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التحليل الوراثي التبادلي الكامل والتوصيف الوراثي لزهرة الشمس باستخدام مؤشرات RAPD == Genetic full diallel analysis and genetic characterization for sunflower using RAPD markers

اسم المؤلف: حمزة محسن كاظم الخفاجي
اسم المشرف: محمد احمد ابريهي الانباري | نضال عبد الحسين مسان البديري
الموضوع العام: علوم الحياة
السنة: 2016
الموضوع الدقيق: النبات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:

تحضير مركبين نانويين هجينين من الكلورهكسدين وحامض التانيك وتحديد فعلهما المضاد للاحياء المجهرية المعزولة من مرضى الحروق في محافظة كربلاء المقدسة == Preparation of Two Nanohybrid Compounds From Chlorhexidine And Tannic Acid And Determination Their Antimicrobial Activities Against Microorganisms Isolated From Burns Patients In Sacred Karbala Province

اسم المؤلف: نورس مجيد حميد الخفاجي
اسم المشرف: علي عبد الكاظم الغانمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة عزل وتشخيص اهم انواع البكتريا المسببة لاخماج الحروق كما تضمنت ايضا تحضير وتشخيص مركبين نانويين هجينين احدهما من المطهر كلورهكسدين والاخر من حامض التانيك. تم تقييم الفعالية التثبيطية للمطهر النانوي المحضر اعلاه ضد البكتريا المعزولة في هذه | This study included the isolation and diagnosis of the most important species of bacteria responsible for the infection in burns. It also included the preparation and detection of two hybrid nano compounds, one of them from the disinfectant chlorhexidine and the other from the tannic acid. The inhibitory activity of the nano disinfectant was assessed against the isolated bacteria in this study also, the antioxidant activity of the hybrid nano tannic acid was identified and the results showed the following : 1 - As a total 32 samples were taken from burned patients hospitalized in the burning ward Imam Al - Hussien medical city in Sacred Karbalaa governate. It had been obtained 8 isolates (34.78) % from Pseudomonas spp., 7 of them were Pseudomonas aeruginosa and one was P. oryzihabitans , 4 isolates (17.39) % were Acinetobacter baumannii, 4 isolates (17.39) % were Klebsiella pneumoniae, and one isolate (4.34) % of Proteus mirabilis, Escherichia coli, Serratia liquefaciens and Chryzeobacterium meningosepticum and two isolates (8.69) % of Staphylococcus aureus in addition to one isolate (4.43) % from Candida guilliermondii yeast.2 - Chlorhexidine disinfectant and tannic acid were used in preparation of the two hybrid nano compounds after intercalating each of them on layers of double hydroxide (Mg/Al - LDH). FT - IR spectrum studies has shown the appearance of specific chemical groups and the disappearance of others while the spectrum of x - ray(XRD) has shown the appearance of new levels of diffraction in the spectrum of the hybrid nano disinfectant Mg/Al - CHX - LDH and the spectrum of the hybrid nano tannic acid Mg/Al - TA - LDH as compared to the spectrum of the Mg/Al - LDH, which indicate that the two prepared compounds under study is a nano hybrid compounds. Results of the scanning electronic microscope has shown, the change in the shape of the surface of the nanohybrid compounds that prepared above as compared to the layer of double hydroxide. It turns out from the results of the examination with the use of Atomic Force Microscope (AFM), that the diameter of the particles of hybrid nano disinfectant and hybrid nano tannic acid were (121.66 and 77.70) nm, respectively. 3 - The inhibitory activity of the hybrid nano disinfectant Mg/Al - CHX - LDH and the free disinfectant (Free CHX) has been studied and the results revealed followings : a. The hybrid nano disinfectant has the highest inhibitory activity against the two bacterial isolates P. aeruginosa (11 and 23 - A) with an inhibition diameter of 24 mm for each, while the inhibition diameter for the free disinfectant was 23.5 mm against the isolate P. aeruginosa 4 - C.b. The hybrid nano disinfectant showed the highest inhibitory activity against the two isolates A. baumannii (16 - A and 31 - A) with an inhibition diameter of 18 mm for each, while the highest inhibition activity for the free disinfectant was against the isolate A. baumannii 22 - B with an inhibition diameter of 19.5 mm.c. The hybrid nano disinfectant showed the highest inhibitory activity against the isolate K. pneumoniae (16 - B) with an inhibition diameter of 24.5 mm, while the highest inhibition activity for the free disinfectant was against the isolate K. pneumoniae 15 - B with an inhibition diameter of 24.5 mm also.d. The highest inhibition activity for the hybrid nanoic disinfectant against each of bacteria P. mirabilis and E. coli with an inhibition diameter of 23.5 mm, while the highest inhibitory activity for the free disinfectant was against the bacteria E. coli with an inhibition diameter of 24.5 mm, while the bacteria C. meningosepticum was the least affected among the other types of the isolated bacteria with an inhibition diameter of 8 mm and it was resistance to the hybrid nano disinfectant.e. The highest inhibition activity for the hybrid nano chlorhexidine was against the isolate S. aureus 21 with an inhibition diameter of 28.5 mm, while the highest inhibition activity for the free chlorhexidine was against the isolate S. aureus 22 - A with aninhibition diameter of 31.5 mm.f. The inhibitory activity of both the hybrid nano and the free disinfectant against C. guilliermondii yeast was closely to each other with an inhibition diameter of (28 and 29) mm, respectively.4. The study of antioxidant activity confirmed the following : a. Using free radical scavenging method, the IC50(Half maximal inhibitory concentration) value against ABTS free radical were at 10 µg /ml for free tannic acid while it was at 125 µg /ml for the hybrid nano tannic acid Mg/Al - TA - LDH.b. Measurement of ferric ion reducing power revealed that free tannic acid had reducing power greater than that of the hybrid nano tannic acid

دراسة تاثير الذيفان حال الدم الفا المستخلص من بكتريا Echerichia.coli في فعالية واستجابة الخلايا المناعية == Study The Effect of The Alpha Hemolysin Toxin Extracted From Echerichia Coli In The Activity And Response of Immune Cells

اسم المؤلف: وقار عدنان حمدان الكبيسي
اسم المشرف: ذكرى عدنان جواد المسلماوي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية محورين الاول الحصول على عزلات من بكتريا E.coli من المرضى المصابين بخمج السبيل البولي اذ تم الحصول على اربعة عزلات من بكتريا E.coli من مختبر الصحة العامة في كربلاء، وعزلة من بكتريا E.coli من براز شخص سليم من مستشفى الاطفال في كربلاء و| The isolation of E.coli bacteria from patients with urinary tract infection, Four isolates were obtained from the public health laboratory in Karbala, and isolation of E.coli bacteria from the feces from healthy person and S.aureus from the public health laboratory, on the basis of microscopic diagnosis and biochemical test isolates were confirmed diagnosis.This axis also included the investigation of the ability of four UTIs isolates to produce the toxin qualitatively on blood agar and measuring the diameters of hemolysis and quantitatively by measuring the amount of hemoglobin released from the RBCs.The toxin (hemolysin) was extracted from the most efficient isolate, after the cultivation in the chemically defined medum (CDM) The highest dilution gave hemolytic activity on blood cells 1/32 (320 units / ml), but after sedimentation, dialysis and freeze - drying found that (1 / 64) caused hemolysis of 50% of the blood cells compared to the standard curve.The results of this study, indicated that the role of the toxin (Hemolysin) in the pathogenecity and virulence of E.coli, lowered the lethal dose (LD50) of the bacteria which decreased from (107 × 3.16 cells / mL) to (106 × 2.34 cells / mL).The second axis included the study of the immune responce included : Studying the effect of the toxin on the immune cells isolated from the peritoneum of the mice through its effect on viability of these cells, using the concentrations of the toxin that showed the hemolytic activities (100, 90.70, 50.25) %, as well as determine the effect of the toxin on the phagocytosis S.aureus process in different periods The study found that the toxin effect on the immune cells depends on the concentration used.The low concentrations of the toxin, which showed hemolytic activity (25%) did not affect the viability of immune cells and their ability to phagocytosis where the results of the statistical analysis showed no significant difference (0.05 <p) in comparison with control, while high concentrations of the toxin showed effects on the viability of immune cells and on phagocytosis where statistical analysis significant difference (0.05> p) compared to control treatment, and the results showed that the effect of the toxin on the phagocytosis depended on the concentration and time, where phagocytic Index decrease with dose and time.It was estimated the level of cytokins (IL - 1B, IL - 2, IL - 6, TNF) in plasma were estimated for mice injected with sub lethal dose 50 (106) of the non hemolytic E.coli isolated from stool and another group which injected with LD50 of the toxin (25%) and a third group were injected with a mixture of bacteria the toxin in addition to the control group injected with normal saline, The level of cytokins during different periods of time (6, 12, 24, 48, 72) hour were estimated, where it was found that the sub LD50 of the non hemolytic E.coli had no effect on the level of TNF while sub LD50 of the toxin had no significant effect as well as the doses of the mixture in comparison with control treatment while the other cytokins, Results showed little rise in the level of IL - 1? in mice injected with non hemolytic bacteria and when the injection with sub LD50 as well as two doses mixture was large, as well as the case for IL - 2 and the IL - 6, but in different proportions, from this we conclude that the toxin stimulates the liberation of the IL - 1 and IL - 2 and the IL - 6 from immune cells, but does not affect the TNF.

