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الصورة البكتيرية والنسيجية المرافقة لالتهاب اللوزتين المزمن والادينويد عند الاطفال == Bacteriological And Histopathological Profile Associated With Chronic Tonsillitis And Adenoid Hypertrophy In Children

Author name: رشا محسن كاظم الحسيني

Supervisor name: حبيب صاحب نهر | صفاء حسين الطريحي

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Babylon - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Babylon

First pages:

Abstract: This study aimed to investigate the bacterial profile of surface and core of infected tonsils and adenoid tissues and histopathological examination of these organs.A total of 122 samples represented by 63 excised tonsils and 59 excised adenoids in addition to 57 blood samples were collected from 70 patients who were referred to Hilla Teaching Hospital (Ear, Nose and Throat unit) in Hilla city within a period of four months from November 2015 to February 2016. Those patients weresuffering from chronic tonsillitis and adenoid hypertrophy. The ages of those patients ranged from 2 to 15 years, 41 (59%) of them were males and 29 (41%) were females.The study included three main parts. The first one was the bacterial diagnosis based on relied diagnostic procedures. Second part was the detection of antistreptolysin O (ASO) by using latex agglutination test, while the third part included histopathological study to detect the pathological lesions that occur during these infections.The results indicated that, the most age group being susceptible for chronic tonsillitis and adenoid hypertrophy was the group of 7 - 10 years, represented 33 patients with a percentage of (47%). Among this group, males were more susceptible with percentage of 20 (29%) compared to female patients13 (19%).The surface of all samples (tonsil and adenoid) revealed positive results for bacterial culture, while in the core of tonsil only 44 samples (70%) were positive for bacterial culture while 19 samples (30%) were negative results (no growth). As in tonsils, the core of adenoid revealed positive results in 32 (54%) samples, while 27 (46%) samples were negative results.The suspected isolates were fully identified by using bacteriological, biochemical and Vitek 2 techniques (for two isolates because of those isolates give variable biochemical test results which confused their diagnosis that lead to using of vitek system technique to diagnosis them, these isolates were rare bacteria that infect tonsil and adenoid). The most common types of bacterial isolates were Staphylococcus aureus which accounted for 75 isolates (23.6%), followed by Streptococcus pyogenes 61isolates (19%), Escherichia coli 57 isolates (18%), Klebsiellae pneumoniae 55 isolates (17.3%), Hemophilus influenza 37 isolates (11.6%), Sphingomonas paucimobilis 14 isolates (4.4%), Staphylococcus epidermides 7 isolates (2.2%), Pseudomonas aeruginosae 6 isolates (2%), Pseudomonas stutzeri 5 isolates (1.6%), and Staphylococcus saprophyticus only one isolate (0.3%).Regarding the bacterial population, there is a difference between tonsils / adenoid surface and tonsil/ adenoid core culture were the most predominant isolate from tonsil surface was Staphylococcus aureus and K. pneumoniae from tonsillar core, while from adenoid surface was Streptococcus pyogenes whereas E. coli from adenoid core.The ASO titer in patients with or without group A streptococcal infections were determined by using latex agglutination test. The results revealed that 8 samples (14%) exhibited positive results, while 49 samples (86%) exhibited negative results out of 57 samples. The results showed statistically no significant differences between patient age groups but showed highly significant differences between males and females.Eight specimens were taking for histopathological examination randomly, four samples were adenoids and four samples were tonsils. The histopathology examination of tonsil and adenoid showed the presence of lymphoid hyperplasia,multiple lymphoid follicles, vascular congestion, fibrosis, germinal centers, infiltration of inflammatory cells, necrosis and apoptosis of tissues

التشخيص الجزيئي لتجرثم الدم لدى الاطفال حديثي الولادة بواسطة استخدام تفاعلات البلمرة المتسلسل في مدينة الحلة == Molecular Detection of Neonatal Sepsis By Using PCR In Hilla City

Author name: نور حامد عباس المرزوك

Supervisor name: الهام عباس بنيان الساعدي | ميساء صالح الشكري

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Babylon - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Babylon

First pages:

