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في مرضى ذات الرئة التباين الوراثي في جيني IL - 1? IL - 10 == polymorphism of IL - 10 and IL - 1? genes in Patients with Pneumonia

Author name: ايمان مبدر نايف عبد الحسن
Supervisor name: فريال جميل عبد عطية
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: English
University location: Babylon
First pages:

دراسة بعض الدلائل الفسلجية والكيموحيوية في الاشخاص المصابين بمتلازمة الايض في محافظة كربلاء == Study of Some Physiological and Biochemical Markers in Metabolic Syndrome Patients in Kerbala Province

Author name: حيدر بخيت عباس الكريطي
Supervisor name: ستار جاسم حتروش | فاضل جواد ال طعمة
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Karbala
First pages:

تطور ظهور صفة المقاومة في حشرة الذبابة المنزلية Musca domestica Linnaeus (1758) (Diptera : Muscidae) باستعمال منظمي النمو Trigard و Match == Development Occurrence of Resistance Feature in House flies Musca domestica Linnaeus (1758) (Diptera : Muscidae) Two Growth Match and Trigard

Author name: الاء سجاد صيهود حسين ا لخفاجي
Supervisor name: رافد عباس علي العيسى
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Karbala
First pages:

تاثير مستخلص نبات الحامول في السيطرة على داء السكري نوع 1 المستحث في ذكور الجرذان البيض == Effect of Cuscuta chinesis Lam. Extract in Controlling Induced Diabetes Mellitus Type - 1 in Albino Male Rats

Author name: فادیة حمید محمد علي السلطاني
Supervisor name: علي حمود محسن السعدي | ابتھال معز عبد المھدي الحسیني
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: English
University location: Babylon
First pages:

تاثير فيتامين E في تنشيط معالم النطف في الزجاج لمرضى العقم المصابين بقلة النطف ووهنها == Effect of Vitamin E on in Vitro Sperm Activation of Oligospermic and Asthenospermic Infertile Patients

Author name: ضرغام فالح حسن الشمرتي
Supervisor name: صباح ناصر العلوجي | يحيى كاظم السلطاني
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت هذه الدراسة على 217 عينة من عينات السائل المنوي التي تعود الى 207 من المرضى الذين يعانون من قلة النطف Oligospermia ، ووهن النطف Asthenospermia ، وابيضاض المني Leukospermia من مراجعي المختبر الاستشاري لكلية الطب في جامعة الكوفة ، للمدة مابين تشرين الاول 2007 وايار 2008. وتم في هذه الدراسة تنشيط النطف البشرية لاولئك المرضى في الزجاج in vitro باستعمال تقنيتين مختلفتين هما : تقنية السباحة للاعلى وتقنية الخلط technique Mixing ولمدة تحضين مقدارها ثلاثين دقيقة في المستنبت ايرل للمرة الاولى ومستنبت ايرل + فيتامين E للمرة الثانية ، ومن ثم بيان تاثير فيتامين E في معالم النطف ، وكذلك بيان تاثير الالتهابات في معالم النطف قبل العلاج وبعده. اظهرت نتائج علاج المرضى الذين يعانون من ابيضاض المني حصول انخفاض معنوي (0.05 >P) في تركيز خلايا الدم البيض والخلايا البلعمية ، في حين كان الانخفاض غير معنوي (0.05 <P) في زمن الاماعة قياسا بقيمتها قبل العلاج فضلا عن حصول تحسن معنوي (0.05 >P) في تركيز النطف ، والنسبة المئوية للنطف المتحركة ، ومعامل حركة النطف ، ودرجة نشاط النطف قياسا بقيمتها قبل العلاج وكذلك عند اجراء عملية التنشيط قبل العلاج وبعده. اشارت نتائج تنشيط نطف المرضى الذين يعانون من قلة النطف (الحاد والمعتدل) ووهن النطف (الحاد والمعتدل) باستعمال تقنية السباحة للاعلى وتقنية الخلط (مستنبت ايرل لوحده ومستنبت ايرل+ فيتامين E) حصول تحسن معنوي (0.05 >P) في النسبة المئوية للنطف المتحركة ودرجة نشاط النطف ومعامل حركة النطف عند المقارنة بقيمتها قبل التنشيط. اشارت نتائج تنشيط نطف المرضى الذين يعانون من قلة النطف (الحاد والمعتدل) ووهن النطف (الحاد والمعتدل) باستعمال تقنية السباحة للاعلى (مستنبت ايرل لوحده ومستنبت ايرل+ فيتامين E) حصول انخفاض معنوي (0.05 >P) في تركيز النطف وتركيزخلايا الدم البيض عند المقارنة بقيمتها قبل التنشيط ، في حين لم تكن هناك فروقات معنوية في المعلمين المذكورين عند استعمال تقنية الخلط . اشارت نتائج تنشيط نطف المرضى الذين يعانون من قلة النطف (الحاد والمعتدل) ووهن النطف (الحاد والمعتدل) باستعمال تقنية السباحة للاعلى (مستنبت ايرل لوحده ومستنبت ايرل+ فيتامين E) حصول تحسن معنوي (0.05 >P) في النسبة المئوية للنطف السوية المقارنة بقيمتها قبل التنشيط ، في حين لم تكن الفروق معنوية في النسبة المذكورة عند استعمال تقنية الخلط . كما لوحظ حدوث تحسن معنوي (0.05 >P) في النسبة المئوية للنطف المتحركة ، ودرجة نشاط النطف ، ومعامل حركة النطف في عملية التنشيط في تقنيتي السباحة للاعلى والخلط عند استعمال مستنبت ايرل ممزوجا مع فيتامين E عند مقارنتها بتلك التي استعمال فيها مستنبت ايرل لوحده ، في حين كان هناك تحسن معنوي (0.05 >P) في تركيز النطف في تقنية السباحة للاعلى عند استعمال مستنبت ايرل مع فيتامين E عند مقارنتها بتركيز النطف عند استعمال مستنبت ايرل لوحده. يستنتج من هذه الدراسة ان العمل على تنشيط النطف البشرية بعد علاج الالتهابات يعطي نتائج افضل بكثير من التنشيط قبل العلاج ، لما لخلايا الدم البيض والخلايا البلعمية من تاثير مهم في معالم النطف . كذلك فان اضافة فيتامين E الى مستنبت ايرل اثبت من الدراسة كفاءة عالية في الحصول على قيم جيدة لمعالم النطف سواء عند تنشيط نطف مرضى العقم المصابين بقلة النطف (الحاد والمعتدل) او نطف المصابين بوهن النطف (الحاد والمعتدل). | This Study was performed on 217 seminal fluid samples belongs to 207 patients suffering from Oligospermia , Asthenospermia and Leukospermia at the consulting Laboratory of College of Medicine Kufa University during the period between October, 2007 and May, 2008. The objective of the persent study is to activation patient sperms quality in vitro by using two different techniques which are the swim - up technique and mixing technique for 30 minute of incubation in Earl’s medium and Earl’s medium + vitamin E to findout the effect of vitamin E on the sperms parameter. Althow, also the effects of infection on the sperm parameter before and after treatment ware evaluated also. . The results showed that the treatment of patient suffering from leukospermia showed revealed significant decrease (P<0.05) in the leukocyte and phagocyte concentration. However, the decrease was insignificant at the time of liquification in comparison with thar used befor treatment . Also a significant improvement (P<0.05) was found in sperm concentration, sperm motility percent, sperm motility index and sperm grade activity in comparison to that resulted befor treatment, as well as that done at the process of activation before and after the treatment. . The sperm activation of patients suffering from Oligospermia (severe and moderate) and Asthenospermia(severe and moderate) by using the swim - up technique and mixing technique (Earl’s media and Earl’s media + vitamin E) showed that there was a significant improvement (P<0.05) in the sperm motility percent, sperm grade activity, sperm motility index in comparison to its result befor activation. The result of sperm patients suffering from Oligospermia (severe and moderate) and Ashenospermia (severe and moderate) by using the swim - up technique (Earl’s media and Earl’s media + vitamin E) showed that there was a significant decrease (P<0.05) in sperm concentration and leukocyte concentration in comparison to that used before activation. However, the results were not significant in both mentioned parameter when mixing technique was used. The result of sperm activation of patients suffering from Oligospermia (severe and moderate) and Asthenospermia(severe and moderate) by using the swim - up technique (Earl’s media and Earl’s media + vitamin E) showed that there was a significant improvement (P<0.05) in the normal sperm morphology percent in comparison to its result befor activation. However, the sperm percent was not significant when mixing technique was used. Also, a significant improvement was noticed(P<0.05) in the sperm motility percent, sperm grade activity and sperm motility index by using ng both techniques swim - up and mixing when Earl’s media was mixed with vitamin E in comparison to the process of activation in both techniques when only Earl’s media was used. However, there was a significant improvement (P<0.05) in sperm concentration swim - up technique by using Earl’s media and vitamin E when compared to that in which only Earl’s media was used . It was concluded that using a human sperm activation after the treatment of infection and inflammations can give a better results than that when used before the treatment as the presence of leukocytes and phagocytes considered important in sperm parameters . Also, it was found that the addition of vitamin E to Earl’s media improve the sperm parameters and their activation in infertile patients suffering from Oligospermia (severe and moderate) and Asthenospermia (severe and moderate)

ارتباط التغايرات الوراثية لجينات نظام الدوبامين مع السلوك الاجرامي العنيف للسجناء العراقيين == Association of Dopaminergic System Genes Polymorphisms with Violent Criminal Behavior in Iraqi Prisoners

Author name: سهاد رضا متعب الطائي
Supervisor name: محمد عبد الله جبر جاسم
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: The violent criminal behavior is emerged from the interaction between several genetics and environmental factors. The present study reflect the role of polymorphisms in dopaminergic system genes (drd2 ,comt ,dat1 ,drd4, and maoa) on violent criminal behavior in Iraqi prisoners.This research was a case - control study , blood samples were collected from 200 prisoners (case group) who convicted with terrorism (150 sample), murder (30 sample) and drug trading (20 sample) Issues selected from Al - Hilla reformist central prison for men and women in Babylon city and from position and deporting division in Karbala .This sample included (160 male and 40 female). Additionally, 100 sample were collected as control group included (54 male and 46 female).DNA was extracted and the polymorphisms analysis achieved by Restriction Fragment Length Polymorphism (RFLP - PCR) for drd2 and comt genes ,while the dat1 ,drd4 and maoa polymorphisms was analyzed by Polymerase Chain Reaction (PCR).All polymorphisms (except drd4) were subjected to PCR - sequencing for confirming the presence of polymorphisms .The results showed that the percentage of the case group was 34.5 % with an age ranged between 20 - 39 years ,most of them were male 80% ,72.5% were married ,77.5% were from rural area ,84% were self - employed and 41.5% were illiterate .Genetic analysis of drd2 TaqIA polymorphism demonstrated that a significant increase in the genotype frequency of A1/A1 was observed in case group (p = 0.03; OR= 4.93 ; 95% CI=1.08 - 22.5) and the A1 allele frequency was significantly associated with criminal behavior (p=0.03 ;OR=1.51; 95% CI=1.04 - 2.21).The comt (Val 158 Met) polymorphism revealed that the homo - mutant genotype A/A had significant higher risk of criminal behavior (p= 0.001 ;OR= 3.98 ; 95% CI= 1.7 - 9.3) when compared with control group. and the A allele(met allele) frequency was significantly associated with case group (p=0.003; OR= 1.68; 95%CI= 1.19 - 2.37).On the other hand , the analysis of VNTR polymorphism in dat1 gene showed non - significant difference in 9/9 genotype and 9R allele frequency between the study groups (p>0.05).The VNTR polymorphism analysis for drd4 gene revealed that the criminal individual who carried 7/4 genotype was significant higher than in control group (p=0.03;OR= 2.6, 95%CI= 1.09 - 6.17), and the 7R allele frequency was significantly associated with case group (p=0.04;OR=2.06; 95%CI= 1.04 - 4.1).Finally, the maoa VNTR polymorphism was analyzed in male and female separately due to the gene location on X - chromosome .The results demonstrated that there was non - significant difference observed between all genotypes and alleles frequencies between case and control male (p> 0.05),whereas in female a significant association in 3.5/3.5 and 5.5/4.5 genotypes (p<0.01) ,additionally the 3.5 R alleles was associated with higher risk of violent criminal behavior in female (p= 0.004;OR=2.62; 95%CI=1.36 - 5.05).These results suggested that the drd2 ,comt ,drd4 and maoa in female polymorphism might increase the susceptibility of individual to engage in to crimes in the presence of certain environment risk factors.

دراسة جزيئية ومناعية لفايروس النورو والفايروس الغدي نمط 40 و41 في الاطفال المصابين بالاسهال == Molecular and Immunological Study of Norovirus and Adenovirus types 40 and 41 in Diarrheic Children