دراسة مناعية وكيموحيوية لبروتينات الطور الحاد Acute phase proteins == Immunological and Biochemical study on acute phase proteins

اسم المؤلف: الاء جواد كاظم الغانمي
اسم المشرف: وفاء صادق الوزني
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:

الفعالية الحياتية لعدد من المستخلصات النباتية اتجاه بعض الفطريات المعزولة سريريا == Bioactivity of Some Plant Extracts On Some fungi Isolated Clinically

اسم المؤلف: نور شاكر مهدي الوزني
اسم المشرف: زهير حميد عبود | ابراهيم خليل حسون
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:

دراسة تاثير الزيوت الطيارة المستخلصة من بعض النباتات في بكتريا Pseudomonas aeruginosa المعزولة من خمج الحروق == Study the effect of volatile oils extracted from some plants on Pseudomonas aeruginosa isolated from burn infection

اسم المؤلف: لمى عدنان مزعل
اسم المشرف: ذكرى عدنان جواد | ابراهيم صالح عباس
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:

دراسة بعض المعايير المناعية و الكيموحيوية في مرضى داء السكري النوع الثاني == Study Of Some Biochemical And Immunological Parameters In Patients With Type 2 Diabetes Mellitus

اسم المؤلف: تغرید عبد نجم الاسدي
اسم المشرف: وفاء صادق الوزني | ھادي رسول حسن
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: كربلاء
الصفحات الاولى:

دراسة وبائية الاخماج الجلدية الفطرية واهم المضادات الفطرية المستخدمة في علاجها == Study of epidemiological Dermatophytoses infection and the best Antifungal therapy that used for treatments

اسم المؤلف: ميثم محمد علي الطريحي
اسم المشرف: زهير حميد عبود الظويهري
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:

دراسة الفعل التثبيطي لبعض المستخلصات النباتية ضد بعض الفطريات الجلدية Dermatophytes == Study of Inhibition Action of Some Plant Extracts Against Some Dermatophytes

اسم المؤلف: ليندا حميد تركي الغزالي
اسم المشرف: زهير حميد عبود الظويهري
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:

دراسة كيموحيوية لانزيم البروتييز المنتج من الفطر Beauveria bassiana == Biochemical Study of Protease Enzyme from Beauveria bassiana

اسم المؤلف: سرور محمد علي العبادي
اسم المشرف: عـلي عـبد الكاظـم جاسم
الموضوع العام: علوم الحياة
السنة: 2006
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:

تاثير البوتاسيوم والجبريلين في النمو والحاصل والمواد الفعالة في نبات الكجرات Hibiscus sabdariffa L. == The Effect of Potassium and Gibberellic Acid on the Growth,Yield and Active Ingredients of Roselle Plant (Hibiscus sabdariffa L. )

اسم المؤلف: جعفر سلمان جاسم
اسم المشرف: عبد عون هاشم علوان الغانمي
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
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