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كشف النمط الوراثي وتقدير تكرار اليلات مجموعة الدم ABO وعامل Rh في مجتمعات محافظة صلاح الدين وكركوك ونينوى

Author name: رشا جمال جمال الدين مصطفى النعيمي

Supervisor name: عادل فوزي شهاب

General topic: Biology

Specific topic: zoology - Genetics

Degree: Doctorate

University: Tikrit University - College Of Education For Pure Sciences - Department Of Biology

University location: Salahaddin

دراسة فسلجية ووراثية جزيئية لعينة من مرضى السمنة فـي العـراق

Author name: مصطفى نهاد جمعة الدراجي

Supervisor name: ناهي يوسف ياسين

General topic: Biology

Specific topic: zoology - Genetics

Degree: Doctorate

University: Tikrit University - College Of Education For Pure Sciences - Department Of Biology

University location: Salahaddin

دراسة تاثير مستخلصات الشاي الاخضر في ازالة السمية الوراثية للافلاتوكسين في الفئران البيضMusmusculus

Author name: سيف حسان علي

Supervisor name: كركز محمد ثلج الجبوري | عادل فوزي شهاب

General topic: Biology

Specific topic: zoology - Genetics

Degree: Master

University: Tikrit University - College Of Science - Department Of Biology

University location: Salahaddin

دراسة السمية الوراثية لثنائي اوكسيد الكبريت(SO2) والامونيا (NH3) في الخلايا السرطانية والطبيعية في المختبر وفي داخل الجسم الحي باستخدام تقنيات مختلفة

Author name: وجدي صبيح صادق

Supervisor name: عادل فوزي شهاب | ناهي يوسف ياسين

General topic: Biology

Specific topic: zoology - Genetics

Degree: Doctorate

University: Tikrit University - College Of Education For Pure Sciences - Department Of Biology

University location: Salahaddin

تاثير التعدد الشكلي HindIII وSer447Ter لجين اللايبوبروتين لايبيز على ايض اللبيدات وتركيزها لدى مرضى التصلب العصيدي في مدينتي تكريت وسامراء

Author name: عمر رحيم خلف

Supervisor name: عادل فوزي شهاب

General topic: Biology

Specific topic: zoology - Genetics

Degree: Master

University: Tikrit University - College Of Education For Pure Sciences - Department Of Biology

University location: Salahaddin

دراسة وراثية خلوية وجزيئية للتاثير الوقائي للكركم في سمية العقار ميترونيدازول في الفئران البيض == Cytogenetic And Molecular Study of Protective Effect of Curcumin In Toxicity of Metronidazole In White Mice