Abstract: في هذه الدراسة, تم جمع 85 عينة دم من 85 مريض مصابين بتسمم الدم (حسب التشخيص السريري الاولي للطبيب), تتراوح اعمارهم بين 1 - 28 يوم من كلا الجنسين (الذكور 53 والاناث 32) للفترة من تشرين الثاني 2014 الى اذار 2015.توضح نتائج هذه الدراسة ان (52,9%) طفلا حديث | Neonatal sepsis can be defined as any systemic bacterial infection confirmed by a positive blood culture in the first month of life. In this study, (85) blood specimens were collected from (85) patients with suspected septicemia, age ranged between 1to 28 days from both sexes (53 males and 32 females) during the period from November 2014 to March 2015.The results of this study revealed that (52.9%) are presented with late onset sepsis (infected during 7 - 28 days of their life), while the rest (47.1%) of the neonates are presented with early onset sepsis (infected during the first seven days of their life). The collected samples were investigated for bacterial isolation. Bacterial culture was positive in (7.06%) patients versus (92.9%) patients revealed a negative bacterial culture. The most common types of bacteria isolated were three isolates of Klebsiella spp. (50.0%), followed by one isolate Staphylococcus aureus (16.67%), one isolate E. coli (16.67%), and one isolate Enterobacter spp. (16.67%).The virulence factors of some bacterial isolates in this study were studied. The capsule was present in all bacteria isolated in this study (100%) except for Enterobacter spp. which did not possess the capsule.Colonization factor antigens were detected in all isolates. It was found that only Klebsiella spp. and E. coli (100%) have the ability to produce CFAI and CFAIII. Whereas Enterobacter spp. and Staphylococcus aureus (100%) have the ability to produce colonization factor antigen I only. The results also showed that all isolates of Klebsiella spp. and Enterobacter spp. did not produce hemolysin, while all isolates of Staphylococcus aureus and E. coli were able to produce hemolysin.Extracellular protease was also investigated. It was found that (100%) of all bacteria isolated in this study were able to produce extracellular protease.Biofilm formation was investigated in all bacterial species, and the results showed that all isolates of Gram negative bacteria and Gram positive bacteria form a biofilm.The ability of some bacteria to produce bacteriocin was tested and the results showed that E. coli and Klebsiella spp. were able to produce bacteriocin.In - vitro, the susceptibility of bacteria towards some antibiotics included in this study revealed that the amikacin and kanamycin are the most effective antibiotics to different types of pathogenic bacteria. Finally, molecular detection for bacterial sepsis was done by using specific primers (16 sRNA, rpoB and its). It was found that 16 sRNA genes were present in (20%) of samples and rpoB gene present in (18.8%). While its gene used for detection of fungi revealed negative results in all samples.

عزل بكتريا Bacillus cereus من بعض عينات الغذاء == Isolation of Bacillus Cereus From Some Foods Samples

Author name: ضحى عبد الله كاظم الظويهري

Supervisor name: حبيب صاحب نهر

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Babylon - Faculty Of Medicine - Department Of Microbiology

Language: English

University location: Babylon

First pages:

Abstract: كائن Bacillus cereus موجود في كل مكان وهوممرض منقول بالاغذية والذي يمكن ان يسبب نوعين من امراض الجهاز الهضمي : القيء والاسهال. في هذه الدراسه, تم جمع 140 عينه غذائية شملت (الرز, اغذية الاطفال، الدقيق، منتجات الالبان، اللحوم، الحليب , التوابل) جمعت عشوائيا | Bacillus cereus is a ubiquitous organism and a potential foodborne pathogen that can cause two types of gastrointestinal diseases : emesis and diarrhea. In this study, a total of 140 food samples included (rice, infant food, flour, dairy products, meat, milk powder and spices) were collected randomly from restaurants, food stores and various sale points in the local markets of the Hilla province during the period of November 2014 to April 2015 in attempt to isolate of Bacillus cereus from these samples and detection the prevalence of diarrheal (hbl and nhe) and emetic (ces) enterotoxins genes by using Polymerase Chain Reaction (PCR). All bacterial isolates were identified by cultural, microscopic and biochemical examinations. The results showed that 56 (40%) of samples showed positive growth for Bacillus cereus. The antibiotic sensitivity test of some isolates to 10 antibiotics was studied. It has been found that all B. cereus isolates were resistant to Ampicillin, Ampiclox, Cefotaxime, and Cephalexin, while all isolates were sensitive to Gentamycin, Ciprofloxacine and Chloramphenicol, but most of them showed different rate of sensitivity to one or more of antibiotics such as Neomycin 90% and 80% for both Tetracycline and Erythromycin. Polymerase Chain Reaction technique (PCR) was used to detect the ability of bacterial isolates to encode two types of enterotoxin genes, diarrheal and emetic genes. The results showed that 4(7.14 %) of isolates harboured hblA gene, 16 (28.57%) hblC, 5(8.92 %) hblD, 46(82.14%) nheA, 24(42.85%) nhe B, 26 (46.42%) nheC and 3(5.35%) of the isolates harboured Ces gene. On the other hand, through using PCR it was found that nhe gene was present in most isolates, while hbl gene was variable among the studied isolates. Moreover, the Ces gene was found only in isolates being isolated from rice samples, while it was not found in other types of samples.