Author name: رمله احمد محمد حسن العميدي
Supervisor name: مهدي حسين محيل العمار
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: هدفت هذه الدراسة الى التحري عن فايروسي النورو والفايروس الغدي نمط 40 / 41 في الاطفال المصابين بالاسهال واجراء تحليل لمعدل الاصابة وطبيعتها ودراسة الطبيعة الجزيئية للفايروسين وكذلك التحري عن قدرة الفايروسين على حث الجهاز المناعي وتحديد بعض الحركيات الخلويه عند الاطفال المصابين بالفايروسين.بالاضافة الى دراسة بعض ملامح الاصابه مثل تغاير الفصول والعمر وتحديد افضل الطرق لتشخيص الفايروسين. اجريت الدراسة الحالية في محافظة النجف الاشرف /مستشفى الزهراء التعليمي للولادة والاطفال خلال الفترة من كانون الثاني2014 الى كانون الاول 2014 والتي تضمنت 314 عينه من البراز والدم من الاطفال المصابين بالاسهال والذين تتراوح اعمارهم بين شهر الى خمسة سنوات.حيث اعتمد التحري في عينات البراز استخدام اختبار الكروماتوغرافيا المناعي السريع للكشف عن المستضدات الفايروسية للفايروسين النورو والغدي نمط 40 / 41.كما تم تاكيد العينات الموجبة بواسطة تقنية PCR Real Time لفايروس النورو تقنية PCR للفايروسالغدي نمط40 / 41،والتي اظهرت فيها النتائج بان معدل الاصابة كان 11.7% لفايروس النوروو8.9% للفايروس الغدينمط 40 / 41.كذلك تم فحص العينات الموجبة بواسطة المجهر الالكتروني والتي بينت مظهر للفايروسين في عينات البراز . وتشير الدراسة الى ان التقنيات الجزيئية المستخدمة في فحص العينات هي الاكثر كفاءة للتشخيص الروتيني للفايروسين مقارنة مع المجهر الالكتروني لتشخيص عينات الاسهال.وطبقا للفئات العمرية ،اظهرت النتائج ان الفئة العمرية 13 - 24 شهر سجلت ارتفاع عالي للاصابة بالفايروسين النورو والغدينمط 40 / 41.الحالات الايجابية كانت بين الاطفال الذين يتغذون على الحليب الجاف الاصطناعي (31 حاله) مقارنة مع الرضاعة الطبيعية من الام (14 حاله).اما نتائج الاعراض السريرية في الاطفال المصابين بالفايروسين فاظهرت ان كل الحالات تبين وجود اسهال من النوع المائي المخاطي وبنسبة 100% يليها الالم البطن بنسبة 94.5% و82.1 %،التقيؤ بنسبة 81 % و78.5 % واخيرا الحمى بنسبة 78.3% و71.1 % لكلا من النورو والغدي نمط 40 / 41 على التوالي. كما كشفت الدراسة الحالية بان معدل الاصابة المرتفع لفايروس النوروكان من شهر كانون الثاني الى اذار والتي كانت بنسبة 81%من الحالات الموجبة ، اما بالنسبة لفايروسالغدينمط 40 / 41كان معدل الاصابة المرتفع من شهر كانون الثاني الى نيسان والتي كانت بنسبة 89.2% من الحالات الموجبة. كما اظهرت النتائج وجود ارتفاع كبيرفيالحركيات الخلويه (السايتوكينات) لمصل الاطفال المصابين مقارنة مع السيطرة. وفقا للفئات العمرية ،اظهرت الفئة( 49 - 60) شهر ارتفاع عالي في مستويات الانترفيرون كاما ، الانترلوكين - 6((IL - 6 والانترلوكين - 12 (12 IL - (حيث كانت للفايروس النورو(90.8, 56.7, 84.3) جزء من الغرام /مل على التوالي،بينما كانت لفايروسالغدي(106.5, 59, 87.5)جزء من الغرام /ملعلى التوالي مقارنة مع مجموعة السيطرة. | Norovirus and adenovirus types (40 and 41) are the important causes of gastroenteritis infections in children worldwide. The present study was designed to determine the incidence and nature of infection of norovirus and adenovirus types (40 and 41) at molecular and immunological levels, as well as some features such as seasonal variation , age distributions and detected the most effective and suitable method for diagnosis of the two viruses . The study was carried out in Al - Najaf province, in AL - Zahra teaching hospital for maternity and children, during the period from January, 2014 to December, 2014. Stool and blood samples were collected from 314 children with diarrhea, their age range (from 1months to 5 years). Immunochromatographic assay for norovirus and adenovirus used to detect their antigens in stool samples, the positive results were confirmed by reverse transcriptase real time PCR technique for norovirus and polymerase chain reaction technique for adenovirus types (40 and 41), which showed incidence of 11.7% and 8.9% for norovirus and adenovirus, respectively. Transmission electron microscopy examination gave clear positive norovirus and adenovirus types (40 and 41) appearance in diarrheal stool samples .The study indicates that molecular methods are most suitable and sufficient tests for routine diagnosis of norovirus and adenovirus types (40 and 41) as compared to transmission electron microscopy analysis for diarrheal samples. According to age groups, the results showed that the majority of infections with norovirus and adenovirus were recorded among age group (13 - 24) months. The positive cases were highly among infants fed with bottle fed (31 cases) compared with infants fed with breast fed (14 cases). Regarding the clinical features of children with viral diarrheal infections , the results showed that all of the cases of both viruses complained of watery or mucosal consistence stool (100%) followed by abdominal pain (94.5%) and (82.1%), vomiting (81%) and (78.5%) , and fever (78.3%) and (71.4%) for norovirus and adenovirus positive children respectively. The present study also revealed that a higher incidence of norovirus was detected from January - March 2014, when it was found in 81% (30/37) of the positive cases, whereas the incidence of adenovirus was higher from January - April 2014, during which it was found in 89.2% (25/28) of the positive cases. The current study for cytokines profile detection showed that significant elevation in patients with norovirus and adenovirus than control. The age groups 49 - 60 months have shown a high level in cytokines Interferon gamma, Interleukin - 6 and Interleukin - 12 with concentration were (84.3, 56.7, 90.8) pg./ml , respectively for norovirus while adenovirus were (87.5, 59, 106.5) pg./ml , respectively as compared with control group . Conclusion of the study shows incidence norovirus genotypeII (GII) and adenovirus types (40 and 41) by rapid chromatographic test from infected children in Al - Najaf province. The study indicates that molecular techniques are most suitable and sufficient tests alternatively for transmission electron microscopy when there is good facility and both can replaced by rapid chromatographic test . Interferon gamma, Interleukin - 6 and Interleukin - 12 were increased in all patients with norovirus and adenovirus infections and showed high level in age group (49 - 60) months

دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية : دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية والوراثية عند العاملين في معمل البتروكيمياويات في محافظة البصرة والوراثية عند العاملين في معمل البتروكيمي == The Study of the effects of previous Ethylene Exposure on Some Physiological and Genetic Parameters in Workers of Petrochemical Plant In Basrah province

Author name: هبة ثاقب يسر
Supervisor name: سامي جبر المالكي | فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was conducted to investigate the genetic and physiological effects of some industrial chemicals on workers of the Petrochemicals Industrial Factory, in Basra Province , as it has been collecting 100 blood samples during the month of January and February of 2011 from each of the exposure in (ethylene section) and the control group of (employees of the University of Basra). and then the samples were divided on the basis of each of the years of occupational exposure to more and less than 15 years , age group to the larger and smaller than 50 years, smoking habits.The results showed that there was a significant decrease in the haemoglobin concentration HB, PCV, RDW and MCV in the more than15 years exposure group in comparison with the control group, while, no significance was detected in the RBC, MCH, MCV ,RDW,MCH and MCHC in less than15 years exposure group . Whereas, a significant decrease in the PCV for the same group was observed.White blood cells parameters’ results showed also variations in their values. In general, WBC did not change significantly in both groups of workers, while, the proportion of the lymphocyte and the of monocyte declined significantly in the more than 15 year of occupational exposure group, in contrast, the proportion of granulated cells increased markedly in the same group. The results of group of less than 15 years exposure revealed that the proportion of monocyte dropped significantly. Conversely, there was a significant increase in the proportion of granulated cells while the proportion of the lymphocyte was not significant . Platelets’ number and average of platelets’ volume decreased significantly in both occupational groupsTurning to the biochemical tests, the results showed significant increase in both liver enzymes ALT ,ASTand bili as well as this increase was found in urea, uric acid and glucose values in both occupational exposure groups. Contrariwise, the ALP creatinine and cholesterol did not reveal significant changes in both groups that in comparison with control group. The total protein decreased significantly in the group of more than 15 year of occupational exposure.Testosterone level revealed a significant decline in both occupational groups, while the Follicle stimulating hormone showed a significant increase in the occupational group of less than 15 year and no difference was observed in another occupational group while LH hormone level was not significant.In case of the genetic effects of the ethylene compounds, DNA was extracted for control and occupational groups and amplification was performed for GSTM1, GSTT1 and Albumin by using PCR technique. The results showed that there was no significant effect of both occupational periods on the GSTM1 gene, while GSTT1 gene was significantly in the workers samples in group more than 15 year of exposure, which was 2.43 times more than the group of less than 15 year of exposure.As regards the impact of exposure to ethylene and loss of genes GSTM1 and GSTT1 showed the results of our study, significant increase in the loss of gene GSTM1 amounted to more than five - fold (5.1) among workers exposed compared to a set of control while the proportion of loss of gene GSTT1 by more than three - fold (3. 1) compared to the group control has been a loss ratio of the two genes together more than eight - fold (8.5) for workers exposed group compared to the control.The light smoke did not reveal a significant effect on the null of GSTM1 and GSTT1 genes that in comparison with the control group, while the study found a significant effect of smoking on gene GSTM1, causing the null of much more than doubled (2.2) times compared with the control group (non - smokers) and the significant effect of smoking on null GSTT1 gene loss as increased visits by the text (1.5) compared with non - smokers also appeared that excessive smoking has an effect on the GSTT1 gene causing loss by seven - fold (7.08) once compared to non - smokers.The effect of the age group (less and more than 50 years old) of the workers on the null of both GSTM1 and GSTT1 was also examined. GSTM1 gene was null six times in both age groups of workers more than the control samples. While the GSTT1 was null in age group less than 50 years old three times more than the control group. As well as this absence was two times in the age group more than 50 years old in comparison with control group.It could be concluded from the present study that the exposure of workers to ethylene and its metabolic impact and clearly on blood standards as well as caused a high level of enzymes AST, ALT and bilirubin and high blood sugar levels and lower total protein and high level of urea and uric acid level as well as the low level of the hormone testosterone as it turns out that occupational exposure to ethylene and metabolic outcomes and length of exposure and smoking for workers exposed role in influencing the genes of detoxification, causing null in each of the gene GSTT1 and GSTM1.

تاثير بعض المعاملات في زيادة تحمل نبات الموز (Musa acuminata L.) الصنف الهندي للشدين الملحي والمائي باستعمال تقانة زراعة الانسجة النباتية == Effect of some treatments on increasing salinity and water stress on Musa acuminata L. (Hindi cultivar) using plant Tissue Culture techniques

Author name: مريم جاسم محمد
Supervisor name: حسين خلف زاير | عباس مهدي جاسم
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية في المختبر التقني لزراعة الانسجة النباتية التابع للقطاع الخاص الواقع في منطقة الفيحاء / قضاء شط العرب/ محافظة البصرة للفترة من 2012/12/20 ولغاية 2014/12/20 على نبات الموز الصنف الهندي قصير الساق Musa acuminata L. بهدف وضع برنامج للاكثار الدقيق ومدى تحمل النبيتات للشد الملحي باستعمال كلوريد الصوديوم والشد المائي باستعمال مركب الكلايكول متعدد الاثيلين (PEG)Polyethelen glycol ومعرفة تاثير معوق النمو الباكلوبيوترازول (PBZ)Paclobutrazol في تضاعف الافرع الخضرية وفي اقلمة النبيتات وتاثير الهرمون النباتي حامض الابسيسك (ABA)Abscisic acid في تحسين التحمل للشدين الملحي والمائي. استعمل انصاف من القمم المرستيمية النامية Shoot tips كاجزاء نباتيةExplants على وسط MS مضافا اليه 5ملغم.لتر1 - من السايتوكاينين (BA)Benzyl adenine لتحفيز تضاعف الافرع الخضرية ومعاملتها بتراكيز مختلفة من ملح كلوريد الصوديوم وPEG وPBZ وABA وتم حساب عدد الافرع الخضرية الناتجة وقطرها وارتفاعها وعدد الجذور واطوالها ومتابعة نموها ثم اجراء عملية الزراعة الثانوية لعدة مرات ثم اقلمتها ونقلها الى البيت البلاستيكي.ويمكن تلخيص النتائج التي تم الحصول عليها بما يلي : 1 - اضافة ملح كلوريد الصوديوم بالتراكيز 0 او 20او50 او80 او120 ملي مولر الى الوسط MS اثر في تضاعف الافرع بنسب متفاوتة , وادى الى زيادة معنوية في المدة اللازمة لظهور الافرع الخضرية وتقليل عددها بزيادة تركيز الملح .2 - اضافة معوق النمو PBZ بالتراكيز 0 او0.5 او 1 او1.5 او2 ملغم.لتر1 - بوجود السايتوكاينين BA بالتركيز 5ملغم.لتر1 - ادى الى تقليل المدة اللازمة لبدء ظهور الافرع الخضرية وزيادة عددها وخفض ارتفاع النبات وعدد الجذور بزيادة تركيز PBZ.3 - تفوقت المعاملة الاولية بـPEG بالتركيز 1% معنويا على التركيز 0.5% في عدد الافرع الخضرية وفي عدد الجذور وطولها وزيادة في ارتفاع النبات وقطره .4 - اضافةABA الى الوسط الغذائي بالتركيزين 0.1 او0.3 ملغم.لتر1 - ادت الى تقليل عدد الجذور واطوالها وارتفاع النبات وقطره وتيبس الاوراق واصفرارها مقارنة مع معاملة السيطرة بينما تفوقت معاملة التداخل مع تراكيز كلوريد الصوديوم المدروسة مع المعاملتين 20و50 ملي مولر من كلوريد الصوديوم تفوقا معنويا على المعاملتين 80 و120 ملي مولر في زيادة عدد الجذور واطوالها وطول النبات وقطره . 5 - سببت اضافة كلوريد الصوديوم الى الوسط الغذائي زيادة معنوية في تركيزي ايوني الصوديوم والكلورايد وهبوط معنوي في تركيز ايون البوتاسيوم ونسبة البوتاسيوم /الصوديوم بينما ادت اضافة PBZ وPEG وABA الى انخفاض معنوي في ايون الكلورايد وزيادة معنوية في نسبة البوتاسيوم /الصوديوم .6 - سببت المعاملة بملح كلوريد الصوديوم زيادة معنوية في محتوى الاوراق من البرولين بزيادة تركيز الملح .كما ان تداخل تراكيز ملح كلوريد الصوديوم مع PBZ وPEG وABAقد سجلت زيادة معنوية في محتوى البرولين بزيادة تركيز الملح . 7 - ادت المعاملة بملح كلوريد الصوديوم عند التركيزين 20و50 ملي مولر زيادة محتوى الاوراق من الكربوهيدرات الذائبة الكلية في حين ادت المعاملة بالتركيزين و80 و120 ملي مولر الى هبوطها.اما بالنسبة لتداخل تراكيز ملح كلوريد الصوديوم مع تراكيز PBZ وPEG وABA فقد تفوقت معنويا في محتوى الاوراق من الكربوهيدرات عند جميع التراكيز مقارنة مع معاملة السيطرة .8 - اوضحت النتائج ان محتوى الاوراق من الكلوروفيل الكلي قد انخفض معنويا عند تركيزي80 او120 ملي مولر كلوريد الصوديوم .كما ان المعاملة بـ PBZ وPEG وABA ادت الى زيادة محتوى الكلوروفيل الكلي عند جميع التراكيز .9 - اقلمت النبيتات الناتجة من الافرع الخضرية المزروعة في الوسط الغذائي المزود بتراكيز ملح كلوريد الصوديوم فقط ومن تداخل كلوريد الصوديوم مع تراكيز PBZ وPEG وABA وقيس ارتفاع النبات وقطره وقورنت مع معاملة السيطرة .10 - ادت المعاملة بتراكيز PBZ وPEG وABA المتداخلة مع تركيزي ملح كلوريد الصوديوم 20 و50 ملي مولر الى خفض معدل النسبة المئوية للرطوبة المفقودة وزيادة النسبة المئوية للنباتات المؤقلمة.11 - اضافة كلوريد الصوديوم الى الوسط الغذائي ادى الى حدوث تغيرات في عملية التعبير الجيني وظهور اختلافات في عدد ومواقع وكثافة الحزم البروتينية .12 - التداخل بين كلوريد الصوديوم والمواد المضافة PBZ وPEG وABA ادت الى اختفاء بعض الحزم وظهور حزم اخرى في مواقع مختلفة خصوصا الحزمتين 3و 4 . | This study was conducted in the laboratory of the technical plant tissue culture in Fayhaa / Shatt Al - Arab District / province of Basra for the period from 12/20/2012 till 20/12/2014 at Dwarf banana cv. Indian Musa acuminata L. In order to develop a program for micropropagation and were to investigate salt stress tolerance through the effect of NaCl, and water stress through Polyethelen glycol(PEG) and knowledge of the growth retardant of Paclobutrazol (PBZ) in multiplication of the branches vegetative and acclimatization and the effect of Abscisic acid(ABA) on improving salt and water stress tolerance. Halves of shoot tips were used explants which were cultured on MS medium supplied 5 m.l - 1 of Benzyl adenine (BA) to stimulate the multiplication of the branches vegetative and treated with different concentrations of sodium chloride and PEG and salt PBZ and ABA were calculating the number of branches vegetative resulting in diameter and height and number roots and length of follow - up and growth of agriculture and then conduct subculture for several times and then acclimatized and transferred to the plastic house.The results of this study can be summarized as follows : 1 - The addition of 0 or 20 or 50 or 80 or 120 mM of sodium chloride to the MS medium affected the multiplication of the branches vegetative in various percentages. This, in turn, resulted in a signficant increase in the required time to initiate the adventitious buds and lowered their number. There was also a significant difference in the percentage of increasing the numbers of those branches by increasing the salt concentrations. 2 - The addition of the concentrations 0 or 0.5 or 1 or 1.5 and 2 mg.l - 1 of growth retardant PBZ together with 5 mg.l - 1 BA resulted in lowering the required time for initiating the adventitious buds, increasing their number, decreased the number of roots and height of stem by increasing the concentration of PBZ.3 - The initial treatment by 1% PEG significantly increased than the concentration 0.5%. This increase included the number of the adventitious buds, number and length of roots, as well as height and diameter of stem.4 - The addition of 0.1 and 0.3 mg.l - 1 ABA to the nutrient medium led to lower th number and length of roots, stem height and diameter. The leaves became dry and yellow when compared to the control treatment. As far as the interactions between the sodium chloride concentrations in question are concerned, the two concentrations 20 and 50 mM showed a more significant difference than the other concentrations 80 and 120 mM in the number and length of roots, and stem length and height.5 - The addition of the sodium chloride to the nutrient medium resulted in a significat increase in the concentrations of sodium chloride ions and a significant decrease of potassium/ sodium percentage. The addition of PBZ, PEG, and ABA resulted in a significant decrease in chloride ion and a significant increase in potassium/ sodium percentage.6 - The sodium chloride treatment caused a significant increase in the leave content of Proline by incresing the salt. The interaction between sodium chloride salt concentrations and PBZ, PEG, and ABA showed a significant increase in the proline content by increasing salt concentration.7 - The treatment by the two concentrations 20 and 50 mM sodium chloride resulted in increasing the leave content of the total soluble carbohydrates, whereas the treatment by the two concentrations 80 and 120 Mm lowered the leave content. Considering the interaction between sodium chloride and PBZ, PEG and ABA, the interaction showed a significant increase in the leave content of carbohydrates at all concentrations compared to the control treatment.8 - The results showed that the leave content of chlorophyl increased significantly at the two concentrations 20 and 50 mM sodium chloride. Also, the treatment by PBZ, PEG, and ABA showed an increase in the chlorophyl content at all concentrations.9 - The resulted plantlets were acclimatized by sodium chloride salt concentrations and by the interaction of Sodium Chloride and PBZ, PEG, and ABA. The plant height and stem diameter were measured and compared to the control treatment.10 - The treatment by PBZ, PEG, and ABA concentrations that are interacted with Sodium Chloride salt concentrations at 20 and 50 mM led to lower the average percentage of lost moisture and increase the percentage of the success of acclimatization. 11 - Add Sodium chloride to the media has led to changes in the process of gene expression and the emergence of differences in the number and location and intensity of the protein packets.12 - The interaction between the sodium chloride and additives PBZ and PEG and ABA led to the disappearance of some packages and the emergence of other packages in different locations, especially packages 3 and 4.

دراسة مقارنة لتاثير هرمون النمو في عضلات وCyprinus مناسل سمكتي الكارب الاعتيادي والبلطي احمر البطن carpio (L. 1758) Tilapia zillii (Gervais, 1848 == A Comparative Study to the Effect of Growth Hormone on Muscles and Gonads of Carp Cyprinus carpio (L. 1758) and Tilapia Tilapia zillii (Gervais, 1848)

Author name: سعد محمد صالح عبد الصمد
Supervisor name: عقيل جميل منصور | عبد الكريم طاهر يسر
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study, the effect of human growth hormone on the muscles and gonads of common carp Cyprinus carpio (L. 1758) and red belly tilapia Tilapia zillii (Gervais, 1848) was studied by : - 1 - Studying the effect of human growth hormone on the growth of the two studied species by intraperitoneal (IP) injection of three replicates of control with 120 μg of normal saline / g Body weight and two doses (low and high) with 60 and 120 μg of human growth hormone / g Body weight respectively for both species, then changes were followed up in each treatment after every two weeks of injection by : - a - Pursue histological changes in the muscular tissue by using Cell Profiler software for the first time in Iraq. b - Pursue changes in chemical components ratios of body muscles.c - Measuring of specific growth rate and length - weight relationship for fishes.2 - Studying of human growth hormone effect on the gonads of fishes (adult males and females) with 60 males for each species and a same number of females, all of them injected either with low or high dose with 60 or 120 μg of human growth hormone / g Body weight respectively for both species or with 120 μg of normal saline / g Body weight in fishes of control. This experiment had achieved in a month.3 - Knowing the direct effect of human growth hormone on the follicles by implementing an in vitro experiment with the follicles of carp which implanted in basic salt solution (BBS) then growth hormone was added to the test tubes with two concentrations 5 μg/ ml and 10 μg/ ml, the control follicles were implanted in normal saline. The results of the study revealed that the maximum rate of percentages of the muscle fibers with area less than 314 μm2 for region R1(Dorsal side) was 88.29 % in carp in high dose treatment after the eighth week of injection, while it was 88.00 % for tilapia in high dose treatment after the eighth week of injection, too.In the region R2 (in tail) the maximum rate of percentages of muscle fibers with area less than 314 μm2 was recorded after the eighth week from the beginning of injection was 73.3553 % for carp in high dose treatment and 85.935% for tilapia in high dose treatment.The results showed that 1.00984 and 1.000074 were the highest and the lowest values of condition factor for carp respectively. For tilapia those were 1.009993 and 1.000151 the highest and the lowest values of it respectively.The rate of specific growth rate for length was 0.3616 and 0.44866 cm/day% for carp and tilapia respectively. While the specific growth rates for weight were higher than the comparable one for length and were 1.3489 g/day% for carp and 1.6085 g/day% for tilapia.The results exhibited that the rates of protein percentages for both species in the all treatments in general tend to be higher with progressing of experimental time. Muscle water content percentage rate varied with difference of dose and experimental time. The maximum rate of fat percentage in both species was in control treatment after two weeks of hormone administration.The highest rate of carp male Gonadosomatic index was 6.55% in high dose treatment after the third week of injection, as for tilapia males was 6.103% in low dose treatment after the third week of injection, while the lowest value for this index was recorded after the first week of injection in high dose treatment which as 2.81 and 2.89% for carp and tilapia respectively. For females the highest rate of Gonadosomatic index was in high dose treatment after the third week of injection for both species which was 11.001 and 6.0624% for carp and tilapia respectively. As for the lowest rate of carp female Gonadosomatic index was recorded in control treatment after the first week of injection it was 5.54%, while the lowest rate of this index for tilapia females was 2.99% in low dose treatment after the first week of injection.In the experiment of direct effect of this hormone on the follicles of carp it was found that the highest value of polarization index was 4.12 which recorded in high dose treatment after 48 hours of incubation while it reached the lowest value which was 3.031 in high dose treatment after 24 hours ofincubation.

دراسة تصنيفية وبيئية لحشرات نصفية الاجنحة المائية وشبة المائية Heteroptera : Hemiptera في محافظة البصرة == Taxonomical And Ecological Study of Aquatic And Semiaquatic Insects(Heteroptera : Hemiptera) In Basrah Provience

Author name: اسماء عبد الزهرة سبع العيداني
Supervisor name: ضياء خليف كريم
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: Present studies dealing with the taxonomical and ecological studies of the aquatic Hemiptera : Heteroptera in eight stations in Basrah provience ,which are : Al - Medina , Al - Qurna , Basrah center ,Shat Al - Arab , Al - Zubair , Abu Al - Khasib , Al - Faw and Sullein Marsh, collection was done twise monthly during the period from April 2012 to March 2013 .Results showed the presence of nine species belonging to aquatic Hemiptera , three belonging the group Gerromorpha and six the group Nepomorpha,as follows : Group : Gerromorpha(Popov,1971)Family : Gerridae ( Leach , 1815)1 - Aquarius nebularis(Schellenberg,1800)Family : Mesoveliidae(Douglast and Scott , 1867 )2 - Mesovelia vettigera( Horvath , 1895 ) ( apterous )M .vettigera( Horvath , 1895 ) ( macropterous )Family : Macroveliidae3 - Macrovelia hornii(Uhler , 1872 )Grouop : Nepomorpha(Popov,1968)Family : Nepidae ( Latreille , 1802 )4 - Nepa cinerea( Linnaeus , 1758 )Family : Corixidae ( Leach,1815 )Subfamily : Cymatianiae ( Walton , 1940 )5 - Cymatia bonsdorffii(Sahlberg , 1819 )Subfamily : Corixinae ( Leach , 1815 )6 - Sigara(Vermicorixa) latelaris ( Leach , 1815 )Family : Belostomatidae ( Leach , 1815 )7 - Lethocerus fakir(Mayr , 1852)Family : Notonectidae ( Latreille , 1802 )8 - Anisops sardeus(Herrich - Schaeffer , 1849)AbstractBFamily : Pleidae ( Fieber , 1851 )9 - Plea leachi(McGregor and Kirdaldy,1899)Among the collected species ,six were regarded as new records to the Iraqi fauna of this order,they were : A. nebularis,M. vettigera( macrpterous) , M . vettigera( apterous), Ma.hornii , N.cinerea , C.bonsdorffii andP.leachi.Study also deal with the measuring of some ecological aspects like air and water temperatures , salinity , pH and dissolved oxygen ,and their effected on the monthly distribution of the aquatic Hemiptera. Temperature was the most effective aspect in the monthly distribution of the insects ,as the lowest numbers were recorded in Summer months and the highest number were in Winter months , the ranges of the air temperatures were 9 - 43º C and water temperatures were 2 - 33º C .The highest concentrations of salinity was recorded in Al - Zubair , Abu Al - Khasib and Al - Faw reached to 35g/l in Al - Zubair in July , the pH ranges were the same in all stations and was alkalid , their ranges were 7.3 - 7.8 , about the dissolved oxygen it was low and same in all stations except in Sullein Marsh which reached to 11 mg/l in January .The concentration of some heavy metals in water also studied like Fe, Pb, Cu and Zn , the highest concentrations were to Fe and Zn in Basrah center and Al - Zubair, and the highest concentration for Fe in Al - Zubair reached to 6362.21μ/L in August, while highest concentration for Zn in Basrah center reached to 1365.92μ/L in July, while in the other stations the concentrations were lowest .The study also used the water boatmen Sigara latelaris(Leach,1817) as bioindicators of heavy metals pollution in the water of Basrah provience , and taken Sullein marsh as control , and measuring the concentrations of the heavy metals in insects tissues , water and sediments , the results showed that the insects collected from the water of the center of the city can accumulate Fe andAbstractCZn in their tissues and the highest concentrations reached to 3562.16 and 3778.12 μ /gm for Fe and Zn in July , while in the insects taken from Sullein marsh were 286.25 and 285.17 μ /gm .The concentrations of heavy metals in water and sediments of the city center were measured and the highest concentration was for Fe and Zn , and reached to 1569.91 and 1365.91 μ /L of the water of city center , while reached to 215.01 and 223.14 from the same metals in the water of Sullein marsh , the concentrations of the same metals were recorded in sediments of the city center and reached to 2865.16 and 1982.56 μ /gm for Fe and Zn , and the highest concentrations of the same metals in Sullein marsh were 418.57 and 393.74 μ /gm .

دراسة جزيئية للجينات البيتا - لاكتاميز في بكتريا Acinetobacter baumannii المقاومة للمضادات الكاربابينيم == Molecular Study of ? - lactamase Genes in Carbapenem Resistant Acienetobacter baumannii (CRAB)

Author name: ربيع عبد الاله مجيد عبد علي الهنداوي
Supervisor name: ايمان محمد جار الله
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: English
University location: Babylon
First pages:
Abstract: During the period between April 2016 and January 2017, a total of 1100 clinical specimens (burns, wounds, throat, urine, blood) were collected from two hospitals in Babylon province Al - Hillah Teaching Hospital and Medical City of Mirjan Hospital. All specimens were cultured and 17 Acinetobacter baumannii isolates were obtained from clinical specimens in a percentage of (1.5%) distributed in burn 11 (2.8%), wound 1 (0.6%), throat 1 (2%), urine 3 (0.7%) and blood 1 (0.9%).This study was conducted to determine the presence of carbapenem resistance Acinetobacter baumannii (CRAB) isolates and the prevalence of β - lactamase genes responsible for carbapenem - resistance among clinical isolates of A. baumannii collected from two hospitals in Hillah City. Isolates were identified according to VITEK2 system. Antibiotics susceptibility was assayed by using disks diffusion method according to CLSI recommendations and it’s found 8 isolates was carbapenem resistant.The eight CRAB isolates were tested for biofilm formation by using the tube adherence test and the results showed that 5 of 8 tested isolates (62.5%) were strong for biofilm formation. Two genes related to biofilm formation was investigated (bap & blaPER - 1 genes). Six isolates were positive for the bap gene (75 %) and no isolates had positive for blaPER - 1 gene. The eight CRAB isolates were tested also for the presence of two virulence factor genes include espA (K1 capsular polysaccharide) and ompA (outer membrane protein A), this detection was performed by the conventional PCR technique. The positive result for espA and ompA genes was (62.5%) and (75%) respectively.IIPhenotypic detection of carbapenemase production was performed using the imipenem - EDTA disk and modified Hodge's test (MHT). Then isolates were subjected to monoplex PCR targeting blaCTX, blaTEM, BlaSHV, blaIMP, blaVIM, blaSPM, NDM - 1, NDM - 2, AmpC, OXA - 23, OXA - 24, OXA - 48, OXA - 51, OXA - 58 and OXA - 235 genes, all CRAB isolate gave positive result with MHT and (50%) of these isolates were positive to the imipenem - EDTA disk. PCR experiments showed ; all CRAB isolates were harbored blaOXA - 51 gene, six (75%) isolates were harbored blaCTX gene, four (100%) isolates were harbored blaTEM gene, four (50%) isolates were harbored blaVIM gene, four (50%) isolates were harbored blaSPM gene, seven (87.5%) isolates were harbored NDM - 2 gene, seven (87.5%) isolates were harbored ampC gene, six (75%) isolates were harbored OXA - 23 gene, one (12.5%) isolates were harbored OXA - 24 gene, two (25%) isolates were harbored OXA - 48 gene and one (12.5%) isolates were harbored OXA - 235 gene, while none of these isolates harbored BlaSHV, blaIMP, NDM - 1, NDM - 2 genes. All isolates of A. baumannii appear as MDR, while two isolates appear to be as XDR.These results revealed that CRAB isolates constitute 47% of all A. baumannii that had been isolated from Al - Hillah Teaching Hospital and Medical City of Mirjan Hospital in Babylon province and some of carbapenemase were detected in both phenotypic and genotypic methods (PCR). This underlies the importance of their accurate identifications and reporting to prevent the emergence of complete resistance to the most potent drugs against A. baumannii in Babylon province.

دراسة جزيئية ومناعية لطفيلي اللشمانيا الجلدية Cutaneous Leishmania في المحافظات الوسطى والجنوبية == Molecular And Immunological Study of Cutaneous Leishmania In The Middle And Southern Provinces

Author name: ازهار موسى جعفر الموسوي
Supervisor name: علي حسين الكبيسي | مهدي حسين العمار
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Karbala
First pages:
Abstract: The study includes distribution of the Cutaneous Leishmania in some governorates in Iraq. Specimens are included cases of outpatients in hospitals like : Al - Karamah, Al - Sadir Teaching Hospital in Baghdad, Al - Hilla Teaching Hospital, Al - Hussein Teaching Hospital and Ein Altamer General Hospitals in Kerbala, Al - Sadir Teaching Hospital in Al - Najaf, Al - Karamah and Al - Zahraa Teaching Hospitals in Wasit, Al - Diwanyia General Hospital. Al - Hussein General Hospital in Al - Nasiriya, Al - Smawa General Hospital, Al - Sadir Teaching Hospital and Al - Qurna General Hospital in Al - Basrah during October 1122 to march 1121 Cutaneous Leishmania is consider a major problem that faces the patients because it causes deformities in the infection region. The study includes 330 cases of skin ulcer in which 225 of them were microscopically positive. Parasitic growth was done using two types of cultural media NNN and RPMI - 1640.The results have shown a considerable difference between male and female in which male was significantly exceeding 131) %22.11 (and female was 94) %72.44 (and 139) %72.44 (of outpatients from rural areas increased in numbers from outpatients of urban areas, and only) %12.11(from urban areas.The number of ulcerations in body has exceeded infection, that means more than one ulceration in male 83 (85%) and for female 41 (62%), and concerning infection in three or four ulceration, it is approximately equal and normally distributed on face areas 99 and a percentage of (44%) then lower arms are 92 and a percentage of (40.80%) and the upper parts are 33 and a percentage of (14.66%). Also, one infection was recorded on The study shows that 46 patients in a percentage of (%20.44) having a dry infection; whereas 179 patients (%79.55) having the wet type.For accurate diagnosis of Leishmaniaa species distributed in Iraq, PCR Was used technique and diagnosed two types of Leishmania parasite that causes the illness. A band in a length of 560 bp in 186 samples related to Leishmania major and the band sample was 750 bp related to 39 Leishmania tropica.Through studying the samples in Kerbala City, the number infected samples were 125 for the years of 2010 - 2011 the actuality is (73) infection and (52) infection from 2011 - 2012 in a ratio of (58.4 % and % 72.7 accordingly) in different inhibited areas in the governorate in which (51)) %71.2 (was in Ein Al - Tamir Suburb, and 29 cases of) %11.1 (in Al - Husseinya Suburb, as well as 17 cases of (13.6%) in Al - Hur Suburb, 16 cases) %21.2 (in the south quarters and 7 cases of (5.6%) in the north quarters and only 5 cases of (4%) in the city center.The molecular diagnosis in Polymerase Chain Reaction for Kerbala governorate samples have shown 31) %17.2 (of L.tropica, 22) %24.7(of them were male and 9 of) %4.1 (of them were female. L. major was 94 of) %42.1 (in which 55 of) %77 (were in male and 39 of) %12.1 (were in female.The immunological study for the patients serums with L. major by ELISA technique has significantly shown raised values of IgG and IgM during infection in comparison with the control group, then it declines slowly after treatment in which IgG was in ratio (1811.1 ± 523.1 mg/dl), and IgM was in ratio (166.7 ±23.6 mg/dl). Also, in L. tropica - IgG was in ratio (1722.1 ± 524.0mg/dl) and IgM was in ratio (25.3±182.9mg/dl) in comparison with control specimens, and then the ratios have declined after treatment dosages with Pentostam drug.The cellular dynamics values have shown an abstract increase in which interferon - gamma (IFN - ?) in infected patients with cutaneous L. major were (221.1±2.2mg/dl) which declined after treatment abstractly to (7.12±1.1mg/dl) as well as for L tropica (22.1±7.2mg/dl) which shows no significant abstract difference after treatment with control.An increase appears in cytokine IL - 10 that reached in L.major (122.1±8.2mg/dl) and after treatment was (8.11±2.2mg/dl) and in L. tropica was (222.1±2.2mg/dl) with no significant abstract difference after treatment with control specimen.As a simple attempt to find a vaccine for Leishmania disease a Lipophosphoglycan was isolated and purified as known factor for promastigot stage. The vaccine injected for two groups of Bulb mice in a concentration of purified vaccine (L.majorand Ltropica) to study the immunological response by detecting the Lymphocyte Transformation assay, the Delayed Type Hypersensitivity test and the PhagocytosisIndex.In lymphocyte transformation the ratio of L. tropica was % 7.2 and for L. major was % 21.7 and there is significant difference by P? 0.05 in comparison with control specimen in which its ratio was %7.4, and for Delayed Hypersensitivity test study the average foot thickness injected with vaccine and the other foot which injected with phenol - saline solution - only (1.11 ± 2.12ml) for the first group and (1.21 ± 2.72) for the second group, and for the control group it has registered (1.77± 1.15 ml) with a considerable difference of P? 0.05. By calculating the ratio of Phagocytosis Index cells, it was 17% and 27.6% for the first and second groups while for the control group it has been reaching 9.10%. Therefore, we can get advantage from these values as indicators for the rule of vaccine in the immunological response and triggering of vaccine idea for this disease.

التاثيرات الوراثيه الخلوية وموت الخلايا المبرمج للسيرمايد في الخلايا السرطانية للخطوط الخلوية و الحيوانات المختبرية == Cytogenetic And Apoptotic Effects Of Creamide On Cancer Cells (In Vivo And In Vitro)

Author name: مثنى ابراهيم ملك البدري
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
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التاثيرات الكيميائية المانعة لمثبط السايكلواوكسجينس-2 ، ميلاتونين و تداخلهما على التسرطن الكبدي المستحدث بثنائى اثيل نايتروزامين في ذكور الجرذان المختبرية == Chemopreventive Effects Of Cyclooxygenase-2 Inhibitor, Melatonin And Their Combination On Diethylnitrosamine-Induced Hepatocarcinogenesis In Male Albino Rats

Author name: طوران قادر عثمان
Supervisor name: صباح ناصر العلوجى | الماس محمد رشيد
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
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دراسة بيئية تصنيفية للهائمات النباتية في رافدي العظيم وديالى وتاثيرهما في نهر دجلة == Ecological and Taxanomical Study for Phytoplankton in Al-Adaim and Diyala Tributaries and their Effects on Tigris River

Author name: منار عبد العزيز عبد الله الصراف
Supervisor name: حسين علي ناصر السعدي | ثائر ابراهيم قاسم
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Baghdad
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دور فيتامين "C" كمادة مانعة للتاكسد على بعض معايير الدم في الانسان == The Role of Vitamin "C" as Antioxidant on Some Human Blood Parameters

Author name: سامي رحيم الكاتب
Supervisor name: فاخر سلمان العاني | هناء حنين منكلو
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Baghdad
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بعض الفحوصات المصلية والبايولوجية في تشخيص مرض اللشمانيات لدى الاطفال في منطقة الفرات الاوسط : دراسة مقارنة

Author name: هاشم رحيم طارش
General topic: Biology
Specific topic: Life Science
Degree: Doctorate

Systematic Study Of The Genera Of Labiate In Iraq

Author name: عادل موحان عداي
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University of Baghdad
University location: Baghdad

A Study Of Certain Immunological Parameters In Women Afficted With Breast Cancer In Iraq

Author name: نبيل ايليا وحيدة
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University of Baghdad
University location: Baghdad

دراسة بيئية وتصنيفية للجنس potamogeton L.في نهر دجلة المار بمحافظة نينوى

Author name: مي طه حامد الوتار
Supervisor name: بشير علي بشير النعمة
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University location: Mosul

دراسة الاكريتارك في تكوين الخابور (الاوردوفيشي الاعلى) من بئر خليصية - 1، ال غرب العراق

Author name: نزار عبد الواحد مصطفى
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University location: Mosul

تاثيرات المستخلصات المائية لثمرة السبحبح melia azedaragh والحنظل citrullus cologynthisعلى حيوية الطفيلي الليشمانيا الدونوفانية leishmania donovani في كبد ذكور الفئران المهقاء mus musgulus

Author name: كوثر ابراهيم فتاح الهرمني
Supervisor name: زهير ابراهيم فتوحي رحيمو | حسين اسماعيل ارتين الخان
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University location: Mosul

دراسة فايروسية ومناعية على مرضى التهاب الكبد الفايروسي نمط C

Author name: محمد دواج
Supervisor name: باسمة احمد عبد الله
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University location: Mosul

تشخيص فايروس موزائيك الفاصوليا الاصفر على الباقلاء باختبارات بصمة النسيج النباتي ولاليزا والبقعة المتفشية وتحضير مصلة المضاد

Author name: نهال يونس محمد
Supervisor name: نديم احمد رمضان الحمداني
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University location: Mosul
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