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Pathogenesis Of Three Newcastle Disease Virus Strains

اسم المؤلف: سنا عبد السلام عبد الوهاب
اسم المشرف: الفلوجي
الموضوع العام: الطب البيطري
السنة: 1978
الموضوع الدقيق: الامراض
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

studies on mastitis in buffaloes

اسم المؤلف: abdul wahab razak
اسم المشرف: s. kalra
الموضوع العام: الطب البيطري
السنة: 1982
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

عزل وتوصيف جرثومة السل من الابقار في مدينة بغداد وضواحيها == isolation And Identification Of Mycobacteria From Cattle In And Around Baghdad

اسم المؤلف: جون داود قسطندي
اسم المشرف: سعدي احمد غناوي | D.P.N.Kamalapur
الموضوع العام: الطب البيطري
السنة: 1984
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد

Studies On Mastitis In Goats

اسم المؤلف: maulood abbas ali al - graibawi
اسم المشرف: v.k. sharme
الموضوع العام: الطب البيطري
السنة: 1983
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد

دراسات مرضية حول الاغنام المخمجة تجريبيا بحمة البارا انفلونزا PI_3Virus_3 == Pathological Studies on Sheep Experimentally Infected With Parainfluenza Type-3 Virus

اسم المؤلف: محمود مهدي محمود
اسم المشرف: Talib A. Makkawi
الموضوع العام: الطب البيطري
السنة: 1986
الموضوع الدقيق: الامراض
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

داء البريميات : دراسة سيرولوجية في الابقار والاغنام والماعز في العراق == Leptospirosis Serological Study In Cattle Sheep And Goats In Baghdad

اسم المؤلف: طه ياسين غني البدراوي
اسم المشرف: P.N.Kamalapur
الموضوع العام: الطب البيطري
السنة: 1984
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد

عزل وتجنيس فايروس مرض نيوكاسل محليا من قطعان الدواجن المصابة طبيعيا في شمال العراق == ISOLATION AND GENOTYPING OF LOCAL NEWCASTLE DISEASE VIRUS ISOLATED FROM NATURALLY INFECTED CHICKENS IN NORTHERN IRA

