Author name:
خديجة عبيس حمود الخالدي
Supervisor name:
خيري عبد الله داود العكيلي | هادي مدلول الميالي
Abstract:
تضمنت الدراسة الحالية الكشف عن طفيلي المقوسة الكوندية Toxoplasma gondiiفي اربع انواع من الطيور ضمت الدجاج المحلي Gallus gallus domesticus والديك الروميMeleagri galbpavol والاوز الاربد Anser anser والبط المحلي Anas platyrhychos domesticus في منطقة الفرات ا | The current study was conducted to detect of Toxoplasma gondii in five species of avian including, Gallus gallus domesticus, Meleagris galbpavol, Anser anser, Anasplatyrhychos domesticus in middle Euphrates provinces of Iraq, also in cats in AL - Diwania province, during 2011 - 2013 by using serological tests (Latex, Rapid test cassette) and molecular test (conventional - PCR and Nested - PCR) as confirmative tests to detect of B1gene which was specific gene of T.gondii in blood and Tissue samples of different organs such as Liver, Heart, Brain, Eye, Lung, Pectoral muscles, Small intestine, Spleen, and Leg muscles of avian and cats which were seropositive cases of Rapid test cassette, also the study was included the detection Oocysts of T.gondii in soil samples of ten cities from Al - Diwania province by using sedimentation and floatation methods as primarily diagnosis, also using the polymerase chain reaction to detect of B1gene in these samples. A total of 320 Avian collected, include 80 samples of each species (20 samples of chicken, Turkey, geese, and Ducks) of each province of middle Euphrates (AL - Diwania, Babylon, AL - Najaf and Karbala), in addition to 10 samples of cats and 100 samples of soil from the center of AL - Diwania province, samples of avian and cats were involved to serological test such as LAT, the results showed there were 96 positive samples in percentage %30, include, 31(%38.75) of chicken, 28 (%35) of turkey, 22 (%27.5) of geese, 15 (%18.75) of ducks and 5 (%50) of cats, the higher Percentage (%23.95) of infection with T. gondii in avian was in titer 1/160 and the lower percentage (%3.73) in titer 1/640, Whereas in cats, the higher percentage in titer 1/20 and lower percentage in titers 1/40, 1/80, 1/160 which reaches %20. The results of Rapid test cassette were showed there were 60 out of 320 samples in percentage %18.75, include 24 (%30) of chicken, 19 in (%23.75) of turkey, 10(%12.5) of geese, 7(% 8.75) of duck and 3(%30) of cats. Also the results of Rapid test cassette were showed the higher percentage of types of antibodies in the serum of avian was %36.66 of IgG +IgM together and the lower percentage was %28.33 of IgM, whereas in cats, the higher percentage was %66.66 of IgG and the lower percentage was %33.34 of IgG +IgM together. To confirm the infection of avian by T.gondii, Polymerase Chain Reaction was used to detect the B1 gene (399 bp) in 320 blood samples of avian and 10 samples of cats, the results were showed there were 34 samples (%10.62) of avian gave positive result, include, 14 (%17.5) of chicken, 12 (%15) of Turkey, 8 (%10) of geese, whereas the results were showed the B1 gene was not present in the blood samples of ducks and cats. The results of Latex, Rapid test cassette and polymerase Chain Reaction were showed, the higher percentage was recorded in chicken % 17.5, %30, %35, whereas the lower percentages were %18.75, %8.75, %0 in D ucks by using the three tests, respectively. According to the results of Rapid test cassette (IgG, IgM +IgG), the results of polymerase Chain Reaction to detect the B1 gene in 16 samples of avian (4 samples of each species) out of 39 samples and 3 samples of cats, showed there were 9 samples (%56.25), include 4 (%100) of chickens, 3(%75) of turkey, 1(%25) of geese, and 2(%66.66) of cats and the higher percentage of B1gene (399bp) in livers of avian was %55.55, %22.22 in pectoral muscles then heart, spleen and small intestine in percentage %11.11 for each one of them, whereas in cats the percentage were % 66.66 in liver while in brain and kidney the percentage was %33.34 of each one of them.. The results of Nested Polymerase Chain Reaction for amplification the gene B1 in 12 blood samples (4 samples of each species of birds except ducks) was positive for conventional - PCR, Showed the amplification occurs in 4 samples in percentage % 33.33 during the first round of reaction and 3 samples in percentage %75 in the second round, in 10 samples of tissues, the amplification occurred in 6 samples in percentage %60 during the first round and in 4 samples in percentage % 66.66 during the second round of reaction. Also the results were showed the percentage of T.gondii antibodies in serum of four types of avian (except pigeons) according to the provinces were %28.75 in AL - Diwania, %40 (Higher percentage) in Babylon, %12.5(Low percentage) in AL - Najaf and %38.75 in Karbala by latex test, whereas the percentages %26.25 (higher percentage), %10 (lower percentage), %12.25, %17.5 in each of Karbala, AL - Najaf, Babylon, AL - Diwania province respectively by Rapid test cassette, and by using polymerase chain reaction, the percentages were %16. 25 (Higher percentage), %5(Lower percentage), %6.25, %15 in AL - Diwania, Babylon, AL - Najaf and Karbala province, respectively. Also the results showed, the percentage of infection in AL - Diwania province the samples of rural areas were higher (%37.5, %22.5, %17.5) whereas the percentages were lower (%20, %12.5, %12.5) in urban areas by using latex, rapid test cassette, and polymerase chain reaction, respectively. The results of comparison between two serological tests and Polymerase Chain Reaction, showed the percentages of infection were % 10.62 (34), %18.75 (60), %30 (96) by using Polymerase chain reaction, Rapid test cassette, latex test, respectively, also the sensitivity and specificity of latex test were %35, %72 whereas the sensitivity and specificity of Rapid test cassette were %35, %88, respectively in compared with standard Polymerase Chain Reaction. In the soil samples, the percentage of presence of Oocysts of T.gondii was %10 by using floatation method and %4 by conventional Polymerase Chain Reaction. The study of grossly and microscopy pathological changes in avian and cats which infected with T. gondii, was showed there were lesions on each of liver, heart, pectoral muscles, small intestine, brain and kidney.
