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دراسة بيئية لبعض العناصر الثقيلة في مياه نهر الفرات عند مدينة الناصرية وقابلية مراكمتها من قبل بعض الطحالب الخضر المزرقة ونبات الشمبلان مختبريا == Ecological Study of Some Heavy Metals on The Euphrates river Water at Nassiriyah City and The Ability of Accumulation by Some Blue green Algae and Ceratophyllum demersum L. in the Laboratory

Author name: عبد الوهاب ريسان عيال العبادي
Supervisor name: احمد محسن عذبي | اقبال جاسم الاسدي
General topic: Biology
Specific topic: plant - aquatic life
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The diversity of phytoplankton were studied and some physical and chemical environmental factors are (Temperature of air and water , pH , electrical conductivity , salinity , dissolved oxygen , alkalinity , hardness and nutrients) of four locations on Euphrates river water at Nassiriyah city in Thi Qar governorate . The first location lies north - west of Nassiriyah city at Al - Sharif region , whereas the second location lies in Al - Mustafaweia region , and the third location lies in Al - Majzarah region , and finally the fourth location lies in Al - Fadlyia city . Samples of water were collected from January to December of the same year of 2014 .The concentrations of some heavy metals are (lead , cobalt and cadmium ) were estimated , in addition to limitation of the efficiency of some algal species and Ceratophyllum demersum L. in bioremediation of their elements on the river water and a limitation of the accumulation sites of the heavy metals on the cells of the algal and plant by using the scanning electron microscope - x - ray . The results revealed clearly a variation on monthly , quarterly and locations of the number properties of the physical and chemical that were studied , the temperature of water ranged between 11.5cہ - 43.6c ہ and air ranged between 15.5cہ - 44.9 cہ , the high temperature at the second location was recorded .The high value of pH 8.62 in the forth locations at April was recorded and the low 7.2 in the third location at July . The concentrations of electrical conductivity were ranged between 3308 - 5773 μs / cm , whereas the concentrations of salinity ranged between 2.11 - 3.69 gm / l , the high concentration on the third location at February was recorded , and the low on the first location at April was recorded , while the concentrations of the dissolved oxygen ranged between 5.40 - 11.70 mg / l , the high concentration on the first location at February was recorded , whereas the low concentration on the third location at August was recorded , also the alkalinity concentrations ranged between 13 - 250 mg / l , the high concentration on the fourth location at March and the low concentration on the third location at July was recorded . From the results , it was show that , the river water was hardship , the hardness concentrations ranged between 850 - 1610 mg / l , the high concentrationaon the third location at January , and the low on the second location at May was recorded .While the concentrations of the nutrients , as nitrate and phosphate , the nitrate concentrations ranged between 13.6 - 41.5 mg / l , the high concentration on the third location at February and the low concentration on the first location at July , whereas the phosphates concentration ranged between 0.83 - 1092 mg / l , the high concentration on the third location on first location at April.Also the diversity of phytoplankton had been studied that were present in water of the river, There were diagnosis 198 species distributed between six classes , the dominance to the Bacillariophyceae as 90 species which belonged to 19 genus were recorded and at the percentage 45.45% of the total numbers of species recorded , followed by the Chlorophyceae 46 species which belonged to 25 genus and at the percentage 23.23% of the total species were that were recorded , followed by Cyanophyceae algae 46 species which were belonged to 15 genus and at the percentage 23.23% and algae of the Euglenophyceae had eight species which were belonged to three genus record and at the percentage 4.04% , with regard to the algae of Xanthophyceae had recorded five types at there belonged to three genus and at the percentage 2.52% and finally the algae of Chrysophyceae which was recorded three types belonged to the same genus , which collectively represent the percentage 1.51% .The study also experienced a record for species of phytoplankton at the first time in Iraq , which were five belonged to four classes , one of them was belonged to Chlorophyceae algae (Trochiscia granulate Prescott.) and the others to the Euglenophyceae(Phacus pyrum (Ehrenb.) Stein and Trachelomonas simillis Stokes) and one species for Chrysophyceae(Mallomonas elliptica (Kisselew) Conrad)and Xanthophyceae algae (Peroniella planctonica G.M.Smith).The concentration of some heavy metals (lead , cobalt and cadmium) in water of the locations was studied by using the flame atomic absorption spectrophotometer , the results were showing that , the concentrations of lead ranged between 4.58 - 29.0 μg / l , as record the high concentration on third location at August and the low on the first location at December , while the concentrations of cobalt were ranged between 4.60 - 23.0 μg / l , as record low concentration was on the first location at December ,bwhile the high concentration on the third location at August , cadmium concentrations ranged between 0.84 - 14.63 μg / l , the low concentration 0.84 μg / l recorded in the first location at December , while the high concentration 14.63 μg/ l in the third location at June .The study included too . a laboratory experiments to demonstrates the efficiency of some species of algae and Ceratophyllum demersum L. on the bioremediation of pollutants in the river water . The results indicates that , there was an increase on the percentage of the removal of elements with the increasing concentrations of elements that were added to water aquarium , reaching the low percentage of biological remove of the lead 95.2% at the treatment 50 mg / l and at the time period 10 days , whereas the biological remove of the high percentage 99.2% at the same treatment during the time period 40 days.As for the cobalt element , the results showing that , there were a gradual increases of the proportion of the removal with the increasing concentrations of cobalt that were added to the water aquarium , and reached to 94.2% at the treatment 50 mg / l at the time period 10 days , whereas the percent for the removal 98.56% at the time period 40 days at the same treatment , whereas the percentage of the vital removing of the element cadmium by the water plant Ceratophyllum demersum was increases in removing from the water aquarium that was 92.6% at the treatment 50 mg / l at the time period 10days , whereas it was 97.40% at the same treatment .As for the algae it had been isolated and diagnoses , the were four species of , blue - greens (Microcystis aeruginosa Kütz. , Nostoc linckia(Roth) Bornet and Thuret , Oscillatoria amoena(ktz.) Gomont and Hapalosiphon aureus West and West , samples were collected from the Euphrates river water on the studied locations . It was purified and propagation of these species on the laboratory for a single culture cell , unialgal culture and axenic culture to test their ability to remove and accumulation of the heavy metals from water. These species of algae were treated salt solution of heavy metals are lead , cobalt and cadmium both in privates and at different concentrations 0.10 , 0.25 , 0.50 , 1.00 and 2.00 mg / l for a period of two weeks . H.aureus was accumulated more of lead 1.724 μg / gm at the treatment 2.00 mg / l , followed by O.amoena which accumulated 1.638 μg / gm and N.linckia accumulatedc1.621 μg / gm and finally M.aeruginosa accumulated 1.579 μg / gm at the same treatment , but when adding the salt solution of cobalt nitrate , the alga O.amoena was accumulated more of cobalt element 1.726 μg / gm at the treatment is 2.00 mg / l , followed by H.aureus as accumulated 1.638 μg / gm , and was N.linckia accumulated 1.535 μg / gm , followed by M.aeruginosa 1.428 μg / gm at the same treatment . When are using the solution of the salt cadmium acetate , the N.linckia was accumulated more of cadmium 1.08 μg / gm , followed by H.aureus which was accumulated 0.63 μg / gm and finally O.amoena accumulated the percentage was reached 0.59 μg / gm at the same treatment .The study was included too the a limitation sites and accumulation of heavy metals within the plant cells and algae that were studied by using technology of scanning electron microscope - x - ray and the use of reagent X - ray EDX with SE2 .The results were showing that , The locations which accumulated heavy elements was in the cytoplasm of the cell . On the above of the foregoing the Ceratophyllum demersum L. showing high efficiency of accumulation of heavy elements inside the cells as compared with the studied algae , the efficiency and ability to increase of the accumulation increases by added of elements and by the increases period of exposure , also algae were revealed a capabilit

دراسة الطفرات الوراثية في جيني TPO وTSHR في محافظة البصرة

Author name: فله عبد الستار
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Plant - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: Considered the thyroid glands task Deaf which produces important hormones are Tri iodothyrosine T3 and Thyroxin T4 and is responsible for their production a number of genes that encode the enzymes and proteins and the occurrence of any defect in this gene lead to the emergence of disorders of the thyroid work, including the case of palaces and fragments in hyperthyroid through the emergence of different pathological symptoms that indicate the occurrence of one of the cases studied in this research gland disorders at the level of molecular genetics .The study included only females were collected blood samples from 70 patients divided into 35 blood samples of the state of palaces and 35 blood samples for the case of the bulk in hyperthyroid deaf and diabetes endocrine centers of hospitals and ports Fayhaa year and Qurna, as well as from laboratory d. Nizar Mahfouz and represented a group of patients and 20 blood samples from healthy people represented as a set of control and that during the period from April to September 2013. But has been working on a sample of 70 patients (35 blood samples by the state of palaces and 35 blood samples for the case of the bulk gland) to add to the 20 blood samples of control and ages of the two groups and the healthy range (12 - 60 years).DNA was extracted DNA of two and three encoded amplified regions (8, 9 and 14) for the TPO gene and encoded region tenth TSHR gene PCR technology after it was diagnosed and analysis of mutations using the method for Sequences .The results showed the presence of two types of changes in the TPO and TSHR genes , two polymorphism (SNP) and Mutation, in gene156TPO study found hereditary forms c.1117G> T, A372S and c.1194G> C, S398T in the two groups of patients and healthy and not they relate to the occurrence patients palaces gland activity, as the study diagnosed 12 Novel Mutation in exon 8 in 21 patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all mutations diagnosed are mutations bitmap compensatory (substitution) the type of guarantee or Transtion Transversion depending on the nature of the base mutant, five of the mutations are Missense Mutation in 11 infected condition limitations are as follows c.949A>G,M317V , c.1064G>A,R355H, c.1277C>G,A425G , c.1063C>A,R355S and c.1207C>A,L402M and seven silent mutation in 10 patients hypothyroidism c.1062G>A,A354A , c.1050C>A,L350L , c.843C>A,A281A , c.1101G>A,V367V, c.1143C>T,I381I , c.1071G>A,R357R and c.2406C>T,D802D , While the results did not show any difference in the analysis of the genetic sequence of the two exons 9 and 14 at comparable with the genetic sequence of the control group. In this study suggests that there may be a correlation between the occurrence of mutations in a gene TPO and the state hypothyroidism . The results of the current study showed that the gene TSHR has an impact in both cases, and shortcomings in fragments in the activity of the gland where the study found three types of polymorphism (SNP) of the two of them Non - Synonymous type c.2181G> C, E727D and c.1489G> C, A496P and shape The last of the silent type genetic c.1377G> A, A459A, as the results showed that the two forms hereditary c.2181G> C and c.1489G> A have no correlation to happen disorders and are globally registered the shape genetic c.1489G> C, A496P did not score previously has current study for the first time and may have a link to happen one of the disorders depending on the genetic predisposition of the individual . I found the157current study, seven Novel Mutation in exon 10 distributed to five mutations among patients hypothyroidism as compared to control (who did not appear to have any kind of mutations diagnosed) and that all the mutations diagnosed are mutations bitmap compensatory (substitutive Substitution) to ensure the kind of Transtion or Transversion depending on the nature of the base mutant 'three mutations are Missense Mutation in 3 people with the status of the palaces and the proportion of each mutation them (20%) are as follows c.1330 T>C , Y444H ; c.1424 T>C ,L475P and c.1435 T>C , S479P and two silent mutation in patients hypothyriodism c.1338 G>A, L446L and c.2300 G>A , Untranscrib , While the results recorded and there is only Tafrtin in the case of the bulk in hyperthyroid by (5.71%), one Missense mutation (c.1832 C> A, P610Q) and the other is silent (Silent) (c.2103 C> A, R701R). The present study indicated that the probability of the occurrence of the disease mutations link through the site mutations in specific areas to link hormone catalyst and the interaction between the Receptor and the unity of secondary alpha (G - protein). Results of the current study also showed that mutations identical and disparate factors have an impact on events in the case of deficiencies, genetic gland activity in the TPO and TSHR through defect events and constructed partially or wholly in the function of the protein encoded by genes TPO and TSHR

عزل وتشخيص مركب ايضي ثانوي جديد لكل من الفطرين Fusarium Trichoderma harzianum وsolani المعزولين من السواحل الرسوبية في محافظة البصرة وتقييم فعاليتهما الحياتية == Isolation and Identification of Novel Metabolic Compound From Both Fusarium solani and Trichoderma harzianum Isolated From Sedimentary Coast of Al - Basrah City and Estimate their Activity

Author name: فردوس نوري جعفر
Supervisor name: محمد لطيف علي | نيران جاسم الصالحي
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: with the interest of medical and pharmaceutical uses of the natural products from marine fungi , thirty species of fungi belong to ninteen genus were isolated and identified from sea coast sediment of Al - Faw and Al - Seeba .The fungi Acremonium strictium , Geotricum candidum, Fusarium solani ,F.oxysporum , Penicilium sp. ,Trichoderma harzianum exhibited high inhibition activity toward growth of two reference strains of bacteria Escherichia coli (ATCC25922) and Staphylococcus aureus (NCtC 6571). The six selected fungi were grown in liquid culture media PDB at 25°C for two weeks ethyl acetate was used to extract the active compounds from thefermentation liquid media while ethanol was used to extract the active compounds from fungal mycelia .The crud extracts (fermentation and mycelium ) showed different inhibition activity against the pathogenic bacteria , Proteus mirabilis , S. aureus, isolates E. coli, Pseudomonas aeruginosa Klebsella pneumoniae. and pathoginic yeastes Candida albicans, C.tropicals,Cryptococcus neoformans and dermatophytic fungi Trichophyton verrucosum and Microsporium canis . The results revealed that the fermental fungi extracts has more inhibition activity than mycelial fungal extracts.The minimal inhibitory concentration (MIC) of fungal crude extracts were determined against gram positive and gram negative bacteria and pathogenic yeasts and fungi . antagonisum between studied fungi were evaluated , and the fungus T.harzianum has been the more antagonistic effect against other fungi. The toxicity of the fungal crude extracts were tested by using of human RBC at different concentration the result of this experiment showed that all extracts are non toxic except F.oxysporum extract.From all the above results the both F.solni and T.harzianum extracts showed higher activities ,thus these two extracts chosen for further studies. The anticancer activity of both extracts were studied against Hela cell line and the anticancer activity appeared at 250 Mg/ml and 62.5Mg/ml respectivily. The antioxidant activity of extracts were examined by three different methods ,B - carotene ,hydrogen peroxide scavenger and ferrus ion reduction , the results of three methods showed that both fungal extracts have antioxidant behaviours and the T.harzianum extract had more antioxident activity than F.solani extract and the antioxidant activity of both fungal extracts were increased as the concentration of the extracts increase.An optimization of the fungal growth and bioactivity of both fungi were conducted, by using different Nitrogen sources ( Asparagine , Yeast extract , peptone , Ammonium chloride , Sodium nitrate , Ammonium sulphate ) and carbon sources ( Maltose , Galactose , Glucose , Dextrose , Sucrose ,xylose and Starch ) the results elucidated that yeast extract was the best for growth of both fungi on solid and liquid media and high inhibition activity appear with T.harzianum against bacteria isolates , the carbon source starchenriched the growth of F.solani with more activity in inhibition the bacterial growth while the sucrose give high enrichment to the growth of T.harzianum in solid and liquid media with high activity toward bacterial growth. The study also indicates that the beast temperature and pH which gives the higher activity and growth 25°C and pH6 . The effect of different concentration of salinity were studied as well (1%,2%,3%,4%,5%,6% NaCl)and 2% showed the best concentration .The activity of two studied fungal extracts were superior than commercial antibiotic toward bacterial isolates .Preliminary qualitative chemical tests were carried out on the extracts and their chemical nature were identified .TLC technique were used and the chemical analysis showed that F.solani extract consist of two components with constant Rf values, the components marked as A1 and A2 . A1 shows higher activity against bacterial strains while T.harzianum extract consist of one component.The chemical structures of the isolated compound were

دراسة تصنيفية وبيئية لبعض انواع عائلة ذباب الخيل Diptera : Tabanidae ودورها في نقل طفيلي Trypanosoma evansi في محافظة البصرة

Author name: علاء ناظم حاتم علي
Supervisor name: ضياء خليف كريم
General topic: Biology
Specific topic: Zoology - Insects
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

التغيرات الكيميوحيوية المصاحبة للشد الملحي وتقييم دور مستخلص الطحلب الاخضر Cladophora crispata في نبات الرز Oryza sativa L. المستزرع نسيجيا

Author name: عبد الامير رحيم عبيد
Supervisor name: حسيي خلف زاير | لوى حسين عبد القادر
General topic: Biology
Specific topic: Plant - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study was conducted at the tissue culture laboratory at the Dept. of biology, College of Education pure Sciences from 1/3/2012 - 1/12/2014.With the objective of studing the tolerance of the salt stress of two rice (Oryza sativa L.)cultivars namely Anber and Furat using the technology of tissue culture. . Mature seeds (mature embryo and endosperm) were used as bing parts of explants which were cultivated in a semi - sold( Murashige and Skoog 1962) nutrient medium and some additives such as vitamins and amino acids .The growth regulator Dichlorophenoxy acetic acid (2.4 - D)was used, about 8mg/l , sucrose at 30g.l - 1 . . The study ,also ,tested the effect maltose at 30g.l - 1, and the effect of 0.5g/l of casein hydrolest were tested.the study also included the different of sea water concentrations (0,20,40,60)%on the induction of growth and development of callus ,the effect of green algae extract at Cladophora crispate by adding 1g fresh weight to the nutrient medium with all sea water concentrations.All the features of the callus were tested as well as the effect of the interaction between sea water concentrations and algae extract , as well as interactions among rice cultivar x sea water concentration x algal extract.The study depended on some growth indicators to determine the degree of callus to tolerance towards salt stress .the resistance of callus to salt depended on regeneration through the required of time for the formation of embryos ,number of germinated embryos ,and number of plantlets .The concentration of the major metal ion : sodium, potassium, phosphorus, calcium ,magnesium, potassium/sodium percentage , and the minor metal ions : iron ,copper, manganese were also some chemical components such as protines and amino acid proline measured, were also measured .The randomized block design (C.R.D)and factorial experiments were used depending ten replicates per treatment .The results can be summarized as follows : 1 - The results showed that the addition of sugar di maltose as an energy source and Casein hydrolyses to the nutrient medium had appositive effect on significant decrease in the required time to mitiate the callus ,in the increase of callus induction percentage and in the increase of freshweight of initial callus. .2 - The effect of sea water and algae extract : . A - The current study shaued that the addidtion of 20 and 40 % sea water to the nutrient medium resulted in a significant increase in the required time to induce the callus ,and a significant decrease in the percentage of callus induction and weight of the mitial callus as compared with the control treatment .The concentration 60%0f sea water caused the mature seeds to lose their ability to germinate and induce the callus if no induction occurs at all . The effect of algae extract resulted in a significant decrease in the required time for callus induction ,and a significant increase in the percentage of callus induction ,the weight of the mitial callus and 0,20,40% of sea water as compared with free algae extract medium.B - The results showed that the addition of 20% of sea water to the nutrient medium that is prepared for the growth of the embryo callus led to a significant increase in the fresh weight as compared with the control treatment , whereas the two concentrations 40 and 60%sea water led to a significant decrease in the embryo callus weight .The effect of the algae extracted to a significant increase in the fresh weight of the embryo callus with sea water concentrations as compared with the free algae extract medium.C - The addition of 20,40 and 60%sea water cause a significant decrease in the number of the somatic embryos ,thir germination auerage ,number and germination of the formed plantlets . This addition also caused a significant increase in the required time to stare the germination of the somatic embryos as compared with the control treatment .The addition of the algae extract led to increase the number of formed embryos ,germination percentage ,and number and percentage of plantlets ,wher was the addition led to lower the required time to germination the embryos compared with the free algae extract medium.D - The addition of 20 ,40 and 60% sea water to the nutrient medium resulted in a significant increase in the concentration of sodium ,calcium ,and magnesium ion in the embryo callus ,while a significant decrease occurred in the concentration of potassium and phosphorus ion and thedpotassium/sodium percentage as compared with the control treatment .The abone mentioned concentrations 0f sea water led to significant increase in concentration of ion ,copper, and zinc ions ,and significant decrease in manganese ion ,The addition of the algae extract caused in lowering the concentration of sodium ion .This decrease was significant in the 60 % sea water medium. The ingrease of potassium ,calcium ,phosphorus, and magnesium ion in embryo callus tissue when compared with free algae extract medium and was significant with some sea water concentration.E - The results ,also ,showed that the addition on 20, 40 and 60% concentrations0f sea water resoled in a significant decrease in concentration of some chemical ingredients such as protein and amino acid proline of the embryo callus in comparison with the control treatment . as far as the effect of the algae extract is concerned ,the addition of this extract to the nutrient medium led to a significant increase in the concentration of the proteins and amino acid prolne as well as with all sea water concentrations.F - The results of study showed that the addition of 20,40,60% sea water led to a significant increase in the rate 0f effectiveness 0f the enzyme Super oxide dismutase (S.O.D) and enzyme Ascorbate peroxides (APX),reaching the highest rate of effectiveness of the enzyme at the concentration 0f 60% compared with the comparative treatment , whil the highest average of the effectiveness of enzyme Catalase (CAT)and glutathione piroxidase (GP)and polyphenoloxidase (ppo)was at the concentration 20%sea water then it started to decline when increasing the concentration of sea water from 40 to 60% As for the algae extract it has been shown from the results of the current study that the addition of algae extract to nutrient medium to a high effectiveness of enzyme SOD,CAT ,APX and GP and more significant with all concentration of sea water The impact of algae extract on the effectiveness of the enzyme PPO has led to increasing the effectiveness of this enzyme but did not amount to a significant level except with sea water nutrient medium .G - The results of the current study showed a significant increase of the Amber cutivar in the percentage of the callus induction and the required time of this induction as well as the Wight of the initial and embryo callus ,number and germination percentage of thesomatic embryo , number of plantlets ,as wall as the percentage of the accumulation of potassium ,phosphorus ,calcium, and manganese ions ,as will as in the potassium/sodim ratio focus histidine proline and the effectiveness of the enzyme SOD and CAT enzyme and enzyme GP. While the product was higher than in the accumulation of Firat magnesium ion ,zinc copper and in the effectiveness of the enzyme in APX & PPO . .

واقع التلوث بالمركبات الهيدروكابونية في المياة والرواسب وبعض الاحياء المائية في مهر الكحلاء محافظة ميسان / العراق == The Status of Hydrocarbon Compounds Pollution of Water, Sediments and Some Aquatic Biota in Al - Kahlaa River - Missan Province /Iraq

Author name: صالح حسن جازع
Supervisor name: عبد الحسن يوسف العضب | حامج طالب السعد
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: English
University location: Basrah
First pages:
Abstract: The present study was performed to have knowledge of hydrocarbon compounds pollution status in the water (dissolved and particulate fractions),sediments ,two species of aquatic plants (Ceratophyllum demersum and Paspalum pespaioides) ,two species of fishes(Liza abu and Carassius auratus) and one species of molluscs (Pseudodantopsis euphraticus) .The samples were collected monthly for the period November 2012 to October 2013 from four different stations ) Al - Magideh, Treatment unit, Al - Husaichi and Al - Zubair) distributed along Al - Kahlaa River in Missan province , in addition to Reference station lies on the Tigris River before entering Amara district about 25 Km .The sampling's months were divided into four typical seasons although this is incompatible with Iraq climate.As well as measures of some ecological parameters (water and air temperatures ,pH ,turbidity, electrical conductivity ,dissolved oxygen , biological oxygen demand BOD5 and total suspended solid) which ranged between 22 and 42 C˚ , 18 and 31 C˚ , 6.9 and 7.82 , 42 and 172 NTU , 12 and 118 μS/cm , 4.66 and 9.25 mg/l , 1.46 and 5.04mg/l ,33 and 108 mg/l respectively .In addition to that the Organic Matter(OM) and the texture were measured in the sediment samples. OM values ranged between 0.078 and 0.927% , whereas the percentage of the texture was ( 15 %clay, 71% silt and 14% sand),(31 %clay, 63% silt and 6% sand), (33 %clay, 57% silt and 10% sand) , (40 %clay, 47% silt and 13% sand) and (18 %clay, 55% silt and 27% sand) in Al - Magideh, Treatment unit , Al - Husaichi , Al - Zubair and Reference stations respectively .The concentrations of total petroleum hydrocarbons (TPHs)were measured by using spectrofluorometer. Concentrations of total petroleum hydrocarbons in dissolved fraction ranged between 1.36 μg/l in Reference station and 13.87 μg/l in Treatment unit station during summer and winter respectively ,and in particulate fraction between 2.69 μg/g dry weight in Reference station and 29.92 μg/g dry weight in Treatment unit station during summer and winter respectively ,while in sediments they ranged between 3.16μg/g dry weight in Reference station and 135.18 μg/g dry weight in Treatment unit station during summer and winter respectively. Concentrations of total petroleum hydrocarbons in C. demersum ranged between 5 μg/g dry weight during winter and 58.97 μg/g dry weight during summer , while in P. pespaioides between 3.18 μg/g dry weight during winter and 43.44 μg/g dry weight during summer. Concentrations of total petroleum hydrocarbons in L. abu ranged between 4.81 μg/g dry weight during spring and 37.3 μg/g dry weight during summer ,while in C. auratus between 2.51 μg/g dry weight during spring and 29.303 μg/g dry weight during autumn . Concentrations ofbtotal petroleum hydrocarbons in P. euphraticus ranged between 5.98 μg/g dry weight during spring and 119.75 μg/g dry weight during summer .The concentrations of Polycyclic Aromatic Hydrocarbons(PAHs) and n - alkanes were determined in the laboratories of Nihran Omer (South Oil Company in Basrah province ) ,using Gas Chromatography.Total concentrations of n - alkanes in dissolved fraction ranged between 0.027μg/l in Reference station and 0.192 μg/l in Al - Husiachi station during winter and between 0.016 μg/l in Reference station and 0.039 μg/l in Treatment unit during summer ,and for particulate fraction between 0.108 μg/g dry weight in Reference station and 2.096 μg/g dry weight in Treatment unit station during winter and between 0.101 μg/g dry weight in Reference station and 0.160 μg/g dry weight in Treatment unit station during summer ,while in sediments they ranged between 4.491 μg/g dry weight in Reference station and 51.32 μg/g dry weight Treatment unit station during winter and between 2.31 μg/g dry weight in Reference station and 19.31 μg/g dry weight in Treatment unit station during summer.The range of carbon chain length of n - alkanes in dissolved fraction ranged from C8 to C34 and in particulate fraction from C15 to C34 distributed in two patterns ,the first pattern included compounds between C8 and C25 with the abundance of C19,C21,C23 and C25 odd carbon number of and C18,C20,C22 and C24 for even carbon number ,while the second pattern included compounds with the range from C26 to C35 predominated by C27 and C29 odd carbon number and C26,C28 and C30 even carbon number.In sediments they ranged from C14 to C35 and distributed in two patterns, the first pattern included compounds between C8 and C25 with the abundance of C17,C19 and C21 odd carbon number and C18,C20 and C22 for even carbon number ,while the second pattern included compounds with the range from C24 to C35 with predominance of C31and C33 odd carbon number and from C24 to C34 even carbon number.Total concentrations of n - alkanes in C. demersum ranged between 6.467 μg/g dry weight during spring and 59.483 μg/g dry weight during summer ,whereas in P. pespaioides between 34.7 μg/g dry weight during winter and 166.6 μg/g dry weight during summer .The distribution of n - alkanes compounds in plants chain length ranged from C15 to C35 with bimodal from C15 to C27and from C28 to C35 with predominance of C17 to C25 odd carbon number and C18 to C24 even carbon number in the first one ,whereas in the second the dominance compounds were from C29 toC33 for odd carbon number and from C26 to C32 even carbon number.cTotal concentrations of n - alkanes in muscles of L. abu ranged between 14.60 μg/g dry weight during winter and 73.15 μg/g dry weight during summer and C. auratus between 34.76 μg/g dry weight during winter and 166.61 μg/g dry weight during summer . The distribution of n - alkanes compounds in fish muscles chain length ranged from C16 to C35 with bimodal from C16 to C25 and C26 to C35 with predominance of C17 to C25 and C18 to C24 for the first one ,and from C27 to C33 and from C26 to C34 for the second. These reflected nutrition nature for these kinds of fishes which depend on algae ,phytoplankton ,zooplankton ,diatoms ,insects and crustacean . While in the mollusc (P. euphraticus) ranged between 95.16μg/g dry weight during spring and 220.36 μg/g dry weight during summer. The distribution of n - alkanes compounds in P. euphraticus chain length ranged from C14 to C35 with bimodal from C14 to C27 and from C28 to C35 with predominance of C17 to C21 and from C18 to C24 for the first one ,and from C29 to C33 and from C30 to C34 for the second.The values of Carbon Preference Index (CPI) and pristane to phytane ratio was calculated to know the hydrocarbon compounds source in water ,sediments and biota of Al - Kahlaa River ,since the CPI value ranged between 0.142 and 1.314 in dissolved fraction and between 0.045 and 2.193 in particulate fraction , 0.160 and 1.872 in sediments,1.075 and 8.638 in plants , 0.285 and 1.537 in fishes and between 0.354 and 4.457 in mollusc .Whereas the values of pristane to phytane ranged between not detected and 1.579 in dissolved fraction , not detected and 0.601 in particulate fraction , 0.447 and 3.008 in sediments, not detected and 0.821 in plants , not detected and 2.176 in fishes and between 0.822 and 1.347 in mollusc .These indicate that there are two sources of hydrocarbon compound in water ,sediments and biota ,biogenic from living organisms and anthropogenic from domestic ,industrial and petroleum pollutants except in plants .Total concentrations of PAHs in dissolved fraction varied from 0.739 ng/l in Reference station to1.974 ng/l in Treatment unit during winter ,and from 0.300 ng/l in Reference station to1.125 ng/l in Treatment unit during summer ,and in the particulate fraction they varied from 0.79 ng/g dry weight in Reference station to 24.42 ng/g dry weight in Treatment unit during winter ,and from 4.369 ng/g dry weight in Reference station to10.545 ng/g dry weight in Al - Husaich during summer ,while in sediments they varied from 4.906 ng/g dry weight in Reference station to 35.479 ng/g dry weight in Treatment unit during winter ,and from 2.391 ng/g dry weight in Reference station to 25.886 ng/g dry weight in Treatment unit during summer.It had been noticed that there were a predominance of high molecular weight PAHs on low molecular weight PAHs ,while Benzo(a)dAnthracene/(Benzo(a)Anthracene+ Chrysene) BaA/(BaA+Chr) ratio ranged from 0.218 to 0.804 in water and 0.520 to 0.66 in sediments. The Indeno (1,2,3 - cd)pyrene /(Indeno (1,2,3 - cd)pyrene + Benzo(ghi)perylene) InP/(InP+BghiP) ratio ranged between not detected and 0.578 in water and between not detected and 0.681 in sediments .The Fluoranthene/ Pyrene (Fl/Py)ratio ranged between 0.150 and 2.47 in water and between 0.10 and 8.490 in sediments ,this give an indication of the source of PAHs compounds in water and sediments which were mainly pyrogenic and few from them petrogenic .Total concentrations of PAHs in C. demersum ranged between 24.124 ng/g dry weight during winter and 87.289 ng/g dry weight during summer ,whereas in P. pespaioides between 1.616 ng/g dry weight durig winter and 16.661 ng/g dry weight during summer .The Lower Polycyclic Aromatic Hydrocarbons to Higher Polycyclic Aromatic Hydrocarbons ratio ranged between 0.006 and 0.009 in C. demersum and between 0.008 and 0.103 in P. pespaioides. TheBaA/(BaA+Chr)ratio ranged from 0.995 to 0.997 in C. demersum and 0.756 to 0.995 in P. pespaioides. The InP/(InP+BghiP) ratio ranged between 0.342 and 0.807 in C. demersum and between 0.19 and 0.645 in P. paspaloides . The Fl/Py ratio ranged between 0.096 and 0.988 in C. demersum and between 0.018 and 5.018 in P. pespaioides. These indicate that the sources of PAHs in these plants were both pyrogenic and petrogenic .Total concentrations of PAHs in muscles of L. abu ranged between 2.301 and 16.661 ng/g dry weight during winter and summer respectively and in C. auratus between 1.095 and 8.675 ng/g dry weight during winter and summer respectively ,whereas in mollusc (P. euphraticus) ranged between 1.86 and 6.88 ng/g dry weight during spring and summer respectively. According to ratios of Low Polycyclic Aromatic Hydrocarbons(LPAHs) to High Polycyclic Aromatic Hydrocarbons (HPAHs), BaA/(BaA+Chr), InP/(InP+BghiP) and Fl/Py ,they certainly reflected that the PAHs sources in fishes and mollusk are pyrogenic as a main sources and petrogenic as a small part .Also results of this study revealed the presence of seasonal and spatial variations in concentrations of TPHs ,n - alkanes and PAHs in water ,sediments and aquatic biota from Al - Kahlaa River ,the highest concentrations were recorded during winter whereas the lowest during summer ,also the highest values of these compounds were recorded in Treatment unit station in comparison with other stations.

