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دراسة لتشخيص بكتريا الملوية البوابية Helicobacter pylori المعزولة من الخزع النسيجية والغائط Study For Diagnosis Helicopacter Pylori Isolated From Biopsy And Stool

اسم المؤلف: ابرار علي حسين

اسم المشرف: هيام عبد الرضا كريم العواد ياسمين خضير الغانمي

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: الاحياء المجهرية - البكتريا

الدرجة: ماجستير

الجامعة: جامعة كربلاء - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: كربلاء

الصفحات الاولى:

المستخلص: تناولت الدراسة الحالية عزل وتشخيص بكتريا (Helicobacter pylori) من الخزع النسيجية وعينات البرازالماخوذة من المرضى المراجعين مستشفى الحسين التعليمي حيث جمعت 122 عينة من الخزع النسيجية من وحدة الناظور من 61 مريض بواقع عينتين للمريض الواحد, كما تم جمع 228 عين | The present study addressed isolate and diagnose the bacteria (Helicobacter pylori) from biopsy and stool, samples taken from AL - Hussein Teaching Hospital where collected 122 samples of biopsy from the endoscopy. From 61 patients by two samples per patient, also were collected 228 samples of stool from the laboratory of the Department of parasites by two samples of 114 patients for the period from January 2014 to January 2015. Where shown on biopsy results which were subject to examination by the rapid urease test (RUT) the presence of 60 (98.4%) cases, while there were 23 (37.7%) case of those bacteria being positive related to biopsy was not significant differences between the two tests performed. When the distribution of test results (RUT) and culture according to age group, it appear that the highest percentage of infection was in 50 - 41 age group that which showed the highest percentage on examination (RUT) of the number of infected at 16 (26.23%) cases, either in the culture technique number of infected at 9 (14.75%) cases. But when the results of each of the examination (RUT) and culture were distribution according to the sex of the patient's infection was the higher in the females than males reaching males 28 (45.90%) cases in females of 32 (52.46%) cases for examination (RUT). No significant differences appear between the sexes in terms of infected. While the males in 8 (13.11%) and in the case of females was 15 (24.59%) case for the culture technique it did not show significant differences between the sexes in terms of the patients. While taking stool samples to check the stool antigens, it was found that there are 76 (66.7%) cases diagnosed while the technology for the polymerase chain reaction gene cagA to 49 (43%) cases have significant differences between the tests performed. When distribution the results of each of the stool antigens and polymerase chain reaction technique by age group category the highest infected was in 46 - 55 the age group with the number of 23 (20.18%) people case for screening stool antigens. The test results of the technology polymerase chain reaction showed that highest rate of infection was in the 35 - 26 age group where the number of patients was at 15 (13.16%) case.The results of the stool antigens examination also showed that the infection rate among males was higher than females, 39 males (34.21%) and in the case of females has reached the number of 37 cases (32.46%) casesThat did not show significant differences between the sexes patient. The technical results of the polymerase chain reaction by sex has been the injury was higher in females than males reaching 22 in males (19.30%) cases, while in females was 27 (23.68%) cases did not show significant differences between the sexes in terms of the infected. The results of the study conclude that (RUT) and stool antigen test is the fastest in the detection of infection from the bacteria H. pylori and then comes beyond culture technique for biopsy sample and then polymerase chain reaction for stool samples

دراسة جزيئية ومناعية لطفيلي اللشمانيا الجلدية Cutaneous Leishmania في المحافظات الوسطى والجنوبية Molecular And Immunological Study of Cutaneous Leishmania In The Middle And Southern Provinces

اسم المؤلف: ازهار موسى جعفر الموسوي

اسم المشرف: علي حسين الكبيسي مهدي حسين العمار

الموضوع العام: علوم الحياة

السنة: 2015

الموضوع الدقيق: علوم الحياة

الدرجة: دكتوراه

الجامعة: جامعة كربلاء - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

النص الكامل من المصدر: https://uokerbala.edu.iq/wp-content/uploads/2020/06/Rp-Molecular-and-Immunological-study-of-Cutaneous-Leishmania-in-the-middle-and-southern-provinces-.pdf

مكان الجامعة: كربلاء

الصفحات الاولى:

المستخلص: The study includes distribution of the Cutaneous Leishmania in some governorates in Iraq. Specimens are included cases of outpatients in hospitals like : Al - Karamah, Al - Sadir Teaching Hospital in Baghdad, Al - Hilla Teaching Hospital, Al - Hussein Teaching Hospital and Ein Altamer General Hospitals in Kerbala, Al - Sadir Teaching Hospital in Al - Najaf, Al - Karamah and Al - Zahraa Teaching Hospitals in Wasit, Al - Diwanyia General Hospital. Al - Hussein General Hospital in Al - Nasiriya, Al - Smawa General Hospital, Al - Sadir Teaching Hospital and Al - Qurna General Hospital in Al - Basrah during October 1122 to march 1121 Cutaneous Leishmania is consider a major problem that faces the patients because it causes deformities in the infection region. The study includes 330 cases of skin ulcer in which 225 of them were microscopically positive. Parasitic growth was done using two types of cultural media NNN and RPMI - 1640.The results have shown a considerable difference between male and female in which male was significantly exceeding 131) %22.11 (and female was 94) %72.44 (and 139) %72.44 (of outpatients from rural areas increased in numbers from outpatients of urban areas, and only) %12.11(from urban areas.The number of ulcerations in body has exceeded infection, that means more than one ulceration in male 83 (85%) and for female 41 (62%), and concerning infection in three or four ulceration, it is approximately equal and normally distributed on face areas 99 and a percentage of (44%) then lower arms are 92 and a percentage of (40.80%) and the upper parts are 33 and a percentage of (14.66%). Also, one infection was recorded on The study shows that 46 patients in a percentage of (%20.44) having a dry infection; whereas 179 patients (%79.55) having the wet type.For accurate diagnosis of Leishmaniaa species distributed in Iraq, PCR Was used technique and diagnosed two types of Leishmania parasite that causes the illness. A band in a length of 560 bp in 186 samples related to Leishmania major and the band sample was 750 bp related to 39 Leishmania tropica.Through studying the samples in Kerbala City, the number infected samples were 125 for the years of 2010 - 2011 the actuality is (73) infection and (52) infection from 2011 - 2012 in a ratio of (58.4 % and % 72.7 accordingly) in different inhibited areas in the governorate in which (51)) %71.2 (was in Ein Al - Tamir Suburb, and 29 cases of) %11.1 (in Al - Husseinya Suburb, as well as 17 cases of (13.6%) in Al - Hur Suburb, 16 cases) %21.2 (in the south quarters and 7 cases of (5.6%) in the north quarters and only 5 cases of (4%) in the city center.The molecular diagnosis in Polymerase Chain Reaction for Kerbala governorate samples have shown 31) %17.2 (of L.tropica, 22) %24.7(of them were male and 9 of) %4.1 (of them were female. L. major was 94 of) %42.1 (in which 55 of) %77 (were in male and 39 of) %12.1 (were in female.The immunological study for the patients serums with L. major by ELISA technique has significantly shown raised values of IgG and IgM during infection in comparison with the control group, then it declines slowly after treatment in which IgG was in ratio (1811.1 ± 523.1 mg/dl), and IgM was in ratio (166.7 ±23.6 mg/dl). Also, in L. tropica - IgG was in ratio (1722.1 ± 524.0mg/dl) and IgM was in ratio (25.3±182.9mg/dl) in comparison with control specimens, and then the ratios have declined after treatment dosages with Pentostam drug.The cellular dynamics values have shown an abstract increase in which interferon - gamma (IFN - ?) in infected patients with cutaneous L. major were (221.1±2.2mg/dl) which declined after treatment abstractly to (7.12±1.1mg/dl) as well as for L tropica (22.1±7.2mg/dl) which shows no significant abstract difference after treatment with control.An increase appears in cytokine IL - 10 that reached in L.major (122.1±8.2mg/dl) and after treatment was (8.11±2.2mg/dl) and in L. tropica was (222.1±2.2mg/dl) with no significant abstract difference after treatment with control specimen.As a simple attempt to find a vaccine for Leishmania disease a Lipophosphoglycan was isolated and purified as known factor for promastigot stage. The vaccine injected for two groups of Bulb mice in a concentration of purified vaccine (L.majorand Ltropica) to study the immunological response by detecting the Lymphocyte Transformation assay, the Delayed Type Hypersensitivity test and the PhagocytosisIndex.In lymphocyte transformation the ratio of L. tropica was % 7.2 and for L. major was % 21.7 and there is significant difference by P? 0.05 in comparison with control specimen in which its ratio was %7.4, and for Delayed Hypersensitivity test study the average foot thickness injected with vaccine and the other foot which injected with phenol - saline solution - only (1.11 ± 2.12ml) for the first group and (1.21 ± 2.72) for the second group, and for the control group it has registered (1.77± 1.15 ml) with a considerable difference of P? 0.05. By calculating the ratio of Phagocytosis Index cells, it was 17% and 27.6% for the first and second groups while for the control group it has been reaching 9.10%. Therefore, we can get advantage from these values as indicators for the rule of vaccine in the immunological response and triggering of vaccine idea for this disease.