Author name:
صفا احمد عبد القادر محمد دنيدل
Supervisor name:
صاحب جمعة عبد الرحمن | عويد هيدان الخزرجي
Abstract:
هدفت الدراسة الحالية الى معرفة تاثير المستخلص الكحولي لنبات ذيل العقرب Heliotropium europium في وزن الجسم وعدد من المتغيرات الفسلجية والكيموحيوية كتركيز الهيموكلوبينHaemoglobin (Hb) وحجم كريات الدم المرصوصةPacked cell volume (PCV) والعدد الكلي لخلايا الدم | The aim of the present study was to investigate some effects of the alcoholic extract of Heliotropium europium in body weight and some of physiological and biochemical parameter such as concentrations of Hemoglobin (Hb), Packed Cell Volume (PCV) and the total count of leukocytes (WBCs) and concentrations glutathione (GSH), Malondialdehyde (MDA), Peroxynitrite radical (ONOO - ) , Superoxide dismutase enzyme (SOD), glucose, Total Cholesterol (TC), Triglycerides (TG), High density lipoprotein (HDL - C), Low density lipoprotein (LDL - C), Very Low density lipoprotein (VLDL - C), Atherogenic indices levels, Total protein, Albumin, Globulin, Urea and Uric acid in serum and histological changes in liver of male rats that exposed to oxidative stress induced by hydrogen peroxide H2O2(0.5%) with drinking water for (30) days, and comparison these effects with the effects of the vitamin C as well known antioxidants(250 mg/kg of body weight), for the purpose of this study preliminary experiments were carried out to find the most effective does of plant extracts and it was found that this does was (300 mg/ kg) body weight.The study included(35) male albino rats, (4 - 6) months ages and(245 - 280)gm weight were divided to (7) groups both group with (5) rats as follows : Control group, hydrogen peroxide (H2O2) group, Heliotropium extract group, H2O2+ Heliotropium extract group, Heliotropium extract + vitamin C group, H2O2+ Vitamin C group, H2O2+extract of Heliotropium+ vitamin C group.The results showed : 1. The animal under oxidative stress by hydrogen peroxide caused a significant decrease (p? 0.05) in body weights and values of Hb , PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin, globulin and compared with the control group. While it caused a significant increase in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels(I, II, III) and urea compared with the control group.2. The treatment of animals exposed to oxidative stress induced by hydrogen peroxide with extract of Heliotropium europium showed significant increase in the weights of animals, Hb, PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin and globulin compared with a hydrogen peroxide group. While this treatment showed significant decrease in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels (I, II, III), and urea when compared with a hydrogen peroxide group.3. Compared with a hydrogen peroxide treatment of rats exposed to oxidative stress by hydrogen peroxide with vitamin C, extract of plant and vitamin C with hydrogen peroxide group showed the results were similar to the results of treatment plant extract with hydrogen peroxide group.4. The treatment plant alcoholic extract and vitamin C showed a significant increase in the level GSH, MDA, activity of SOD enzyme, HDL - C, globulin compared with the control group. And showed a significant decrease in animal weights, WBCs, ONOO. - , LDL - C, VLDL - C and urea compared with a hydrogen peroxide group. While not shown significant differences in the values of Hb, PCV, glucose, cholesterol, TG, Atherogenic indices levels total protein and uric acid compared with the control group.5. The treatment alcoholic plant extract group showed increase in the weights of animals and values Hb, PCV, WBCs, GSH, MDA, Triglyceride, VLDL - C, Atherogenic indices levels, globulin and urea compared with the control group. Showed significant decrease in the HDL - C and albumin compared to the control group. while did not show significant difference in ONOO. - , activity SOD enzyme, glucose, cholesterol, total protein and uric acid compared the control group.6. The most important histological changes that have occurred in this study have showed treatment hydrogen peroxide compared with a control to swelling hepatocytes with necrosis each and the small size of the nuclei with swelling sinusoid, and the treatment of animals exposed to oxidative stress plant extract has showed a lack of clarity within the cells in hepatocytes necrosis and swelling sinusoid compared with a hydrogen peroxide while the treatment of animals exposed to oxidative stress with vitamin C showed sinusoid constriction and showed treatment alcoholic plant extract and vitamin C for the animals exposed to oxidative stress loss nucleus cells with the difficulty of distinguishing sinusoid.