تقييم تلوث الهواء باستعمال دليل نوعية الهواء AQI ودليل التحول APTI لبعض المواقع المختارة في محافظة البصرة == Assessment of air pollution using AQI and APTI indices in selected locations of Basra governorate

Author name: شيماء هشام شهاب الدين
Supervisor name: نايف محسن عزيز
General topic: Biology
Specific topic: Environment - Environmental pollution
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study has been done for period from December to January 2015 including five station in three replicate for each station distributed in Basra Province (Al - Qurna district ,city center - Ashar ,Altenuma, and Zubair as well as region which is called Abu Al Khaseb , it is chosen as a control station for being agricultural area. The samples have been collected monthly for evaluating the parentage of gas pollution concentration (CO2,CO,NOx,SO2 and TSP) . The concentration of gas is measured by using gas analyzer device and the mount of particles in the air is measured by PDR - 1200. And black carbon ( BC) was measured by using Aethalometer . AQI was using for air quality for knowing the quality of surrounding air . Maps are drawn which show the spreading pollutants in Basra Province during the season by using GIS program . it is clear that there was differences concentration rates of pollutant gases in Basra province, the seasonally difference for gases pollutant proved that in Winter recorded high value for gases CO2, CO which reached 258.62 ppm for CO2 and 7.19 ppm for CO while in summer it is register the high value for NOx and SO2 which reached 0.406ppm for NOx and 0.319 for SO2 while TSP recorded difference reach high concentration 323.54 μg/m3 during Spring .While ,there is no difference has been recorded for black carbon in Autumn and Winter where as high concentration reached 25000 ng/m3 .The yearly difference in Al ashar shows that the highest for CO2 and CO during the period of studying followed by Al - Qurna recorded the highest concentration for NOx and SO2 while al - Zubair station recorded the highest concentration for TSP . Abu al Khaseb station recorded the lowest concentration for all gases and TSP because nature of the region which is formed from farms with lack of human activity .according to AQI which is use to measuringBthe air quality which shows the status of air and the degree of pollution , seasonally it is the highest rang for moderate in yellow color in Abu Khaseb station to unhealthy for sensitive groups in orange color in both al Zubair and Altenuma station where as al Qurna station recorded red color for unhealthy and in Al Ashar station they are record very unhealthy in purple color .while AQI for annually difference for the stations of study for unhealthy for sensitive groups in orange color for all stations except Abu Khaseb station , these groups are considered by negative affection human being health .Biochemical analysis has been done for leaves of plants that are spreading in the study area for knowing the effect of pollutants on biochemical indicators .The study included three kinds of plants which are phoenix dactylifera , ziziphus sp. And conocarpus lancifolius . Biochemical analysis test included total chlorophyll , water content, ascorbic acid and pH .the total chlorophyll estimated by using UV - spectrophotometer ,while ascorbic acid was estimated by using gas chromatography . The results shows significant different among leaves for different plants species in different study stations , the Date palm trees phoenix dactylifera recorded the highest concentration for total chlorophyll with the comparison with other plants. The seasonally differences show that lowest concentration of total chlorophyll in Winter reached 2.11 mg/g .The ascorbic acid recorded highest value in Winter reached 1.41 mg/ g and the water content rate of less recorded value in Spring reached %59.26 with no differences are recorded in the rest of the seasons . pH was recorded the highest rang in Winter reached 7.53.Ziziphus sp. recorded the lowest content of total chlorophyll for Winter reached 1.87 mg/ g and ascorbic acid recorded the highest value in Winter 1.31 mg/ g . whereas there was no significant different in water content in all seasons .pH recorded the high rang 6.64 in Winter , there were no significant in the rest of the season .Conocarpus lancifolius recorded the lowest value of total chlorophyll comparison with the other plants .while the lowest value for total chlorophyll recorded 1.42 mg/g in Winter . Ascorbic acid reached the highest concentration 1.31 mg/ g in Winter , while the water content recorded the lowest value in Winter 36 .88 % . While pH recorded 5.80 the lowest value in Winter.According to the biochemical analysis for plants for getting the value APTI for knowing the ability of tolerance of plants for air pollution which reflects the capability of the plants to tolerance of air pollution . The result of the study shows that Phoenix dactylifera recorded the highest value 7.36 in Al ashar station where as there are no significant recorded among the other station . Ziziphus sp. recorded the highest value 6.41 in al - Qurna station followed by al - Ashar station 6.07 whereas the other station did not recorded any significant . It has been noticed that Conocarpus lancifolius shows that lowest value of APTI which reached 3.91 in Al Zubair station and there were significant differences among the other stations.APTI was used for getting the value of Anticipated Pollution Index (API) which estimating biological characteristic and the economic value for plants. The results of API showed that the plants in Basra province Phoenix dactylifera recorded the value 6 and it was in excellent category. Ziziphus sp. recorded as poor plants recorded the value 2 . while Conocarpus lancifolius recorded the value 1 category as very poor plants.it has been noticed from values of APTI and API for phoenix dactylifera makes it typical plants as bioindicator and reducing the pollution .It is concluded for the current study that there are variation quantity and quality for gases and obvious spread for it is concentration of polluted gases and particles in air of the study stations in Basra. It was found that Phoenix dactylifera is a typical plants for using as bioindicator and reducing the air pollution .

خصائص خمائر البيئة النهرية لمحافظة البصرة وقابلية بعضها على المعالجة الحيوية للعناصر الثقيلة والهيدروكربونات النفطية == Characterization of yeasts in river habitat in Basrah Province and an ability of some of them for Bioremediation of the heavy metals and oil hydro carbonate

Author name: سناء قاسم بدر
Supervisor name: باسل عبد الزهرة عباس
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:

تاثير هرمون الثايروكسين على بعض المعايير الوظيفية للدم وبعض الجينات المنظمة لتمايز النسيج العظمي في الفئران المختبريةmusculus L. Mus. == Effect of Thyroxine hormone on some of physiological parameters of blood and some regular genes of bone differentiation in Laboratory Mice Mus musculus L.

Author name: سلمى سعيد عباس
Supervisor name: سامي جبر المالكي | علي عبد اللطيف العلي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: اجريت الدراسة الحالية لمعرفة تاثير هرمون الثايروكسين على تمايز العظم وبعض المعايير الفسلجية والتي شملت المعايير الدموية والهرمونات المحرضة للقند واوزان واطوال الاجنة واوزان المواليد في الفئران المختبرية البيضاء Mus musculus L.,اذ قسمت اناث الفئران المختبرية في الدراسة الحالية على مجموعتين هما : المجموعة الاولى الرئيسية : لدراسة تاثير هرمون الثايروكسين L.Thyroxine hormone على المعايير الدموية للاناث الحوامل وغير الحوامل وعلى تمايز العظم في الاجنة(باستخدام طريقة تحضير المقاطع النسجية الاعتيادية للاجنة والطريقة الجزيئية باستخلاص المادة الوراثية (RNA) من الاجنة وباستخدام تقنية النسخ العكسي RT - PCR والبلمرة الكمية qRT - PCR ومعرفة تاثيره على اوزان المواليد والاجنة, وتالفت من 90 انثى بالغة بعمر(10 - 12)اسبوع وقسمت على مجموعتين ثانوية ,و هي مجموعة السيطرة والتي حقنت حيواناتها بـ 0.1 مل من المحلول الفسيولوجي0.9% ولمدة 10 ايام متتالية.بينما المجموعة المعاملة حقنت حيواناتها بـ0.1 مل من هرمون الثايروكسين وبجرعة مقدارها 0.5 ملغم /كغم/يوم ولمدة 10 ايام يوميا بعدها شرحت 5 اناث من كلا المجموعتين ,و زوجت 40 انثى المتبقية ولكلا المجموعتين مع الاستمرار بالحقن لحين تشريح الاناث واستخراج الاجنة خلال فترات عمرية متباينة (9و10و11و12و13و14و15) يوم.اما المجموعة الثانية الرئيسية خصصت لدراسة تاثير هرمون الثايروكسين على الهرمونات المحرضة للقند وهرمونات الدرقية وانزيم Alkaline phosphatase.وتتالف من 15 انثى وقسمت على ثلاث مجاميع ثانوية تضم كل منها (5) اناث, وهذه المجاميع هي : مجموعة السيطرة وحقنت بـ0.1 مل من المحلول الفسيولوجي 0.9%.ومجموعة المعاملة الاولى وحقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 15 يوم للاناث العذارى قبل التزاوج.ومجموعة المعاملة الثانية حقنت بـ0.1 مل من محلول هرمون الثايروكسين وبجرعة 0.5 ملغ/كغم/يوميا ولمدة 30 يوم . اظهرت نتائج الدراسة الحالية ان المعايير الدموية قد تباينت قيمها خلال الفترات الزمنية وللمجموعات ضمن المدى الطبيعي عدا في حالات قليلة ,اذ وجد هناك انخفاض معنوي في اعداد خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية واعداد كريات الدم الحمر,وكذلك قيم مستوى كمية الهيموكلوبينHb والنسب المئوية لحجم الدم المضغوط PCV(HCT)% ومعدل كمية الهيموكلوبين MCHومعدل تركيز الهيموكلوبينMCHC ومعدل حجم كريات الدم الحمرMCV والصفيحات الدموية PLT في فترات متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وبينت النتائج وجود زيادة معنوية في خلايا الدم البيض والخلايا الحبيبية والخلايا الوحيدة والخلايا اللمفية والنسب المئوية للخلايا الحبيبية والوحيدة واللمفية, ومعدل تركيز الهيموكلوبينMCHC والنسبة المئوية لحجم الدم المضغوط %HCT ومعدل حجم كريات الدم الحمر MCVو الصفيحات الدميةPLT في مدد متباينة من الحمل للمجموعة المعاملة مقارنة مع مجموعة السيطرة. وسجلت نتائج الدراسة الحالية حصول ارتفاع معنوي في قيم هرموني T4,T3في الحيوانات المعاملة مع انخفاض معنوي في تركيز هرمون TSHفي الاناث المعاملة مقارنة مع السيطرة,و اوضحت النتائج ايضا انخفاضا معنويا في مستوى الهرمون اللوتيني LH وهرمون الحليب وللمجموعتين(15و30) يوم وزيادة معنوية في الهرمون المحفز للجريبات FSHللمجموعة الثانية(30 يوما) ,مقارنة مع مجموعة السيطرة, ولايوجد تاثير معنوي لهرمون الثايروكسين على هرمون الشحمون الخصوي ولنفس الفترتين مقارنة مع مجموعة السيطرة, وحصلت زيادة معنوية في مستوى انزيم الفوسفاتيز القاعدي بعد 30 يوما من الحقن بهرمون الثايروكسين مقارنة مع مجموعة السيطرة ,وحصول انخفاض في اوزان الاجنة بعمر 15يوما والمواليد للاناث المعاملة مقارنة مع مجموعة السيطرة , وبينت الدراسة زيادة معنوية في اطوال الاجنة المعاملة بعمر 9 و10و11و15يوما مقارنة مع مجموعة السيطرة. اما الدراسة النسجية فقد بينت ان الهيكل العظمي في الاجنة بعمر 9و10و11 يوما عبارة عن بداءات اعضاء تتكون من نسيج ميزنكيمي,ولم تسجل الدراسة النسجية وجود فرق بين مجموعة السيطرة والمعاملة في تمايز العظم .وظهرت بداية التعظم الغشائي والغضروفي في الاجنة بعمر12يوما ,اذ لوحظ بداية تمايز الخلايا الميزنكيمية الى ارومات عظمية,مع وجود القليل من المادة البينية الشبيهة بالعظم بين الارومات العظمية في منطقة الفك السفلي قرب غضروف ميكل ,والفك العلوي والعظم الحنكي واللامي وغضاريف الاضلاع ,اما في الاجنة بعمر13 و14و15يوما فقد ازدادت عمليات تمايز العظم بنوعية ,فاضافة الى النمو الذي حصل في العظام السابقة في تكوينها فقد ظهرت عظام جديدة في مناطق اخرى من هيكل الجنين تمثلت بعظام الصفيحة القاعدية والفخذ وحزام الكتف وحزام الحوض والاضلاع ,اما النتائج الجزيئية الخاصة بتمايز النسيج العظمي في اجنة الفئران المختبرية فقد اشارت الى التعبير الجيني لبعض جينات النسيج العظمي وتشمل Runx2وOSX وOsteo(OSC) وSialo(BSP) والتي بلغت ذروتها في مرحلة (13) يوم. ان لهرمون الثايروكسين تاثير على التعبير الجيني لبعض الجينات التي ترتبط مع التمايز النسجي للعظم اذ يؤدي هذا الى التغير في التعبير الجيني زيادة احيانا وانخفاضا خلال المدد العمرية المختلفة للاجنة .نستنتج من الدراسة الحالية ان زيادة مستوى هرمون الثايروكسين يسبب اضطراب في المعايير الدموية والتعبير الجيني لجينات العظم وانخفاض مستوى الهرمونات المحرضة للقند (LH وProlactin) وزيادة مستوى الهرمون المحفز للجريبات, وتعد هذه الدراسة الاولى على مستوى جامعة البصرة | the present study was performed to know the effect of thyroxine hormone on differentiation of bone tissue and some of physiological parameters, which included blood parameters and Gonadotropine hormones and Weights and Lengths and birth weight of mus musculus L.the females are divided into two groups ;the main first group which included 90 adult females (10 - 12)week, that subjected to study effect of thyroxine hormone on blood parameters of pregnant , nonpregnant females ,and bone differentiation of embryos (by using tissue sections method and molecular technique by RT PCR , and qRT PCR), and also known it is effect on weight of fetus and newborns; this group was also subdivided into two secondary subgroup : - control group ; was injected with 0.1 ml of normal saline (0.9%) for 10 consecutive days ,and treated group; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 10 consecutive days ,after that, 5 females from both groups were anatomized,while 40 females were mated of the both groups with continuous of injection until the females were subjected to anatomical practices and then the the embryo were taken during varied peroids (9,10,11,12,13,14,15) day. The main second group which included 15 females ,was selected to study the effect of thyroxine hormone on ;sexual hormone ,thyroid hormone and alkaline phosphatase . this group was divided into another 3 subgroups,one regarded as control group which ; was injected with 0.1 ml of normal saline (0.9%) for 15 consecutive days.and second the first treated group (T1) ; was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 15 consecutive days ,and without mating of the females,and the second treated group ; (T2);was injected with 0.1 ml of thyroxine hormone (0.5mg/kg/day) for 30 consecutive days and also without mating of the females. Results of the present study were shown that the blood parameters were varied in value between periods and groups (decreasing and increasing among normal value ) with exception in few cases , significant decrease of W.B.C , the granular cells ,monocytes, lymphocytes ,lymphocytes percentage ,granulocytes percentage and monocytes percentage during different periods of pregnancy in the treated animals compared with control , and was decreased RBC ,Hb ,HCT percentage ,MCH ,MHCH ,MCV, and PLT in varied periods , and we noticed a significant increase in WBC ,granular cells ,monocytes ,lymphocytes , granulocytes percentage , monocytes percentage , MCHC ,HCT percentage,MCV, and PLT in varied periods of pregnancy in the treated animals compared with control. Our study was exhibited significant increase in levels of T3 and T4 hormones of the treated animals compared with control, with significant decrease in TSH of treated animals , also significant decrease in LH and prolactin H and for groups(15 and 30 days) ,and significant increasing in FSH of second group (30 day) ,when compared with the control , and there was no effect of thyroxine on TEST H level and for 2 periods(15 and 30) , significant increasing in alkaline pho after 30 days from injection with TH H , decreased of body weight of embryos (15 days) and newborns of the treated females ,the current study showed significant increase of lengths of treated embryos (9,10,11,15 days). The histological study was explained the skeletal tissue ossification of embryos during (9,10,11)day post gestation was primitive organs of mesenchymal tissue and The histological study was not show asignificant differences of bone differentiation between treated and control groups,and intramemberaneous ossification was appeared of embryos at 12 days ,in this stage the begining of differentiation of mesenchyme cells was appeared into osteoblast , with a little of matrix like bone between osteoblasts in Mandible near the meckle cartilage , Maxilla, Palatine,and Hyoid, and endochondral ossification as in Ribs, where in embryos (13,14,and 15) The bone differentiation processes were increased of bone (the intramembranous ossification and endochondral ossification),in addition to the growth of previously formed bones,also new bones were appeared in different areas in embryo skeleton like Basal plate,femur,Scapula girdle , pelvis girdle and the Ribs . In other hand, the molecular results of bone differentiation in embryo ,were exhibited peak of expression of some of the genes of bone tissue (Runx2 ,OSX, Osteo(OSC) ,and Sialo(BSP) ) at 13 days of embryo age, also the thyroxine hormone was effected in expression of some the bone genes that included disturbanceas like increasing of expression during peroids and decreasing in other peroids of embryos life. We concluded from the present study that the increase of thyroxine hormone level caused disturbances of blood parameters and gene expression of the bone genes & decreasing of the level of LH & prolactin hormones and increase the level of FSH. This is the first study on the Basrah University

دراسة مقارنة لتاثير هرمون النمو في عضلات وCyprinus مناسل سمكتي الكارب الاعتيادي والبلطي احمر البطن carpio (L. 1758) Tilapia zillii (Gervais, 1848 == A Comparative Study to the Effect of Growth Hormone on Muscles and Gonads of Carp Cyprinus carpio (L. 1758) and Tilapia Tilapia zillii (Gervais, 1848)

Author name: سعد محمد صالح عبد الصمد
Supervisor name: عقيل جميل منصور | عبد الكريم طاهر يسر
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: In the present study, the effect of human growth hormone on the muscles and gonads of common carp Cyprinus carpio (L. 1758) and red belly tilapia Tilapia zillii (Gervais, 1848) was studied by : - 1 - Studying the effect of human growth hormone on the growth of the two studied species by intraperitoneal (IP) injection of three replicates of control with 120 μg of normal saline / g Body weight and two doses (low and high) with 60 and 120 μg of human growth hormone / g Body weight respectively for both species, then changes were followed up in each treatment after every two weeks of injection by : - a - Pursue histological changes in the muscular tissue by using Cell Profiler software for the first time in Iraq. b - Pursue changes in chemical components ratios of body muscles.c - Measuring of specific growth rate and length - weight relationship for fishes.2 - Studying of human growth hormone effect on the gonads of fishes (adult males and females) with 60 males for each species and a same number of females, all of them injected either with low or high dose with 60 or 120 μg of human growth hormone / g Body weight respectively for both species or with 120 μg of normal saline / g Body weight in fishes of control. This experiment had achieved in a month.3 - Knowing the direct effect of human growth hormone on the follicles by implementing an in vitro experiment with the follicles of carp which implanted in basic salt solution (BBS) then growth hormone was added to the test tubes with two concentrations 5 μg/ ml and 10 μg/ ml, the control follicles were implanted in normal saline. The results of the study revealed that the maximum rate of percentages of the muscle fibers with area less than 314 μm2 for region R1(Dorsal side) was 88.29 % in carp in high dose treatment after the eighth week of injection, while it was 88.00 % for tilapia in high dose treatment after the eighth week of injection, too.In the region R2 (in tail) the maximum rate of percentages of muscle fibers with area less than 314 μm2 was recorded after the eighth week from the beginning of injection was 73.3553 % for carp in high dose treatment and 85.935% for tilapia in high dose treatment.The results showed that 1.00984 and 1.000074 were the highest and the lowest values of condition factor for carp respectively. For tilapia those were 1.009993 and 1.000151 the highest and the lowest values of it respectively.The rate of specific growth rate for length was 0.3616 and 0.44866 cm/day% for carp and tilapia respectively. While the specific growth rates for weight were higher than the comparable one for length and were 1.3489 g/day% for carp and 1.6085 g/day% for tilapia.The results exhibited that the rates of protein percentages for both species in the all treatments in general tend to be higher with progressing of experimental time. Muscle water content percentage rate varied with difference of dose and experimental time. The maximum rate of fat percentage in both species was in control treatment after two weeks of hormone administration.The highest rate of carp male Gonadosomatic index was 6.55% in high dose treatment after the third week of injection, as for tilapia males was 6.103% in low dose treatment after the third week of injection, while the lowest value for this index was recorded after the first week of injection in high dose treatment which as 2.81 and 2.89% for carp and tilapia respectively. For females the highest rate of Gonadosomatic index was in high dose treatment after the third week of injection for both species which was 11.001 and 6.0624% for carp and tilapia respectively. As for the lowest rate of carp female Gonadosomatic index was recorded in control treatment after the first week of injection it was 5.54%, while the lowest rate of this index for tilapia females was 2.99% in low dose treatment after the first week of injection.In the experiment of direct effect of this hormone on the follicles of carp it was found that the highest value of polarization index was 4.12 which recorded in high dose treatment after 48 hours of incubation while it reached the lowest value which was 3.031 in high dose treatment after 24 hours ofincubation.

دراسة تصنيفية وحياتية للفطريات الناقصة الملونة في البصرة == Taxonomical and Biological study of Dematiaceous imperfect - fungi in Basrah

Author name: زينب خلف عبد الله
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: During this study 23 species belong to 10 genera of imperfect fungi were isolated from different natural sources ( plants, soil , and ari) from Basrah govermanete using different isolation methods.Among the isolated fungi the genus Alternaria was selected to identify its species by using the morphological characteristis by light microscopy and Scaning Electron in Microscope (SEM) and verified by molecular and chemical methods. Eight species of Alternaria were identified namely; A.alternata (isolate1 and 2) A.chlamydospora A.citri, A.raphani , A.longipes , A.infectoria A.tenuissima and A.chrysanthemi based on light microscope examination.Taxonomic relegation of these species were confirmed by SEM image. SEM revealed that there was differences in the conidial wall ornamentation .The molecular analysis using PCR depending on the loci ITS1, and ITS4 700bp which confirmed the morphological identification of most of species namely A.alternata (isolate 1 and 2) , A.chlamydospora , A.longipes , A.infectroia and A.tenuissima. However , A.citri was related to A.alternata, A.raphani was belonged to A.infectoria and A.chrysanthemi is belonging to A.lolii. Nevertheless ,by using the loci OPA1 - 3 there was no variation among the species of Alternaria ,this is may be due to the inadequate method applied.The genetic variation of 36 isolates of A.alternata was studied using 9 - primers by RAPD - PCR technique. All primes gave an amplification of DNA for all the fungal isolates. Among the primers OPC08 exhibited a highest efficacy reaching 235 total bands .In the present study the antibacterial activity of fungal crude extract was tested. It appeared that the extract exhibit an antibacterial bioactivity against E.coli , S.aureus , S.epidermidis and S.saprophyticus.However, the extract of A.alternata (isolate 1 and 2) showed an inhibition growth against all the tested bacterial strains. This study was also revealed that the fungal filtrates of Alternaria species possessed various chemical compounds. However, there were differences in the chemical composition among the fungal filtrates.The results showed that Alternaria species ( A.alternata isolate 1 and 2, A.chlamydospora A.citri, A.raphani , A.longipes) were capable to biosynthesize silver nanoparticles, when the fungal filtrate were treated with AgNO3 solution after72 hrs incubation .Silver nanoparticles synthesis was checked by the change of colour from yellow to brown and confirmed by Uv - vis spectrophotometry at wave length (420, 440 nm). SEM image showed that the silver nanoparticles with spherical shape and size ranged between (20 - 72nm). Aconclusion can be derived that application of SEM,chemical analysis of fungi extracts ,molecular analysis and the silver nanoparticles characteristics are useful in the taxonomy of fungi to solve the overlapping of the morphological identification .Taxonomic key were prposed for the species of the genes Alternaria based on the morphology , chemical compound with the fungal extracts and size of silver nanoparticles synthesized by fungi .Melanin pigment identify by TEM microscope and it appeare like dark drop inside the cell .

دراسة مجتمعات اللافقريات القاعية الكبيرة في ثلاث بيئات مائية مختلفة في جنوب العراق == Synecology of Macrobenthic Invertebrates of Three Different Aquatic Habitat at Southern Iraq

Author name: رغد زيدان خلف
Supervisor name: هيفاء علي حمزة | مرتضى يوسف العباد
General topic: Biology
Specific topic: Zoology - Invertebrates
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study aimed macrobenthic invertebrats communites in three different aquatic environments southern Iraq. Eight stations were chosen for this purpose, Four of them distinction by being with running water and affected with the tide of Arabian Gulf,these include two stations on each of Shatt Al - Arab River (Al - Mohamadiat and Al - Salihia) and east part of Al - Hammar marsh (Al - Burgah and multaqaa Al - Mashab and Al - Sallal).the other four stations were not affected with the tide and located on the central marshes, two stations on each of Aum Al - Showaich marsh (Al - Sibitia and Al - Badria) and Al - Chebaeish marsh (Abu Sobat and Al - Sihaqee).The samples of water and sediment were collected monthly during the period from April 2014 to March 2015.Some environmental factors for each station were measured these include air and water temperatures, pH ,electrical conductivity, turbidity, dissolved oxygen, biological oxygen demand, total dissolved soild, total hardness, Calcium, nitrate , reactive phosphate, sulphate, total organic carbon and soil texture. The macrobenthic invertebrates samples which collected by Three replicates monthly for each station were isolated and identified,then densities and relative abundance were calculated.Some ecological indices such as diversity, richness, evenness, dominance, beta diversity and similarity indices(qualitative and numerical) were studied. Canonical correspondence analysis was adopted to evaluate the effect of the measured environmental factors on the occurrence and density of the studied macrobenthic invertebrates.The results showed that the variables of the stations were ranged as below : Air temperature (12 - 44)C̊ ,while the water temperatures (10.6 - 35)C̊ , PH (7.5 - 9.1), EC (2.07 - 13.7) mS /cm, Turbidity (4.2 - 199) NTU, DO (5 - 11.5) mg/l , BOD5 (0.3 - 3.5)mg/l , TDS(1245 - 10220)mg/l , TH (531 - 2928) mg/l , Ca+2 (80.2 - 320)mg/l , NO3 - (0.26 - 15.25) mg/l , PO4 - 3 (0.032 - 2.9) mg/l , SO4 - 2 (250 - 910)mg/l , and for sediments : TOC (9.3 - 22.5)% and soil texture ranged between sandy silt to muddy silt.The total number of recorded taxes were 80,representing by 16 species belonging to Annalida, 36 genus to the Aquatic insects (28 genus of them were first record in the middle wetlands), 16 species to Crustacea and 12 species to Mullosca. The species Pomacea canaliculata was recorded for the first time from Shatt Al - Arab river. The recorded taxes were classified according to its occurrence periods into four classes : dominant, fluctuated, less abundant and rare.The monthly density of each taxa of macrobenthic invertebrate and for each main macrobenthic invertebrate group in all stations was calculated. The results revealed that the densities were ranged from( 0 - 7115, 0 - 485 , 0 - 703 and 0 - 1199) ind/m2 for the groups of annelid, aquatic insect, crustacean and mollusca respectively. While the total density of all macrobenthic invertebrate groups was ranged between( 0 - 7367) ind/m2.The results of annual relative abundance for each main macrobenthic invertebrate group in all study stations referred that the value 66.3% was recorded for the annelids, while the lowest one 6.1% was recorded for the aquatic insects. On the other hand, the annual relative abundance for each main group in each station was investigated, and was ranged between (0.9 - 68)% recorded in Shatt Al - Arab river stations for the groups of aquatic insects and annelids respectively,( 0.4 - 67)% recorded in east Hammar marsh stations for groups of water insects and mollusks respectively, and (1 - 82)% recorded in Middle marshes stations for the annelids. The annual relative abundance for total macrobenthic invertebrate group in all stations was ranged from (2 - 27)% recorded in Al - Sibitia and Al - Sihaqee stations respectively. The annual relative abundance for the species and genus which recorded during our study revealedthat all highest values (94, 96.58 and 100)% were recorded for the species Limnodrilus hoffmeisteri in stations of Shatt Al - Arab River, east Hammar marsh, and Middle marshes respectively.The monthly relative abundance for main macrobenthic invertebrate groups in all station was studied. The highest value which recorded from Shatt Al - Arab river stations (Al - Salihia station) was reached 95.8% and recorded for the annelids, while reached 100% either in east Hammar marsh stations(Al - Burgah) for the mollusks and in Middle marshes stations (Al - Sibitia) for the crustaceans.Some ecological indices for each for each main macrobenthic invertebrate group from all stations totally were calculated. About the spatial diversity the range of Shannon - Wiener diversity index were between 0 - 0.6674 for annelids, 0.8718 - 1.92 for aquatic insects,0.5404 - 1.917 for crustaceans and 0.3035 - 1.15 for mollusks, while were 1.076 - 2.085 for all the benthic groups totally.The values of Richness index were from 0 - 1.127 for annelids,0.778 - 3.683 for aquatic insects, 0.5212 - 2.104 for crustaceans and 0.2543 - 1.277 for mollusks, while were 2.321 - 5.225 for all the benthic groups totally. The values of Evenness index were ranged between 0 - 0.9629 for annelids,0.4649 - 0.7419 for aquatic insects ,0.3016 - 0.8327 for crustaceans, and were 0.2189 - 0.8974 for mollusks, while were 0.3121 - 0.77 for all benthic groups totally. The values of Dominance index were ranged from 0.6129 - 1 for annelids, 0.343 - 0.7736 for aquatic insects ,0.2991 - 0.8772 for crustaceans,0.5075 - 0.9845 for mollusks while were 0.2638 - 0.7762 for all benthic groups totally.About the temporal diversity the values of Shannon - Wiener diversity index were between 0 - 0.99 for annelids, 0 - 2.01 for aquatic insects, 0 - 1.5 for crustaceans and 0 - 1.637 for mollusks, while were 0 - 1.981 for all benthic groups totally. The values of Richness index were from 0 - 0.969 for annelids, 0 - 2.717 for aquatic insects, 0 - 1.314 for crustaceans and 0 - 731 for mollusks, whilewere 0 - 3.047 for all the benthic groups totally. The values of Evenness index were ranged between 0 - 1 for each of annelids, aquatic insects and crustaceans, and were 0 - 0.992 for mollusks, while were 0 - 971 for all benthic groups totally. The values of Dominance index were ranged from 0 - 1 for each of four benthic groups, while were 0.3 - 1 for all benthic groups totally.Whittaker Beta index between the three different aquatic habitats for each main macrobenthic invertebrate group was calculated. The lowest value 1.14 was recorded for the mollusks between the middle marsh of um Al - Shoowaich and Al - Chebaeish, and highest value 1.66 was recorded for the aquatic insects between the east Hammar marsh and marsh of Al - Chebaeish, while the index values for all groups of macrobenthic invertebrate totally were ranged from 1.33 between the Shatt Al - Arab river and east Hammar marsh to 1.54 between the Shatt Al - Arab river and marsh of Al - Chebaeish.Jaccard Similarity index for each main macrobenthic invertebrate group for each stations was calculated.The values of index for annelids were ranged from( 0 - 89)% the lowest value recorded between Al - Burgah and Al - Sibitia stations and the highest value between Al - Salihia and Multaqaa of Al - Mashab and Al - Sallal stations, for the aquatic insects the values of index between (8.3 - 46.7)% the lowest value between Al - Salihia and Al - Sibitia and the highest value between Al - Mohammediat and Al - Badria stations, for the crustaeceans values ranged(14.3 - 77.8)% the lowest value between Al - Mohammediat and Al - Badria stations while the highest value between Al - Sibitia and Al - Badria,and for mollusks were between(18 - 80)% the lowest value between Al - Mohammediat and Al - Burgah stations while the highest value between Multaqaa of Al - Mashab and Al - Sallal and Abo Sobat stations.The index for all macrobenthic invertebrate groups totally also calculated and its values were from (15 - 58.5)% recorded between Al - Mohammediat with Al - Sibitia stations and between Al - Mohammediat with Al - Salihia stations respectively.Bray - Curtis index was calculated monthly for each main macrobenthic invertebrate group in each stations .Also the Bray - Curtis index was calculated annually between stations and the results refer that the highest values as the following : 0.993 for the annelids between Al - Badria and Al - Sihaqee stations, 1 for the crustaceans between Al - Salihia and Al - Sibitia stations, and 0.96 for the mollusks between Al - Sibitia and Abo Sobat stations.The highest value for all benthic group totally was 0.983 between Al - Salihia and Abo Sobat stations.On other hand Bray - Curtis index was calculated for all macrobenthic invertebrate groups totally between the three aquatic habitats and the values were ranged from 0.5 recorded between the wetlands of um Al - Shoowaich and Al - Chebaeish to 0.875 recorded between the east Hammar marsh and wetlands of um Al - Shoowaich.The Canonical Correspondence Analysis (CCA) by using Canoco application revealed the clear effect of different ecological factors on numbers of the individuals and species of each group of macrobenthic invertebrate in all stations during the study period.

الكساء الخضري والتنوع الاحيائي النباتي في منطقة الصحراء الجنوبية في محافظة البصرة / جنوب العراق == Vegetation and Plant Biodiversity in the Southern Desert in Basra Governorate, Southern Iraq

Author name: حيدر راضي مالح حسين
Supervisor name: عبد الرضا اكبر علوان المياح
General topic: Biology
Specific topic: Plant - Environment
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: تناولت الدراسة الحالية الغطاء النباتي والتنوع الاحيائي والمجتمعات النباتية في منطقة الصحراء الجنوبية ضمن محافظة البصرة, وتم دراسة الخصائص الكمية والنوعية للغطاء النباتي , فضلا عن خصائص الترب والهواء في 12 محطة, 6 منها خصصت للمسح الشهري للانواع للفترة بين كانون الاول 2012 وكانون الاول 2014.تم دراسة الخصائص الفيزيائية والكيميائية للعوامل البيئية في منطقة الدراسة وشملت حساب قيم درجة حرارة الهواء والرطوبة النسبية, وكذلك دراسة خصائص الترب ومنها قوام التربة ودرجة حرارة التربة ورطوبة الترب والاس الهيدروجيني والمادة العضوية والتوصيلية الكهربائية وقيم الملوحة, وتبين انها اظهرت تباينا موسميا في المحطات الستة. اوضحت الدراسة الحالية وجود 185 نوع و136 جنس تعود الى 41 عائلة, منها 159 نوع و115 جنس و36 عائلة تعود الى مجموعة نباتات ذوات الفلقتين, في حين ضمت مجموعة نباتات ذوات الفلقة الواحدة 26 نوع تقع في 21 جنس و5 عائلات, وكانت اغلبها نباتات حولية حوالي 66% , بينما كانت نسبة النباتات المعمرة 44 %, كما تم تسجيل حوالي 20 نوع لاول مره في مناطق الدراسة, كما اكدت التوزيع الجغرافي وسجلت انتشار العديد من الانواع في مناطق لم تسجل فيها سابقا.وبينت دراسة اشكال الحياة في منطقة الدراسة ان مجموعة Therophytes قد امتلكت اعلى نسبة بلغت 71.19%, بينما ضمت مجموعة ال Geophytes اقل نسبة بلغت 4.3 %. كما تغايرت الاصول الجغرافية النباتية Chorotype للانواع,عكست اهمية الموقع الجغرافي لمنطقة الدراسة, وسجلت العناصر ثنائية وعديدة المناطقBi & Pluri regional 70.7 elements % منها, وشكلت العناصر الصحراوية - العربية Sahara - Arabia elements نسبة 20.54%.وبينت النتائج ان اكبر العائلات انتشارا وتوزيعا وبعدد الانواع في منطقة الدراسة هي العائلة المركبة Compositae التي تعد اكبر العائلات في المنطقة, ثم العائلة الصليبية Cruciferae , والعائلة النجيلية Gramineae , والعائلة البقوليةFabaaceae , والعائلة الرمرامية Chenopodiaceae , كما تبين ان الاجناس Astragalus وPlantago وErodium كانت اكبر الاجناس بعدد الانواع في منطقة الدراسة, واظهر ت دراسة التوزيع الجغرافي ان بعض الانواع سجلت انتشارا واسعا في مناطق الدراسة ومنها Hammada salicornica Moq. وRhanterium epapposum Oliv.و Stipa capensis Thunb. وIflago spicata (Forssk) Sch. Bip. وFilago pyramidata L. وSpegularia diandra (Guss.) Heldr & Sart. وStrigosella grandiflora ( Bunge.) Boch. .كما تضمنت الدراسة وصف وتمييز المجتمعات الرئيسة وتحديد مناطق انتشارها في مناطق الدراسة وضمت Hammadetum salicornici وCornulacetum auchraeو Rhanterietum epapposi وZygophyllietum propinqun وAstragalietum spinosi وSalsoletum setiferaeو Convolvulietum oxyphylliو Salsoletum rosmarini وStipetum capensiو Ziziphetum nummulariaeو Teucriutum oliveriو Stipagrosis plumosi وLycetum barburae وSuaedetum aegyptiaci . واظهرت الدراسة التغيرات الموسمية في طبيعة الغطاء النباتي وتاثر السيادة بالتغيرات الموسمية في الظروف المناخية والتي ساهمت في ظهور المنطقة بعدد من المظاهر الموسمية للغطاء الخضري ( Seasonal Aspect) . وبينت نتائج الدراسة الحالية وجود تباين موسمي ومكاني بين غالبية محطات الدراسة في قيم ادلة الكثافة والوفرة والتغطية والتنوع والسيادة والتجانس والغنى النوعي والتشابه والكتلة الحية والتصاحب والاهمية.

دراسة جزيئية لتعدد الاشكال الوراثية لجين TAS2R38 (جين تذوق مادة PTC) بين سكان محافظة البصرة وعلاقتها بمرض السكري == A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes Mellitus

Author name: حسنة عامر مهوس
Supervisor name: فائزة عبد الوهاب احمد | اسعد يحيى عايد
General topic: Biology
Specific topic: Microbiology - Genetics
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: This study dealt with the genetic polymorphism of Phenylthiocarbamide (PTC) tasting from phenotypic and genotypic (Molecular of TAS2R38(P49A) gene ) prospective,among sample of Basrah Population from 2014 - 2012. Also, a comparative Molecular study for the gene TAS2R38(P49A) and some biochemical parameters was made in a group of Diabetic patients for the first time in Iraq /Basrah and the world .Phenotypic study using the gradient dilutions of PTC substance method, was established among 317 individual : 96Males and 221 Females from different regions of Basrah. Increased sensitivity with low threshold 6 of Males than Females 5 was observed which indicate that the Males were more sensitive than Females in this region from Iraq .However, the tasters percent among Females still higher73.3% than Males72.9% ,and the whole population 73.2% as well .This study recorded different tasters percent in different regions : 62.1%,71.8% ,60.6%, 82.3% for the North ,AL - Zubair and Safwan, Abu - ALChaseab and AL - Fao and the center of the city respectively, although ,the whole population of Basrah found to be under Hardy - Weinberg Equilibrium . Allelic frequencies according to phenotypic analysis showed : recessive allele t had higher frequency (0.53) than dominant allele T (0.47), with elevated Heterozygous frequency Tt among all studied regions and the whole population(0.53) .The results recorded also , high BMI for non tasters tt as a whole and for both sexes in this phenotype : 28.32 ,28.26 for males and females respectively. While it had the lowest value among heterozygous 23.40 ,Statistical analysis for phenotypic study of this locus reveald an additive effect of the two alleles T,t by 81.33% with responsibility of 83.10% for the phenotypic variation found in this trait . Genetic (Molecular) analysis was done for comparative individuals and diabetic patients by sequencing the hTAS2R38(P49A) the PTC gene, among 127 individual : 59 healthy individual as a comparative group and 68 diabetic patients from both sexes and same age ranges. The results recorded a C/G allele in the position of the first SNP (145 C/G) which encode for Proline amino acid (C) or Alanine amino acid (G) : CC , CG refer to Tasters genotypes while GG refer to Non - tasters genotype. High frequency of C allele as compare to G among both healthy people and diabetic patients : 0.55, 0.53 for both respectively . Frequency of heterozygous genotype CG recorded a significant access as 0.76 among the comparative group which may implies a selective heterozygosity advantage among Iraqi population at this region. A significant differences in genotypic distributions was recorded between comparative and diabetic groups with high incidence of non tasters phenotypically tt 41% and genotypically GG 20.5 % among diabetic patients compare to healthy people : 25 % , 7% respectively . Interestingly non taster GG genotype had the lowest BMI among diabetic patients compare to othertwo taster genotypes CC, CG respectively. . Study of Genotype - Phenotype Association revealed significant correlation (p<0.0001) between them among comparative group as 86.9% and diabetic group as 48.8%. A comparative study of Biochemical analysis betweendiabetic and non diabetic individuals from different genotypes for TAS2R38 (P49A) gene showed significant elevated value of cholesterol among diabetic from the GG genotype 191.85 ± 6.57 mg/dl with non significant elevated values of Hb1c 8.93 ±1.77 and triglyceride 149.57±6.53 mg/dl. However, all these parameters were significantly more higher among diabetic than non diabetic . Significant differences(P<0.05) were recorded between diabetic and non diabetic according to their thyroid hormones levels with low values of T3 , T4 and high values of TSH among diabetic than non - diabetic. TSH hormone recorded its highest significant values among diabetic of CC genotype with 4.39 ± 0.16 μUI/ml as compare to the other two genotypes heterozygous CG and recessive homozygous GG . Although non significant , T4 recorded its lowest values among diabetic of the CC genotype .However, significantly (P <0.05 ),T3 hormone found to have lower values in the two homozygous genotypes; CC genotype for diabetic 0.54± 0.03 ng/ml and non diabetic 0.53±1.64 ng/ml ; GG genotype : 0.72± 0.04 ng/ml ; 1.82± 0.59 ng/ml for diabetic and non diabetic respectively . while individuals with CG genotype found to be non significantly different .Lastly it could be concluded that this trait may have some effect on BMI and may play a role the variation found among peoples in biochemical parameters (cholesterol, thyroid hormones ) changing's especially among diabetic patients. Ministry of High Education and Scientific Research Basrah University/ College of Education for Pure Science Department of Biology A Molecular Study of TAS2R38 (PTC Tasting Gene) Polymorphism Among Basrah Population and It's Association With Diabetes MellitusA Thesis submitted to the Council of the College of Education for Pure Science/ University of Basrah as a partial fulfillment of the requirements for the degree of Philosophy of Doctor of Science in Biology / Genetics By Hasna Amir Mohaus BSc. Biology / 1994MSc. Biology / 1998 Supervision by Assist.Prof.Dr. Prof.Dr.Faizah A.

دراسة بعض التغيرات الفسيولوجية والنسجية لسم الافعى ذات الحراشف المنشارية (افعى سيد دخيل Echis carinatus) في الجرذان المختبرية == A study of some physiological and histological effects of venom Saw Scaled Viper (Said Dakhil) Echis carinatus in laboratory rats

Author name: بيداء ريحان علي الموسوي
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was designed search of some physiological and histological effects of the venom of Echis carinatus sochureki (Said Dakhil snake) in male and female rats, and the role of antivenom (anti - venom) in the treatment of these effects. The while study was divided into two parts, the first part included the effect of [0.04 mg/kg (the second group) and 0.08 mg/kg (the third group)] of venom in rats. While, the second part related with the effect of [0.25ml/animal (the second group) and 0.5ml/animal (the third group)] antivenom in male and female rats treated with 0.04mg/kg and 0.08mg/kg of venom. After 24 hours of injection, blood samples collected for the hematological and biochemical parameters, and taking the right epididymis and epididymis left removed to examine the count and maliformations of sperm. Also, liver, kidney, testes and ovary were collected for sections. The results of the present study showed a significant decrease (P≤0.05) in RBC in the third group (0.08) mg / kg when compared with the first group (control) and second group (0.04) mg / kg. Also was there a significant decrease in HCT and MCV in the second and third groups compared with the first group, while there was a significant increase in the platelets of the third group compared with the first and second groups in in both sexes. The results indicated a significant decrease in RBC in the second and third groups compared with the first group, also a significant decrease in HCT in the second and third groups compared with the first group. The results showed a significant increase in the HCT in the second group compared with the third group. The results showed a significant decrease in the MCV in the third gr oup compared with first and second groups in female rats. The present study (P≤0.05) showed a significantdecrease in total WBC, lymphocytes and neutrophils in the second groups (0.04) mg / kg and the third (0.08) mg / kg, and a significant decrease in monocytes, acidiophils and basophils of the second and third groups compared with the first in the male rats. The results indicated a significant decrease in neutrophil in the second and third groups compared with the first group, and a significant decrease in lymphocytes, monocytes and acidiophils of the third group compared with the first and second groups in the female rats. The present study showed a significant increase (P≤0.05) in the level of glucose and urea in the third group (0.08) mg / kg compared with the first (control) and second (0.04) mg / kg groups. There was a significant increase in creatinine and albumin in the second and third groups compared with the first group, also a significant decrease in the total protein, cholesterol and HDL in the second and third groups compared with the first group.There was a significant decrease in the level of TG, LDL and VLDL in the third group compared with the first and second groups, The results showed a significant decrease in ALT and AST in the third group compared with the first and second groups, and a significant increase in the ALP in the third group compared with the first and second groups in the male. The hormonal study showed a significant decrease (P≤0.05) in the level of FSH, LH, testosterone and estrogen in the second and third groups compared with the first group in male and female rats. The present study a significant decrease (P≤0.05) in the count of sperms in the second and third groups compared with the first group, while, there was a significant increase in the maliformations of sperms in the third group compared with the first and second groups. The present study showed a significant decrease (P≤0.05) in the level of immune globulins (IgA - IgG - IgM) in the second and third groups compared with the first group in both sexes. The results of the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated with (0.04) of snake venom showed a significant decrease in RBC and HCT in the second and third groups compared with the first groups,and there was a significant increase in the MCV in the second and third groups compared with the first group in male. The study results showed a significant increase in RBC, HCT and MCV in the second and third groups compared with the first group in the female rats.The current study showed a significant decrease (P≤0.05) in total WBC of the second group compared with the first and third groups, and a significant decrease in the lymphocytes and monocytes in the second and third groups compared with the first group, also the results showed a significant increase in total WBC in the second and third groups compared with the first in the male. Also, found a significant decrease in lymphocytes and monocytes in the secon d and third groups compared with the first, it was observed a significant rise in monocyte in the first group compared with the second and third groups. The results showed a significant increase in the percentage of granular white blood cells in the second and third groups compared with the first group in the female rats.Results of the present study indicated a significant decrease (P≤0.05) in the level of glucose, urea, albumin and total protein in the third group compared with the first and second groups, and there was a significant increase in the level of cholesterol HDL - VLDL in the third group compared with the first and second groups, also a significant decrease in TG in the second group compared with the first and third groups, The results showed a significant decrease in the level of HDL in the second and third groups compared and second groups with the firstgroup. the results showed a significant increase in the level of albumin and total protein in the third group compared with the first and second groups, and the results indicated a significant in increase cholesterol level, LDL and VLDL in the third group compare with the first and second groups, There was a significant decrease in the level of TG in second group compared with the first and third groups, and a significant decrease in the level of HDL in the second and the third groups compared with the first group in the female rats.The results indicated the effect of (0.5 - 0.25) ml / animal antivenom in male and female rats treated (0.08) mg / kg of snake venom, showed a significant decreased (P≤0.05) in RBC, HCT , MCV, lymphocyte and monocyte in the second and third groups compared with the first group, a significant decrease MCH in the second group compared with the first and third groups in male rats, showed a significant decrease in RBC HCT and MCV in the second and thirdgroups compared with the first group.The present study showed a significant decrease (P≤0.05) in the level ofglucose and urea, ALT and ALP in the third group compared with the first and second groups, also a significant decrease in the level of creatinine, LDL, VLDLand AST in the second and third groups compared with the first group, and asignificant increase in the level of albumin, total protein and HDL in the thirdgroup compared with the first and second groups. a significant increase in levelof cholesterol and TG in the second and third groups compared with the firstgroup in the male rats, While showed a significant decrease in level of glucose,urea and ALT in the third group compared with the first and second groups,there was a significant decrease in the level of creatinine, LDL, VLDL, AST and ALP in the second and third groups compared with the first group, a significant increase in albumin and total protein in the group third compared with the first and second groups, while a significant increase in level of cholesterol, TG and HDL in the second and third groups compared with the first group in female.The histological results showed changes in the liver of male and female rats treatment I n venom snake and constrictions consisted obtaining inflammation and blood congestion and enlarged nuclei of hepatic cells and degeneration in the cytoplasm and necrosis of liver cells, as well as, changes in the kidney tissue such as having a bleeding and hyperplasia and congestion blood and inflammation and analyze the entire glomerulus and necrosis of the cortical cells.The current study showed the incidence and clear changes in testicular tissue treatment venom snake of obtaining vascular congestion and analyze the interconnection fabric between the tubule and the decay of some nuclei and abnormalities tubule and testicular and shrinking and analyzes and the presence of multiple nuclei giant cells in the wall of tubule.The current study for histological changes in the ovary as sections showed the presence of large numbers of corpus luteum and the decrease numbers of ovarian follicles and the disappearance of the evolutionary stages of ovarian cysts.

تاثير PTU على بعض الجوانب النسيجية والفسلجية في الاجنة والاناث الحوامل Rattus norvegicus == Effect of PTU on some aspects of histological and physiological in fetus and pregnant rats(rattus norvegicus

Author name: انوار ناذر صيوان
Supervisor name: مها خليل الملاك | علاء عبد الخالق حسين
General topic: Biology
Specific topic: Zoology - Histology
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: Aim of study was to evaluated the effects of propylthiouracil (Ptu) drug on both maternal and fetus rats, used experimental animals (Rattus norvegicus) and after housing, breeding and adapting, (50) of virgin females choosen, isolated and then divided into two groups each group contain (25)rats, The mean weight of the animals was(200 ±50) gm and (8 - 10) week of age. Females on first group regarded as control and treated with distilled water while animals in second group were treated with (Ptu)dose as (0.05 %) and left for(14.5, 16.5, 18.5, 20.5, 21.5)for each group. Macroscopic observations on each group were recorded including changes in thyroid gland morphology, hypertrophy and enlargement at each period (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation for both pregnant rats and embryos,the shape of uterine hornes,and uterus with changes of fetus number, resorption, atrophy, malformation of body parts, indifferentiation of fore and hind limbs, skin abnormalities were noticed. The changes in placenta which appeared irregular, pale colour, congested with white fibrous regions were noticed in hypothyroid rat comparing with placenta of control group animal, which looked red, discoid shape and normal surface without any abnormal signs, in addition fatty ovaries with large amount of adipose tissue was accumulated in abdomen cavity, the ovaries irregular and showed structures in most pregnant rats of hypothyroid group comparing with control pregnant females.Present showed significant decreased at (P≤0.05) in mean length, weight and number of fetus at (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation in all embryos related to hypothyroid rats compared to control. Histological study with light microscopy on sections from thyroid gland related to rats with induced hypothyroid showed reduced, small follicles with empty or little colloid substance, each follicle lining simple cuboidal or flat epithelium, an increased with stroma, variable size of follicles most of them smaller compared to control thyroid gland from control rats, which showed normal lobules, large follicles filled with colloid, and poorly stroma.Sections from uterus, ovaries, oviduct related to hypothyroid rats showed variable histological changes ranged from mild to severe like abnormal reduced layers of uterus, irregular uteri folded, degenerated with simple epithelial tissue that lining the uterus villi, haemorrhage in uterine gland, deposition of collagenous fibers, infiltration of lymphocytes and accumulation of adipose tissue. Ovaries from hypothyroid rats showed variable changes with ovaries follicles maturation, accumulated of adipose tissue, decreased graffian follicles, infiltration of lymphocytes and deposition of collagenous fibres with degenerated of lining epithelial layer compared with control ovaries.Microscopic observations of placenta sections from hypothyroid rats at different period of gestation revealed loss tissue, reduced placental layers thickness, necrosis in the peripheral regions, degenerated cytotrophoblasts that surrounding capillaries, the trophoblast giant cells appeared with degenerative changes in labyrinth and spongiotrophoblasts regions. undifferentiated fetal blood capillaries compared to control which the sections have normal structure differentiated to decidual basalis and labyrinth zones, the junctional zone composed of outer giant cells that separated the decidua basalis and trophospongium.In this study the ultrastructural changes in thyroid gland from maternal and fetus related to hypothyroid rat at (14.5, 16.5, 18.5, 20.5, 21.5) days of gestation were identified by electron microscopy and included changes in apical cytoplasm of follicular cells to microvilli, blebs and protrusions also dilated rough endoplasmic reticulum, increased lysosomes, the empty vacuoles more than the dense vesicles, increased mitochondria and Golgi apparatus cisternae, changes chromatin and more cellular debris. Biochemical results in thyroid hormones revealed to significant increase in mean concentration of (TSH) in pregnant females with hypothyroidism during gestation periods in compared with control group, also there was an decrease in both (T3, T4) non significant, significant increased concentration in serum of pregnant rats on treated group with (Ptu) compared with their concentration of control pregnant rats.Levels of some oxidative enzymes (GSH & MDA) was estimated in this study and there was significant increased,non significant in their concentrations in serums of rats with induced hypothyroidism comparid to control group during each period of gestation (14.5, 16.5, 18.5, 20.5, 21.5) day. The study determined some of haematological parameters such as (R.B.Cs) count, (H.b) level and (P.C.V) ratio which showed non significant at (P≤0.05) hypothyroidism rats compared with control rats at the same period of gestation. Also the results revealed non significant with (T.L.C), (D.L.C) in all pregnant rats with hypothyroidism compared to control rats, the data showed an increased with lymphocytes and Neutrophils and Esoinphils cells in smears prepared from hypothyroidism rats compared to control group

عزل وتشخيص احد قلويدات الايض الثانوي لبعض الطحالب ودراسة فعاليته الحيوية == Isolation and identification one alkaloid of secondary metabolites from some algae and study of biological activity

Author name: انفال نوري عباس اللفتة
Supervisor name: احمد محسن عذبي | اقبال جاسم الاسدي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study includes an isolation, Identification and purification of three species of algae, two of them belonging to cyanobacteria they are Oscillatoria brevis and Nostoc carneum . The third one was Enteromorpha intestinalis which belong to green algae which was from different location in Basrah .There are two extract were prepared from the algal species Alcohol , and alkaloid extracts , Alcoholic extract prepared to know what was compound it had and alkaloid extracts to test bioactivity of algae Cytotoxcity also was carried out on human red blood cells, the results revealed that alkaloid extract from E.intestinalis was nontoxic, whereas bioactive compounds isolated from O.brevis , N.carneum. Showed hemolytic action .The bioactivity of alkaloid extract was examined to elucidate their on ability to inhibit the growth of gram Positive and negative bacteria. .Biological activity of alkaloid extracts of three algae isolated were determined by using the Minimal Inhibitory Concentration (MIC) againsttwo bacterial isolates. And also test the effect of filtrate pure algal culture on growth of plant pathogenic fungi ,also apparently that the algal alkaloid extract and isolated compounds exhibits antioxidant.The antitumor activity of the algal alkaloid extract from E.intestinalis against Rhabdo myosarcoma cell line was examined. The results showed that crude alkaloid extract possessed an antitumor bioactivity at low concentration 0.78mg / ml .In this study also was carried out to investigate the possibility of preventing the hypercholesterolemia by using alkaloid extract of E.intestinalis .it also aimed to study the effect of use this extract in reducing the Plasma Total Cholesterol (TC) , Triglyceride (TG), Low - Density Lipoprotein (LDL) , Very Low - Density Lipoprotein (VLDL) and no effect on High - Density Lipoprotein (HDL).The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR) and Gas Chromatography / Mass spectrum (GC - MS). The results of such analyses showed that alkaloid extract from E.intestinalisa has three compound these are Hexadecanamide , Methenamine and Benzenedicarboxylic acid, diisooctyl est and alkaloid extract from N.carneum showed that has three compound 1,2 - Benzenedicarboxylic acid diisooctyles,ا9 - Octadecenamide, (Z) - )ااااا and Didecyl phthalate .Hexadecanamide purification from alkaloid extract of E.intestinalis depended on physical and chemical properties The identification of the compound was made depending on the active groups test and spectroscopic analyses including : Infrared (IR); Gas Chromatography / Mass spectrum (GC - MS) and this component showed bioactivity on gram. Positive and negative bacteria and it has also antioxidant activity

استخدام عوامل استحثاث لانتاج مؤيضات ثانوية من بعض عزلات انواع الفطر واختبار فعاليتها الضد بكتيرية والخلايا السرطانية Penicillium والكولسترول == Use of inducing agents in secondary metabolites production from some Penicillium isolates and assay their activity against bacteria , cancer cells and cholesterol

Author name: امل صالح عبد الرضا
Supervisor name: توفيق محمد محسن
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was designed to investigate active compounds from different Penicillium isolates ,that isolated from different sources , using different media . Eleven isolates related to five species : P. chrysogenum, P.paxilli , P. citrinum, P. cosmopolitanum, P. digitatum were isolated. After primary screening was performed , the best isolates that have high antibacterial activity against gram positive and gram negative bacterial was selected. The selected isolates were cultured on fermentation media according to the suitable media for each isolate, and the active compounds were extracted using ethyl acetate as a solvent. P. cosmopolitanum and P. chrysogenum were cultured on penicillin production medium (PPM) , P. paxilli was cultured on Aspergillus complete medium (ACM) and P. digitatum and P. citrinum were cultured on solid state fermentation media consist of wheat bran . The efficiency of fungal crude extracts from each isolate against the reference strains S. aureus NCTC 6571 and E. coli ATCC 25922 and against Candida albicans (pathogenic and reference strain)was examined, and the Minimum inhibitory concentration (MIC) was determinated for each extracts.The inhibition zone of fungal extracts against E. coli and S. aureus ranged between (20 - 50) mm , and they had high activity against C. albicans ( reference and pathogenic isolate) ranged between 25 - 45 mm.Induction of secondary metabolite production by the selected fungi was carried out using biological factor (Streptomyces sp.) , chemical agent (colchicine) and physical agent by UV radiation. Apparently that the biological induction by Streptomyces isolate was the best compared with the other treatments.The results revealed that the two fungal extracts ALO1 and Apax exhibit a high antioxidant activity at low concentrations and volumes of the extract compared to ascorbic acid as control. ALO1 had radical scavenging activity (RSA%) reach 99.9% at volume 3μl , and Apax had RSA 91.6% at concentration 500μg/ml. Also, the fungal extract ,ALO1, showed a significant activity as anticancer agent against RD - cell line. The IC50 of ALO1 was 8.5μl after 24h and 23.76μl after 72h. The hypercholesterolemia ability of ALO1 in vivo and in vitro was assayed . In vivo experiment designed using female albino mice and divided into three groups each one contain 8 mice : group 1 treated with 10μl , group 2 treated with 20μl of ALO1 , and group 3 treated with normal saline as control group. The results showed that total cholesterol , triglycerides, LDL and VLDL were significantly reduced when compared with control group while the HDL level was increased in comparing with control group. The chemical analysis of the crude extracts showed that Apen2 contained 259 μg/g of penicillin G when analyzed with HPLC apparatus . Also the GC mass analysis of ALO1 showed that Docasadionic acid initialize area76.18% and Palmitic acid initialize area 15.09% from the total identified compounds.Whereas the GC mass analysis of Apax showed that Hexandioic acid initialize area40% and Stearolic acid initialize area 35.57% from the total identified compounds.In addition , it was detected the presence of penicillin biosynthetic gene cluster : pcbAB, pcbC and penDE in P. cosmopolitanum and P. chrysogenum.The result showed that gene pcbC and penDE were found in the genomic DNA but the gene pcbAB was not amplified by PCR.

دراسة تصنيفية وبيئية لحشرات نصفية الاجنحة المائية وشبة المائية Heteroptera : Hemiptera في محافظة البصرة == Taxonomical And Ecological Study of Aquatic And Semiaquatic Insects(Heteroptera : Hemiptera) In Basrah Provience

Author name: اسماء عبد الزهرة سبع العيداني
Supervisor name: ضياء خليف كريم
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
University: University Of Basrah - College Of Education For Pure Sciences
Language: Arabic
University location: Basrah
First pages:
Abstract: Present studies dealing with the taxonomical and ecological studies of the aquatic Hemiptera : Heteroptera in eight stations in Basrah provience ,which are : Al - Medina , Al - Qurna , Basrah center ,Shat Al - Arab , Al - Zubair , Abu Al - Khasib , Al - Faw and Sullein Marsh, collection was done twise monthly during the period from April 2012 to March 2013 .Results showed the presence of nine species belonging to aquatic Hemiptera , three belonging the group Gerromorpha and six the group Nepomorpha,as follows : Group : Gerromorpha(Popov,1971)Family : Gerridae ( Leach , 1815)1 - Aquarius nebularis(Schellenberg,1800)Family : Mesoveliidae(Douglast and Scott , 1867 )2 - Mesovelia vettigera( Horvath , 1895 ) ( apterous )M .vettigera( Horvath , 1895 ) ( macropterous )Family : Macroveliidae3 - Macrovelia hornii(Uhler , 1872 )Grouop : Nepomorpha(Popov,1968)Family : Nepidae ( Latreille , 1802 )4 - Nepa cinerea( Linnaeus , 1758 )Family : Corixidae ( Leach,1815 )Subfamily : Cymatianiae ( Walton , 1940 )5 - Cymatia bonsdorffii(Sahlberg , 1819 )Subfamily : Corixinae ( Leach , 1815 )6 - Sigara(Vermicorixa) latelaris ( Leach , 1815 )Family : Belostomatidae ( Leach , 1815 )7 - Lethocerus fakir(Mayr , 1852)Family : Notonectidae ( Latreille , 1802 )8 - Anisops sardeus(Herrich - Schaeffer , 1849)AbstractBFamily : Pleidae ( Fieber , 1851 )9 - Plea leachi(McGregor and Kirdaldy,1899)Among the collected species ,six were regarded as new records to the Iraqi fauna of this order,they were : A. nebularis,M. vettigera( macrpterous) , M . vettigera( apterous), Ma.hornii , N.cinerea , C.bonsdorffii andP.leachi.Study also deal with the measuring of some ecological aspects like air and water temperatures , salinity , pH and dissolved oxygen ,and their effected on the monthly distribution of the aquatic Hemiptera. Temperature was the most effective aspect in the monthly distribution of the insects ,as the lowest numbers were recorded in Summer months and the highest number were in Winter months , the ranges of the air temperatures were 9 - 43º C and water temperatures were 2 - 33º C .The highest concentrations of salinity was recorded in Al - Zubair , Abu Al - Khasib and Al - Faw reached to 35g/l in Al - Zubair in July , the pH ranges were the same in all stations and was alkalid , their ranges were 7.3 - 7.8 , about the dissolved oxygen it was low and same in all stations except in Sullein Marsh which reached to 11 mg/l in January .The concentration of some heavy metals in water also studied like Fe, Pb, Cu and Zn , the highest concentrations were to Fe and Zn in Basrah center and Al - Zubair, and the highest concentration for Fe in Al - Zubair reached to 6362.21μ/L in August, while highest concentration for Zn in Basrah center reached to 1365.92μ/L in July, while in the other stations the concentrations were lowest .The study also used the water boatmen Sigara latelaris(Leach,1817) as bioindicators of heavy metals pollution in the water of Basrah provience , and taken Sullein marsh as control , and measuring the concentrations of the heavy metals in insects tissues , water and sediments , the results showed that the insects collected from the water of the center of the city can accumulate Fe andAbstractCZn in their tissues and the highest concentrations reached to 3562.16 and 3778.12 μ /gm for Fe and Zn in July , while in the insects taken from Sullein marsh were 286.25 and 285.17 μ /gm .The concentrations of heavy metals in water and sediments of the city center were measured and the highest concentration was for Fe and Zn , and reached to 1569.91 and 1365.91 μ /L of the water of city center , while reached to 215.01 and 223.14 from the same metals in the water of Sullein marsh , the concentrations of the same metals were recorded in sediments of the city center and reached to 2865.16 and 1982.56 μ /gm for Fe and Zn , and the highest concentrations of the same metals in Sullein marsh were 418.57 and 393.74 μ /gm .

دراسة تاثيرالسمية النباتية والوراثية لتراكيز مختلفة من الاوكسينات ومضادات التلوث في استحثاث الكالس الاولي لنخيل صنف الحلاوي. (Phoenix dactylifera L.) التم == Assessment of Phytotoxicity and Genotoxicity of different Auxins concentrations and decontamination agents on initiation of date palm (Phoenix dactylifera L.) primary callus of Hillawii cv.

Author name: اسراء عبد الرزاق حميد السامر
Supervisor name: صبيح داود محمد العطبي | محمد حمزة عباس
General topic: Biology
Specific topic: Plant - Tissue
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted to evaluate the effect of different concentrations of the growth regulators 2, 4 - D; Dicamba and NAA on the induction of date palm (Phoenix dactylifera L.) primary callus Hillawii cv., by analyzing morphological, biochemical; anatomical and molecular responses. Three concentration of 2, 4 - D (10; 50 and 100 mg/L), Dicamba (1; 5 and 10 mg/L) and 30 mg/L of NAA were examined during the course of this study. in addition, several antimicrobial agents were selected to evaluate their morphological and biochemical impacts on the primary callus of date palm including the antibiotics (Gentamycin 50 mg/L and Chloramphenicol 50 and 100 mg/L), as well as, the fungicides (Switch 1 g/L and Beltanol 1 ml/L), After induction of the primary callus from each treatments, the following results were obtained : 1. The results showed that the treatments with 2, 4 - D at 10 mg/L and Dicamba at 1 and 5 mg/L did not stimulate any callus growth over the time of incubation on MS medium.2. It was evident that the treatments of different growth regulators have a significant effect on the initial period (day) for callus initiation, the shortest period have been obtained with the low concentration of 2, 4 - D (50 mg/L) and Dicamba (10 mg/L) which were 67.57 and 66.75 days, respectively, while the high concentration of 2, 4 - D (100 mg/L) led to increase this period significantly up to 120 days.3. Both fresh and dry weight of date palm primary callus decreased significantly at the treatment of high concentration of 2, 4 - D (100 mg/L) up to two folds compared to the treatment at low concentration of 2, 4 - D (50 mg/L), similar trend of results were found with browning percentage and intensity.4. Biochemical analysis of produced date palm primary callus revealed that the high concentration of 2, 4 - D (100 mg/L) led to a significant increase in the total content of carbohydrates; Proline, Peroxidase activity and Phenolic compounds which considered as a stress indicators, and another decrease of total soluble proteins and free amino acids was accompanied with this treatment compared to the same growth regulator at low concentration and Dicamba treatments.5. Anatomical study revealed that asignificant damage was caused by the high concentration of 2, 4 - D (100 mg/L) on date palm primary callus, this damage was evident from the abundant and distribution of tannins compounds across the primary callus tissues, thus, was accompanied with high percent of browning, compared with the treatments of 2, 4 - D at low concentration, Dicamba and NAA.6. The analysis of Protein profile revealed the similarity between the patterns of control treatment (juvenile leaf of Hillawii cv.) and 2, 4 - D at low concentration, as well as, Dicamba. Similarity indices results for protein profile of date palm primary callus proved the genetic similarity of 100% between control treatment and both 2, 4 - D (50 mg/L) and Dicamba ones, while, the lowest similarity percentage was observed with high concentration of 2, 4 - D (100 mg/L).7. The results of RAPD marker analysis of the extracted genomic DNA form date palm primary callus using four deca oligonucleotide primers showed the efficiency of three primers (OPAR3; OPAR8 and P.650) in their amplification of examined DNA,. RAPD profile revealed an identical matching between control treatment and 2, 4 - D at low concentration, followed by Dicamba and NAA auxins, while, the increasing in 2, 4 - D concentration up to 100 mg/L led to disappearance and the appearance of new DNA bands compared to control treatment, with a highest genetic distance among other treatments.Similarity coefficient analysis showed that the highest genetic similarities were found between control treatment and 2, 4 - D (50 mg/L) with similarity index of 96% , followed by Dicamba and NAA treatments with indices of 74 and 66%, respectively, the lowest similarity index was obtained between control and 2, 4 - D at high concentration which was 27%.8. The screening results of antimicrobial agents showed that the treatment with Switch fungicide (1 g/L) and Gentamycin antibiotic ( 50 mg/L) did not stimulate any callus growth over the time of incubation on MS medium supplemented with either 2, 4 - D or Dicamba.9. Beltanol fungicides (1 ml/L) treatment had a negative effect on the initiated primary callus either by 2, 4 - D or Dicamba, its treatment led to a high significant reduction in total solubleprotein and free amino acid content compared to control results, while an increase of total carbohydrates, Phenolic compounds, Proline and Peroxidase activity was detected with Beltanol fungicide.10. The Chloramphenicol treatment at low concentration (50 mg/L) showed positive results on the growth of primary callus at 2, 4 - D or Dicamba auxins according to morphological and biochemical analysis.

التحلل والمعالجة الاحيائية للمركبات الهيدروكاربونية النفطية بوساطة سلالات Streptomyces وNocardiopsis المعزولة من الترب الملوثة بالنفط في محافظة البصرة - العراق == Biodegradation and bioremediation of petroleum hydrocarbons by Streptomyces and Nocardiopsis strains isolated from oil contaminated soil of Basrah Governorate - Iraq

Author name: احمد عبد برغال الاسدي
Supervisor name: كوثر هواز مهدي | نادية عبد الامير المظفر
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
University: University Of Basrah - College Of Science - Department Of Biology
Language: Arabic
University location: Basrah
First pages:
Abstract: سبعة سلالات من البكتريا الخيطية تعود الى جنسي Streptomyces وNocardiopsis عزلت من ترب شركة مصافي الجنوب الملوثة بالنفط الخام في البصرة جنوب العراق، وقد تم اختبار قدرتها على النمو بوجود النفط الخام باستعمال وسط ملحي سائل مزود بالنفط الخام كمصدر وحيد للكربون. شملت السلالات على S. variabilis 7525 وS. variabilis SW75 وS. cellulosae XFB - T وS. parvus S86 وS. bacillaris S4BW2 وS. flavoviridis VITHM - 1 وN. synnemataformans ITD - 3 بنسبة تشابه من 99٪ الى 100٪. في هذه الدراسة تم العثور على سلالات جديدة من البكتريا الخيطية المعزولة من التربة الملوثة شخصت باستعمال تحديد تتابعات جين الحامض النووي الريبوزي 16S rRNA وقد اظهرت السلالات قدرات مختلفة على تحلل النفط الخام في الوسط الملحي السائل من 50.2٪ الى 72.4٪. كما تم تقييم تاثير الظروف الزرعية من درجة الحرارة والدالة الحامضية وتركيز النفط الخام والعناصر النزرة في نمو سلالات البكتريا الخيطية وتحلل النفط الخام، وقد لوحظ ان اعلى نمو للخلايا واعلى تحلل للنفط الخام كان 80٪ في ظروف زراعة مثالية ( درجة حرارة 30 ᵒم ودالة حامضية 7 بعد 10 ايام من الحضن وزادت هذه النسبة الى 85٪ من قبل السلالة S. cellulosae XFB - T بعد اضافة العناصر النزرة.في حين اظهرت نتائج اختبار التحلل الحيوي للنفط الخام في التربة من قبل سلالات البكتريا الخيطية منفردة بعد 60 يوما من الحضن تحت ظروف مختلفة ، اظهرت ان اعلى قيمة للتحلل كانت 87 ٪ من قبل النوع S. bacillaris عند درجة حرارة 30 ᵒم وحجم لقاح مضاف 10مل/ 25غم تربة ومستوى رطوبة 40٪.المعالجة الاحيائية هي ستراتيجية فعالة لتنظيف المواقع الملوثة بالهيدروكربونات. ونتيجة تاثر الكائنات الحية الدقيقة من تلك التربة يحدث لها اضطراب وبالتالي يتم انتقاء الاحياء المجهرية القادرة على استهلاك الهيدروكربونات لاستخدامها في هذه التقنية الفعالة في التعامل مع الملوثات الهيدروكربونية. الهيدروكربونات التي تتفاعل مع نسجة التربة والكائنات الحية الدقيقة هي التي تحدد مصير الملوثات وطبيعتها الكيميائية والقدرات الهادمة للميكروبات. الاثار المحتملة لنشارة الخشب، وخليط من روث الابقار والغنم وتعديل المكملات الغذائية لتحفيز الكائنات الدقيقة الاصلية وزيادة المعالجة البيولوجية للهيدروكربونات، هذه العملية تم اجراءها باستعمال طريقة المعالجة خارج الموقع بطريقتي الكومة والمفاعل الحيوي، بخلط التربة الملوثة مع نشارة الخشب والروث الحيواني ودعم التربة بالمغذيات والعناصر النزره مع توفير الرطوبة الملائمة والظروف المواتية لنمو الكائنات الحية الدقيقة كما قدمت التهوية الصناعية للتربة في الكومة والمفاعل بوساطة شبكة من الانابيب المثقبة لمحاكاة المعالجة الحيوية التي استمرت مدة 90 يوما.خلال تلك الفترة، رصدت الهيدروكربونات النفطية الكلية (TPHs) وازالة الالكانات الاعتيادية والتغيرات في المجتمعات البكتيرية. وقد اظهرت النتائج ان (TPHs) قد انخفضت من 52غم/كغم الى 10.6 غم / كغم بمعدل تحلل بلغ 79.6٪ ومعدل تحفيز كان 71.4٪ في تجربة الكومة ومن 52غم/كغم الى 13.5 غم / كغم بمعدل تحلل بلغ 74٪ ومعدل تحفيز كان 63.5٪ في تجربة المفاعل الحيوي. وخلال مدة 45 يوما من الاختبار بلغت اعداد الكائنات الحية الدقيقة من البكتيريا المضافة والمستوطنة التي تحلل المواد الهيدروكربونية اعلى مستوى لها 2 × 710 خلية/ غم و1.1 × 710 خلية/ غم في الكومة والمفاعل الحيوي على التوالي.بناء على هذه المعطيات، نستنتج ان المعالجة الحيوية خارج الموقع افضل ستراتيجيه غير مكلفة وفعالة وصديقة للبيئة وبالتالي قد توفر خيارا قابلا للتطبيق لمعالجة التربة من الملوثات الهيدروكربونات النفطية. | Seven actinomycetes strains from genera Streptomyces and Nocardiopsis isolated from hydrocarbon contaminated soil in Basra Governorate, Iraq were selected for their capacity to grow in the presence of crude oil. Their growth rates and biodegradation ability were investigated in mineral salt media supplemented with light Remella crude oil. These strains consist of Streptomyces variabilis 7525, Streptomyces variabilis 5W75, Streptomyces cellulosae XFB - T, Streptomyces parvus S86, Streptomyces bacillaris S4BW2, Streptomyces flavoviridis VITHM - 1 and Nocardiopsis synnemataformans ITD - 3, that the sequence identity range from 99% to 100%. Our study find a new actinomycetes strains isolated from contaminated soil. these strains appears different capacity on the degradation of crude oil in mineral salt media , the highest was found to vary from 50.2% to 72.4%.The effect of the cultivation factors (temperature, pH, and concentration of crude oil and trace elements ) on growth of the actinomycetes strains and crude oil degradation was evaluated. The highest cell growth and the amount of crude oil degraded 80% were observed in optimized cultivation conditions (30oC and initial pH 7) after 10 days, this ratio reached to 85% by addition of trace elements solution at same condition by S. cellulosae XFB - T.The biodegradation test lasted 60 days in soil by actinomycetes strains at different factors shown the highest of the crude oil was removed in experiment that inoculums with 10ml of each bacterial strain, value 87% by Streptomyces bacillaris S4BW2 at 30oC. Bioremediation is an efficient strategy for cleaning up sites contaminated by hydrocarbons. Microorganism of soil is affected by hydrocarbon disturbance thus selective enrichment of hydrocarbon utilizers occurs. The technology is very effective in dealing with petroleum hydrocarbon contamination. Hydrocarbons react with the soil matrix and microorganisms determining the fate of the contaminants relative to their chemical nature and microbial degradative capabilities. The potential effects of sawdust, and mixture of cow and sheep dung as amendment nutrient supplements to biostimulate autochthonous microflora and augmentation for hydrocarbon bioremediation were investigated in test biopile and bioreactor. The soil was ground and fluffed by admixture of 1.5% sawdust, then supplemented with the necessary minerals and watered to provide conditions favoring microorganism growth industrial aeration was provided in pile by system of a abundant perforated drainage - pip network to simulate bioremediation treatments through a 90 - day period. During this period, we monitored total petroleum hydrocarbons (TPHs) and n - alkanes degradation and changes in bacterial communities. The (TPHs) had been reduced from 52 to 10.6 g/kg (79.6 %), the rate of Biostimulants Efficiency (BE) was (71.4%) in biopile and from 52 to 13.5 g/kg (63.5 %) in bioreactor . In soil, the dominant microorganism population comprised Gram - positive bacteria from actinomycete group and autochthonous microorganisms which decompose hydrocarbons reached highest level 2 x 107 and to 1.1 x 107 CFU/g at 45 days in biopile and bioreactor respectively . Based on these data, the study conclude that is ex situ ( Biopile and bioreactor ) experiment the best strategy, inexpensive, efficient, and environmentally friendly and may thus offer a viable choice for petroleum hydrocarbons - contaminated soil remediation.

تمثلات الاخر في كتب الرحلة العربية رسالة ابن فضلان انموذجا == The other representations in the books of the Arab journey, Ibn Fadlan's l etter as a sample

Author name: منى حسن علي
Supervisor name: احمد حياوي السعد
General topic: Arabic Language and Literature
Specific topic: Language
Degree: Doctorate
University: University Of Basrah - College Of Literature - Department Of Arabic Language
Language: Arabic
University location: Basrah
First pages:
Abstract: After we stood on the text of Ibn Fadlan's journey, we presented intensive preoccupations about Ibn Fadlan's vision and perceptions of the other in his social, spatial and religious diversity, which allowed us to monitor and follow the behavior of the other, so the study opened on the nature of the other social and his life system of customs and traditions, Food, clothing and others .Since the journey is a travel from one place to another over time, we had to stand on the other spatial and the nature of the geographical environment of different climate, water, land and natural phenomena, as well as the implications carried by some places across the spaces of the journey and spatial extensions and their association with time..The religious' other takes share of the lesson , so we show theIbn Fadlan's view to the religious beliefs and differences , and thenature of the dialogue with the other, the religious centrality as wellas the concept of identity and concepts related to building the cultural identity of the individual and society.According to all these data, we find that the cultural approach is best suited to reading this heritage text according to the concepts of assimilation and the other, which are one of the most important concepts of cultural criticism applications and because this critiqueprocedure - that across surround the text to deals with the activity of human - detection the productions processes of cultural in general and approach to the cognitive subjects and the cultural reality that contributed to its crystallization.We sought to combinethe analysis and draw conclusions and reached conclusions that can be summed up in the following points : • It was estimated to Ibn Fadlan that , he was the first eyewitness in the fourth century AH to describe the country of the Russians and some of the areas of Eastern Europe, which no one arrived before him on his trip which is regarded exceptional and unique, varied interests between the geographical description and historical, cultural and civilization.• The journey is rich in knowledge accumulated diverse and holds in it many of the people's patterns of cultures which intended by travelers in their different cultural and civilizational environments and it is full of content of the experiences and experiences that benefit human sciences in various genres.• We observed in the journey many pictures of the other depending on the position taken by others, it shows us that Ibn Fadlan's view of the other in his social environment is not typical or fixed at all, but ranged from rejection and acceptance , as he censure some of the habits that do not match with what is in his country values, in oher hand we find him l applaud Some of them were to their similarity with their style. Thus, it was clear to us that his cultural references and cultural composition influenced his receiving andrepresenting the other. • Ibn Fadlan was objective in describing people with whom he differed in civilization, religion and tradition. He sometimes avoided commenting or declaring his personal opinion directly, but he tended to summarize and abbreviate, and sometimes his descriptive language was mixed with surprise at times and denouncing another of the unusual practices in his culture and customs Which he sometimes tried to change.• Although the journey was purely religiously declared as an official mission to non - Muslim countries to teach religious law, we found that it was not devoid of political and exploratory dimensions, nor was it free of the curiosity of the traveler based on the scientific and cognitive survey. As well as it benefit in aspects of it geography and history, especially when it mentions the distances, dimensions and days separating the countries that have penetrated them.• The place formed variant other and not familiar space, it was sometimes harsh ,brutal and hostile, especially with regard to nature and variability, and did not neglect the places news miraculous, which was popular within the public taste and was able to find a presence within the official texts represented by the travel text.• The religion formed a fundamental criterion and an effective tool in Ibn Fadlan's view of his rule over the other, which is represented in , the rejection and acceptance that is because of the connection of the close traveler to his reference, which is basically an integrated Islamic system of cultural, religious and intellectual values, especially in the places that requires self - comparison with the other in some way It can be seen from a hidden feeling of cultural superiority based on his belief in his cultural references that see non - Arab and non - Muslim peoples are barbaric and uncivilized.• The traveler not only writes his observation and monitoring then recording the data he encounters, but allowed himself to highlight his religious identity and victory in front of the identity of the other without domination or robbery, passingthe civilized context, loaded with the collision that characterized the relationship of a Muslim with an infidel when he crosses his space , we find him sometimes takes a neutral stance through expressing his opinion, and at other times expresses expressions of surprise, and ridicule.• Ibn Fadlan deal with the other depending on the difference and rejection on the one hand, acceptance, compatibility and neutrality on the other hand with the same psychological harmony, whether penetrating the spaces of Islam or disbelief, we note that his relationship with the other is a positive relationship and non - hostile and far from superiority, based on recognition of cultural differences Among peoples. • Finally, it can be said that the resulting images and representations of the other were governed by Ibn Fadlane's religious repertoire, which serves as a reference and a measure to consider the superiority of Islamic religious, cultural and political centrality of the Abbasid Caliphate then.

المفاجاة الاسلوبية في الخطاب القراني == STYLISTIC SURPRISE IN THE QURANIC SPEECH A PRACTICAL STYLISTICS

Author name: منـذر زيارة قاســم
Supervisor name: ازهار علي ياسين
General topic: Arabic Language and Literature
Specific topic: Language
Degree: Doctorate
University: University Of Basrah - College Of Literature - Department Of Arabic Language
Language: Arabic
University location: Basrah
First pages:
Abstract: تعد دراسة المفاجاة الاسلوبية دراسة للابعاد التاثيرية للخطاب القراني لدى المتلقي , وهي ضمن اطار الدراسات اللسانية الاسلوبية المعاصرة , وبذا تكون محاولة للخروج من الاطر البلاغية والتفسيرية لقراءة النصوص الابداعية ومنها القران الكريم والمفاجاة الاسلوبية اصطلاح ظهر في اوربا اواسط القرن العشرين على يد الروسي رومان جاكبسون , وعرفها " تولد اللامنتظر من خلال المنتظر " , وطورها الامريكي ميخائيل ريفاتير , وجعل قيمة كل ظاهرة اسلوبية تتناسب تناسبا طرديا مع حدة المفاجاة وتتناسب تناسبا عكسيا مع تكرراها وتواردها , وتتلخص بادخال العناصر غير المتوقعة مع العناصر المتوقعة في سياق النص . وتعمل المفاجاة في ضوء معايير واضحة وهي الاسلوب , والسياق , والقارئ , والتلقي . التي تعد مواطن تعيينها . ويعد الخطاب القراني بوصفه اثرى النصوص الابداعية في التراث العربي بيئة خصبة لدراسة المفاجاة الاسلوبية في ضوء دراسة وسائل التاثير في المتلقي لذلك الخطاب الالهي والاثار الجمالية الناتجة من ذلك فجاء البحث في تمهيد بين المفاجاة والخطاب في اللغة والاصطلاح . وتناول الفصل الاول منه المفاجاة الاسلوبية في ضوء نظريات التواصل اللساني والوظائف اللغوية والتحليل الوظائفي لها . وجاء الفصل الثاني لدراسة الاثار الجمالية للمفاجاة في ضوء مستويات الخطاب القراني الشكلية وتمحور الفصل الثالث لدراسة تلك الاثار على المستوى الدلالي متخذا من المفارقة القرانية انموذجا للدراسة .وختمت البحث بعد حمد الله سبحانه بالتركيز على اظهار وسائل الاثارة والتاثير والقناع والامتاع في الخطاب القراني التي اسهمت في فهم الخطاب القراني في ضوء المفاجاة الاسلوبية | The study of the stylistic surprise is deemed a study of the influential dimensions of the Quranic speech of the recipient. It is within the framework of the contemporary linguistic, stylistic studies, and hence there's an attempt to get out of the rhetorical and interpretive frameworks to read creative texts including Quran. The stylistic surprise appeared in Europe in mid - 20th century by the Russian, Roman Jacobson, who defined it as "the origination of the unforeseen through the foreseen". And this concept was developed by the American, Michael Revatier, who made the value of each phenomenon stylistically direct to the severity of the surprise, and inverse to the recurrence. This could be summed up through bringing together unexpected elements and expected ones in the context. The surprise operates in light of clear criteria specifically style, context, reader and recipient. The Quranic speech, as the most creative text, has enriched the Arab heritage in a fertile environment in order to study the stylistic surprise by studying the means that influence the recipient of that divine speech, and aesthetic effects resulting from it. This study consists of an introduction on both a surprise and speech in language and terminology. The first chapter has made a reference to the stylistic surprise in the light of theories of linguistic communication, linguistic functions and functional analysis. The second chapter is devoted to studying the aesthetic effects of the surprise in light of the levels of the structural Quranic speech. While the third chapter has focused on studying these effects at the semantic level, taking the Quranic paradox as model of the study. The study has closed up with a focus on disclosing the means of excitement, persuasion influence and joy available at in the Quranic speech, which contributed to the understanding of the Quranic speech in the light of a stylistic surpris

تمثلات الهوية في تراث الجاحظ : دراسة في ضوء النقد الثقافي == Identity Exem plifications in Al - Jahiz,s Heritage : a study from a cultural criticism perspective

Author name: مريم عبد النبي عبد المجيد
Supervisor name: احمد حياوي السعد
General topic: Arabic Language and Literature
Specific topic: Language
Degree: Doctorate
University: University Of Basrah - College Of Literature - Department Of Arabic Language
Language: Arabic
University location: Basrah
First pages:
Abstract: Identity represents a feature or certain features of another corresponding entity or entities. In fact, identity is a controversy among many philosophers, scholars and creativists. Thus, it represents the individualistic and collective belonging, and its configurations behave according to the nature of this belonging and the incurred changes. This study examines the potential values in categorizing the identities in the traditional Arabic heritage as exemplified by Al - Jahiz discourse in light of the cultural criticism approach and its connection with the cultural patterns of the Arabic society. It also studies the realizations, manifestations and the old roots of identity which dates back to remote times and deeply - rooted within other peoples, civilizations and religions.There is a detection of its danger in transmitting the meanings of ignorance, exile, exclusion, booing and atonement on the part of the other who is ethno - and religion - deviant. These meanings have their consequences which feed the seeds of arrogance, enmity, hatred, conflict and segregating the Ego from the Other. In addition to these, there is the breaking of the bonds of peace and love with planting the seeds of rupture and distortion in society and among nations and religions. Al - Jahiz,s discourse is associated with categorizing identities and its manifestations in relation to the Ego and the Other, or ,We, and ,They,. Identity was a basic concept in his writings, and he has a wide readership and his works were highly culturally consumed from past time till nowadays. Since the appearance of printing, his works have still been reproduced in elegant copies celebrating it as a typical example of literature and a first - degree pedagogic, cultural material, which is rarely absent from the curricula from the Gulf to the Ocean. This study is of three chapters with a prelude on (Al - Jahiz,s Discourse and its Exemplifications of Identity).The basic dimensions constituting Al - Jahiz,s discourse of exemplifying identity with its implied patterns that are also associated with the cultural Arabic realizations have been discussed. The first chapter also included studying the moral and the physical identity seen as the basis of recognizing the Self and the Other.The researcher has accounted for Al - Jahiz,s exemplifications and views ofmen and women identities and those of biological disabilities. In the second chapter, the religious identity has been studied. In its first section, the realizations of Muslim identities have been examined together with its phenomena and consequences uncovering a serious side of the Mu,tazila thought and their views of the Other having different dogmas. In the second section, the researcher the identity of the Jews, Christians and the Magi as manifested in Al - Jahiz,s perspective of the different - religion Other, together with what is implied in this perspective of meanings such as exile, exclusion, the moral and physical mocking. The third chapter examines the ,international identity, where the researcher studies the pstterning values in Al - Jahiz,s discourse reflecting subliming, favouring and devaluing in the context of identifying the identity of the Arabs, Persians, the Turks, the Sudanese from both positive and negative sides. After that, there is a conclusion chapter with summarizing the most important results arrived at. Then, references finalize this study.

الابعاد التداولية في شروح نهج البلاغة == Pragmatics in the Interpretations of Nahj Al - Balagha (Rhetoric Approach)

Author name: محمد مهدي حسين
Supervisor name: عبد الحسين علك المبارك
General topic: Arabic Language and Literature
Specific topic: Language
Degree: Doctorate
University: University Of Basrah - College Of Literature - Department Of Arabic Language
Language: Arabic
University location: Basrah
First pages:
Abstract: Al - Balagha has been of great importance in the Islamic and humanitarian system since it was written by Al - Sharif Al - Ridia (rh) to the present day. This is due to its preoccupation with various aspects of life on the one hand, and for its author's great reputation from the other hand. Studies and research about this book has been still working. Many of the different achievements in the knowledge sectors come at the forefront of this approach (the interpretations of Nahj Al - Balagha) have represented a great cognitive treasure in various fields and research; linguistic, rhetorical, religious, and historical. Time has kept a number of those interpretations. In spite of the large number of studies and research that have been developed in the interpretations of the approach, most of them have been in the fields of (grammar, rhetoric, or signifier), as well as many of them limited to a specific explanation. The researcher has not noticed a study aims to interpret contemporary pragmatics; therefore, he has decided to write about (Pragmatics in the interpretations of Nahj Al - Balagha). The study concentrates on applying contemporary pragmatics automatically to the code of annotations, and to interpret what the scholars presented through their analytical model of the deliberative nature of the upper discourse. Due to many studies have been made in pragmatics, the study has left it to avoid repetition. Consequently, Introduction has focused on ( Changes in the Pragmatic approach in the Interpretaions of Al - Balagha). It discusses two significant points : text writing (Nahj Al - Balagha) and the chronological divergence between Al - Nahj and interpretations with reference to the difference in reading Al - Nahj by interpreters based on the deliberative approximations they adopted. The first chapter of the study is concerned with the descriptive dimension in the interpretations of Nahj Al - Balagha. It is divided into two sections. The first one deals with the interpretive methods and the interpretative practice based on the interpretive methods of the narrators (deletion, submission, delay, and referral). The second one (the intention between the statement and the hint) explains how interpreters addressed the purposes of discourses of the upper discourse based on the graphical aspect of eloquence (metaphor, simile, imagery, and symbolism). The second chapter explores the communicative dimension in the interpretations of Nahj Al - Balagha which is also divided into two sections. In the first section (communication variables and competencies), the researcher has presented the variables of the communication process between the discourse of the approach and the participants through the Jacobson plan, as well as as the deliberative competencies of the participants (linguistics and encyclopedias). While the second section focuses on (communication strategies) in the interpretations. It studied three strategies (implicit, guiding, and solidarity). The third chapter is devoted to analyzing the evidence dimension in the interpretations of Nahj Al - Balagha. It falls into two sections. Section one (the evidence indicators) demonstrates the issueof the imamate by the interpreters through the discourse of approach and their emphasis on the most important mechanisms of this evidence ((kinship, allegiance, text, knowledge, and courage). As for section two, it analyzes evidence and mechanisms of opposition dialogue) which is the debate and the evidence and the narration of the evidence. The thesis ends with conclusion sums up the findings of this study
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