Author name: فائق ابراهيم علي محمود

Supervisor name: عادل فوزي شهاب

General topic: Biology

Specific topic: zoology - Genetics

Degree: Doctorate

University: Tikrit University - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: صممت الدراسة الحالية لغرض التعرف على التاثيرات السمية الوراثية والسمية الخلوية للعقار ميترونيدازول(MTZ) في الفئران البيض Balb/ C والتاثير الوقائي الذي توفره المعاملة المسبقة بالكركم(CUR) في سمية العقار ميترونيدازول. تم استخدام مؤشرات الوراثة الخلوية (النو | The current study was designed to identify the genotoxicity and cytotoxicity of Mitronidazole (MTZ) and protective effect of Curcumin (CUR) against the toxicity of MTZ in whit mice. Cytogenetic endpoints (micronuclei (MN) and mitotic chromosomes), Productive biomarkers (sperms), molecular cytogenetic biomarkers (comet assay), molecular endpoints (genomic and mitochondrial DNA concentration in liver and spleen and testis) and mutation assessment in mitochondrial DNA (mtDNA) of liver and testis by PCR and restriction pattern were used to evaluate genotoxicity, cytotoxicity of MTZ and protective effects of CUR in white Balb/Cmice. The study was conducted in the laboratories of college of education, college of science in Tikrit university and laboratories in the state company for drugs industry - Samarra - Iraq (SDI). Dosages of 30 and 50 mg/kb.b.wt. of MTZ were tested for genotoxic and cytotoxic and mutagenic effects in somatic and sperms of white mice. The dosages 4.5 and 10 mg/kgb.wt. of CUR were used to study the protective effects of CUR in genotoxicity and cytotoxicity and mutagenecity of MTZ. The current study showed that MTZ has genotoxic effect expressed by high frequency of micronuclei in polychromatic erythrocytes (PECs) which reached 15.5 ± 2.88 with the dosage 30 mg/kg.b.wt. and 21.31 ± 4.2 with the dosage 50mg/kg.b.wt. compared with 7.25 ± 1.88 in negative control. In the study of the protective effects of CUR against genotoxic and cytotoxic effects of MTZ, we observed that in mice treated with the dosage 4.5 mg/kg.b.wt of CUR for 9 days before the dosage 50 mg/kg.b.wt. of MTZ, There were significant p<0.05 decrease in MN frequency which reached 13.25 ± 4.19 compared with 21.31 ± 4.2 for mice treated with the dosage 50mg/kg.b.wt. of MTZ withoutCurcumin. The study showed that MTZ has cytotoxicity expressed by significant p<0.05 decrease in mitotic index (MI) which reached 0.010 in bone - marrow of the mice treated with 50 mg/kg.b.wt. compared with 0.023 in negative control. The dosage 4.5 mg/kg.b.wt. of CUR for 9 days before giving the dosage 50 mg/kg.b.wt. of MTZ resulted in returning of MI in bone - marrow 0.021 to its levels in negative control 0.023 compared with its value which reached 0.010 with the dosage 50 mg/kg.b.wt. of MTZ. The two dosages 30 and 50 mg/kg.b.wt. of MTZ caused significant p<0.05 decrease in total number of sperms which reached 2.4±2.4 and 1.5 ± 0.1 milions simultaneously compared with 6.7 ± 0.9 millions in negative control. More over there were significant p<0.05 decrease in motile sperms which reached 25.2 ± 11.0 and 14.6 ± 10.6 compared with 57.6 ± 8.0 of negative control. Significant p<0.01 decrease in the length of mid - piece and total length of sperm tail with the dosage 50 mg/kg.b.wt. of MTZ which reached 19.2 ± 6.0 and 69.0 ± 6.0 simultaneously compared with 31.6 ± 9.0 and 100.3 ± 15.0for negative control. Treating with the dosage 4.5 mg/kg.b.wt. of CUR for 9 day before the dosage 50 mg/kg.b.wt. of MTZ resulted in the return of mid - piece and total length of sperm tail which reached 29.5 ± 3.0 and 95.0 ± 5.0 close to the levels of negative control 31.06 ± 9.0 and 100.3 ± 15.0 simultaneously. Compared to 19.2 ± 6.0 for the length of mid - piece and 69.0 ± 6.0 for total length of sperm tail in mice treated with the dosage 50 mg/kg.b.wt. of MTZ without previous treatment with CUR. The study of damage levels in DNA with comet assay showed that MTZ induced significant p<0.01 levels of damage reached 45.4 ± 2.36 with the dosage 50mg/kg.b.wt. of MTZ in bone - marrow and 36.8 ± 0.58 in liver cells compared with 9.0 ± 2.12 and 5.2 ± 0.58 simultaneously in negative control. There were decrease occurred in the level of damage in DNA of bone - marrow and liver cells. However it did not return to its levels of negative control 9.0 ± 2.12 in bone - marrow and 5.2 ± 0.58 in liver cells. OTM values reached 26.4 ± 1.29 and 24.0 ± 1.14 in mice treated with the dosage 4.5 mg/kg.b.wt. of CUR for 9 days before the dosage 50 mg/kg.b.wt. of MTZ. However The values of OTM lower than its values in bone - marrow 45.4 ± 2.36 and 36.8 ± 1.66 in liver cells of mice treated with the dosage 50 mg/kg.b.wt. without CUR. In the molecular study, The results showed mutagenic effect of the dosage 50 mg/kg.b.wt. of MTZ in mice mtDNA expressed by novel restriction sites for Bam - HI and Hind - III in liver tissue and SauA3 in testis. The treatment of mice with the dosage 4.5mg/kg.b.wt. of CUR for 9 days before the dosage 50mg/kg.b.wt. of MTZ did not resulted in the inhibition of mutagenesis in mtDNA. In conclusion, results of this study showed that MTZ has genotoxic and cytotoxic effects in white mice and mutagenic effect in mice mtDNA. The dosage 4.5 mg/kg.b.wt.of Curcumin has protective effect against genotoxic and cytotoxic effects of MTZ. on the other hand the dosage 4.5 mg/kg.b.wt. of Curcumin has no protective effect against mutagenicity of MTZ in mice mtDNA.

دراسة بعض المؤشرات الوراثية الخلوية لتاثير مادة الكلوروفورم على الفار الابيض Mus musculus L == A Study On Some Cytogenetic Indices For The Effect of Chloroform On The White Mouse Mus Musculus L

Author name: عمر رحيم خلف

Supervisor name: عادل فوزي شهاب

General topic: Biology

Specific topic: zoology - Genetics

Degree: Master

University: Tikrit University - College Of Science - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

Abstract: يستخدم الكلوروفورم على نطاق واسع في العديد من الصناعات مثل صناعة الورق والمطاط ومطافئ الحريق ومستحضرات التجميل ومواد التخدير. كما يستخدم كمذيب عضوي للعديد من المواد في المختبرات التعليمية والصناعية. وينتج الكلوروفورم من العمليات الايضية التي تقوم بها بعض | Chloroform is widely used in many industries, such as paper, elastic, fire extinguisher, cosmetics and anesthetic materials industries. It also used as an organic dissolvent for many materials in industrial and education laboratories. Chloroform is produced by The metabolism process of living organisms such as macroalgae and some fungus of highly organic matter soil. It can also be produced by process of water cholornation. Because of chloroform is widely used, and few studies achieved on cytogenetic level. Therefore The present study was conducted to identify The effects of chloroform on cytogenetic characteritis in laboratory mice.This study includes : 1 - A Study on Some Cytogenetic Indices For The Effect of Chloroform on The White Mouse.2 - Sperm abnormalities of male mice.The results of The present study showed that The chloroform has the ability to motivate chromosomal aberrations which were represented by (ring chromosom, chromatid break, and centromore break) in somatic cells in both males and females mice and in germ cells in males, The results revealed significant differences in The chromatid break and ring chromosome of both male and females mice, which injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with control group. It was found That there were asignificant differences in centromore break of females, injected with two concentrations of chloroform in comparison with control group, while non significant were differences observed in The males.Also the present study showed there were significant differences in The chromosomal aberration in The germ cells of males mice, injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with controlled group.The concentration (0, 05 mg/kg) was more influent in comparison, with The concentration (0, 025mg/kg). It was found that 30 days of injection is more influent in comparison with 15 day of injection, and males are more influenced than females.The present study recorded a significant differences in creating micronuclei in both male and female mice, injected by chloroform with a concentrations (0, 025 & 0, 05 mg/kg)in compared with control group.The result also revealed that The chloroform cause a significant decrease in The mitotic index of The somatic cells in both males and females, morever significant decrease in The mitotic germ cells of The injected males mice was found.An increase in The Blast index factor in The somatic cell in both male and female mice, injected by chloroform when compared with control group was similar result was recoded rgarding The Blast Index factor of The males germ cells. Regarding The sperm abnormality, the result showed significant differences in The sperm abnormality of males injected by chloroform with two concentrations (0, 025 & 0, 05 mg/kg) in comparison with The control group. The concentration (0, 05 mg/kg) was more influent than The concentration (0, 025mg/kg). Besides and the period 30 days of injection was more influent than the period 15 days.

دراسة وراثية خلوية وجزيئية لعقم الرجال في محافظة الانبار == Cytogenetic and Molecular study of Male Infertility in Al-Anbar Governorate

Author name: سمير مشرف خلف الدليمي

Supervisor name: عادل فوزي شهاب | عقيل حسين علي

General topic: Biology

Specific topic: zoology - Genetics

Degree: Doctorate

University: Tikrit University - College Of Science - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

عزل وتشخيص بكتريا القولون E.coli من اصابات الجروح والحروق ودراسة بعض عوامل ضراوتها باستخدام تقنية ال PCR == Isolation and identificate of E.coli isolated from infections wounds , burns and study of some verulance factors using the PCR tichnique

Author name: بثينة جاسم يوسف

Supervisor name: فواز عبد الوهاب الدباغ | وعد محمود رؤوف

General topic: Biology

Specific topic: zoology - Genetics

Degree: Doctorate

University: Tikrit University - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages:

دراسة تشخيصية ووراثية للبكتيريا المسببة لالتهابات المجاري البولية للمرضى في مدينة تكريت == Diagnostic and Genetic Study of the Bacteria Causing Urinary Tract Infections in Patients in Tikrit City

Author name: انس ياسين محمود الدوري

Supervisor name: خالد دحام احمد | عادل فوزي شهاب

General topic: Biology

Specific topic: zoology - Genetics

Degree: Doctorate

University: Tikrit University - Faculty Of Education - Department Of Biology

Language: Arabic

University location: Salahaddin

First pages: