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دراسة مناعية عن العلاقة المحتملة لداء المقوسات مع وجود مصفوفة انزيم الزنك 2، بروتين الصدمة الحرارية 40 والانترلوكين في النساء اللواتي عانين اسقاطا == Immunological study for possible association of toxoplasmosis with the presence of MMP2, Heat shock protein 40 & interleukin in women with miscarriages

Author name: رعد عجم صايل الجوراني
Supervisor name: باقر عبيس سلطان
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine
Language: English
University location: Najaf
First pages:

علاقـة بعض المعــلمات المناعــية والجــينية مع مرحلة المرض لمجموعة من المرضـى العراقيين المصابين بابيضاض الدم اللمفاوي المزمن == Correlation of some immunological and genetic parameters with the staging of disease in a set of Iraqi patient with CLL

Author name: انتصار شاكر علي
Supervisor name: سلمان عزيز عدوس | استشاري غسان احمد خليل
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine
Language: English
University location: Najaf
First pages:

الكشف عن حمى مالطا الانسان بواسطة استعمال بعض الفحوصات المصلي والجزئي في محافظة النجف == Detection of Human Brucellosis by Using Some Serological and Molecular Assay in Al-Najaf Province

Author name: كفاح فاضل حسون
Supervisor name: وسن سامي حميد
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine
Language: English
University location: Najaf
First pages:

النمط الجيني HLA-DQ والحساسية للاصابة بطفيلي Toxoplasma gondii بين النساء الحوامل والمجهضات في محافظة النجف == HLA-DQ Genotyping and Susceptibility to Infection with Toxoplasma gondii Among Pregnant and Aborted Women in Najaf City

Author name: رنا فاضل عبيد هلال الكلابي
Supervisor name: باقر عبيس سلطان | صباح نعمة الفتلاوي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine
Language: English
University location: Najaf
First pages:

دراسة جزيئية ومناعية لترافق وتاثير عصيات السل الرئوية سرطان المثانة == Molecular and Immunological Study for the Association and Effect of Mycobacterium tuberculosis on Urinary Bladder Cancer

Author name: زينا محمد عبد الحسين الشامي
Supervisor name: هاشم رحيم طارش | اسعد عبد الحمزة الجنابي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine
Language: English
University location: Najaf
First pages:

دراسة جزيئية ومناعية لبعض جينات الملوية البوابية == Molecular and Immunological study of some virulent genes of Helicobacter pylori

Author name: اسراء سعيد عباس كاظم
Supervisor name: كريم ثامر مشكور
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine
Language: English
University location: Najaf
First pages:

بعض الاختبارات الجزيئية والمصلية للكشف عن مرضى داء المقوسات لدى النساء اللاتي عانين اجهاضا == Some Serological and Molecular tests used to identify Toxoplasmosis among Women with Abortion

Author name: كريم غالي محمد
Supervisor name: سلمان عزيز الجبوري | باقر عبيس سلطان
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Kufa - Faculty Of Medicine
Language: English
University location: Najaf
First pages:

تاثير التنوع الجيني ل 17-il في مرضى التدرن الرئوي في محافظة بابل == Effect of IL-17 Gene Polymorphism in Tuberculosis Patients in Babylon Province

Author name: مصطفى جواد عبد خضير
Supervisor name: محمد عبد كاظم السعدي | ايفاد كريم عبد الشبلي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Babylon - Faculty Of Medicine
Language: English
University location: Babylon
First pages:

عزل جراثيم الزائفة الزنجارية في العزلات من مرضى عدوى المستشفيات والكشف عن المورثات المقاومة لمضادات البيتالاكتم وتحليل تتابع الجينات في محافظة البصرة.العراق == Isolation of Pseudomonas aeruginosa from Patients with Hospitalized Infection and Detection of ?-lactam Resistance with Their DNA Sequencing in Basra Iraq

Author name: صبا عدنان عبد الوهاب
Supervisor name: سندس صديق بكر | زكي عبد الجبار الفداغ
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University Of Basrah - Faculty Of Medicine
Language: English
University location: Basrah
First pages:

التمنيع الجيني ضد اللشمانية في الفئران المختبرية نوع Balb/c == Genetic Immunization Against Leishmaniasis In Balb/c Mice Model

Author name: وميـض هاشــم عباس
Supervisor name: ناظم كاظم مهدي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University Of Basrah - Faculty Of Medicine
Language: English
University location: Basrah
First pages:

عزل وتجنيس فايروس مرض نيوكاسل محليا من قطعان الدواجن المصابة طبيعيا في شمال العراق == ISOLATION AND GENOTYPING OF LOCAL NEWCASTLE DISEASE VIRUS ISOLATED FROM NATURALLY INFECTED CHICKENS IN NORTHERN IRA

Author name: احمد ابراهيم احمد
Supervisor name: شوني ميخائيل اوديشو | رباح نجاح جبار
General topic: Veterinary Medicine
Specific topic: Microbiology
Degree: Doctorate
University: University of Baghdad - College Of Veterinary Medicine
Language: English
University location: Baghdad
First pages:
Abstract: Newcastle disease (ND) has been concerned as one of the most important devastating diseases of poultry because of its wide range host of birds and worldwide distribution with severe economic losses in domestic poultry, due to the severe nature of the disease, ND is included as a list A disease.
Iraq has been endemic for Newcastle disease virus (NDV) with natural infection causing significant losses in the poultry industry since 1968 by the NDV/AG68 strain (AF001108) from infected chickens at Abu Graib in Iraq.
Currently, NDV genotype VII are most frequently associated with outbreaks of ND in the Middle East and Asia which have showed higher mortality in vaccinated poultry. The main objective of this study was to determine the biological and molecular characterization of the pathogenicity and genotyping followed by phylogenetic analysis. To evaluating the degree of genetic diversity of local isolated NDV strains and estimating the relationships to that NDV presence in the neighboring region, as well as preparation of inactivated vaccine against NDV from local strains was determine.
At the beginning, this study was carried out to isolate Newcastle disease virus (NDV) in naturally infected chickens of poultry farms in the north of Iraq. The study initiated diagnosis of 46 farms out of 96 broiler farms exhibited Newcastle disease (ND) (26 in Erbil, 14 in Sulaymanhia and 6 in Duhok) based on clinical signs corresponding with (8-15%) mortality and gross lesions of scarified chickens. The rapid test for detection NDV antigen from infected chickens for tracheal swab indicated that 32 farm samples were positive out of 46 initiative diagnosed farms.
Based on intiative diagnosis the identification of local isolates of NDV samples was pooled lung with trachea, spleen, cecal tonsil and intestine, were pooled in nine samples, then tissue homogenates were made from all samples in sterile Phosphate Buffered Saline (PBS) containing antibiotics, after centrifugation of tissue homogenates the supernatant fluids were assessed to real time reverse transcriptase- Polymerase Chain Reaction (RRT-PCR) assay. The results indicated that six
homogenate samples were positive for NDV and negative for Avian Influenza Virus.
After that, the remain supernatant fluids were used for virus isolation in embryonated chicken eggs then harvested allantoic fluids were used for employed for the hemagglutination activity. Six samples were showed positive results. Furthermore, virus identification was also performed by using conventional diagnostic methods such as Hemagglutination(HA) of red blood cells followed by Hemagglutination Inhibition (HI) test applying the prepared hyper immune serum(HIS) against NDV antigens to identify the NDV. The serological results demonstrated that six samples of NDV were positive, as well as the (rt-PCR) assay was performed for allantoic fluid to insure the presence of NDV using specific primers and probes to amplify specific region in the M gene of the viruses. The results revealed that six isolates were positive for NDV.
In addition, the pathogenicity of the six isolates was performed according to standard methods including Mean death times (MDT), Intra-cerebral pathogenicity index (ICPI) and Intra-venous pathogenicity index(IVPI), which revealed that six isolates were compatible to mesogenic (1/6) and velogenic (5/6) types.
At the second step, the molecular characterization of six isolates were investigated in Borehingher laboratory (Germany). The RT-PCR successfully amplified a 436 bp DNA fragment covering a part of the matrix (M) gene, fusion (F) gene (lentogenic) and fusion (F) gene (subgenotype VIIi) from all six isolates (NDV). The result was indicated that 5/6 were positive for velogenic NDV strain of genotype VIIi and 1/6 were negative. The nucleotide sequences of F gene were also determined, where the portion of the F2 gene of five isolates compared with that of other NDV strains retrieved from GenBank. Phylogenetic analysis showed that the isolates NDV belong to genotypes VIIi.
However, in local laboratory the result of FAST one-step quantitative Polymerase Chain Reaction (qPCR) successfully amplified a 525 bp fragment covering a part of the fusion (F1) gene and 515 bp fragment covering a part of the fusion (F2) gene from all six isolates. The nucleotide sequences of the PCR product of E1, S1 and S2
were found in F1 gene, while E1, E2, S1, S2, and D2 found in F2 gene out of six NDV isolates. Those results were compared with other NDV strains which retrieved from GenBank. Phylogenetic analysis showed that the Iraqi isolates are belonging to genotypes VII.
Finally, Inactivated vaccine was prepared locally using Velogenic NDV genotype VIIi isolate as a seed virus ELD50/0.1ml109 then compared with a commercial inactivated vaccine in an experimental study. This study was conducted by purchasing 120 broiler chicks and divided into three equal groups. Group 1 as unvaccinated control group , G2 for commercial vaccine and G3 for local vaccine), G2 and G3 were injected s/c with inactivated vaccine at 3 days old followed by a booster dose with LaSota live vaccine eye drop. Indirect ELISA technique was used to measure of antibody titer at ages 7, 17 and 27 days old (pre-challenge), the result showed at 7 days-old were (4251±1078) for all groups, and at 17 days-old were (906±33, 844±153 and 1145±219) for G1, G2 and G3 respectively. There were significant differences (p
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تحضير بوليمر قابل لمتاكل الحيوي من حامض اللاكتيك باستخدام البكتريا المنتجة لحامض اللاكتيك == Preparation of Biodegradable Polymer of Lactic Acid using Lactic Acid Producing Bacteria

Author name: وصال عودة حسن الحلفي
Supervisor name: ميثم ايوب الحمداني | امال كاظم الاسدي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: English
University location: Basrah
First pages:

الاشكال السريرية والدلائل الحيوية للروماتيز مجهول السبب في اليافعين مقارنة بالروماتيز الرثوي في البالغين في عينة من مرضى عراقيين

Author name: حنان ياسين محسن
Supervisor name: منى تركي الموسوي | علي حسين ادحية
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

دور بعض المضادات الحياتية والكلايسين في انتاج اليوريز من بكتريا Helicobacter pylori المعزولة من المرضى المصابين بالقرح المعدية == Role of some antibiotics and glycine on urease production in Helicobacter pylori isolated from peptic ulcer patients

Author name: نادية عامر صادق النعيمي
Supervisor name: سوسن حسن عثمان | حيدر صباح الكواز
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

العلاقة بين جينات تكوين الغشاء الحيوي و انتاج البايوسيانين في بكتريا Pseudomonas aeruginosa المعزولة من مصادر سريرية == The relationship between biofilm formation and pyocyanin producing genes in Pseudomonas aeruginosa isolated from clinical sources

Author name: مها مخلف كاظم الشمري
Supervisor name: محمد فـــرج المـــرجاني
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

طفرات مجاور اللب وحفاز اساس اللب لفايروس التهاب الكبد ب وعلاقته بالاصابات المقاومين للعلاج في بعض المرضى العراقيين == Precore and Basal Core Promoter mutations of chronic Hepatitis B virus in relation to drug resistance chronic infections in some Iraqi patients

Author name: مصطفى جواد كاظم
Supervisor name: كاظم هاشم ياسين الاعرجي | عباس محمود احمد الجاف
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

التصنيع الحيوي لمادة اوكسيد التيتانيوم متناهية الصغر من عصيات حامض اللاكتيك وتاثيرها في بعض البكتريا المسببة لاصابات المجاري البولية المتكررة في عينة من المرضى العراقيين == Biosynthesis of titanium oxide nanoparticles by Lactobacillus spp. and their activity against some bacterial isolates associated with recurrent urinary tract infection in a sample of Iraqi patients

Author name: كوثر حكيم ابراهيم باجلان
Supervisor name: جيهان عبد الستار سلمان | فاطمة عبودي علي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

دراســة مناعيـة و جــزيـئـيـة لمستقبلات شبيـه التـول 4 , 2 في مـرضى مـتلازمـة تعفــن الدم البكتيــري == Immunological and Molecular Study of Toll - Like Receptor 2 and 4 in Patients with Bacterial Sepsis Syndrome

Author name: فرح قحطان يونس السامرائـي
Supervisor name: علي حسين علوان | نهاية حكمت زكي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تقييم العلاقة بين تكوين الغشاء الحياتي ونظام الذيفان ضد الذيفان النمط الثاني في بكتريا A.baumannii المقاومة للاميبنيم == Assessment correlation between biofilm formation and type II toxin - antitoxin system in imipenem - resistant Acinetobacter baumannii

Author name: فاطمة صبيح علي السعدي
Supervisor name: سوسن حسن عثمان | محمد فرج المرجاني
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

التحليل الجزيئي لبكتريا Staphylcoccus aureus المقاومة للمثيسيلين المعزولة محليا وعلاقتها مع جينات التحسس بالنصاب وبعض عوامل الضراوة == Molecular analysis of locally isolated methicillin resistant Staphylococcus aureus in correlation with quorum sensing genes and some virulence factors

Author name: علي جعفر سليم
Supervisor name: نزار ادور ناصر| منعم رضوان علي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

التصنيع الحيوي لجسيمات اوكسيد الزنك متناهية الصغر من بكتريا المعززات الحيوية وتاثيرها على الاصابات الجلدية البكتيرية == Biosynthesis of zinc oxide nanoparticles (ZnO NPs) by probiotics bacteria and their effect on bacterial skin infections

Author name: عفراء علي كاظم العسكري
Supervisor name: جيهان عبد الستار سلمان | عدوية جمعه حيدر
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

التحري الجزيئي لجينات مقاومة المضادات المفرطة واستشعار النصاب في بكتريا Klebsiella pneumoniae و Acinetobacter baumannii == Molecular detection of XDR and quorum sensing genes in local isolates of Acinetobacter baumannii and Klebsiella pneumoniae

Author name: روى عبد الرضا عزيز
Supervisor name: سوسن ساجد الجبوري
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تاثير بعض المواد النانوية على عوامل الضراوة المسيطر عليها بجينات تحسس الزحام في عزلات سريريه لبكتريا الزوائف الزنجارية == Effect of some nanomaterials on virulence factors controlled by quorum sensing genes in clinical isolates of Pseudomonas aeruginosa

Author name: رقية صبار سلمان الكبيسي
Supervisor name: سوزان سعدي حسين | منعم رضوان علي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

تشخيص فايروس شلل الاطفال (سابين) المعزول من الاطفال المصابين بالشلل الرخوي الحاد في العراق == Detection of Sabin (Poliovirus) in children with Acute Flaccid Paralysis in Iraq

Author name: رزاق هادي عيسى
Supervisor name: كاظم هاشم ياسين الاعرجي | اياد كاظم زغير
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
First pages:

اهمية جينات الانظيمات الناقلة للفيكوز والخمج البكتيري في متلازمة تكيس المبايض المتعدد == The significance of fucosyltransferases genes and bacterial infection in polycystic ovary syndrome

Author name: حنين اكرم حبيب
Supervisor name: خضر حسن علي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
University: Mustansiriyah University - College Of Science
Language: English
University location: Baghdad
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