اسم المؤلف: احمد ابراهيم احمد
اسم المشرف: شوني ميخائيل اوديشو | رباح نجاح جبار
الموضوع العام: الطب البيطري
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Newcastle disease (ND) has been concerned as one of the most important devastating diseases of poultry because of its wide range host of birds and worldwide distribution with severe economic losses in domestic poultry, due to the severe nature of the disease, ND is included as a list A disease.
Iraq has been endemic for Newcastle disease virus (NDV) with natural infection causing significant losses in the poultry industry since 1968 by the NDV/AG68 strain (AF001108) from infected chickens at Abu Graib in Iraq.
Currently, NDV genotype VII are most frequently associated with outbreaks of ND in the Middle East and Asia which have showed higher mortality in vaccinated poultry. The main objective of this study was to determine the biological and molecular characterization of the pathogenicity and genotyping followed by phylogenetic analysis. To evaluating the degree of genetic diversity of local isolated NDV strains and estimating the relationships to that NDV presence in the neighboring region, as well as preparation of inactivated vaccine against NDV from local strains was determine.
At the beginning, this study was carried out to isolate Newcastle disease virus (NDV) in naturally infected chickens of poultry farms in the north of Iraq. The study initiated diagnosis of 46 farms out of 96 broiler farms exhibited Newcastle disease (ND) (26 in Erbil, 14 in Sulaymanhia and 6 in Duhok) based on clinical signs corresponding with (8-15%) mortality and gross lesions of scarified chickens. The rapid test for detection NDV antigen from infected chickens for tracheal swab indicated that 32 farm samples were positive out of 46 initiative diagnosed farms.
Based on intiative diagnosis the identification of local isolates of NDV samples was pooled lung with trachea, spleen, cecal tonsil and intestine, were pooled in nine samples, then tissue homogenates were made from all samples in sterile Phosphate Buffered Saline (PBS) containing antibiotics, after centrifugation of tissue homogenates the supernatant fluids were assessed to real time reverse transcriptase- Polymerase Chain Reaction (RRT-PCR) assay. The results indicated that six
homogenate samples were positive for NDV and negative for Avian Influenza Virus.
After that, the remain supernatant fluids were used for virus isolation in embryonated chicken eggs then harvested allantoic fluids were used for employed for the hemagglutination activity. Six samples were showed positive results. Furthermore, virus identification was also performed by using conventional diagnostic methods such as Hemagglutination(HA) of red blood cells followed by Hemagglutination Inhibition (HI) test applying the prepared hyper immune serum(HIS) against NDV antigens to identify the NDV. The serological results demonstrated that six samples of NDV were positive, as well as the (rt-PCR) assay was performed for allantoic fluid to insure the presence of NDV using specific primers and probes to amplify specific region in the M gene of the viruses. The results revealed that six isolates were positive for NDV.
In addition, the pathogenicity of the six isolates was performed according to standard methods including Mean death times (MDT), Intra-cerebral pathogenicity index (ICPI) and Intra-venous pathogenicity index(IVPI), which revealed that six isolates were compatible to mesogenic (1/6) and velogenic (5/6) types.
At the second step, the molecular characterization of six isolates were investigated in Borehingher laboratory (Germany). The RT-PCR successfully amplified a 436 bp DNA fragment covering a part of the matrix (M) gene, fusion (F) gene (lentogenic) and fusion (F) gene (subgenotype VIIi) from all six isolates (NDV). The result was indicated that 5/6 were positive for velogenic NDV strain of genotype VIIi and 1/6 were negative. The nucleotide sequences of F gene were also determined, where the portion of the F2 gene of five isolates compared with that of other NDV strains retrieved from GenBank. Phylogenetic analysis showed that the isolates NDV belong to genotypes VIIi.
However, in local laboratory the result of FAST one-step quantitative Polymerase Chain Reaction (qPCR) successfully amplified a 525 bp fragment covering a part of the fusion (F1) gene and 515 bp fragment covering a part of the fusion (F2) gene from all six isolates. The nucleotide sequences of the PCR product of E1, S1 and S2
were found in F1 gene, while E1, E2, S1, S2, and D2 found in F2 gene out of six NDV isolates. Those results were compared with other NDV strains which retrieved from GenBank. Phylogenetic analysis showed that the Iraqi isolates are belonging to genotypes VII.
Finally, Inactivated vaccine was prepared locally using Velogenic NDV genotype VIIi isolate as a seed virus ELD50/0.1ml109 then compared with a commercial inactivated vaccine in an experimental study. This study was conducted by purchasing 120 broiler chicks and divided into three equal groups. Group 1 as unvaccinated control group , G2 for commercial vaccine and G3 for local vaccine), G2 and G3 were injected s/c with inactivated vaccine at 3 days old followed by a booster dose with LaSota live vaccine eye drop. Indirect ELISA technique was used to measure of antibody titer at ages 7, 17 and 27 days old (pre-challenge), the result showed at 7 days-old were (4251±1078) for all groups, and at 17 days-old were (906±33, 844±153 and 1145±219) for G1, G2 and G3 respectively. There were significant differences (p
الملخص:
المصادر:

الكشف عن داء الكلاميديا في الاغنام والماعز بواسطة اختباري الاليزا وتفاعل البلمرة المتسلسل == Detection of Ovine and Caprine Chlamydiosis by ELISA and rtPCR

اسم المؤلف: عمر عطا الله فهد
اسم المشرف: سفيان صالح سلمان
الموضوع العام: الطب البيطري
السنة: 2017
الموضوع الدقيق: الطب الباطني والوقائي
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

التحري عن بعض عوامل الضراوة للزائفة الزنجارية المعزولة من الحليب الخام والجبن الطري == Detection of Some Virulence Factors of Pseudomonas Aeruginosa Isolated From Raw Milk And Soft Cheese

اسم المؤلف: عبد الكریم كاظم عبد الحسین الحسب
اسم المشرف: علي حسن احمد الشمري
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: صحة الغذاء
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: In order to detection of Pseudomonas aeruginosa in food - chain especially from Cows raw milk and soft cheese with its whey, this study was designed in some regions of Baghdad (College of Veterinary Medicine, Abu - Ghraib, Al - Sadrya & Al - Radwaniyah), in which a standard isolation methods were used with some modification processing by new, modern and rapid technology tools such as chromogenic medium CNP agar and Electronic rapid Microbact TM 24E panel identification system supported by standard color differential chart and online American Type Culture Collection (ATCC) Codes; aids in rapid and precise identification, differentiation, confirmation and enumeration of P. aeruginosa isolates from raw dairy samples; as well as, detection of biofilm producing versatile morphotypes with chameleon phenomenon and fruity odour; and demonstration the activity of food spoilage enzymes (protease, lipase & lecithinase) of isolates with thermo - stability and viability in different temperatures with bitty cream and ropy fermentation phenomenon. The results profile showed : 1. Isolation of 46 (76.7%) isolate of P. aeruginosa from a total of 60 Cow Dairy samples : 30 Raw Milk samples (pooled from milk cans and apparently healthy Cows with a history of mastitis cases) as 19 (31.7%) isolates from regions of College of Veterinary Medicine, Abu - Ghraib and Al - Sadrya (10 from each region); and 30 Soft Cheese with its Whey samples (pooled from unhygienic processed cheese and containers in unclean dirty environment and apparently carrier or diseased workers) as 27 (45%) isolates from regions of Abu - Ghraib, Al - Sadrya and Al - Radwaniyah (10 from each region). Isolation percentages of oxidase positive P. aeruginosa from selected Regions in Baghdad Province indicates significant differences among regions at a level (P?0.05), in which highest isolation percent of P. aeruginosa from Abu - Ghraib as 20 (33.4%) isolates (10 isolates from raw milk and 10 isolates from soft cheese) in accordance to 17 (28.4%) isolates from Al - Sadrya (9 isolates from raw milk and 8 isolates from soft cheese), 9 (15%)SUMMARYisolates from Al - Radwaniyah (9 isolates from soft cheese) without any isolate from College of Veterinary Medicine, which may indicate good hygienic measurements.2. Segregation of isolates into two Haemolytic Patterns : Livestock type origin, lysis only sheep blood (L - type) as 19 (41.30%) isolates from raw milk samples; and Zoonotic type lysis, both blood (Z - type) as 27 (58.70%) isolates from soft cheese with its whey as high prevalence due to critical processing chain, risky carrier individuals and contaminated environment or polluted water supply. This may indicate indirectly genetic diversity and host or cross individual tropism in haemolysins - phospholipases activity according to different types of samples, regions and hosts.3. The study revealed isolation and segregation of versatile morphotypes and five pigments with fruity odor (Chameleon phenomenon) with mucoid drippy due to alginate or small colonial variants smooth or rough or both especially in isolate code M7 from mastitic milk and isolate code C27 from contaminated soft cheese and its whey. Wrinkled - Corroded green morphotypes were more evident in blood agar due to secretion of siderophore pyoverdine in order to chelating iron for activation of isolate. Motility Pattern with TTC salts was evident in all isolates with three types of motility : swarming, swimming and twitching, that noticed obviously in M7 isolate.4. New technology biochemical identification panel system (Microbact TM 24E) confirm that isolates were P. aeruginosa at level (P?0.01) after online matching with standard Remel ATCC Octalcodes bank of P. aeruginosa, in which 5 octalcodes typical and atypical were documented, that indicate genetic diversity in biochemical reactions of isolates and their morphotypes, modified microbial gene sharing protocol of P. aeruginosa with other bacteria and highly Sensitivity and Specificity of the test panel.SUMMARY5. Detection and isolation of Biofilm producing isolates by five methods, in which microtiter plate assay was the most sensitive and realizable technique while Congo red agar test detect quantity and type of biofilm production through colour of colonies (black very strong and huge, gray strong, pink moderate and white fair or non - producer). Biofilm produced in most morphotypes with pellicles at liquid interphase and ropy viscous threads (sliminess) and bitty cream especially in isolate codes M7 and C27. Small colonial variants of some morphotypes can revert to mucoid drippy variants. M7 showed unusual ropy thread of 30 cm long.6. The results showed secretion of thermo and psychro stable and labile food spoilage enzymes (protease, lipase and lecithinase) from isolates, in which versatile morphotypes showed indirectly diverse genetic ability, this may indicate variation of thermal tolerance mechanisms (heat shock proteins and biofilms formation) in isolates in accordance to time and temperature of processing, that linked genetically with the quorum sense mechanisms of acyl homoserine lactone gene regulatory region (AHL) in P. aeruginosa isolates (Stress Hardening phenomenon).7. The results showed the genetic ability (indirectly) of some isolates to resist, tolerate and proliferate in acidic environment at pH 2 & 4 especially isolate M7 & C27, while intermediate to susceptible tolerance of pH were noticed in others. This may indicate the power of electromagnetic charged net field of complex polymeric matrix of alginate exopolysaccharide layers in protecting some isolates from adverse acidic environment due to quorum sensing behavior of M7 & C27.Therefore, it could be concluded from this study that P. aeruginosa was prevalent in food chain especially from Cows raw milk and soft cheese with its whey samples from some regions in Baghdad, and Biofilm producing isolates with their thermo and psychro stable enzymes complex system and their tolerance to acidic environment stressors were more dangerous thus, we recommend monitoring these products periodically for insurance of public health.

مقارنة فعالية المضادات البكتيرية لثلاث ادوية من مناشئ مختلفة في خارج وداخل الجسم الحي == Comparison Of Antibacterial Activity Of Three Different Drugs Obtained From Different Origins In Vitro And In Vivo

اسم المؤلف: ياسر محمد محي
اسم المشرف: علي عزيز الخياط
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الفسلجة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

دراسة مقارنة لثلاثة انظمه للتخدير العام في الماعز == Comparative Study Of Three Protocols Of General Anesthesia In Goats

اسم المؤلف: عمر خالد عزيز
اسم المشرف: رفل عبد الدائم
الموضوع العام: الطب البيطري
السنة: 2014
الموضوع الدقيق: الجراحة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

دور مستويات مختلفة من الانزيمات المحللة للالياف Fibrolytic Enzymes على اداء الحملان الذكرية العواسية == The Role Of Different Levels Of Fibrolytic Enzymes On The Performance Of Awassi Ram Lambs

اسم المؤلف: علي مهدي محمود ماني
اسم المشرف: مظفر نافع رحو الصائغ | ضياء عبد الامير الخياط
الموضوع العام: الطب البيطري
السنة: 2015
الموضوع الدقيق: الصحة العامة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

داء السالمونيلا في الانسان والحيوان == Salmonellosis In Human And Animal

اسم المؤلف: علاء محسن حمودي الجصاني
اسم المشرف: زينب رزاق زغير
الموضوع العام: الطب البيطري
السنة: 2015
الموضوع الدقيق: الامراض المشتركة
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

تقييم المراحل المختلفة للحمل في النعاج بواسطة استخدام الامواج فوت الصوتية == Evaluation Of Different Stages Of Pregnancy In Ewe By Ultrasonography

اسم المؤلف: سهام سلمان احمد المشهداني
اسم المشرف: طالب موسى عبد الله الحميداوي
الموضوع العام: الطب البيطري
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

Postnatal histomorphological development of the ovary, uterine tube and uterus in normal and ovariectomized local rabbits (Oryctologus cuniculus)

اسم المؤلف: منى رضا علي الدهان
اسم المشرف: فرحان عودة ربيع | احسان حمودي التميمي
الموضوع العام: الطب البيطري
السنة: 2015
الموضوع الدقيق: التشريح والانسجة والاجنة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

دراسة تشريحيـــة ونسيجيــة للغدة الدمعيـــة وغدة الرف فــي الماعــــــــز الاســــــود Caprus hircus == Anatonical And Histological Study Of Lacrimal And Nictitans Glands In Black Goats Caprus Hircus

اسم المؤلف: معزز عبد الخالق حيدر الرماحي
اسم المشرف: فرحان عودة ربيع
الموضوع العام: الطب البيطري
السنة: 2008
الموضوع الدقيق: التشريح والانسجة والاجنة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى: