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العلاقة بين انتاج الانزيم المحلل للكولاجين وتكوين الغشاء الحياتي بوساطة بكتريا Pseudomonas aeruginosae == The Relationship Between Collagenase Production And Biofilm Formation By Pseudomonas Aeuroginosa

Author name: امال عزيز كريم السعدي
Supervisor name: شذى سلمان الطحان
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: A total of 359 samples divided as 228 clinical and 131 non clinical specimens were collected during 2012 from four hospitals in Baghdad city including : Al - Kadhymia Teaching hospital, Baghdad Teaching hospital, The Burn Specialist Hospital and Al - Imam Ali hospital, for isolation of P.aeruginosa to study the correlation between collagenase production and biofilm formation. Eighty two Pseudomonas isolates were screened for biofilm formation, 28 isolates were classified as strong biofilm formers, 25 as moderate and 27 as weak biofilm former. The 28 isolates were identifid by VITEk - 2 Compact system which confirmed that the isolates were P.aeruginosa. Collagenase production assay was used to screen 28 isolates that were strong biofilm formers inorder to detect the ability of these isolates to produce collagenase, the substrate of collagenase (collagen) was purified localy from bovin tendon and the results showed that just 8 isolates could grow in mineral salt media with collagen after 4 days of incubation. The factors affecting biofilm formation and collagenase production were studied to determine the optimual conditions for their production, those factors included : 1 - Nitrogen sources represented higher influence on collagenase production specialy (yeast extract) in media containing collagen than other media without collagen as a substrate. The specific activity differed between the 8 isolates, biofilm formation also became more pronounced with (yeast extract), while NH4Cl and NaNO3 depressed biofilm formation at the same conditions. The statistical analysis between the two parameters (biofilm and collagenase) according to different nitrogen sources demonstrated highly significance at p?0.01 with yeast extract and casein. 2 - pH, results showed that the best pH for production was 7 for both collagenase and biofilm.The statistical analysis for determination the relationshipe between the two parameters showed highly significance at p ?0.01 for different pH. 3 - The maximum production of the two parameters was at 35?C temperature which gave highly significance at p?0.01 with defferent temperature. 4 - Long incubation periods revealed increasing in collagenase production and biofilm formation which represented highly significance detween them when incubation periods were prolonged at p?0.01. Results of this study showed that collagenase production increases when bacteria switch from a planktonic to biofilm phenotype. This indicates that biofilms and collagenase are more virulent and have a greater ability to cause tissue destruction. The REP - PCR analysis using BOX - primer, showed a clusters genetic relatedness among the isolates. The isolates were grouped according to the REP - PCR in 9 different genotypes, named cluster 1 to 3 which included C1, C2, C3 with relatedness : 8 (80%), 8 (86%), 3 (80%) respectively. A19 and A20 both of them were not included in any cluster, they have 78% similarity.The REP - PCR analysis showed that the genotypic relatedness is consistently high between the 8 producer isolates and non producer isolates (13), showed similarity reached 86

الاصابة ببكتريا Helicobacter pylori وعلاقتها بمرض تصلب الشرايين القلبية == Correlation Between Helicobacter Pylori Infection And Atherosclerotic Heart Disease

Author name: رواء سعدي سلمان
Supervisor name: مي خليل اسماعيل
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: مرض قصور الشرايين التاجية هو النتيجة النهائية لتراكم اللويحات العصيدية ضمن جدران الشرايين التاجية مسببة نقص الاوكسجين وبالتالي حدوث مرض القلب الاقفاري. ويعتبر من الامراض الشائعة واحد الاسباب الرئيسية لحالات الوفاة في العالم. اضافة الى عوامل الخطورة التقلي | Coronary artery disease (CAD) is the end result of the accumulation of atheromatous plaque within the walls of the coronary arteries resulting in shortage of oxygen supply and ischemic heart disease (IHD). It was consider as one of the most common diseases and major causes of worldwide morbidity and mortality. In addition to traditional risk factors for cardiovascular disease, nowadays, accumulating evidence indicates that a variety of infectious agents may contribute to pathogenesis of CAD. The present study evaluated the anti - Helicobacter pylori IgG and IgA and the role of virulence factor of H. pylori cytotoxin associated gene (Cag A) and vacuolating associated cytotoxin (Vac A) as a risk factors for CAD.Eighty patients were divided into 2 groups : first group was 70 patients with CAD, the other group contained 10 patients with another coronary artery disease; they were admitted to Ibn Al - Bitar Specialist Center for Cardiac Surgery in Baghdad between October 2013 and January 2014. Ten individuals used as a healthy control group. All blood samples were tested biochemically such as glucose test, urea test, creatinine test and lipid profile test(cholesterol, triglyceride, LDL and HDL) and all of them showed normal results. The present study revealed that males were affected more than females (3 : 1); with no relation between their ages and bacterial infection.Serum IgG and IgA was Estimated by indirect immunofluorescent (IIF) whereas Cag A, Vac A and high sensitive C - reactive protein (hs - CRP) measured by enzyme linked immunosorbent assay (ELISA) and compared to control group results.The incidence of the anti - H.pylori IgG was highly significantly prevalent in CAD patients 78.57% (55/70), than in control group 0% (0/10), also a high significant difference in another CAD patient group 100%(10/10) as compared to control group 0% (0/10) (P? 0.01).Similarly anti - H. pylori IgA in CAD patients and another CAD patient groups showed highly significant increase when compared to control group 37.14% (26/70), 30% (3/10) and 0% (0/10) respectively (P? 0.01).The incidence of the CagA - positivity was significantly prevalent in mean value (2.74±0.19) in patients with CAD and another CAD patient group(2.72±0.31) than in control group (1.64± 0.16) (P? 0.05). Same result was observed with Vac A antigen; mean value of patient group showed significant increase (1.299 ± 0.04) when compared to controls group (1.41±0.13) (P? 0.05). Also significant increase found between another CAD patient group(1.79±0.17) and control group (1.41±0.13) (P? 0.05). Furthermore; the present study revealed significant differences of concentration levels between hs - CRP in CAD patient group (4.95 ± 0.38 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml), as well as a significant differences found between another CAD patient group (3.96 ± 0.96 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml) (P? 0.05).

التحري الجزيئي للجزر الوراثية المسؤولة عن المقاومة للمضادات الحياتية في العزلة baumannii A92 Acinetobacter == Molecular Detection Of Genomic Islands Responsible For Antimicrobial Resistance In Acinetobacter Baumannii Strain A92

Author name: سهاد سعد محمود العجيلي
Supervisor name: اليس كريكور ملكونيان | علي حسين ادحيه
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

القرابة الوراثية لجراثيم Acinetobacter baumannii المعزولة من مدينة الطب في بغداد == Genotyping Relatedness Of Acinetobacter Baumannii Isolated From Medical City - Baghdad

Author name: نظيمة حمود حسين السليم
Supervisor name: حارث جبار فهد المذخوري
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:

تاثير بعض مبيدات الادغال والحشرات في نمو ونشاط بكتريا العقد الجذرية في نبات الباقلاء Vicia faba == EFFECT OF SOME HERBICIDES AND INSECTICIDES ON GROWTH AND ACTIVITY OF ROOT NODULES BACTERIA ON Vicia faba PLANT

Author name: انمار سعدي عبود العكيدي
Supervisor name: نضال نعمة حسين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: Arabic
University location: Baghdad
First pages:

التلوث بالبكتريا الهوائية المرضية المعزولة من الجروح ما بعد العمليات والحروق والتعرف على بعض مصادرها وخاصة الكلبسيلا الرئوية في محافظة ذي قار == The Contamination by Aerobic Pathogenic Bacteria Isolated from Postoperative Wounds and Burns and Study some of Their Sources Especially Klebsiella pneumoniae in Thi-Qar province

Author name: خوام ريسان حسين الحسيني
Supervisor name: Myad F. Nakkash | Ilham s. Abdul Karim
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: English
University location: Baghdad
First pages:

التاثيــر البايولوجي لســموم بكتريا ذات الرئــة على الفئران البيضاء (داخل الجســم وخارجـــه ) == Biological Effects Of Klebsiella Pneumoniae Toxin In Albino Mice (In Vivo & In Vitro)

Author name: محمد سلمان محمد صريح
Supervisor name: عصام فاضـل الجميلــي | ناهــي يوســف ياســين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
University: University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language: English
University location: Baghdad
First pages:

التحري عن وجود جرثومة Staphylococcus Saprophyticus في حالات اصابات الجهاز البولي في الانسان وبعض الحيوانات == Investigation about STAPHYLOCOCCUS SAPROPHYTICCUS in urinary tract infection in human and some animals

Author name: عبد الكريم سلمان صكبان اليساري
Supervisor name: حسن علي عبد الرضا | محمد صبري عبد الرزاق
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine - Department Of Microbiology
Language: Arabic
University location: Baghdad
First pages:

دراسة جرثومية مصلية عن اهم مسببات الاجهاض في النساء مع التركيز على داء البروسيللوسيز == Bacteriological and Serological Study of Important Causes of Abortion in Women with Concentrated on Brucellosis

Author name: لمى حكمت كريم البياتي
Supervisor name: غازي موسى الخطيب
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine - Department Of Microbiology
Language: Arabic
University location: Baghdad
First pages:

الدور الوقائي المتعدد السكريد الشحمي البكتريا seudomonas aeruginost ضد خمج قرنية العين

Author name: جنان محمد حسن العزاوي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

تاثير التلوث البكتيري لنهر ديالى في بيئة نهر دجلة

Author name: ايثار كامل عباس الميالي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

دراسة تاثير بعض العوامل البيئية والمواد الكيميائية الحافظة في نمو البكتريا Escherichia الممرضة COLI

Author name: علياء رزوقي حسين
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

الاحوال (الظروف) المناسبة للحفاظ على الفاسشيولا جاجينكا في الاوساط الخالية من البكتريا

Author name: نهلة حنا العفاص
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad
University location: Baghdad

خواص انزيم الفوسفتيز القاعدي للمكورات العنقودية الذهبية المسببة لالتهاب الضرع في الابقار العراقية

Author name: احلام عبد الرزاق محمد
Supervisor name: فاروق خالد حسن
General topic: Veterinary Medicine
Specific topic: Microbiology - Bacteria
Degree: Master
University: University of Baghdad - College Of Veterinary Medicine - Department Of Microbiology
Language: Arabic
University location: Baghdad
First pages:
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