تاثير مستخلص الكركم الخام على التعبير الجيني لمورثة البروتين المنظم للغشاء الحيوي لجرثومة Streptococcus mutans المعزولة من التجويف الفمي == Effect of Curcumine Crude Extract On The Gene Expression of Biofilm Regulatory Protein A (Brp A) In Streptococcus Mutans Isolated From Oral Cavity

Author name: نزار احمد شهاب

Supervisor name: عدنان حمد عبيد الحمداني | عدنان حمد عبيدالحمداني

General topic: Medicine

Specific topic: Microbiology

Degree: Master

University: University of Al-Qadisiyah - Faculty Of Medicine

Language: English

University location: Qadisiyah

First pages:

Abstract: جمعت 100 مسحة فموية من مرضى تسوس الاسنان وامراض ما حول الاسنان الذين تراوحت اعمارهم ما بين 15 - 65 سنة ومن كلا الجنسين والذين راجعوا المركز التخصصي لطب الاسنان في الديوانية للمدة من كانون الاول 2012 الى اذار 2013 لغرض قياس التعبير الجيني لمورثة البروتين ا | A total of 100 oral swabs were collected from patients suffering from dental caries and priodontal infections, ranging from 15 - 65 years old of both genders whom admitted to the Al - Diwaniyah Teaching Special Center of Dentistry within the period from December 2012 to March 2013, in order to quantify the gene expression of biofim regulatory protein (Brp A) in Streptococcus mutans isolates by using the Real Time polymerase chain reaction. According to morphological, cultural characteristics and biochemical tests by using VITEK - 2 system, 34/84(40.47%) isolates of S.mutans were isolated and identified among streptococcus species and the isolation percentages were 26/50(52%) in males and in females were 24/50(48%), while the age group (15 - 25 years old) was the most affected than the other groups. The statistical analysis showed no significant differences among the interaction between gender and age groups. Molecular detection of 16s rRNA gene was amplified to confirm the diagnosis of S.mutans isolates, where all isolates gave an amplicon size (151) bp, in addition to, the DNA sequencing was done in order to identify the nitrogen bases sequences and phylogeny of local isolates in comparison with the global isolates (Spain, Japan, Korea, USA, Canada, India), results revealed that the local isolates of S.mutans had a similarity percent (90%) with Spain isolates.Furthermore, the susceptibility of isolates to a variety of antibiotics have been investigated. It has been found that isolates had multiresistance to erythromycin(88.2%),ampicillin(76.4%),amoxiclave(70.5%),tetracycline(61.7%), nalidixic acid and amoxicillin (58.8%), chloramphenicol (55.8%). The statistical analysis showed a significant differences among tested antibiotics. Biofilm formation was investigated phenotypically by using tissue culture plate with crystal violet staining for resistant S.mutans isolates, the results revealed that all isolates were high biofilm former, furthermore, the effect of ethanolic extraction (50%) of curcumine in growth inhibition and biofilm reduction of S. mutans was assayed. The results showed an effect of curcumine in comparison with chlorhexidine(2%) as a control.The gene expression of biofilm regulatory protein A(Brp A) was quantified in comparison with housekeeping gene 16s rRNA as a reference gene of biofilm S.mutans by using reverse transcriptase polymerase chain reaction (RT - PCR). The results revealed that the expression (relative&absolute) of Brp A gene is decreased in the test treatment (S.mutans with 1% glucose+ 50% curcumine) in comparison with control (S.mutans grown in 1% glucose only).In conclusion, the present study established the role of Brp A gene in biofilm formation

دراسة تاثير استهلاك بكتريا Lactobacillus spp كمعزز حياتي على معدل كتلة الجسم ومحيط الخصر لدى عينة من مرضى السمنة في العراق == Study On The Effect Of Consuming Lactobacillus Spp. As Probiotic On The Body Mass Index (BMI) And Waist Circumference Among Sample Of Iraqi Obese Population

Author name: فخري سليمان العجيلي

Supervisor name: مى طالب فليح | لجين انور الخزرجى

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

العلاقة بين ظاهرة العج والغشاء الحياتي في المتقلبات المعزولة من مرضى خمج المجاري البولية == Relationship between Swarming and Biofilm in Proteus spp. isolated from Urinary tract infection patients.

Author name: ساره صلاح قدوري حسين

Supervisor name: بهاء عبد الله لفته الربيعي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

الاوكسجين الحرة المستحثة من العائلة المعوية تسبب الدنا في الخلايا الطلائية البولية البشرية == Reactive Oxygen Species Induced By Enterobacteriaceae Caused DNA Fragmentation In Human Uroepithelial Cells

Author name: اسامة باسم عبد الخالق الصفار

Supervisor name: هيفاء هادي حساني

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

انتاج وتوصيف المستحلبات الحياتية من بكتريا Serratia marcescens S10 المعزولة محليا == Production And Characterization Of Bioemulsifier From Locally Isolated Serratia Marcescens S10

Author name: انتصار فرعون احمد الملا

Supervisor name: شذى سلمان حسن

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

دور الدنا البكتيري في الموت الخلوي للعدلات == Role Of Bacterial DNA On Cell Death In Neutrophils

Author name: نوافل سليم خلف الالوسي

Supervisor name: هناء سليم یوسف | حارث جبار فهد المذخوري

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

عزل وتنقية وربط اضداد عديدة النسلية نوع Y من الدجاج الممنع ضد المشتضدات السطحية لفايروس التهاب الكبد الوبائي نوع B == Isolation ,Purification And Conjugation Of Polyclonal Antibodies Type Y From Immunized Hens Against Hepatitis B Surface Antigen

Author name: نجاة مؤيد نافع

Supervisor name: رغد السهيل | مجيد ارشيد سباح

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

استخلاص وتنقية وتوصيف الكليبوسين من بكتريا Klebsiella spp المعزولة سريريا == Extraction, Purification And Characterization Of The Klebovin From Clinically Isolated Klebsiella Spp.

Author name: لينا ارجمند عمر زاهد

Supervisor name: رشيد محجوب مصلح

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

تشخيص وتحري عن المقاومه الدوائيه MDR لبكتريا السلMycobacterium tuberculosis باستخدام طرائق مختلفه == A Study On Diagnosis & Multidrugresistance (MDR) Of Mycobacterium Tuberculosis Using Different Methods

Author name: هند حامد شاكر

Supervisor name: ضحى سعد صالح

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

دراسة تشخيصية لمرض تدرن سائل الجنب في العراق بطرائق تشخيصية مختلفة == A Diagnostic Study On Tuberculosis Pleurisy In Iraq By Different Diagnostic Methods

Author name: لارا غالي عبد علي العميري

Supervisor name: ضحى سعد صالح | احمد اسمر منخي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages:

عزل وتشخيص البكتريا المختزلة للكبريت في حالات تسوس الاسنان == Isolation and Identification Sulfate Reducing Bacteria in cases Dental caries

Author name: لقاء محمد خضير

Supervisor name: عدنان نعمة عبد الرضا العزاوي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Diyala - College Of Education For Pure Sciences - Department Of Biology

Language: Arabic

University location: Diyala

First pages:

الخصائص الفسيولوجية والجزيئية لــ Candida glabrata وCandida parapsilosis == Physiological And Molecular Characteristics Of Candida Glabrata And Candida Parapsilosis

Author name: ايناس كاظم عبد الامير الخزرجي

Supervisor name: زيدان خليف عمران المعموري

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science For Girls - Department Of Biology

Language: English

University location: Babylon

First pages:

فعالية الطبقة السطحية كممنع ومساعد مناعي والمستخلصة من بكتريا Aeromonas hydrophila المعزولة من مرضى الاسهال في محافظة بابل == Immunogenic And Immunoadjuvant Activities For Surface Layer Extracted From Aeromonas Hydrophila Isolated From Patients With Diarrhea In Babylon Province

Author name: لبنى عادل جميل النوري

Supervisor name: فريال جميل عبد | وجدان رضا تاج الدين

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science - Department Of Biology

Language: English

University location: Babylon

First pages:

الاستهداف الجزيئي لفايروس جون كنينكهام P16 Proteins ،B CellLymphoma Proteins لمرى ورم القولون == Molecular Targeting Of John Cunningham Virus (JCV),P16 Proteins And Bcl -2 Proteins In Colorectal Tumors Tissues

Author name: زهراء علي عبد الله

Supervisor name: شاكر حماد محمد العلواني

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science - Department Of Biology

Language: English

University location: Babylon

First pages:

التشخيص الجزيئي لبعض الخمائر المعزولة من بعض منتجات الالبان باعتماد تقنية البلمرة وتحليل تتابع الدنا == Molecular identification for some yeasts isolated from some dairy products based on PCR and DNA sequencing analysis

Author name: اية كريم جبار

Supervisor name: زيدان خليف عمران المعموري

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science For Girls - Department Of Biology

Language: Arabic

University location: Babylon

First pages:

الخلايا التنظيمية T ، والعلاقة البكتيرية والوراثية مع امراض صمامات القلب == T- Regulatory Cells, Genetic And Bacterial Relationship With Valvular Heart Disease

Author name: سيناء جابر علوان الحسيني

Supervisor name: عبد النبي جويد المعموري

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science For Girls - Department Of Biology

Language: English

University location: Babylon

First pages:

المعالجة الفطرية لمياه فضلات معمل دباغة الجلود في النهروان - العراق == Fungal treatment for wastewater leather tanning in Nahrwan –Iraq Introduction to message Board of the Faculty of Science for Girls - University of Babylon

Author name: ايناس رحيم مكطوف الكدساوي

Supervisor name: نداء شهاب حمد

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science For Girls - Department Of Biology

Language: Arabic

University location: Babylon

First pages:

التقييم المتوقع للعوامل الاحيائية الدقيقة والفسلجية لتطور التدرن البشري في محافظة ذي قار == Prospective Evaluation Of Microbiological And Physiological Factors Of Human Tuberculosis In Thi-Qar Province/Iraq

Author name: نافع فرحان مفتن الفرحان

Supervisor name: منال بادي التميمي | خالد كاطع الفرطوسي

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Thi-Qar - College Of Science - Department Of Biology

Language: English

University location: Dhi Qar

First pages:

التحري عن الفطريات الانتهازية لدى مرضى داء السكري في محافظة بابل

Author name: فاضل عباس راضي

Supervisor name: منعم مكي الشوك | وداد ناجي جعفر

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science - Department Of Biology

Language: Arabic

University location: Babylon

First pages:

المعالجة الفطرية لبعض الملوثات المطروحة في مبزل شركة الفرات العامة للصناعات الكيمياوية في بابل/ العراق == Mycoremedation For some disposal pollutants toAL-forat general Company for chemical industries drainage Babylon\Iraq

Author name: ايات رحيم خلف حسين

Supervisor name: نداء شهاب حمد

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Babylon - College Of Science For Girls - Department Of Biology

Language: Arabic

University location: Babylon

First pages:

دراسة تاثير الاصابة بطفيلي Toxoplasma gondii على مستوى هرمون الكورتيزول كمؤشر للاجهاد عند المرضى النفسيين في بعض مستشفيات بغداد == Study the Effect of Toxoplasma gondii infection on Cortisol levels and Stress Index in Psychiatric Patients Referred to Some Hospitals in Baghdad

Author name: نور حمزة فائق الزبيدي

Supervisor name: هناء كامل حمد | احسان مهدي الصقر

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad - College Of Science For Girls

Language: Arabic

University location: Baghdad

First pages:

بعض الجوانب المناعية لالتهاب المفاصل الجرثومي المتسبب عن متحلل ودناStaphylococcus aureus في الجرذان البيض == Some Immunological Aspects Of Septic Arthritis Caused By Staphylococcus Aureus Lysate And It'S DNA In Albino Rats

Author name: سارة سمير سعيد مال الله

Supervisor name: نوال محمد عتبة | حارث جبار فهد المذخوري

General topic: Biology

Specific topic: Microbiology

Degree: Master

University: University of Baghdad

Language: English

University location: Baghdad

First pages: