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نسبة الكلوبيولينات المناعية عند اصابات بكتيرية مختلفة == Immunoglobulins ratio in a different bacterial infections

اسم المؤلف: سجاد كاظم حسين الفحام
اسم المشرف: سيوف خومان علوان
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:

تاثير حامض الجبرليـك (GA3) على النمو الخضري لنبات البصل Allium cepa == The Effect Gibbrlic Acid on the vegetable growth Allium Cepa

اسم المؤلف: عباس عبد نور هادي الجبوري
اسم المشرف: انتصار حسين مهدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:

دراسة نوعية للبكتريا السالبة لصبغة كرام المعزولة من بيئة واخماج مستشفى عفك العام == Qualitative Study of Gram Negative Bacteria Isolated from Environment and Infections of Afak General Hospital

اسم المؤلف: زينب عبد الكريم عليوي الفرحاني
اسم المشرف: علي عبد الرحيم الناشي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة جمع 400 عينة سريرية وبيئية من مستشفى عفك العام في محافظة الديوانية للفترة من 1/11/2012 ولغاية 31 /3/2013 لغرض دراسة نوعية البكتريا السالبة لصبغة كرام والملوثة لبيئة واخماج المستشفى ومدى حساسية العزلات البكتيرية ومقاومتها للمضادات الحيوية والمطهرات شائعة الاستخدام .اظهرت هذه الدراسة ان 236 عينة وبنسبة 59% قد اعطت نتيجة موجبة للفحص البكتيري للبكتريا السالبة لصبغة كرام توزعت بين 31 عينة من مصادر سريرية وبنسبة 56.37 % و206 عينة من مصادر بيئية وبنسبة 59.43% بينما كانت 164 عينة وبنسبة 41% سالبة للفحص البكتيري للبكتريا السالبة لصبغة كرام توزعت بين 24 عينة من مصادر سريرية وبنسبة 43.63% و140 عينة وبنسبة 40.57 % من مصادر بيئية من مستشفى عفك العام .كانت العزلات البكتيرية السالبة لصبغة كرام المشخصة والبالغة 236 عزلة قد توزعت على اربعة اجناس وهي Pseudomonas شملت نوعين : P.aeruginosa 12.7% ، P. flourescens 8.5 % ، Klebsiella شملت K. pneumoniae 10.5% ، K.oxytoca 10.5% ، Serratia marcescens 3.4% ، Acenitobacterشملت A. baumanni 2.1% ، A.lwoffii 1.2% ، A.calcoceticus 0.8 %، Escherichia شملت E. coli 45.5% ، E.hermanni 4 % . كانت نسبة التلوث البكتيري في العينات السريرية الحروق والجروح قد بلغت 53.3 ، 60 % على التوالي ، بينما التلوث البكتيري في العينات البيئية المتمثلة بردهات دخول المرضى ، المطبخ ، صالات العمليات الجراحية ، ردهات الخدج وملابس العاملين في المستشفى قد شكلت النسب 66.6 ، 53.8 ، 60 ، 54.6 ، 40% على التوالي . كما اختبرت حساسية العزلات البكتيرية السالبة لصبغة كرام اتجاه 13 مضادا حيويا وقد اظهرت العزلات البكتيرية مقاومة وحساسية مختلفة تباينت حسب نوع العزلات البكتيرية وطبيعة المضاد الحيوي .كانت اعلى مقاومة اظهرتها بكتريا K.pneumoniae تليها K.oxytoca اتجاه مضاد Tetracycline اذ بلغت النسبة 96% و92% على التوالي . كما تميزت كل من بكتريا E.hermanni ، A.baumanni بمقاومتة عالية اتجاه ال Gentamycin اذ بلغت 80.3% ، 80% على التوالي اما P.aeruginosa فقاومت ال Tobramycin بنسبة عالية بلغت 90 %، بينما تميزت جميع البكتريا المعزولة في هذه الدراسة بحساسيتها اتجاه مضاد ال Imipenem . اما اختبار حساسية العزلات البكتيرية اتجاه المطهرات فقد ابدت مقاومة متفاوتة حسب نوع العزلات البكتيرية وتركيز وطبيعة المطهر وشملت المطهرات المستخدمة في التضاد الجرثومي كلا من الفورمالين والبوفيدين - ايودين والهبتين والديتول حيث كانت اعلى مقاومة اظهرتها E.coli اتجاه مطهر الفورمالين بتركيز 12.5% اذ بلغت 93.4% . درست بعض عوامل الضراوة المنتجة من الانواع البكتيرية ومنها انتاج المحفظة فوجد ان عزلات كل من بكتريا A. baumanni، P. aeruginosa ، K. pneumoniae وE.coli منتجة للمحفظة وبنسبة 40 ، 33.33 ، 100 ، 23.36 % على التوالي ، اما انزيم الهيمولايسين فوجد ان جميع عزلات A.baumanni غير منتجة لهذا الانزيم في حين جميع عزلات P. aeruginosa كانت منتجة لهذا الانزيم بنسبة 100% ، اما انزيم البروتيز فوجد اعلى نسبة لانتاجه في P.aeruginosa بنسبة 100 % وادنى نسبة في بكتريا E.coli وبنسبة 14 % ، كانت اعلى نسبة لانتاج انزيم الجلاتينيز من قبل كل من A.baumanni وP.aeruginosa وبنسبة 100 % وتميزت عزلات K.pneumoniae بعدم القدرة على انتاجه . | The study included the collection of 400 clinical specimens and environmental from Hospital Afak General in the province of Diwaniyah for the period from 1/11/2012 until 3/31/2013 for the purpose of study quality the gram negative bacteria and contaminated environment and hospital infections and the sensitivity of the bacterial isolates and their resistance to antibiotics and antiseptics commonly used . This study showed that 236 sample and 59% gave a positive result for the examination of bacterial for bacteria gram negative distributed among the 31 sample sources of clinical and increased by 56.37% and 206 samples from environmental sources and increased by 59.43% while the 164 sample and 41% negative for screening bacterial for bacteria gram negative distributed among the 24 samples from clinical sources, and by 43.63% and 140 sample and by 40.57% from environmental sources of Afak General Hospital. The bacteria gram negative isolates diagnosed, amounting to 236 isolation has distributed over four genera Pseudomonas They included two types : P.aeruginosa 12.7%, P. flourescens 8.5%, Klebsiella included K. pneumoniae 10.5%, K.oxytoca 10.5%, Serratia marcescens 3.4% , Acenitobacter included A. baumanni 2.1%, A.lwoffii 1.2%, A.calcoceticus 0.8%, Escherichia included E. coli 45.5%, E.hermanni 4%. The percentage of bacterial contamination in clinical samples of burns and wounds had reached 53.3,0.60% respectively, while bacterial contamination in environmental samples of lobbies entry of patients, the kitchen, Eat surgeries, lobbies premature and clothes of hospital personnel had formed ratios of 66.6, 53.8 0.60 , 54.6 0.40%, respectively. The sensitivity of the bacteria gram negative isolates tested for direction 13 have shown antibiotic - resistant bacterial isolates and different sensitivity varied by type of bacterial isolates and the nature of the antibiotic. The highest resistance exhibited by the bacteria K.pneumoniae followed K.oxytoca direction Anti Tetracycline as the percentage reached 96% and 92%, respectively. It also marked by both bacteria E.hermanni, A.baumanni resisting the trend of high Gentamycin reaching 80.3%, 80%, respectively, and P.aeruginosa resisting the Tobramycin high proportion reached 90%, while all characterized bacteria isolated in this study sensitivity counter direction the Imipenem . The test of the sensitivity of the bacterial isolates direction disinfectants have shown resistance varying according to the type of bacterial isolates and the concentration and nature of the cleanser included disinfectants used in contrast bacterial both Formalin and Povidin - Iodine and Hibtin and Dettol had the highest resistance exhibited by E.coli direction antiseptic Formalin concentration of 12.5% reaching 93.4% . I studied some of the virulence factors produced by bacterial species, including the production of the wallet and found that all isolates of bacteria A. baumanni, P. aeruginosa, K. pneumoniae and E.coli producing capsule and by 40, 33.33, 100, 23.36%, respectively, while the enzyme was found haemolysin that all isolates A.baumanni unproductive for this enzyme, while all P. aeruginosa isolates were producing this enzyme by 100%, while the enzyme protease and found a higher proportion of production in P.aeruginosa 100% and the lowest percentage in the bacteria E.coli and 14%, the highest percentage for the production of an enzyme gelatinase by both A.baumanni and P.aeruginosa and 100% K.pneumonae isolates were characterized by the inability to produce it

دراسة تشخيصية جزيئية ودمية وكيموحيوية لانواع الاكريات Eimeria spp. في الدجاج المصاب طبيعيا وتجريبيا في محافظة الديوانية == Molecular Diagnosis Study And Haematological And Biochemical of Coccidiosis Eimeria Spp. In Natural And Experimental Infection Chicken In Al - Diwanyia City

اسم المؤلف: خالد ثامر مطر الشيباني
اسم المشرف: خيري عبد الله داود العكيلي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في البيت الحيواني التابع لقسم علوم الحياة / كلية التربية / جامعة القادسية للمدة من 1/9/2013 ولغاية 1/9/2014، للتحري عن انواع طفيلي الاكريات المتطفلة على الدجاج المصاب طبيعيا وعزل الطفيلي من الحالات المصابة الواردة الى المسستشفى البيطري | The study conducted in The animal house of the Biology department, College of Education, University of Al - Qadisiyah during the period from 1/9/2013 to 1/9/2014. The study diagnosed the species of Eimeria parasites in naturally infected chickens in poultry farms. Eimeria oocysts was isolated from the infected chickens feaces in Veterinary hospital and Veterinary clinics.Then, the cases diagnosed initially in the laboratory by direct examining stool smears and the positive cases were recorded.Through this study, oocysts were isolated, and two species of Eimeria parasites were diagnosed (E.tenella and E.maxima) by using the technique of Conventional Polymerase Chain Reaction technique.There are 186 cases of infected chicken which examined, and the results showed that (186 out of 315)stool Samples(59%), include infected with two species of Eimeria parasites E.tenella was in (135 out of 186, 72.5%) while the species E.maxima was in(51 out of 186, 27.4%). The experimental study was conducting two experiments were done.The first experiment, depend on the experimental infection of E.tenella species which was used for two types of chicken, (Ross type which is foreign, and local breed).The second experiment, includes experimental infection with the species E.maxima also in two types of chickens that mention above. Symptoms and clinical signs of the first experiment on foreign chickens included were recorded it is noticed that infected the chickens with E.tenella have was dull, unorganized feather and loss of appetite in addition to bloody diahrreha, loss weight, emaciated and isolated. The Gross Lesions include congestion of cecum and an increase in their sizes while inside the cecum, many ulcerated lesions were noticed, filling cecum with mucus and blood, liver was congested and enlarged. Microscopicale examination recorded congestion was full with blood cells and necrotic tissues cecum and falling of cells in the cecum lumen. Many different stages of the parasites were noticed as schizonts, merozoites, gametes and oocysts Hematological parameters showed high significant decrease (p?0.05) in Hb (0.38±5.94 mg / 100ml) in the fourth week of infection, PCV (0.68±18.47%) in the fifth week and RBC was (1.58±0.02x106) in the fourth week. Also, the infection led to significant increase difference of WBC (30.19±0.95x103) in the seventh week of infection in comparison of uninfected chickens of the control sample. The biochemical parameters of blood serum showed that significant decrease (p?0.05) in glucose concentration (156.98±1.75mg / 100ml in the fourth week of infection), cholesterol concentration and total protein concentration (62.11±4.40, 1.92±0.92 mg / 100ml respectively in the fifth week of infection).Also, Uric acid increased significantly (p?0.05) to reach(10.19±0.52mg / 100ml) in the fourth week of infection in comparison of uninfected chickens of the control group. Average weight was showed the significant decrease (p?0.05) of the chickens quantity (368.88±3.01gr in the fourth week of the infection), and an increase the percentage of mortality(23.33±0.23% in the fifth week of infection), addition to the decrease of consuming food by bird (282.8±2.19gr in the fifth week of the infection) in comparison of uninfected chickens of the control group. While the results of local breed chickens in the first experiment were less severity than that with the foreign type (Ross) but the colour of the stool was brown mixed with blood. The Gross Lesions showed pathological changes in the cecum as congestion and bleeding. Moreover, ulcers areas were smaller and less in number in comparison with the infected foreign chickens by the same parasite. The microscopic pathological changes of E.tenella in the cecum showed hyperplasia in goblet cells and congestion of blood capillaries with less number of stages of the parasite in comparison with the foreign chickens. The hematological parameters showed significant decrease (p?0.05) in Hb concentration and percentage of PCV (7.13±0.31mg/100ml, 21.26±1.82% respectively in the fifth week of infection), while the RBC count was (2.05±0.12 x 106 in the fourth week of infection and significant increased (p?0.05) in WBC count was (30.63±0.38 x 106 the sixth week of infection) in comparison with the chickens of the control group. The biochemical parameters of blood serum showed the significant decrease (p?0.05) in glucose concentration (169.60±2.16mg/100ml in the fifth week of infection), cholesterol concentration and total protein concentration (70.89±2.01, 2.25±0.39mg/100ml respectively in the fourth week of infection). Uric acid increased significantly (p?0.05) to reach (7.62±0.90mg/100ml) in the fifth week of infection in comparison with the control group. Average of the weight was significant decrease (p?0.05) (190.28±3.81gm), high increase in the average of mortality (10±0.20%), and high decrease in the consuming food average (413.4±3.91gr) in the fourth week of infection in comparison with the uninfected control group. The second experiment of the foreign chicken which infected experimentally by E.maxima showed asymptoms and clinical signs were nearly similar in the foreign chickens that infected with E.tenella, but mild in severity is average. The symptoms include fatigue, fallen wings, loss of appetite, mucus diarhea mixed with blood. The Gross Lesions showed severe changes in the infected part of intestine as congestion and filling of intestine with mucous secretion of bad odour. While the microscopic pathological changes showed the tissues section of intestine were damaged, necrosis of intestine villi due to the large number of different stages of the parasite with hyperplasia of goblet cells. The hematological parameters showed significanty decrease (p?0.05) in Hb concentration, percentage of PCV and RBC count (7.55±0.61mg/100ml, 23.62±0.81% and 2.08±0.10x106 in the fourth week of infection) and significant increased (p?0.05)in WBC count (31.26±0.63x106 in the seventh week of infection) in comparison with the uninfection control group. The biochemical parameters of blood serum showed that there was the significant decrease (p?0.05) in glucose concentration (164.86±2.11 mg /100ml in the fifth week of infection), cholestrol concentration and total protein (77.14± 3.38, 2.24±0.36 mg/100ml) and significant increased (p?0.05) in Uric acid concentration (8.20±0.79mg/100ml) in the fourth week of infection in comparison to the control group. The results of productive characteristics refered to significant decrease(p?0.05) in the weight of chickens (479.28±0.23gr) and significant increase (p?0.05) in mortality (10±0.11%) during the fifth week of infection. Also, there was significant decrease (p?0.05) in the consumed food by birds (368.5±2.10gr) during the fourth week of infection in comparison to the chicken of the control group. While the results of local breed chicken in the second experiment, the symptoms and clinical signs showed no sign of disease, and the chickens were active in spite of presence of infection and the releasing of Oocysts. The Gross Lesions showed the inflammation and congestion, onlargment of the infected part of small intestine, little mucous and liquid inside the lumen.As far as microscopic changes are concerned, the tissue examination showed some necrosis and damage of the villi ends and an increase in the size of the goblet cells. Hematological parameters showed to the significant decrease (p?0.05) in Hb concentration and percentage of PCV (7.86±0.45 mg/100ml, 23.60±0.53% respectively during the fifth week of infection), RBC count (2.10±0.13 x 106 in the fourth week of infection) and significant increased (p?0.05) in WBC count (32.98±0.58 x 106 during the sixth week of infection) in comparison with the control group. The biochemical parameters of blood serum showed that there was the significant decrease (p?0.05)in glucose concentration and cholestrol concentration (170.60 ±2.01, 79.70±2.69 mg/100ml respectively in the fourth week of infection), total protein concentration (2.42±0.31mg/100ml in the fifth week of infection) and significant increased (p?0.05) in Uric acid concentration (6.52±0.82mg/100ml in the fourth week of infection) in comparison to the uninfection control group. Average of the weight referred to significant decrease (p?0.05) of chickens (213.16±3.12 gr) and consuming food (481±3.21 gr) during the fourth week of infection.While there were no differnces in the percentage of mortality comparison with the uninfected chicken of the control group. Also, the results of the number of released oocysts showed significant increase (p?0.05)in number of released oocysts with stool by infected foreign ckicken(Ross) in comparison with the local breed chicken which infected by the same parasite.Also, the number of the dropped oocysts of E.tenella were higher than that of E.maxima.

دراسة ادلة التنوع الاحيائي لتقييم مجتمع العوالق الحيوانية في نهر الكوفة - العراق == A Study of Biodiversity Indices To Assessment The Zooplankton Community In The Kuffa River - Iraq

اسم المؤلف: حسين عليوي حسن الكرعاوي
اسم المشرف: مهند رمزي نشات
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة على نهر الكوفة في وسط العراق والتي هدفت الى التعرف على التنوع الاحيائي للعوالق الحيوانية من خلال قيم بعض ادلة التنوع الاحيائي وكذلك والتعرف على نوعية المياه من خلال قياس بعض الخصائص الفيزيائية والكيميائية لمياه النهر وكذلك من خلال قيم دل | The Present study was carried out on the AL - Kuffa River in the middle of Iraq to know biodiversity of zooplankton by through some of biodiversity indices values and to know water quality by through measurement some physical and chemical characteristics of water as well as by through the Canadian Water Quality Index (CCME WQI) values. The samples of the study have been collected monthly for water and zooplankton for the period from March 2012 till February 2013 at four selected stations in the river; the first is located in Al - Kufel city, the second in Al - Kuffa city, the third in Aboskeer city and the four after Al - Mushkab city. The study was achieved by measurement of some physical and chemical properties of water were analyzed based on the importance of these parameters the parameters studied included Air temperature, water temperature, pH, water current velocity, turbidity, total suspended solids, electrical conductivity, salinity, total dissolved solids, dissolved oxygen, percentage of saturation, biological oxygen demand, total alkalinity, total hardness, calcium, magnesium, chloride, sulphat, phosphate and nitrate. The study also included biological aspects using density, Rrelative abundance index (Ra), Constancy index (S), Species richness index (D), Shanon - Weiner index (H), Species uniformity index (E) and Jaccard presence - community were also employed to determine the values of species composition, density and zooplankton biodiversity in the study area. The results showed clear consensus from air temperature and water temperature in all study locations.Air and water temperature ranged from 6 to 41 ?C and 7 to 34?C respectively, the Water current velocity values were fluctuated according to the different water levels during the study period and ranged from 0.19 to 0.71 m/sec, while the (TSS) values were low and ranged from 3.54 to 52.05 mg/l, and turbidity 1.69 to 55.76 NTU, the pH values were neutral tend to light alkaline and ranged was ranged from 7.1 to 8.7, The electrical conductivity values varied from 810 to 1810 ?s/cm, according to the values of salinity 0.51 to 1.158 ‰ the water of the river was classified as Oligohaline, (TDS) showed medium values ranged from 440.4 to 977 mg/l. This study showed that river were well oxygenated the dissolved oxygen values ranged from 5.5to12.2mg/l and high percentage of saturation recorded from 72 to 148 %, The study recorded values to the BOD5 were ranged from 0.9 to 6.4 mg/l.The study showed that the water of the river was classified as slight alkaline and bicarbonate ions were dominant throughout the study period according to the values of total alkalinity from110 to 210 mg CaCo3/l, and the river water classified as very hard according to the total hardness values from 237.9 to 538.2 mg CaCo3 /l., While the concentrations of calcium and magnesium were from 73.19 to134.1 and from10.46 to 55.59 mgCaCO3/L respectively, The chlorid and sulfate values were from117.6 to 244.5 mg/l and136.9 to 528.14mg/l respectively, The nutrients showed clear fluctuation in their concentrations, nitrate values were varied from 3.55to36.43?g/l and phosphate values were from 0.15 to 2.89 ?g/L.. In the current study about 164 Taxonomic units of zooplankton were identified, the rotifer was dominate group including 92 taxa to rotifera, 35 taxa belonging to cladocera and 37 taxa to copepod. The Zooplankton showed high density in river 29470 ind /m3 during April 2012 while lower1100 ind/m3 in December 2012 also the rotifer recorder density from 425 to 17925 ind/m3, cladocera density from 25 to 4850 ind/m3 and copepod density from 300 to 15450 ind/m3 the high values were in spring and autumn while lowest values were recorded in summer and winter. The results of relative abundance index showed that the species rotifera : Keratella cochlearis, K.valga, Euchlanis delatata while the cladocera Alona rectangular, Bosmina longirostris, Chydorus sphaericus, Simocephalus vetulus and the copepods : nauplii were more abundant in the kuffa river. also the results of Constancy index showed fourteen taxa belonged to zooplankton which were considered "Constant" at stations However the other taxonomy units varied from "accessory" and "accidental" taxonomy units in study stations he Values of the index of Species richness of Rotifer varied from 3.42 to18.26 and Cladocera varied from 0.9 to8.13 and Copepod from1.46 to 6.13 with greatest values were recorded in April While lower values in September and June The highest percentage of similarity 70.58% for Zooplankton were reported from stations 3 and 4 in and lowest similarity reaching 50.30% from stations 2 and 3. the Shanon - Weiner index of total Zooplankton varied from 1.81 to 4.13 bit/ind while the Shanon index of Rotifer from 1.85 to 3.78 bit/Ind, Cladocera varied from 0.001 to 3.03 bit/Ind and Copeoda varied from 0.35 to 2.71 bit/Ind with greatest values were recorded in April 2012 and September 2012 While lower values in and June 2012 and January 2013, The uniformity index of Rotifer varied from 0.01 to 0.9, Cladocera from 0.01to 1 and Copeoda from 0.14 to 0.88 These high values indicate that there is no ecological stress on zooplankton in the study area. The results of the Canadian Water Quality Index (CCME WQI) for aquatic life showed that the water quality of River Kuffa in the study area obtained good to marginal level (90.93 - 62.76) the highest value was recorded at station 1 through April 2012 and lowest value was recorded at station 2 through July 2012

استخدام التراكم الحيوي للعناصر الثقيلة في انسجة الديدان المتطفلة على نوعين من اسماك المياة العذبة في محافظة القادسية == The Usage of Bio - Accumulation of Heavy Meyals In Tissues of Parasitic Helminthes of Two Types of Fresh Waters Fishes In Al - Qadisiya Governorat.

