيرماكس لاناث الفئران : موديل تجريبي للبائن == Oocyte Quality And Embryonic Development After Oral Administration of Speramax® In Female Mice : Experimental Model For Mammal

Author name: هبة صاحب حمزة
Supervisor name: سعد صالح الدجيلي
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
University: Al-Nahrain University - Higher Institute For Infertility Diagnosis And Assisted Reproductive Technologies
Language: English
University location: Baghdad
First pages:
Abstract: Background : Speramax® has been found to play an important role in sperm function characters and males reproductive performance with no studies on its effects on the oocyte maturation and embryonic development in females.Objective : The goal of the thesis is to examine the effect of Speramax ® on oocyte maturation. And to emphasize the effect of Speramax ® on ova quality, embryonic development and newborn. Materials and Methods : Speramax ® treated by oral administration for 1, 2 and 4 weeks. A hundred and twenty six female mice were randomly divided into four groups, the first group was treated without Speramax® with superovulation(SUO) while the second group were treated by Speramax® with SUO and the third group were superovulated only and the fourth group was not treated and spontaneously ovulated (SPO) and considered the control group(thirty two mice). Results : The results indicated that the treatment with Speramax® showed a positive effect on oocytes maturation in vivo. There was a highly significance (p? 0.0001) improvement in number of mature oocytes following treatment with Speramax® in SPO and SUO mice compared with SPO and SUO mice not treated with Speramax®. The embryonic developmental rate after 24 and 48 hours of mating in treated groups with Speramax® was significantly (p? 0.05) higher than those of SPO and SUO mice too.The study showed that the quantity and quality of embryos generated from the treated groups were superior to that of untreated groups.Conclusion : It was concluded that the treatment by Speramax® has a great improvement on oocyte maturation, early embryonic development and embryo grading quality of mice embryos with an increase in the numbers of mice newborn.