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دراسة مناعية جزيئية لبكتريا المكورات العنقودية البشروية المعزولة من بعض العينات السريرية في محافظة كربلاء == Immunological and Molecular Study of Staphylococcus epidermidis Isolated from Devices Associated Infections in Karbala Province

Author name: ريام احمد شهاب الجنابي
Supervisor name: انغام جاسم محمد | حسن علي حسين السعدي
General topic: Medicine
Specific topic: Pathological Diagnostics
Degree: Master
University: Middle Technical University - College of Health and Medical Technology
Language: English
University location: Najaf
Key words:
  • Staphylococcus epidermidis,TLR-2,IL-8
Abstract: Summary
The present study was involved 150 patients in different gender and age cases , which divided in to { Burns (50), Dialysis (38), ENT (32), Cardiovascular (30)} and 50 healthy as a control group during the period from October 2017 to February 2018. Clinical specimens were collected in the Imam AL Hussain medical city in Holy Karbala province, thirty-eight samples were diagnosed as S.epidermidis positively, while the remaining samples were S.aureus and Ps.aeroginosa.
Age mean of patients was (47.9) years, (28) of them were males and (10) females. Their ages ranged between (20-80) years. The results of Uniplex PCR of tested genes which clarified that all the S.epidermidis isolates carrying mecA gene and gene 16sRNA. On the other hand that only 33 (86.84%) isolates carrying the icaA gene.
Biofilm formation also play a role as important factor for the establishment of S. epidermidis as a nosocomial pathogen, microtiter plate assay showed that 18 (47.4%) bacterial isolates were strong biofilm producers, 15 (39.5%) were weak producers, while only 5 (13.1%) isolates were non producers .It has been concluded that the icaA gene correlated with the biofilm production in S.epidermidis as virulence markers in staphylococcal infections associated with urinary catheterization.
S. epidermidis showed different susceptibility towards antibiotics .The highest rate of resistance (100%) to Methicillin (MET), Penicillin (PEN), Cefoxitin (CX), Ciprofloxacin (CIP), Clindamycine (CLN) and Chloramphenicol (CHL), whereas was relatively lower resistance (50%) toward Erythromycin (ERY), 18.5% resistant to Vancomycin (VAN), and only (10.5%) were Gentamycin (GEN) resistant. Development of antibiotic resistance is often related to the overuse, and misuse of the antibiotics prescribed. Resistance of S. epidermidis continues to be an important clinical therapeutic problem, which can be found in an increasing multidrug resistance in these bacteria.
Also the concentration of IL-8 raised significantly (p ≤ 0.05) in all groups of patients in comparison to the healthy control group. The level of IL-8 in age group (51-60) years recorded higher percentage (12.547 pg/ml) compared with control group, whereas the concentration means of TLR2 in females was (11.261) and in males (10.452) compared with control group.
Finally, in this study it has been concluded that S.epidermidis infections occur in patients attenuated the dialysis and burns unit because the bacterial isolates show high resistant to different antibiotics types which can affect the immunological markers (TLR2 and IL-8) in blood. Also, it has been concluded that icaA gene was strongly correlated with biofilm activation, whereas mecA gene was correlated with multidrug resistance in S.epidermidis, indeed biofilm production was play a role in TLR2 activation, so resistant to antibiotics were increasing colonization of this bacteria and resistant to phagocytosis.
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