البحث — المستودع الرقمي العراقي

نتائج البحث: 13 من أصل 38

دراسة امكانية الاستفادة من تركيز بروتين المصل الفعال نوع سي بروتين التفاعلي CRP في التشخيص المبكر للاصابات البكتيرية لحديثي الولادة وتحديد توقف اعطاء العلاج من خلاله == The Study of Usefulness of C - Reactive Protein Concentration In The Early Diagnosis And Determining The Duration of Antibiotic Therapy of Suspected Neonatal Bacterial Infection

اسم المؤلف: قاسم ابراهيم حسين

اسم المشرف: ايمان ناطق ناجي البياتي

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: ان التشخيص السريع لاصابات تجرثم الدم في الاطفال حديثي الولادة من الامور المهمة وذلك لخطورة الاصابة المؤدية الى الموت.الغرض من دراستنا هو امكانية استخدام فحص سي - البروتين الفعال في التشخيص المبكرلهذا المرض البكتيري وكذلك استخدامه في تحديد نقطة التوقف عن ا | Rapid diagnosis of bacterial infections of newborn infants is very important itcause of morbidity and mortality. Aim of the studyThe aim of the studyis to examin the possibility of using C - reactive protein in the early diagnosis of neonatal sepsis, as well as its use in determining thestop point of the antibiotic treatment.Patent, Materials and methods This study was conducted at the Hospital of Ibn - Albaladi for children and women in Rusafathe Healthoffice / Baghdad.Iraq from February 2013 to February 2014.The number of patients with bacterial infection was 102 (62%) of the total number of patients (165) who were admitted to the neonatal care unit aged less than 28 days and their weights was more than 1500 g,. The number of males was 92 (56%) and the number of females was 73 (44%), the number of patient withearly onset sepsis EOS (less than three days) was 43 (42%) and with late onset sepsis LOS for (more than three days) was 59 (58%), 43 (57%) ofEOSwas normal deliveries infected and cesarean deliveries infected 33 (43%), while51 (57%) of LOS was normal deliveries infected and cesarean deliveries in LOS was 38 (43%). Blood samples were collected and divided into three portion : bloodculture, CRP andhematological profile.1 - Blood culture.Blood was collection from neonatal sepsis patientinto special blood culture bottle and incubate in bact /Alert 3 D device when growth of bacteria then subcultured on blood agar, MaCconkey agar, and incubatedusingvitek /2 to diagnose bacterial infection anddeterminetheir susceptibility to antibiotic treatment.ResultThe Compact results indicated that 63 (38%)of patient were negative and 102 (62%) were positive out of them 23 patients (22.6%)were infected with gram negative bacteria including {Klebsiella pneumonia 30%, Acinetobacterlowffi 17%, Enterobacter cloaca 13%, } while 79 (77.4%) were infected with gram positive bacteria including { staph Homins and staph hemolyticus each 28% then staph epidermidis 13% and staph aureus 10%, while strep spp was 6% }. 2 - c - reactive protein.It was done by twomethod for the same samples in order to characterize the accuracy of the diagnosis. The first method was agglutination test (method 1) the cut off value for positive result ? 6 µg % 79 (48%)were negative, while 86 (52%) werepositive, while the second method was immune test (method 2) µµµusing Nycocard device werethe cut off value for positive result ? 5 µg % the results 102 (62%) were positive while 63 (38% negative.3 - hematological profile.The hematological profile of our patient included PCV, WBC, neutrophil, lymphocyte, monocyte, count were measured using Emerald automated Hematology device. The results of these tests werenot compatible with the results of the blood culture, but normal result were compatible with negative blood cultures. The results adopted of blood culture confirm the presence of bacterial infection and then compared with theresults CRP test and blood profilefor the same samples.Negative result of blood culture and CRP test were 54 (33%) while positive results of blood culture and CRP test were recorded and improved after taking treatment for (, 3, 5, 7,) days for (40, 31, 5)patients respectively.ConclusionWeconcluded C - reactive protein test was useful in the diagnosis of bacterial blood infections and determine the stop - point otreatment. Gram negative bacterial isolates were sensitive impinim, livofloxacin and Amikacin while they were resistant to Ampicillin, Ampicillin /Sollbectam and Gentamycin. on the other hand gram positive bacteria were sensitive to Amikacin, Ampicillin/sulbactam Ciprphloxacin, and Tigecyclin and were resistant to Erythromicin, and Oxacillinand Benzylpencillin.

التحري عن التعبير الجيني لل FOXP3 وTGF - ?1 باستخدام الطرائق الجزيئية والمناعية في سرطان الرئة اللاصغير الخلية == Detection of FOXP3 Gene Expression And TGF - ?1 Using Molecular And Immunological Methods In Non - Small Cell Lung Carcinoma

اسم المؤلف: سهاد فيصل حاتم المقدادي

اسم المشرف: امنة نصيف جاسم | بان عباس عبد المجيد

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: الاحياء المجهرية

الدرجة: دكتوراه

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

المستخلص: Studies have linked FOXP3 and TGF - ? expression to the outcome of certain cancers. FOXP3 is a marker known to be expression in T - regulatory cells while TGF - ? is a secreted protein usually detected in the extra cellular matrix.The present study aimed at focusing on the identification of immune markers namely FOXP3 and TGF - ? with their expression patterns in lung cancer patients as a useful tool to predict disease progression.Also it is aimed to design molecular evaluation of m RNA expression of both FOXP3 and TGF - ? in peripheral blood mononuclear cells and bronchial (brush) cells of patients with lung cancer and benign lesions, using qRT PCR; determining the T - reg level in the peripheral blood employing the High Rsolution Melting (HRM) as a novel method to detect Treg - specific demethylated region (TSDR); molecular DNA analysis of somatic mutation of exons 3, 6, 7 of FOXP3 in patients with lung cancer tissue and benign lesions and immunohistochemical (IHC) estimation of FOXP3 and TGF - ?1 in T - reg and cancer cells in formalin fixed paraffin embedded(FFPE)lung cancer tissue and benign lesions.Blood samples were collected from 30 patients with newly diagnosed, non small cell lung carcinoma and 30 patients with benign lesions.Patients were recruited at The Specialized Surgery Hospital and Oncology Teaching Hospital/Baghdad.Samples from 16 apparently healthy donors were used as control during the period from June 2012 to June 2013. The samples preservation with TRIzol reagent were subjected to molecular study including RNA and DNA extraction; reverse transcription; RT - PCR; HRM assay and DNA sequencing were done in the Molecular Oncology Unit/Guys and ST Thomas? s hospital/ King College/London/UK.The expression level of FOXP3 was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of FOXP3 expression(fold change)was significantly high(2.64±0.09)in cancer cases than in benign cases(1.32±0.04)and healthy control(1.38±0.06)with p<0.05.A significant association between high expression level and >60 age and squamous cell carcinoma in cancer cases P<0.05.The expression level of TGF - ? was high in 16(61.5%)in lung cancer cases.A significant difference was noticed between cancer cases from one side and benign lesions or healthy control on the other side p<0.05.Mean of TGF - ? expression (fold change) was significantly high (6.27±0.56) in cancer cases more than healthy control (2.87±0.09) with p<0.05.The association was significant between TGF - ? expression level (high and low) and age>60in cancer and benign groups (p<0.05), while no significant association with gender and cancer types were noticed.For FOXP3 mRNA expression in bronchial brush cells, the result showed no significant difference between the mean fold change of malignant(3.57 ± 0.06) and benign(4.02 ± 0.06) patients. The low expression was predominated both in cancer and benign cases. No significant differences were found between FOXP3 expression (high and low) and age; gender ; cancer types..According to FOXP3 T - reg specific demethylated region detection, results showed that the mean percentage of FOXP3demethylation in lung cancer patients (4.32 ± 0.04) was significantly higher than in benign lesions (3.22 ± 0.02) patients andhealthy controls(3.33±0.04). A positive correlation coefficient with high significant, was found in the group of cancer samples (R² = 0.6653;r = 0.69;P : 0.0017)on correlating percentage of Treg and demethylation of FOXP3 from one side with its m RNA expression on the other side.In benign lesion group was(R² = 0.5334;r= 0.59;p= 0.0027), While in the control group a positive correlation but a weak significance was found (R²= 0.2383;r=0.28;P=0.0437).FOXP3 gene sequencing revealed high frequency of missense mutations c.715 GTA>CTA : V 239 L in 17 (94.44%) in malignant sample and non cancerous cases7 (87.5%)without statistical differences. Missence mutations were also detected in exone 3 in 3(16.67%) cancer cases and in 1(12.5 %) benign lesion.No missense mutations could be detected in exon 6. Intronic mutations and silent mutation were variable in three exons without statistical differences. Many cases of adenocarcinoma have shown multiple mutations either of missense or Intronic types. Missense mutations of exon 7 were correlated significantly with an age of 60?years. Exon 3 mutations were significantly associated with adenocarcinoma. Positive FOXP3 Immunohistochemistry (IHC) staining in tumor cells was associated with high missense mutations frequency 10(55.55%) in exon7, while in exone 3 was 2(11.11%). Negative FOXP3 IHC staining in the tumor cells was associated with seven missense mutations in exon7 and one (5.55%) in exon 3, in addition 4(23.53%) cases of the exon 7 missense mutations were associated with negative FOXP3 expression in lymphocytes.The result showed that FOXP3 by using IHC staining was positive in 21(70%) of nuclei of cancer cells, and 22(73.3%)in Treg infiltrates.The positive cancer cells and Treg infiltrates associated significantly with age>60 (p<0.05).No significant association was found withgender, cancer type, while there is association with moderate differentiation compared to poorly differentiation (p<0.05). High frequence of FOXP3 expression score 3 and high intensity were appeared in nuclear cancer cells compared to benign lesions cells, while Treg infiltrates with score 1 and high intensity was high frequency in malignant and benign.The result showed that TGF - ?1 by using IHC staining was positive in 25(83.3%) in the cancer cells and 21(70%) in stromal cells. No significant difference was noted between positive expression in malignant and benign lesions p>0.05.No significant association was noticed between positive cells expression and age, gender, cancer type and differentiation p>0.05. High frequency of TGF - ?1 expression score 3 and high intensity in malignant cells and benign. Also stromal cell expression score 3 and high intensity were predominated in malignant and benign lesions. The high and moderate intensity expression was more frequent in matrix surrounding cancer cells compared to non cancerous.Total agreement and kappa coefficient between FOXP3 and TGF - ?1were poor in malignant and benign epithelial cells and stroma, while the perfect agreement was between expression of TGF - ?1in stromal cells

التاثير التثبيطي لمستخلصات بعض النباتات الطبية في البكتريا المعزولة من حالات التهاب المجاري البولية == The Inhibitory Effect of Some Medicinal Plants Extracts on Isolated Bacteria From Urinary Tract Infection

اسم المؤلف: صبا طالب هاشم

اسم المشرف: مهـدي ضمـــد القيســي | جنان مجيد العقيدي

الموضوع العام: علوم الحياة

السنة: 2009

الموضوع الدقيق: الاحياء المجهرية

الدرجة: دكتوراه

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

التاثيرات التازرية لبعض المستخلصات النباتية والمضادات الحيوية في بكتريا Staphylococcus aureus المعزولة من البلعوم الفمي == Synergistic effects of plants extracts and antibiotics on bacteria Staphylococcus aureus isolated from oropharynx

اسم المؤلف: سيماء عمار ناجي

اسم المشرف: الهام سعيد عبد الكريم بنو

الموضوع العام: علوم الحياة

السنة: 2012

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

العوامل المسببة للاسهال لدى الاطفال في اربيل و تاثير بعض المستخلصات النباتية على العزلات البكتيرية == Causative Agents Of Diarrhea In Erbil Children And The Effect Of Some Plant Extracts On Bacterial Isolates

اسم المؤلف: سوسن محمد عبد الله السورجي

اسم المشرف: انتصار محمود جمعة | عباس عبد القادر رباط

الموضوع العام: علوم الحياة

السنة: 2009

الموضوع الدقيق: الاحياء المجهرية

الدرجة: دكتوراه

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

عزل وتشخيص المبيضات البيضاء Candida albicans من المهبل ودراسة عوامل ضراوتها وحساسيتها للمضادات الفطرية == Isolation and Idintification of candida albicans from vaginal and Study of Virulance Factors and sensitivity to Antifungal Agents.

اسم المؤلف: شيماء نغيمش مزعل الحجامي

اسم المشرف: الهام سعيد عبد الكريم | اياد فضيل نقاش

الموضوع العام: علوم الحياة

السنة: 2004

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

تاثير مستخلصات نبات خناق الدجاج Zygophyllum fabago L. والزيت الطيار لقشور ثمار نبات النارنج Citrus aurantium L. الخضراء في نمو وفعالية بعض الاحياء المجهرية == Effect of plant extracts from Zygophyllum fabago L. and essential oil from green peel of Citrus aurantium L. fruits on growth& activity of some microorganisms

اسم المؤلف: استبرق عز الدين محمود القيسي

اسم المشرف: بتول زينل علي | الهام سعيد عبد الكريم

الموضوع العام: علوم الحياة

السنة: 2004

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

عزل وتشخيص الاعفان والخمائر الملوثة للمستشفيات في مدينه الديوانية == Isolation and Identification of Fungi and Yeasts that Contaminates and Causes Nosocomial Infections in Diwaniya City

اسم المؤلف: هيام قائد محمد الكناني

الموضوع العام: علوم الحياة

السنة: 2005

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

دراسة الاصابات البكتيرية في الجهاز التنفسي لمرضى زرع الكلية == STUDY THE BACTERIAL INFECTIONS OF RESPIRATORY SYSTEM IN KIDNEY TRANSPLANT PATIENTS

اسم المؤلف: عباس عطية حمودي علي العامري

اسم المشرف: مي مجيد عبد الله | لازم حميد الطائي

الموضوع العام: علوم الحياة

السنة: 2005

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

دراسة وبائية فصلية للاصابات المهبلية في بغداد == Epidemiological Seasonal Study of Vaginal Infections in Baghdad

اسم المؤلف: زينة هاشم شهاب العاني

اسم المشرف: مي مجيد عبد الله | جنان مجيد العقيدي

الموضوع العام: علوم الحياة

السنة: 2005

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

تاثير عصائر الليمون الحامض Citrus limon L. والفلفل الاخضر البارد Capsicum grossum L. والبصل الابيض الحلو Allium cepa L. في انواع من البكتريا المعزولة والملوثة للسلطة == Effect of Juice of Limon (Citrus limon L.) ; Capsicum (Capsicum grossum L.) and Onion (Allium cepa L.) on Isolated and Contaminated Bacterial Strains from Salad

اسم المؤلف: صلاح صديم صالح الدوري

اسم المشرف: مهدي ضمد محيسن القيسي

الموضوع العام: علوم الحياة

السنة: 2005

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى:

اخماج المستشفيات الناتجة عن المكورات العنقودية الذهبية في محافظة ديالى == Staphylococcus Aureus Nosocomial Infections In Diyala Province

اسم المؤلف: عهود عقيد راضي

اسم المشرف: مي مجيد عبد الله | عبد الرزاق شفيق حسن

الموضوع العام: علوم الحياة

السنة: 2005

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى:

دراسة عن تاثير الكحولات الاليفاتية والسايكلوهكسمايد في سلالات برية وطافرة حميدة الشايزوسكارومايسس بومبي == a study on the effect of aliphattic alcohols and cycloheximide against the wild type and resistant strains of schizaccharomyces pombe

اسم المؤلف: سلوى عبد الرحمن زيدان العكيلي

اسم المشرف: حامد مجيد الزيدي

الموضوع العام: علوم الحياة

السنة: 1988

الموضوع الدقيق: الاحياء المجهرية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: العربية

مكان الجامعة: بغداد

الصفحات الاولى: