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دراسة التشوهات الكروموسومية والخلوية للمبيدين الكلايفوسيت واللامبدا سيهالوثرين في الخلايا الحيوانية والنباتية == A study of TheChromosomal Aberrationand Cytotoxicity forGlyphosate and Lambda cyhalothrinin Animal and plant cells

Author name: زﯾﻨﺐ ﺟﻠﯿﻞ ﺧﺰﻋﻞ
Supervisor name: فائزة عبد الوھﺎب احمد
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:

تنسيل وتعبير جين السوماتوتروبين من الابقار المحليه في البصره == Cloning and Expression of Somatotropin Gene from Local Cows in Basrah

Author name: رشا منذر عثمان
Supervisor name: محمد عبد المحسن معراج الحجاج
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: English
University location: Basrah
First pages:

انتاج بروتين cry1I من بكتريا Bacillus thuringiensis بواسطة التنسل الجيني == Production of cry1I Protein from Bacillus thuringiensis by Gene Cloning

Author name: نسمة طالب وناس علي
Supervisor name: محمد الحجاج
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Master
University: University Of Basrah - College Of Science
Language: Arabic
University location: Basrah
First pages:
Abstract: Bacillus thuringiensis (commonly known Bt) is a ubiquitous, gram - positive and spore - forming bacterium. The organism produces intracellular crystal proteins, which are toxic to insects, during the stationary phase of its growth cycle. Because of its insecticidal activity, B. thuringiensis has been used as a biopesticide. However, it is still necessary to search for more toxins to control insect orders, which have the ability to develop resistance against such pesticides, and also to provide alternatives for chemical insecticides. The purpose of this study is to isolate B. thuringiensis strains that are collected from Basrah, and to identify the cry genes content of these isolations.In this study, 344 Bacillus species were isolated from 22 soil samples collected from different area from Basrah, fifty six of them (16,2%) were identified as B. thuringiensis strains based on colony morphology , microscopic observation of spore position in the cell and genetic analysis . Most isolations were examined by multiplex PCR using for cry 1, cry 2, and cry 9 universal primers in order to identify the type of cry gene content of these isolations. 82% of the isolations amplified cry1 gene, 76% amplified cry9 and 21% amplified cry2 genes.In this study cloned cry1I gene is using specific primer to amplify full length of gene. The cry 1I gene (2169 bp) amplified product was inserted in to the Pst1 and BamH1 sites of pdrive cloning vector joining technique to produce the recombinant vector. The cloning vector then transformed in to E.coli HB101 and the transformant cells colonies were selected by ampicillin sensitive phenotype, the efficiency of transformation was also determined to be 7,8 ×105cfu/μg. After that the cry1I protein is purified from LB broth media supported with ampicillinand used this protein against Tuta absoluta larva within concentrations (100 μg/ml, 150μg/ml, 200μg/ml) .The effectiveness of the toxin is to kill the larvae were in the concentration of (200μg/ml) in the 24 hours after treatment, while least toxicity in the concentration (100μg/ml) need three days to kill all larva.

دراسة جزيئية لتعدد الاشكال الوراثية لجين TAS2R38 (جين تذوق مادة PTC) بين سكان محافظة البصرة وعلاقتها بمرض السكري == A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes Mellitus

Author name: حسنة عامر مهوس
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study dealt with the genetic polymorphism of Phenylthiocarbamide (PTC) tasting from phenotypic and genotypic (Molecular of TAS2R38(P49A) gene ) prospective,among sample of Basrah Population from 2014 - 2012. Also, a comparative Molecular study for the gene TAS2R38(P49A) and some biochemical parameters was made in a group of Diabetic patients for the first time in Iraq /Basrah and the world .Phenotypic study using the gradient dilutions of PTC substance method, was established among 317 individual : 96Males and 221 Females from different regions of Basrah. Increased sensitivity with low threshold 6 of Males than Females 5 was observed which indicate that the Males were more sensitive than Females in this region from Iraq .However, the tasters percent among Females still higher73.3% than Males72.9% ,and the whole population 73.2% as well .This study recorded different tasters percent in different regions : 62.1%,71.8% ,60.6%, 82.3% for the North ,AL - Zubair and Safwan, Abu - ALChaseab and AL - Fao and the center of the city respectively, although ,the whole population of Basrah found to be under Hardy - Weinberg Equilibrium . Allelic frequencies according to phenotypic analysis showed : recessive allele t had higher frequency (0.53) than dominant allele T (0.47), with elevated Heterozygous frequency Tt among all studied regions and the whole population(0.53) .The results recorded also , high BMI for non tasters tt as a whole and for both sexes in this phenotype : 28.32 ,28.26 for males and females respectively. While it had the lowest value among heterozygous 23.40 ,Statistical analysis for phenotypic study of this locus reveald an additive effect of the two alleles T,t by 81.33% with responsibility of 83.10% for the phenotypic variation found in this trait . Genetic (Molecular) analysis was done for comparative individuals and diabetic patients by sequencing the hTAS2R38(P49A) the PTC gene, among 127 individual : 59 healthy individual as a comparative group and 68 diabetic patients from both sexes and same age ranges. The results recorded a C/G allele in the position of the first SNP (145 C/G) which encode for Proline amino acid (C) or Alanine amino acid (G) : CC , CG refer to Tasters genotypes while GG refer to Non - tasters genotype. High frequency of C allele as compare to G among both healthy people and diabetic patients : 0.55, 0.53 for both respectively . Frequency of heterozygous genotype CG recorded a significant access as 0.76 among the comparative group which may implies a selective heterozygosity advantage among Iraqi population at this region. A significant differences in genotypic distributions was recorded between comparative and diabetic groups with high incidence of non tasters phenotypically tt 41% and genotypically GG 20.5 % among diabetic patients compare to healthy people : 25 % , 7% respectively . Interestingly non taster GG genotype had the lowest BMI among diabetic patients compare to othertwo taster genotypes CC, CG respectively. . Study of Genotype - Phenotype Association revealed significant correlation (p<0.0001) between them among comparative group as 86.9% and diabetic group as 48.8%. A comparative study of Biochemical analysis betweendiabetic and non diabetic individuals from different genotypes for TAS2R38 (P49A) gene showed significant elevated value of cholesterol among diabetic from the GG genotype 191.85 ± 6.57 mg/dl with non significant elevated values of Hb1c 8.93 ±1.77 and triglyceride 149.57±6.53 mg/dl. However, all these parameters were significantly more higher among diabetic than non diabetic . Significant differences(P<0.05) were recorded between diabetic and non diabetic according to their thyroid hormones levels with low values of T3 , T4 and high values of TSH among diabetic than non - diabetic. TSH hormone recorded its highest significant values among diabetic of CC genotype with 4.39 ± 0.16 μUI/ml as compare to the other two genotypes heterozygous CG and recessive homozygous GG . Although non significant , T4 recorded its lowest values among diabetic of the CC genotype .However, significantly (P <0.05 ),T3 hormone found to have lower values in the two homozygous genotypes; CC genotype for diabetic 0.54± 0.03 ng/ml and non diabetic 0.53±1.64 ng/ml ; GG genotype : 0.72± 0.04 ng/ml ; 1.82± 0.59 ng/ml for diabetic and non diabetic respectively . while individuals with CG genotype found to be non significantly different .Lastly it could be concluded that this trait may have some effect on BMI and may play a role the variation found among peoples in biochemical parameters (cholesterol, thyroid hormones ) changing's especially among diabetic patients. Ministry of High Education and Scientific Research Basrah University/ College of Education for Pure Science Department of Biology A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes MellitusA Thesis submitted to the Council of the College of Education for Pure Science/ University of Basrah as a partial fulfillment of the requirements for the degree of Philosophy of Doctor of Science in Biology / Genetics By Hasna Amir Mohaus BSc. Biology / 1994MSc. Biology / 1998 Supervision by Assist.Prof.Dr. Prof.Dr.Faizah A.

دراسة النسق البلازميدي وتحليل التحييد مع طيف الحساسية السريرية في Staphylococcus aureus الدوائية لعزلات مدينة البصرة == Study of Plasmid profile, Curing analysis, and Antibiotic Susceptibility Patterns of Clinical Staphylococcus aureus isolates in Basrah city

Author name: انتصار جميل حمد
Supervisor name: ميثم ايوب الحمداني
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Master
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages: