Author name:
نضال ابراهيم لطيف الحداد
Supervisor name:
عبد علي ذاكر | عماد عبد الرحمن محمد صالح
University location:
Anbar
Abstract:
Study includes following up of measuring the total dissolved protein and enzymes in different body organs and the structural changes (Histological)occurring in the testis and epididymis and liver and kidney of the male mice, in addition, the study includes following up of must the hematological and Hormones changes.This study consists of three experiments.The first experiment involved exposing male mice directly for thirty consecutive day to three different magnetic stressed 750, 1500, 3000 Gauss The second experiment involved exposing pregnant mothers directly during pregnancy, amounting to 21 consecutive days for the same intensites and after the end of exposure and the occurrence of birth was taking male embryos left until puberty to conduct the study by the third experiment involved SMF same intensites also for a period of 30 days after the end of the period of exposure was that males paired with females and left until the birth then was taking male embryos left until adulthood to conduct the study at the end of each experiment and weighed the animals and took blood samples and explained and eradicated all organs (liver, kidney the heart and spleen) for the first test and (liver and kidney) for the second and third as the experience was detected changes in the total dissolved proteins amount were observed using the method of Biuret and those on protein band resolved on polyacrylamide electrophoresis PAGE.Also the effect of SMFs on the activity of each of the enzymes ACP, ALP, ALT and AST was estinmated using the Spectroscopic method. Electrophoresis was used to observe effects on enzymic bands of Esterase on PAGE. In addition to biochemical variables included enzymatic antioxidants, which included enzymatic SOD, CATand MDA as has been the follow - up blood changes which included the amount of hemoglobin Hb, PCV, WBC, RBC and platelet counts and blood Were estimated as the concentration of each of the hormones (prolactin, LH, FSH) and testosterone hormone as eradicated all of the right testis and epididymic for the first experiment and excised portions of the liver and kidney for the second and third experiment and they were used to prepare histological sections for the histological studies.The tissues were sectioned and stained with Haematoxylin and Eosin stain using the routine histological techniques.The result of the study could summarized as follows : - - The Results of this study showed was an increase not significant (p?0.05) in body weight It also showed decrease not significant (p?0.05) In the liver and spleen weight either kidney, heart weight did not change in any weights appears when 750 and 1500 gauss either at 3000 gauss intensity was significantly increased and clear (P?0.05) compared with control animals.—There was a significant in (MDA, AST, ALT, ACP, ALK, TP) To extract the liver, kidney, heart and spleen male mice exposed to three different stressed 3000, 1500, 750 gauss As for the enzymes(CAT, SOD) Showed a significantdecrease in heart, kidney, spleen extract to mice exposed and rise significantly in the liver of mice exposed to stressed above the first experiment, there was no significant increase in the activity of enzymes (ACP, ALK, TP) And an increase in the activity of moral enzymes(MDA, SOD, CAT, AST, ALT) In liver extract and kidney male mice second experiment compared with control animals. The third experiment, there was no significant increase in the activity of enzymes (ACP, ALK, TP) And an increase in the activity of moral enzymes(MDA, SOD, CAT, AST, ALT) Liver extract in male mice third experiment compared with the control animals in either extract kidney male mice third experiment, there was not significant increase in (SOD, CAT, AST, ALT, ACP, ALK, TP) And a significant increase in the activity of the enzyme (MDA compared with control animals - The results showed some changes in the intensity and number of protein bands to extract four organs first experience at three 3000, 1500, 750 gauss compared with animal control and extract the liver and kidney for the second test and third at all intensities when electrophoresis was carried out on PAGE. - The results showed that there are some changes in the intensity and number of enzyme bands esteras in the extract of the four organs of the first experiment at 3000, 1500, 750 gauss compared with animal control and extract the liver and kidney for the second test and third at all intensities when electrophoresis was carried out on PAGE. - Results of the first experiment showed significant increase (P?0.05) in each of the(Hb, PCV, PLT and RBC) Animals exposed to either 1500.750 gauss at 3000 gauss intensity rise was not significant (P?0.05) compared with control animals. The results showed a significant decrease (P?0.05) in WBC at all intensities compared with control animals at all intensities.Results of the second and third experiment showed not significant increase (RBC, PCV, Hb and PLT) and not significant (P? 0.05) in WBC at all intensities compared with control animals at all intensities. - The results of the first experiment showed that there was significantly higher (P?0.05) in the LH hormone when exposed to the three stressed 3000, 1500, 750 gauss compared with control animals. there was a significant decrease (P?0.05) in each of the hormonal FSH prolactin When the exposed to the three stressed 3000, 1500, 750 gauss compared with control animals, but the Testosteron hormone has decreased at 750 gauss a significant decrease (P?0.05) compared with control animals. - The results of the first experiment not significant decrease (P?0.05) in the testis and epididymis weight of the animals exposed to 1500 and 750 gauss compared with control animals either at 3000 gauss intensity, there was a significant increase (P?0.05) in both organs weight compared with control animals. Histological studies showed the significant decrease in the diameter and thickness of epithelial tissue of the seminiferous tubules and epididymis tubules and duct efferentes inthe 750and 1500 Gauss/where appeared where appeared in epithelial tissue is incomplete and thinner than in the animal control either when the intensity of 3000 gauss there was a significant decrease diameters and high padded her cells and appeared in epithelial tissue integrated compared with animals control. - Histological study showed the liver of male experience the second occurrence of some histological changes in the central vein and semi - sinus blood and prepare Kupffer cells and epithelial cells lining compared with control animals. And the lack of any histological changes in male mice kidney second experiment and stressed 3000, 1500, 750 gauss compared with control animals.Histological study also showed the liver third male mice experiment that no histologic changes at 3000, 1500, 750 gauss compared with control animals either the kidney of male mice have shown some changes in some histological structures at 750, 1500 and 3000 Gauss, compared with control animals