اسم المؤلف: عاصفة مطرود ياسين المياحي
اسم المشرف: هادي مدلول حمزة الميالي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية في اربعة انهار ضمن محافظة القادسية هي نهرالديوانية والشامية والحمزة وعفك, اذ جمعت 945 سمكة تعود لنوعين من الاسماك هما الشبوط الاعتيادي Barbus grypus والخشني Liza abu وبعــد فحصها وجـد ان 205 سمكة مـن اسماك الشبوط الاعتيادي و344 سمكة | The present study has been conducted in four parts of AL - Qadisiya (Diwaniya center, Shamiya, Hamza and Afaq). A sample of 945 fish of Barbus grypus and Liza abu has been examined. It was found that 205 of the Barbus grypus fish and 344 of the Liza abu were infected with parasitic intestinal worms. Some parasitic intestinal worms have been detected such as the Neoechinorynchus iraqensis in both fish types. The female paulisentus in Barbus grypus, Bothriocephalus acheilognathi were found in both Barbus grypus, Liza abu and the larva of Contracaecum spp was seen in the Liza abu and Barbus grypus type. In Diwaniya river had high infection of both fish types was 61.31%. The Barbus grypus fish were recorded with 70.11% while the Liza abu fish infection was 57.21%.. In Hamza river the total percentage of infection was 56.79 % in both types. The Liza abu infection in the four parts of Diwaniya as 56.30%. In September the infection percentage was higher by 63.21% in the two kinds of fish. while in February, December the infection rate was as low as 50%. It was also found that length categories of (26 - 24.1)cm of Barbus grypus had high percentage of 67.16%, while the length category (28 - 26.1) cm suffered 64.47% infection with intestinal worms in the length category (> - 30.1) was 22.22%.As for the Liza abu it had a high percentage of infection in length category (12 - 10.1) cm, the percentage was 67.53% and dropped to 46.53% in length category (8 - 6)cm.The present study has discovered many pathological changes (visable and microscopic)caused by the intestinal worms, Among the visable changes was embolism and bleeding of the walls of the infected intestinal microscopic changes included an increase in the phlogistic and phagocytic cell and ruining of the internal tissue lining the intestines and atrophy villi.Eight heavy metals were measured.They included Cadmium, Lead, Zinc, Nickel, Copper, Manganese, Mercuryand Chromiumby means of an atomic absorption spectrophotometer tissues from the a forementioned types of fish were measured for these heavy metals these tissues were from the liver, muscles, gills, two kinds of intestinal worms (Cestode and Nematode) were measured also water and sediments (deposits) from four districts of Diwaniya throughout the year, Cadmium registered high concentrations in autumn (12.67) Mg/ gm in the liver of the Barbus grypus. lower concentrations of Cadmium were registered in Winter (1.30) Mg/ gm in the liver of Liza abu.The Barbus grypus livers had high concentrations of lead in autumn (21.03) Mg/ gm in Hamza rivers, where as gills of the Liza abu registered lower concentrations(6.43) Mg/gm in spring.The livers of the Barbus grypus registered high concentrations of zine in autumn which where (32.27)Mg/ gm in Hamza river. Shamiya river they dropped to 21.17 Mg/ gm in spring, It was observed that Nickel had high concentrations in the livers of both fish types, The highest of which was in autumn, the the Hamza river had the highest concentrations in autumn which were (29.17, 28.63, 27.67) Mg/gm.In spring the nickel concentrations were low in the gills of the Liza abu11.10 Mg/ gm in Shamiya river, The livers of the Barbus grypus had the most concentrations of copper which reached 2.02 Mg/ gm while livers of the Liza abu had 1.92 Mg/ gm concentrations in autumn in Hamza river.Manganese showed low concentrations in the Liza abu gills in winter and spring they ranged (1.67, 1.73) in Shamiya river, manganese concentrations increased in autumn and summer to reach (14.83, 10.07) Mg/ gm in the Liza abu liver and (15.13, 10.57) Mg/ gm in the muscles of the Barbus grypus in Hamza river, Marcury had the lowest concentrations 24.37 Mg/ gm in the gills of Liza abu in Afaq river, It was observed that the livers of the Barbus grypus were more vulnerable, the highest concentrations was registered 49.97 Mg/ gm in Hamza river followed by Diwaniya which registered 48.97 Mg/m in autumn. Chromium registered lowest concentrations compared to the other metals,.cestodes isolated from the Barbus grypus registered high of mercury concentrations that reached 37.667 and 37.300 Mg/ gm in Hamza and Diwaniya rivers, chrome concentrations was low in the tape worms 0.166 Mg/ gm while nematodes, the concentrations was 0.157 Mg/ gm in Shamiya river.cestoda and nematodais isolated from the intestines of Liza abu registered high concentrations in autumn, cestoda had the most of the concentrations of heavy metals, Mercury and nickel had high concentrations of (35.783, 25.840) Mg/gm, copper and chrome had lower concentrations(1.881, 0.174) Mg/gm inHamza river, nematode registered high concentrations that reached 33.994 Mg/ gm in mercury in Hamza river.As for the rivers sediments Autumn was the most effected by heavy metals for the sediments in Hamza river had high concentrations in Manganese, mercury, and chrome (367, 376.97, 290.93) Mg/ gm respectively while the lowest concentrations was for copper 22.53 Microgram/gram in Shamiya river in spring. The rivers water registered low concentrations of heavy metals in spring, The Shamiya river had low concentrations of lead and cadmium (80.57, 54.60) Mg/gm, autumn registered higher concentrations compared to spring where the highest concentration of manganese and Copper was (512.37, 442.83) Mg / gm in Hamza river.

عزل وتشخيص الفطريات المصاحبة لاخماج الجيوب الانفية وحساسيتها لبعض المضادات الفطرية == Isolation And Identification of Sinusitis Infections Borne Fungi And Its’ Sensivity To Antifungals

اسم المؤلف: رواق حيدر عبد النبي المحنة
اسم المشرف: ماجد كاظم عبود الشبلي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: جمعت ((97 مسحة انفية من المرضى المصابين باخماج الجيوب الانفية, ولكلا الجنسين وباعمار مختلفة والذين راجعوا العيادات الخاصة بالانف والاذن والحنجرة Ear Nasal Throat في مدينة الديوانية للفترة من تشرين الثاني 2012 الى نيسان 2013, لغرض عزل وتشخيص الفطريات المسب | A total of 97 nasal swab were collected from patients with sinusitis, for both sexes with different ages who attended private clinics of Ear Nasal Throat in the city of AL - Diwaniya, for the period from November 2012to April 2013, for the purpose of isolating and diagnosing fungi that cause inflammation of the sinuses and test their sensitivity toward some antifungal as well as the study of the effect of some factors on the infictions rats, which include age, gender, housing, and professions and relationship of chronic diseases. Specimen were cultivated on culture media SDA to determine the types of fungi responsible the infection, it is found that the samples gave positive growth were about 36 at a rate of 37.11 %, while the samples which gave a negative growth were about 61 at a rate of 62.88 % Note that some samples give more than one fungal type so amounted to 44 the number of fungal infections and 45.36 %.The isolated fungi included and their rates Aspergillus flavus13.63%, Aspergillus niger 13.63 % , Aspergillus terreus 4.54 % , Pencillium sp 18.18 % , Blastomyces dermatitidis and Cladosporium sp 11.36 % of each one , Alternaria sp and Trichophyton sp 4.54 % of eachone , Troulopis sp , Mucor sp , Rhizopus sp, Paecilomyces sp, Rhodotorula sp, Ulocladium sp, Geotrichium sp and Bipolaris sp 2.27% of each one. The presnt study showed that females cases are more than males cases, where the percentage of female infection about 63.63% but male infection rate of about 36.36%, patients ranged in age between 11 - 76 years and found that the infection was more frequent among age groups 20 - 29 years 29.54% and less frequent among age groups 19 - 11 years 9.09 %. About the injury season shows that increase in the cold months of the year and less in the warm months, as it recorded the highest rate of infection during November at a rate of 43.18 %, while there have been no injury during April, as for Relationship of injury with housing areas of patients has been shown that the highest proportion of the injury people registered from urban areas 52.27 % compared to residents of rural areas 47. 72 %. The role of the professions in the incidence of injury has been shown that the highest recorded injury among housewives40.9%and the least among the unemployed 2.27%, while the relationship of chronic diseases has revealed the presence of 15 people suffering from chronic diseases and fungal infections arate of 15.45 %, the more chronic injuries were pressure disease, where they formed an injury rate of 40%.Results showed the inhibitory effect for antifungal Itraconazole, Ketoconazole, Econazole, Clotrimazole, Nystatin, Amphotericin B against 12 type of fungus Pencillium, Blastomyces dermatitidis, Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Cladosporium sp, Alternaria sp, Troulopsis sp, Mucor sp, Ulocladium sp, Geotrichum sp , Bipolaris sp , the antifungal Econazole was the most antifungal effective against isolated fungi and diameters of inhibition rate 30, 38, 20, 33, 30, 40, 35, 39, 27, 31, 19, 44? m of fungal species respectively, while the antifungal Amphotericin B show less effect against isolated fungi at arate of inhibition diameters 14, 19, 10, 10, 8, 12, 26, 18, 17, 22, 18, 21? m, respectively.As for the values of the MIC and MFC for Ketoconazole and Itraconazole for the fungi Aspergillus niger, Aspergillus flavus , Geotrichum sp found that antifungal Ketoconazole owned less MIC against all fungi, as it amounted to 0.24 Mg /ml of each one, while the MF Cagainst Aspergillus niger and Geotrichum sp was 7.8Mg /ml against Aspergillus flavus was 0.48 Mg /ml, while the antifungal - Itraconazole owned less MIC was 31.25 Mg /ml against all fungi, while the MFC was 62.5 Mg /ml against all isolates.The presnt study showed That fungal infections is one of the important causes of sinusitis infections and fungus Aspergillus spp is the most common.

دراسة مقارنة لعزلات بكتريا Staphylococcus aureus المعزولة من مياه النهر ومن حالات سريرية == A Comparative Study of Staphylosossus Aureus Bacteria Isolated From River Water And From Clinical Cases

اسم المؤلف: بنين مدلول امانة الزيادي
اسم المشرف: علي عبد رحيم الناشي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة جمع 120 عينة اخذت من مصدرين مختلفين, 60 عينة منها جمعت من بيئة مائية هي نهر الديوانية, بينما كانت 60 عينة سريرية جمعت من الاشخاص الراقدين والمراجعين لمستشفى الديوانية ومن الاصابات الجلدية المختلفة التي شملت الدمامل, الحصف, التقشر الجلدي, الخر | The study included assemble 120 samples which had been taken from two different sources, 60 samples collected from An aqueous environment which is Al diwaniya river, where the rest 60 samples have been collected from those people who were admitted to the Al diwaniya hospital and from of different ages from male and female. Where the environment examples collected from different places of riverbed Al diwaniya. The duration of collection samples has lasted for 3 months, started One handrad isolated had been diagnosed that proved its belong to a bacteria Staphylococcus aureus, 50 isolated where taken from environment isolate and the other 50 taken from clinical isolate depending on the diagnostic methods which included tests of farm, microscopic and biochemical, It was also confirm the diagnosis of bacterial using accurate diagnosis that uses device VITEK System tested sensitivity of bacteria isolate of S. aureus, against 12 selected antibiotic, environment and clinical cases has shown resistance and sensitivity of different varied by source isolate, the nature of the antibiotic and the resistance and sensitivity have been determined by measuring diameters of areas inhibition around tablets of antibiotics it used.All the clinical isolates showed a complete resistance for the antibiotic Lincomycin with 100% percentage, where it's resisted Pnicillin G with 92%, Nalidixic acid with 42%, while the highest resistance of environment isolates was 97% for Lincomycin. The clinical isolates showed a sensitively against the two antibiotics which are Cefamandol and Cephalothin, where their percentages reached 98% and 89%, respectively. Where the percentage of environment isolates reached for the same antibiotics 100% and 97% respectively. In general, the clinical isolates were more resisted for the antibiotics than environment isolates in overall rate.differed values of minimum inhibitory concentrationsmic MIC and the minimum bactericidal concentration MBC among each other with variation of clinical isolates from side and differed with environment isolates from another side while the values differed with environment and clinical isolates from third side, where the highest value reached of MIC against Polymyxin B is (20 - 120) and (100 - 240) mg/ml for environment and clinical isolates respectively, while the highest value of MIC that also was against Polymyxin B reached (40 - 140) and (130 - 250) mg/ml for environment and clinical isolates respectively. Where the value of MIC against Cephalothin reached (0.01 - 0.6) and (0.8 - 2.0) mg/ml for environment and clinical isolates respectively. Finally, the highest value of MBC also was against Cephalothin reached (0.08 - 0.9) and (0.9 - 3.0) mg/ml respectively. In general, the bactericidal and inhibitory concentrations of the clinical isolates are higher concentrations from those bactericidal and inhibitory concentrations for environment isolates.I studied the virulence factors that come from strains of S. aureus bacteria environment and clinical which included Capsula, enzymes of Coagulase that are associated and free, , Lipase, Hyaloronidase and B - Lactamase in addition to Haemolysin clinical isolates and Leucocidin and all the environment isolates are the highest percenage in the ability to form and possess virulence factors compared with environment isolates, where the highest percentage to produce virulence factors is belongs to the Haemolysin which reached 94% percentage in the clinical isolates where in the environment isolates reached 64% percentage followed by Leucocidin that reached 84% percentage in the clinical samples and 40% percentage in the In environmental samples.Also the percentage of blood Coagulation enzyme the associated and free is the highest percentage in the clinical isolates which reached percentage 42% and 38% for the associated and free respectively, where the percentage in environment reached 20% and 36% respectively and the virulence factors was the least presence in the environmental and clinical isolates is the portfolio that reached percentage of presence 22% and 12% respectively.

استعمال بعض انواع الطحالب والنباتات المائية في المعالجة الحيوية لمياه محطات المعالجة في مدينة الديوانية - العراق == Using of Some Dominant Algae And Aquatic Plants In Bioremediation of Waste Water From Wastewater Plants In Al - Dewaniya City, Iraq

اسم المؤلف: رائد كاظم عبد الاسدي
اسم المشرف: فؤاد منحر علكم | حسين يوسف الركابي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة المائية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على نوعين من المياه العادمة الصناعية المطروحة من مصنع نسيج الديوانية والمياه العادمة البلدية من محطة معالجة الديوانية، وللمدة من ربيع 2011 الى ربيع 2012 وهدفت الى اجراء مراقبة فصلية لنوعية المياه الداخلة لمحطتي المعالجة والخارجة منها | The current study has been conducted on two types of waste water, industrial wastewater from Al - Dewaniyah textile factory station1, station 2 and municipal wastewater from Al - Dewaniyah wastewater treatment plant station 3, station 4 in the City of Al - Dewaniyah - Iraq., for the period from Spring2011 to Spring 2012 and targeted monitoring quarterly the quality of water entering and leaving the stations.The study also includes the evaluation of the efficiency of some algae and aquatic plants to improve water quality via bioremediation of some pollutants in the water. The evaluation of water quality includes18 parameters as confirmed on spatial and temporal changes of these parameters, as well as the algal contents of these four stations(S1, S2, S3and S4). The results of physical and chemical analysis show the following ranges of : temperature (12.5 - 41.5°C), total dissolved solids TDS(692 - 1384.6 mg/l), total suspended solids TSS(58 - 277.4 mg/l), electrical conductivity EC(1225 - 2045µS/cm2), pH(7.0 - 7.8), dissolved oxygen DO(0.7 - 8.51mg/l), biological oxygen demand BOD(48.82 - 205 mg/l), total alkalinity (170.7 - 428 mg/l), total hardness TH (412 - 1623.3 mg/l), calcium (89.7 - 204.14 mg/l), magnesium (37.7 - 332.5 mg/l), nitrite (0.43 - 12.25 mg/l), nitrate(6.18 - 63.75 mg/l), phosphate (0.813 - 20.24 mg/l), lead (0.0983 - 1.076 mg/l), chromium (0.007 - 0.045mg/l), cadmium (0.0374 - 0.326 mg/l) and the total algal number(17.00 - 14202 individual/l).The spatial and temporal variations show significant differences at(P?0.05) among studied factors in the different stations.The qualitative study of algae reports that the dominance is to the blue green algae(Cyanophyta)by 46%, then is followed by diatoms(Bacillariophyta) by 26% down with Dinophyta3%, according to Shannon - Wiener index of diversity (H), the fall and winter classified as severely polluted all around the stations 0.377 and 0.669, respectively, while summer and spring are moderate pollution of a category (II) and the values of (H) is1.374 and 1.395 respectively, in situ has been observed that the stations are classified as highly polluted and have been ranked within the category(I) and the stations arranged according to the pollution as S3 <S4< S2< S1.According to Species richness index the first station S1 is the best, follows by S4, S3 while S2 comes the last in order, but in the terms of total abundance has topped the third station S3 among the others, then followed by S4, S2 and finally S1. on the other hand the current study includs laboratory investigation to demonstrate the efficiency of algae and aquatic plants to improve water quality as it has been experimenting with immobilization of algae technique, a new method has been used for the first time at the local level in the current study. The results show that the immobilized algae Stigonema sp.and Cladophora glomerata have lowered the pH of the treated wastewater which began relatively high, as well as increasing dissolved oxygen concentrations at the end of the experiment all transactions free and immobilized treatments , on the other side TSS has been reduced about 61.8% when treated with immobilized algae C.glomerata, nitrite has also been reduced at about 96.7% and 85.5% by Stigonema sp.and C.glomerata respectively, nitrate also has been reduced by 99.9% and 95.2% when treated with Scendesmus dimorphus and C.glomerata respectively, while the removal of phosphate reaches its peak when it has been treated with free Stigonema sp. and immobilized S.dimorphus by 99.92% and 98.35% respectively.The tested algae shows a great ability to reduce heavy metals, when immobilized alga Oscillatoria sp. removes about 94.27% of Lead, while free Stigonema sp. and C.glomerata are the best in Chromium removal about 92.42% and 92.35% respectively, and the C.glomerata is the best in Cadmium processing, a reduced rate of 99.21% followed by immobilized S.dimorphus of 97.13%. The current study also involves a laboratory experiment to evaluate the potential of two aquatic plants duckweed (Lemna minor L.)and water hyacinth Eichhornia crassipes Mart.(Solms.) in improving the quality of wastewater the tested plants have been worked at increasing the aeration of treated water by raising dissolved oxygen concentration about 85.26% and 84.4% for duckweed and water hyacinth, on the other hand the relative efficiency to reduce the pollutants by aquatic plants as follows : EC, NO2, NO3(31.66% and 28.45%, 55.7% and 50.01%, 55.7% and 13.3%)for duckweed and water hyacinth respectively, from another side water hyacinth is the most efficient in phosphate reducing at rate of 90.5% comparing with duckweed 49.78%.On the of heavy metals treatment the tested plants show a remarkable removal of lead 88.23% and 80.4% , chromium 97.84% and 95.18% for duckweed and water hyacinth respectively, with respect to cadmium, water hyacinth shows high efficiency in the removal of this metal by 98.14%, while duckweed is the least in the treatment of Cd by 81.8%. From the above results, the current study demonstrates that there are a spatial and temporal variations regarding the factors examined in the stations of wastewater treatment units and confirms that the efficiency of studied wastewater treatment stations is below the required level, which contributes in increasing the pollution of Al - Dewaniyah River, which is the unique source to supply the city with freshwater for all purposes, that receives large loads of pollutants.The study also confirms that the evaluation of the presence of algae in the studied stations.Thus the knowledge of algal composition could give an early warning and accurate assessment about the quality of sewage before dumping into river. In addition to the forgoing, it is concluded that the biological treatment (bioremediation) of wastewater using free and immobilized algae and aquatic plants have improved dramatically and tangibly the benefits of the quality of that water being a promising approach of the wastewater treatment as well as being low cost economically.

حركية بعض العناصر النزرة في النظام البيئي لنهر الديوانية == Kinetic of Some Trace Elements In The Ecosystem of Al - Diwaniya River

اسم المؤلف: حيدر مشكور حسين
اسم المشرف: باسم يوسف الخفاجي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The current study was conducted to understand the Relationship among concentration of selected of trace elements(Cadmium, Copper, Nickel, Lead and Zinc) in some trophic levels, water, and sediments, in Al - Diwaniya River. Samples were collected monthly for water, sediments, plant Phragmites australis (Cav.), phytoplankton, zooplankton, and two species of fish. from May (summer) 2013 up to April(spring) 2014 from three sites in Al - Diwaniya river. The current study included determine some physical and chemical parameters such as air and water temperature, pH, electrical conductivity, Total dissolved solids, Salinity, Light Penetration, Turbidity, Dissolved oxygen, the Biological Oxygen Demand, Total Organic Carbon of sediment, determined of the soil texture; and some trace elements in abiotic component such as water (dissolved and particulate), and in sediment, and so in biotic component included plant P. australis, phytoplankton, zooplankton, and two species of fish females (Cyprinus carpio) and (Tilapia zilli). The results showed that the air and water temperature ranged between (16.4039.55) ?C; (11.42 - 30.57) ?C respectively. The study also demonstrated that the pH values were neutral tend to light alkaline and ranged between (7.34 - 8.10), the electrical conductivity ranged between (7021 7021 - 7947 7947) µs/cm. while the TDS, ranged between (09.502 09.502 - 729950. 729950.) mg/l, according to the values of salinity ranged between (0.773 - 0.954)‰ the water of the river was classified as Oligohaline, while the values of Light Penetration ranged between (28.81 - 120.61)cm, and the turbidity ranged between (43.26 - 55.78) NTU, also showed dissolved oxygen as its value ranged between (45729 45729 - 7.979) mg/l, while the biological oxygen demand showed value ranged between (1.123 - 2.627) mg/l, The values of total organic carbon in sediments ranged between (0.440 - 1.045)%, While the sediment texture was sandy - silt in the first site, and sandy - clay in the both two and three sites of Al - Diwaniya river. Results of Concentration of trace elements (Cadmium, Copper, Nickel, Lead and Zinc) in dissolved part ranged between (252222. 252222. - 2522724 2522724), ( 252270 252270 - 252200 252200), ( 25227. 25227. - 252290 252290), (252224. 252224. - 2522022 2522022 ) , ( 25279. 25279. - 252010 252010 ) mg/l., whereas in particulate part ranged for between (0.29 - 1.49), (3.46 - 13.04), (12.51 - 20.99), (1.08 - 4.67 ((, (65.54 - 127.51 ) µg/g dry weight. and in sediments ranged between (0.53 - 1.96), (23.93 - 49.65), (136.85 - 166.09), (4.14 - 5.92), (126.69 - 176.64) µg/g dry weight respectively. In the Descending sequence in concentrations of all trace elements in abiotic components was as follow : sediments> particulate part> dissolved part. in producers of Al - Diwaniya river ranged in plant P. australis from (0.07 - 0.20), (5.93 - 16.21), (8.67 - 46.87 ) , (0.89 - 2.93), (26.60 - 103.78) µg/g dry weight respectively. in phytoplankton from (0.40 - 1.77), (8.89 - 25.2), (28.0666.49), (1.47 - 3.59 ) , (103.39 - 159.70) µg/g dry weight respectively. In the Descending sequence in concentrations of all trace elements in producers was as follow phytoplankton> plant P. australis. While the concentrations of trace elements in consumers in (zooplankton) of Al - Diwaniya river ranged from(0.56 - 4.41), (21.89 - 56.08), (51.64 - 152.79), (2.806.06), (137.14 - 183.61) µg/g dry weight respectively. While in two species of fish according to organs in gills of Cyprinus carpio ranged from(0.63 - 2.07) ) ) 3.19 3.90), ) ) 4.04 - 8.96) , (1.95 - 3.76), (182.14 253.89) µg/g dry weight respectively, in liver from(0.41 - 1.78), (6.20 - 32.47 ) , (5.97 - 10.39), (2.06 - 5.37), (136.15 184.75) µg/g dry weight respectively, and in muscles from (0.49 - 3.43و) , (و 2.82 10.40), (7.17 - 11.57 ) , (0.61 - 4.60 ) , (110.96 - 160.46) µg/g dry weight respectively. and ranged in gills of Tilapia zilli from(0.44 - 1.15), (1.06 - 6.83), (8.18 - 13.97 ) , (1.46 2.89) , (99.03 155.33) µg/g dry weight respectively, in liver from(0.571.64 ) , (13.08 - 28.06), ( 8.03 - 18.50), (1.32 - 3.02), (112.68 176.32) µg/g dry weight respectively, and in muscles(0.46 - 1.80), (2.42 2.93), (7.47 - 10.52), ( 0.20 - 3.16 ) , (79.74 - 153.58 ((µg/g dry weight respectively. concentrations of all trace elements in consumers always were higher than with producers and abiotic components In the Descending sequence in concentrations of all trace elements in consumers and producers was as follow : zooplankton> Cyprinus carpio> Tilapia zilli> phytoplankton> plant P. australis., And in the organs of fish was as follow in Cyprinus carpio : gills> liver>muscle, while in Tilapia zilli was follow liver > gills >muscle. While the values of the bioconcentration factor for (Cadmium, Copper, Nickel, Lead and Zinc) in producers of the study area ranged in plant P. australis. From 747542 747542)) - 7200500 7200500), ( 0.7150. 0.7150. - 729445.4 729445.4), (..1750...1750. - 047..59. 047..59.), ( 004540 004540 411507 411507), ( 44.592 44.592 - 4740509 4740509) respectively.In phytoplankton (7027512 7027512 - 000.2522 000.2522), (.704504.704504 - 79...549 79...549), ( 7111.547 7111.547 - 99420594 99420594), ( 7049542 7049542 - .20454..20454.), ( 0110527 0110527 77944502 77944502) respectively. the values of the bioconcentration factor for elements in consumers of the study area ranged in zooplankton from (712.59. 712.59. - .7444544.7444544), (0071504 0071504 - 42914504 42914504 ) , ( 0.004597 0.004597 - 07.700524 07.700524), ( 0..7527 0..7527 - 7709.544 7709.544), ( 4010577 4010577 70744574 70744574) respectively.in fish organs Cyprinus carpio ranged in gills from(197571 197571 - 97922522 97922522), (839.47 - 2808.33), (1704.16 - 4640.00), (1500.00 - 4076.92), (6876.10 - 13873.77) respectively. in liver ranged from (482.35 - 35600.00), (001.522 001.522 - 10075.00), (2473.81 - 6453.33), (1665.00 - 4646.15), (6373.16 - 12568.02) respectively. and in muscles (.14591.14591 - 40422522 40422522), ( 190572 190572 - 0444544 0444544), ( 7049500 7049500 9002500 9002500 - ) , ( 047520 047520 - 1214540 1214540), ( 4220501 4220501 - 9111502 9111502) respectively. while in Tilapia zilli organs ranged in gills (.71549.71549 - 00222522 00222522), ( 000500 000500 - 01015.2 01015.2) , ( 000254. 000254. 4070500 4070500), ( ((00475.0 00475.0 - 40.504 40.504 , (.40050..40050. - 0901541 0901541 ) respectively. in liver (4125.0 4125.000022522 00022522 ) , (.02.504.02.504 - 00000500 00000500), ( 0017590 0017590 - 77.405.2 77.405.2), ( 09457. 09457. - 9044500 9044500) , (499.5.0 499.5.0 - 0420592 0420592 ) respectively. and in muscles ranged from (.97571.97571 - 00022522 00022522), (771.05 - 0744544 0744544) , ( 7414574 7414574 - 401.522 401.522), ( ( ( 0.44500 0.44500 - 700502 700502, ( 5414.28 - 7354.09) respectively. results showed that the aquatic organisms (Consumers, Producers) have ability to bioconcentration of trace elements. and showed the descending sequence in concentrations of all trace elements in consumers and producers was as follow : zooplankton> Cyprinus carpio> Tilapia zilli> phytoplankton> plant P. australis. The result of this study confirms the biomagnification phenomenon (the increase of trace elements concentration with elevation in trophic levels of the food web) in study of Al - Diwaniya river ecosystem, as it's the case in other region of the world

دراسة تشريحية لسيقان وسويقات واوراق بعض اجناس العائلة الوردية rosaceae في الديوانية == Anatomical Study of The Stems, Leaves And Petioles For Some Species of Family Rosaceae

اسم المؤلف: حسنى مهدي رياح الفتلي
اسم المشرف: ازهار عبد الامير سوسة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شمل البحث الحالي دراسة بعض الانواع العائدة للعائلة الوردية Rosaceae في محافظة الديوانية من الناحية التشريحية, اذ تم دراسة المقاطع المستعرضة للسيقان والسويقات والاوراق لثلاثــــــــــــة انــــواع وهـي المشمـــــش Prunus armenicana L. والكمثـــــــــري.Pyr | Current research involved the study of some species belonging to the family rosary rosaceae in Diwaniyah province of anatomical terms, as has been the study of cross sections of the stems, leaves and petioles for three species apricot Prunus. armenicana L. And pear Pyrus communis L. And apple Malus domestica Borkh. The study has been the separation of these species into groups depending on differences and similarities of the attributes of the obvious anatomical changes were discussed among these species, It turns out that many of these qualities, Especially the form of stem and cutworms as well as the presence or loss of tissue such as textiles Parencyma green Chlorenchyma in the stem as possible discrimination species P.armenicana for the other two species of loss of such a fabric, And also the possible discrimination of this species through the general shape of the clips transverse Soik as it's looks like a horse shoe, While the other two it's like a triangle. As for the indumentum and taxonomic diversity epithet to isolate and diagnose these species from each other, Such as Glandular hairs and the loss of these filaments in the other two species.The study concluded that the possibility of the use of the anatomical characteristics of transverse sections of the Stem, Petioles and Leaves in the diagnosis of the species under study

استجابة نبات السلق (Beta vulgaris var. cilca L.) للشد الملحي وحامضي البرولين والجبرلين == Response of Chard Plant (Beta Vulgaris Var. Cilca L.) To Salt Stress, Proline And Gibberellic Acids

اسم المؤلف: حيدر عبد الامير مظهور
اسم المشرف: انتصار حسين مهدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: نفذت التجربة في الموسم الشتوي للعام (2012 - 2013) م في احد الحقول التابعة لناحية الحر في محافظة كربلاء, لمعرفة استجابة نبات السلق للشد الملحي والرش بحامضي البرولين والجبرلين والتداخل بينهما في بعض الصفات الكمية والنوعية لبعض الاجزاء الخضرية والتكاثرية (ا | An experiment was conducted in the winter season of the year (2012 - 2013) in a private Al - Hur district in Karbala governorate, to find out chard plant response to salt stress, proline and gibberellic acids and their interaction on some quantity and quality characteristics to some parts of vegetative and reproductive (leaves, flowers and seeds) to chard plant (Beta vulgaris var. cilca L.).The design of the experiment was randomized complete blocks (RCBD) in a factorial arrangement with three replicate. The first factor was four levels of saline water (0(River water), 2, 4 and 6) ds. m - 1 and the second factor was seven concentration of proline acid (PA) and gibberellic acid (GA3) (0(River water), 50PA, 100PA, 150PA, 50GA3, 100GA3 and 150GA3) mg. L - 1. Means were compared by using averages revised least significant difference (RLSD) at 0.05 probability level when the treatments referred to significant effect. The results showed : 1 - The negative effect of salinity levels on the qualities of the vegetative plant chard before flowering stage with the except of the dry weight of the root system, which had increased significantly with the level of salinity 2 ds. m - 1.2 - The negative impact of salinity levels in vegetative qualities of chard plant after flowering except root length, leaves chlorophyll content (a, b and total), nitrogen%, protein% and proline which increased with the level of salinity 2 ds. m - 1. as well as the potassium% in leaves with a salinity level 4 ds. m - 1 and dry weight of shoots with all levels of salinity and especially 6 ds. m - 1.3 - Gibberellic acid was dominant on proline acid in terms of chard plant by 150 mg. L - 1 concentration before flowering and 50 mg. L - 1. concentration after flowering while proline acid was dominant on gibberellic acid in dry weight before flowering and root dry weight of shoots after flowering, by 50 mg. L - 1 and potassium% in leaves and the protein% with 100 mg. L - 1 concentration.4 - The interaction between the salinity levels and acids concentrations add showed a significant difference in most of the traits of the plant, especially in combinations consisting of level 2 ds. m - 1 or comparison of saline with gibberellic acid by 150 mg. L - 1 before flowering and 50 or 100 mg. L - 1 after flowering while gave a combination of salinity level 4 ds. m - 1 with concentration of proline acid 100 mg. L - 1 higher of potassium% in leaves

تاثير المعاملة ببعض مبيدات الافات والمواد الكيمياوية في نمو الفطرين Alternaria tenuissima وFusarium oxysporum المرافقة لبذور وجذور الحنطة Triticum aestiumn == Effect of Some Pesticides And Chemicals In The Growth of Fungi Alternaria Tenuissima And Fusarium Oxysporum Associated With The Seeds And Roots of Wheat (Triticum Aestiumn)

اسم المؤلف: مهند جاسم حمود
اسم المشرف: عبد الامير سمير سعدون
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اذ تم في هذه الدراسة عزل عدة انواع من الفطريات المرافقة لجذور وبذور الحنطة تموز 2 مصدق 1وتم تشخيص تسعة انواع من الفطريات المرافقة للبذور وبنسب تردد مختلفة وهذه الانواع هي Aspergillus niger, Penicillium notatum , Alternaria tenuissima, Rhizopus stotanifer | As it was in this study to isolate several types of fungi associated with the roots and seeds of wheat July 2 certified 1 was diagnosed nine types of fungi associated with seeds and different frequency rates and these types areAspergillus niger, Penicillium notatum, Alternaria tenuissima, Rhizopus stotanifer P. digitatum, Fusarium oxysporum, A.flavus, Fusarium solani, A. alternata. Was diagnosed nine types of fungi isolated from the roots of wheat and the surrounding soil and different frequency rates and these types. A. alternata, A. niger, R. stolanifer, Mucor sp., Trichoderma hazianum, P. notatum, F. oxysporum, F. solani, Alternaria tenuissima. Testing was diagnosed two fungal species tested using a technique monomer chain reaction PCR. These species are fungal Fusarium oxysporum, Alternaria tenuissima. This study tested the effect of pesticides is a fungal pesticide jungles Granstar and insecticide Wide. The transactions chemical which urea and NaCl in the growth of some fungi isolated from the seeds and roots of wheat as it has been test the effect of different treatments and their interaction in the germination of seeds of wheat on the filter paper and compare nets comparison innate Elsa (Carbendazim) and underwent tests the effects of different treatments and their interaction in radial growth and dry weight of the two types of fungi isolated in the germination of their spores and bacterial length of the tube, as well as test the effect of transactions and their interaction in the germination of seeds in the soil sterile and non - sterile. The results showed that the use of urea and sodium chloride concentration of 15 mg / mL in the treatment of seeds of wheat led to increased germination of these seeds and the impact of a negative in some types of pathogenic fungi associated with them as well as the use of pesticides other than fungal pesticide jungles Granstar and insecticide Wide negative effect also in the vitality Activity and fungi.germination The results showed the existence of significant effects on rates of seeds treated with pesticides and salts on the filter paper analogy transactions comparison since given concentration 15 mg / 10 mL of pesticides and 15 mg / ml of salt are high in the germination of the seed treatment, which exceeded ratios germination of seeds of wheat treatment urea fertilizer treatment fungicide Elsa. As rate ratios germination of wheat seed treatment with urea 96.66%, while the rate ratios germination of seeds of wheat pesticide Elsa 93.33%, followed by treatment of pesticide Wide as rate ratios germination 83.33% and 80.00% for wheat seed when the focus 15 mg / ml of sodium chloride salt treatment, and after treatment Karan Star as the rate of germination rates of seeds of wheat 73.33%. The results showed the existence of significant effects in increasing the proportion of seed germination treatment with pesticides and salts in the soil sterile and non - sterile as it gave the Focus 15 mg / 10 mL of pesticides and 15 mg / ml of salts highest rates of germination of these seeds came these results are compatible with the results of seed germination in the filter paper. The results show that as well as the individual transactions were more influential than the transactions overlap in radial growth and dry weight of fungi testers and their spores germination rates and lengths of germ tubes, as growth rates of radiographic Fungi F. oxysporum and A. tenuissima in the treatment of a mixture Wide and Granstar 11.44, 8.55 mm, respectively, while the rates of diameters of the colonies for the treatment of urea pesticide Wide 33.66 and 26.66 mm, while the pesticide mixture Wide treatment with sodium chloride amounted to 37.00 and 15.00 mm, in while rates were diameters of colonies for the treatment of a mixture Granstar with sodium chloride, 52.66 and 25.00 mm, and the growth rates were radiography for the treatment of pesticide Granstar with urea 25.00 and 11.66 mm, and the growth rates for the treatment of sodium chloride with urea 31.24 and 37.33 mm, compared with the fungicide Elsa, which amounted to the radial growth rates of 8.55 - 11.44 mm Fungi testers respectively.

تاثير الاجهاد المائي والسماد الورقي والمخصب العضوي في نمو نبات حلق السبع الشجيري Adhatoda vasica (L.) Nees ومحتواه من بعض المواد الفعالة وفعاليتها المضادة في بعض بكتريا تسوس الاسنان == Effect of Water Stress, Foliar And Organic Fertilizers On Growth of Adhatoda Vasica) L.(Nees. And Its Content of Some Active Ingredients And Inhibition Activity of Some Dental Caries Bacteria

اسم المؤلف: ليث سريع الركابي
اسم المشرف: ثامر خضير مرزة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: This study was conducted in Biology Department - College of Science - University of Al - Qadisiya that included pot experiment from the period of 1/4 to 1/8/2013, to assess the effect of water stress, foliar fertilizer (Foltron) and organic fertilizer (Azomin) on growth of Adhatoda vasica (L.) Nees and Its Content of Some Active Ingredients. Laboratory experiments were also done from the period of 1/8 to 1/3/2014, to determine the activity of leaves and roots extracts that resulted from plants of pots treatments to inhibit growth of some bacteria genera causing Dental Caries i.e. Staphylococcus aureus, Streptococcus mutans and Streptococcus pneumonia.A pot experiment included seventy two transplants (age of one month and 8 cm height) that were transplanted in plastic pots (one transplant per pot) in 1/4/2013. Pots were filled with sandy soil and peatmose 2 : 1 v/v. Plants Irrigated with two levels 50 and 25% of field capacity. Foliar fertilizer was used at three concentrations of 0, 3 and 6 ml/L that sprayed twice on plant shoot till complete wetness during 15/4 and 1/6/2014. Organic fertilizer was also used with irrigation water at three concentrations of 0, 4 and 8 ml/L twice on 1/5 and 15/6/2014. The design of the experiment was Randomized Complete Blocks Design (RCBD) in a factorial arrangement (2×3×3) with four replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.05 level when treatment effect was significant.Results of pots experiment were as follows : 1 - Increasing water stress level caused a significant decrease in the most characteristics of vegetative and root growth except for stem internode and root lengths, while increasing in concentration of foliar and organic fertilizer gave the opposite effect of water stress.2 - Water stress caused a significant decrease in leaves content of total chlorophyll and nutrient elements N, P, K, Mg and protein percentage, while foliar and organic fertilizer caused a significant increase on the above characteristics.3 - Water stress caused a significant increase in proline percentage and Vit.C. and Vasicine and Vasicinone alkaloids, while foliar and organic fertilizer caused a significant decrease in proline percentage and Vit.C. and a significant increase in Vasicine and Vasicinone alkaloids.4 - The treatment of plants that were subjected to the water stress via foliar or root fertilizing or both of them caused a significant increase for most of vegetative and root growth characteristics, total chlorophyll, nutrient elements, protein percentage and Vasicine and Vasicinone alkaloids.5 - The treatment of plants that were underwent to the water stress via foliar and root fertilizing caused a significant decrease in proline percentage, Vit.C. and stem internode and root lengths.6 - The interaction of the three studied factors gave a significant positive effect to rise alkaloids of plant.Laboratory experiments included picking fresh leaves and root of plants that dried aerial in a shade, and then extracted it via methanol alcoholic of 70%, it was detected for some chemical components of extract by using reagents. Bacterial genera were isolated from the surfaces of the teeth and cultured on the growth media, and it was diagnosed by the Vitek Compact 2 System Diagnosis. The activity test involved the impact of the alcohol extract for leaves and roots to the inhibition of bacterial growth by the agar well diffusion method on Muller - Hinton.The design of the experiment was Complete Randomized Design (CRD) in a factorial arrangement (18×3) with three replications per each treatment. Revised Least Significant Difference (RLSD) was used to compare treatment means at probability 0.01 level when treatment effect was significant.Results of laboratory experiment were as follow : 1 - Increasing extracts concentration caused a significant increase in growth inhibition of the studied bacterial genera.2 - plants extraction that of the combination of A2B3C3 included (25% water stress, 6 ml/L foliar fertilizer and 8 ml/L organic fertilizer) gave the highest activated inhibition for bacterial genera compared with plant other extraction for all tested bacterial genera.3 - The plants extracted that treated by combination A2B3C3 in concentration 150 mg/ml gave higher activated inhibition for Streptococcus pneumonia reached 25.307 ml compared with Amoxicillin that reached 36.167 ml, while the inhibited extracted root reached for the same treatment 16.060 ml (Table 37 and 38).4 - Plants leaf extract was the highest effective inhibition of bacterial compared to the roots extract.

دراسة بيئية للطحالب الملتصقة على بعض النباتات المائية في مياه مبزل الفرات الشرقي (الحفار) / الديوانية - العراق == An Ecological Study of Epiphytic Algae On Some Aquatic Plants In East Euphrates Drainage Water /(Al - Haffar) Al - Diwaniya/Iraq

اسم المؤلف: زهرة كليب مهدي الخزعلي
اسم المشرف: فؤاد منحر علكم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الاحياء المائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناولت الدراسة الحالية الخصائص البيئية المؤثرة على الطحالب الملتصقة على النباتات المائية في مبزل الفرات الشرقي (الحفار) في الديوانية - العراق, اذ شملت الدراسة قياس بعض الخصائص الفيزيائية والكيميائية لمياه المبزل والخصائص الاحيائية التي تضمنت دراسة كمية ون | The present study tackles ecological characteristics which is effected of Epiphytic algae in east Euphrates drainage (AL - Haffar) in AL - Diwaniya, This study included the measurement some of physical and chemical characteristics of drainage water, and biological characteristics which includes studying quality and quantity of Epiphytic, Three aquatic plants Ceratophyllum demersum, Phragmits australis, and Imperata cylindrical had been selected, Samples collected monthly during The period from November 2012 to October 2013.Three location have been chosen, the first is near AL - Abtan village, Second is after the first location five kilometers, while the third location is after the Second about seven kilometers. The study shows that the temperature of the air is about (7 - 39c) and water temperature is approximately (10 - 33c), while light penetration is about (10.5 - 63cm) the flow speed is (0.25 - 0.68 M/Sec), Water turbidity is (40.02 - 63.03 NTU), while Electrical conductivity is approximately about (3014 - 3964 Sc/cm). The results of the study shows that the water drainage Oligohaline, the salinity values ranged from (1.92 - 2.53‰), the total (T.D.S) is about (1741 - 2226 mg/l), while the T.S.S. is about (41 - 67 mg/l). The result of this study shows that the pH is limited Through all the period of the study (6.98 - 8.18), The drainage water get good airing dissolved oxygen values are(6.71 - 8.14 mg/l), biological oxygen demand are (2.05 - 5.23mg/l) and carbon dioxide values are about (81.50 - 119.57mg/l).The results of this study also show about that drain water is alkaline and belonged to bicarbonate ions, total alkaline is (140.28 - (189.27 mg/l) It is discovered that the drain water is too hard, hardness values are (512.15 - 612.45mg/l), while Calcium concentrations are (128.36 - 173.40mg/l) and Magnesium concentrations are (30.31 - 50.09 mg/l). Plant nutrients, N concentrations are about (0. 98 - 3.99 µg/l) of Nitrit, Nitrate concentrations are (216 - 415 µg/l) and Phosphate concentrations are about (0.9 - 2.92 µg/l), and The percentage of total organic carbon in the residues is(0.59 - 0. 17) %. The total number of epiphytic algae types which are diagnosed during study period reaches to 320 species belongs to 86 genera within 7 classes They are Bacillariophyceae, Chlorophyceae, Cyanophyceae, Euglenophyceae Xanthophyceae, Dinophycea eand Rhodophyceae, Bacillariophyceae obtaints the dominion which reach to (183) species of Bacillariophyceae. It makes rate (57.19%).Than Chlorophyceae came concerning types number 68 species in rate (21.25%) , Following by Cyanophyceae 53 species in rate 16.56%. Euglenophyceae are 10 species in rate (3.13%).Xanthophyceae is represented in two species belong to2 genera.It makes proportion (0.63%) , Dinophyceae which represented in 3 species belonged to 3 genera in rate (0.94 %) and Rhodophyceae represented in one species and one genera in rate (0. 3%) of total number of algae.Class of the Bacillariophyceae, during study period were Navicula وNitzschia وCymbella وSynedra وAchnanthes, genera of Oscillatoria and genera of, Scenedesmus manifest that highest number of available types during the period of study.It has note that there are numbers of the monthly and located changes in total number of epiphytic algae on host plants.was varied in months and locations due to the host plants. Ceratophyllum demersum marks the Highest total number of epiphytic algae 172.89× 104 individual/ gm of wet weight in third location during April. Imperata cylindrica marks the lowest total number of epiphytic algae 3.51×104 individual/ gm of wet weight in scecond location during January, The total number of epiphytic on host plants ranges between (175.39 - 668.6) ×104 individual/ gm of wet weigh.The total number of main epiphytic algae classes is varied in total number of epiphytic algae.Bacillariophyceae diatoms composed 81.02%, in all locations during the study period, followed by Cyanophyceae composed 9.7%, while Chlorophyceae composed 8.6%.Euglenophyceae, Chrysophyceae, Dinophyceae and Rhodophyceae, were not important concerning their number. Statistical analysis results show there are significant differences between locations and months of study in relation to physical, chemical and biological features. Also there are connected correlations positive and negative between epiphytic algae and physical, chemical and biological features that measured during the study period of probability level of P<0.05.

دراسة تشريحية لبشرة اوراق بعض اجناس العائلة المركبة Compositae (Asteraceae) في جامعة القادسية == Anatomical Study of The Leaves Some Genus Compositae (Asteraceae) In Al - Qadisiyah University

اسم المؤلف: فرقد حيدر عبد النبي المحنة
اسم المشرف: ازهار عبد الامير سوسة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تشريحية مقارنة لصفات البشرة للاوراق بعض الاجناس التابعة للعائلة المركبة (Compositae) وهذه الاجناس هي Aster L. و.Calendula L وDendranthema L. و.Helianthus L و.Lactuca L و.Launaea L و.Tagetes L, وتبين ان لبعض الصفات التشريحية اهمية | The present research includes comparative anatomical study epidermis of some genera which belong to sun flower family (Compositae) These genera are Aster L., Calendula L., Dendranthema L., Helianthus L., Lactuca L., Launaea L. and Tagetes L. , and show that details anatomical the importance of taxonomic possible from isolation genuses more each adjective undulate the walls vertical epidermis the leaves, and we show some importand for indumentum suchas T - shape eglandular haire in Dendranthema L. species

دراسة جرثومتي السالمونيلا والشيكلا المعزولة من حالات سريرية في مدينة الديوانية وتاثيرهما على معايير دم المرضى

اسم المؤلف: هبة اركان فنطيل الحسناوي
اسم المشرف: علي عبد رحيم الناشي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: Salmonella is a bacteria and Shigella types of intestinal bacteria, and because of the seriousness of the pathogenicity and the spread of infection in their environment has been conducting this study.Collected during the current study, 462 different clinical sample (feces and urine) of adults aged 20 years and older and those with symptoms of the bacteria Salmonella and Shigella and both sexes reviewers for laboratories and hospitals in the city of Diwaniya, for the period from 2012 \ 11 \ 6 to 2013 \ 5 \ 26. Diagnosed isolates based on phenotypic traits and AGRO tests biochemical in addition to diagnosis by Api20E system as isolates Salmonella reached 17 isolation and by 3.6% and were all belonging to the type S.typhi, either isolates bacteria Shigella was three isolates and 0.64%, and all of them belong to the type of Sh. Flexneri. The incidence of bacteria S.typhi for the male sex was higher than in females, Ozbulg number of infected males and 10 by 58.8%, while the number Fayalnads was infected 7 and by 41.1%, but for bacteria to Sh. flexneri, as was the incidence in males is also higher than in females as in males reached 2 and by 66.6%, while the number of infected females in bringing only one injury and 33.3%. Were more age groups injury is a class 20 - 29 where recorded 9 injuries and by 52.9% and the number of infected males 6 The number of cases in females 3 injuries, age group 30 - 39 recorded 5 injuries and by 29.4 and were two goals in the male and three injuries in females, the age category 40 - 49sgelt two goals by 11.9% and the injury was confined to males either category above 50 years and one injury recorded only in females and 5.8%, either in bacteria Sh. flexneri was the only injury in the 20 - 29 age group and by three injuries. The more isolated the proportion of bacteria S.typhi in the month of May and April as recorded 4 isolates each, and by 23.5%, while giving the lowest ratio for the presence of bacterial during the months of December and January, as recorded isolation and one each of these two months and 5.8% either November remaining months February, March was the number of isolates in 3, 2, 2, respectively, and 15%, 17.6%, 11.7, 11.7%, respectively, as well, either for the bacteria Sh. flexneri as Bulgtaaly proportion of injury in the month of May was the number of isolates 2 and by 66.6% during the month of April either recorded a hit single Vqtobnsph 33.3% did not notice any injury to the other months of the study. Tested the sensitivity of the isolates S.typhi and Sh.flexneri direction antibiotics used as 8 used antibiotics in common use in this study show that the bacteria were sensitive S.typhi 100% Anti Ciprofloxacin, but resisted antibiotics Ampicillin, Amoxicillin, Chloramphenicol, Tetracycline, Nalidixicacid, Gentamicin, Ceftriaxone at a rate of 82.3%, 82.3%, 76.4%, 70.5%, 64.7%, 23.5%, 23.5% respectively. The bacteria Sh.flexneri was 100% sensitive to antibiotics Ciprofloxacin, Gentamicin, Nalidixic acid, Amoxicillin, Ceftriaxone while it was resistant to antibiotics Tetracycline, Chloramphenicol, Ampicillin by 66.6% each. Been investigating some of the virulence of the bacteria S.typhi factors, including the presence of the portfolio Capsule as were all Salmonella isolates characterized by possessing the portfolio, while the production of the enzyme protease Protase was all isolates S.typhi producing this enzyme also showed 12 isolation of the ability to produce the enzyme Lipase and increased by 70.5%, but all isolates were S.typhi unproductive enzyme protease. The bacteria Sh.flexneri marked by Azltan of three Azladtha and by (% 66.6) viability link red dye Congo also showed 2 of these isolates and by (66.6) ability to form thin membrane (Pellicle) and two Aezzltan which have demonstrated susceptibility to adhesion was isolated one of them only possess cilia first pattern type 1. The research covered also study the changes that occur in some of the blood parameters in people infected with the bacterium Salmonella Heclauzar that there is a decrease in the total number of red blood cells Red blood cells in people infected with Salmonella, where the total number of blood cells Reds reached 4.91 x 106 c \ ml total number of cells rate 3.7 white blood x 103c \ ml the proportion of types of white blood cells of the total number of cells, the white was lymphocyte ratio lymphocytes% 52.55 which is low compared with the normal number, while there was an increase in both acidophilus cells Eosinophils and only Monocyte as she was represented 1.2%, 2.9% in November while it was% 0.03 Basophils cells, the highest percentage of increase in healthy people, and the amount of total hemoglobin rate. Hemoglobin9.07 g \ dl which is low compared with healthy peopleBut in people infected with bacteria Sh. Flexner, bringing the average number of red blood cells 106 c \ ml3.7 x, the lowest rate with the number of healthy people, as the amount of hemoglobin g \ dl13.5 rate, either the total number of white blood cells, the rate was slightly higher than what is found in healthy people Azbulg (11.32 x 103c \ ml), it was noted that most of the increase was high in the white blood cells of the type Neutrophils the increase amounted to 62.34%

تطبيق بعض طرق حفظ الحشرات على حشرة المن == Applicating of Some Methods of Maintainance On Aphids

اسم المؤلف: ضياء عباس عبد الرضا الزيادي
اسم المشرف: عبد المحسن حسين مؤنس
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تعد الحشرات ذات اهمية اقتصادية وطبية وجمالية مما دعت بالباحثين الى دراستها من كافة الجوانب الفسلجية واطوار حياتها وتكيفاتها ومناطق استيطانها وطرق تغذيتها للوصول الى حلول كفيلة للحد من الاضرار المسببة عن الحشرات الاقتصادية والطبية من جانب ولتكثير وتربية ال

دراسة انتشار انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا == Studying The Spreading of Fungus Saprolegnia SPP. In Waters of Diwaniyah City And Characterization The Molecularly

اسم المؤلف: معتز محمد عزوز الزاملي
اسم المشرف: ماجد كاظم عبود الشبلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة الحالية عزل وتشخيص بعض الفطريات المائية ومنها انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا. عزلت الانواع التابعة لهذا الفطر وهما Saprolegnia parasitica وSaprolegnia ferax من الانهار والبحيرات والاسماك, وتم قياس الخصائ | The present study is concerned with isolation and identification of some aquatic fungi, including species of Saprolegnia spp. in waters of Al - Diwaniyah city and molecular characterization. Two species, Saprolegnia parasitica and Saprolegnia ferax, have been isolated from rivers, lakes and fishes. The physical and chemical environmental characteristics have been measured. The types above have been examined to extract the DNA. The (PCR) technique has been used to examine the presence of protease enzyme. The results of isolation and identification showed that the percentage of the two species were 64% in rivers areas 40% in the lakes areas and 50% in fishes. The rates represented the examined samples which were 60 samples. AS well as isolates was in rivers 16 isolation and in Lakes 10 isolates either in fish was 5 isolation and thus the total isolates 31 isolation, The S. parasitica species is more apparently found during (February, 2014), the number of samples was 12, the less presence of this species in (April, 2014), where the number of sample, was 3. The S. ferax species is more apparently presented in (February, 2014), the number of samples was 10. The less presence was in (April, 2014), the number of samples was 2. The results showed that the number of isolation of Saprolegnia spp. study area as it was S.parasitica species in were 6 in rivers, 2 in lakes, and 3 in fishes. The second species, S. ferax showed number of isolations which were 10 in rivers, 8 in lakes, and 2 in fishes. The PCR examination has used to detect the rDNA gene which responsible for proteases gene and whether it is found in the two species of fungi. The express way has been used to extract DNA and according to the instructions of manufacturer company. The concentrations of DNA in first species were 121.8 - 73.0 mcg/microliter and purity 1.87 - 1.80.In the second species the concentrations of DNA were 2360.4 - 166.9 mcg/microliter and purity 2 - 1.75. The results, by using PCR technique, showed that the percentage of protease in first species was 25% in rivers. In lakes and fish was 100%.Concerning the second species, the percentage of enzyme was 42% in rivers, 40%in lakes, and 50% in fishes. It can be concluded that there is a diversity in fungi species with respect to study areas and enzyme secretion in the stages of fungi infection. The enzyme is more apparently found in S.parasitica species compared with S.ferax species.

دراسة مصلية دمية لداء المقوسات في النساء الحوامل والاطفال حديثي الولادة في محافظة القادسية == Bloody Serological Study of Toxoplasmosis In Pregnant Women And Newborns In Diwaniyah Province

اسم المؤلف: مروة جبار مزهر العبادي
اسم المشرف: خيري عبد الله داود العكيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على الاطفال حديثي الولادة في محافظة الديوانية للمدة من بداية شهر تشرين الثاني 2012 الى نهاية شهر نيسان 2013.وقد جمعت عينات الدراسة من دم الحبل السري للاطفال الرضع المولودين حديثا اثناء الولادة في مستشفى الاطفال والولادة التعليمي في | The present study was done on new born babies in Diwania province during a period from the beginning of November 2012 till the end of April 2013. Blood samples were collected from the umbilical cord of the new born babies during delivery in children and maternity teaching hospital. Fifty samples were collected separated to serological tests and for blood pictures. Serological tests were used in this research as follows (Rapid IgG - IgM test, latex test and ELISA IgG, IgM). The results showed the Rapid test was the easiest and cheapest test for diagnosis specific antibodies against Toxoplasmosis, therefore the positive samples were 14% for IgG, 4% for IgM and 36% for overlap cases. While the positive results for latex test was 14% but the highest result was recorded by using ELISA test 30% for IgG, No positive result recorded for IgM. There were variation in positive cases of different ages of mothers, the highest percents found in ages between 30 - 39 years old by using Rapid test 72.72% , while by using The latex test demonstrate high percent of infection was 20% and also for ages between 15 - 19 year old, and the ELISA recorded 50% and the same group of age 15 - 19 years old. The results showed the for the residence factor by using Rapid test showed the rural people were high percent of incidence of Toxoplasmosis 57.14%, but the urban people recorded Latex test and ELISA)17.24%, 31.03%) respectively. Aborted women in previous time were followed by using the serological tests Rapid test 10%, Latex 10% and ELISA zero. The incidence of Toxoplasmosis was high in housewives more than other career, the results were) 34.14%, 34.14 (% for Latex and ELISA, IgM and overlapping cases, while in other career by using Rapid test 66.66% Results showed standard Blood parameters did not register significant differences (P> 0.05) for the total number of white blood cells WBC, hemoglobin Hb, blood volume compact PCV, Erythrocytic RBC, and differential count of white blood cells for Neutrophiles, Lymphocyte, Monocytes, Basophiles and Eosinophiles. Phagocytic activity was prominent in adult people more than newly born babies.

تاثير الثايموكوينون الخافض للسكر والمجدد الانسجة بنكرياس الجرذان المستحث فيها داء السكري باستخدام الستربتوزوتوسين == Antihyperglycemic And Pancreatic Regenerative Effect of Thymoquinone In Streptozotocin Induced Diabetic Male Rats

اسم المؤلف: وجدان ثامر مهدي التميمي
اسم المشرف: جبار عباس احمد الساعدي | هاشم محمد عبد الكريم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: To evaluate the anti - hyperglycaemic potent of thymoquinone from Nigella sativa seed in streptozotocin - induced diabetic male rats, the present study has been carried out at the College of Education, Al - Qadisiya University during the period extended from April, 15, 2012 to December, 15, 2012. mRNA expression level of Reg3a, InsI, InsII, PDX1, Pax6, NeuroD1, and MafA genes have been evaluated in pancreatic tissues as well. Sixty five adult male rats (aged 56 days and weighted 138±8.8g) have been used in the present study. Diabetes has been inducted in 52 male rats by injection of single dose of streptozotocin (60 mg/ kg, b.w., i.p.). Diabetes mellitus has been confirmed by blood glucose concentration (to be more than 200 mg/ dl). Intact and streptozotocin - induced diabetic male rats have been assigned to five equal groups (13 per each); Intact (C) and non treated diabetic (DM) rats have been injected with normal saline (100?l, sc) anddrenched with drinking water daily for 42 days. Thymoquinone treated diabetic rats (TQ50 and TQ100) have been injected with normal saline (100?l, sc) and drenched with thymoquinone suspention (50 and 100 mg/ kg, b.w., respectively) daily for 42 days. Insulin treated diabetic rats were injected with insulin (4 IU, sc) and drenched with drinking water daily for 42 days. Body weights were registered daily during the experiment. All overnight fasted animals were sacrificed after general anesthesia by combination of xylazine and ketamine (10 mg and 90 mg/kg, ip, respectively). Blood samples was collected from abdominal vein for determination of serum glucose and insulin concentrations. Samples from pancreatic tissues in all groups have been quickly removed, dipped in liquid nitrogen for RNA extraction and molecular study. Other pancreatic tissues were fixed in formalin forhistopathological and immunohistochemical study. The results demonstrated significant decrease in body weight gain of untreated diabetic (DM) and insulin treated diabetic (DMI) groups as compared with that of intact control (C) and thymoquinone treated diabetic (TQ50 and TQ100) groups, started from the fourth day of experiment, which showed insignificant differences when compared with each other. While the lowest body weight gain has been registered in DM group.Results of serum glucose concentrations referred to significant elevation in diabetic groups compared with intact control. In comparison between the diabetic groups, glucose concentration revealed significant decrease in thymoquinone and insulin treated rats (TQ50, TQ100, and DMI) compared with untreated diabetic rats (DMI). on the other hand, insulin treated males (DMI) and thymoquinone treated males (TQ50 and TQ100) recorded no significant difference in serum insulin concentration when compared witheach other but they were significantly lower than that of intact control male rats (C), but the average means of these four groups were significantly higher than that of non - treated diabetic male rats (DM).Quantification analysis results of gene expression, performed by real - time RT - PCR, revealed that treatment with thymoquinone caused significant increase of mRNA expression levels of Reg3a, InsI, InsII, PDX1, Pax6, NeuroD1 and MafA genes during the studied period. Histological findings of thymoquinone treated pancreases revealed normal cellularity of islets of Langerhans and normal exocrine tissue except few congestion in it, whereas those obtained from non treated diabetic rats showed complet impairment of some islands and highly destructed of others. Normal hepatic architecture with the appearance of radiating shape around the central vein, has been shown in the section obtained from thymoquinone treated diabetic rats except few congestion, obvious regeneration and mitotic division in the nuclei of hepatocytes. Sections obtained from non treated diabetic male rats showed sever congestion, large thrombi in the hepatic tissue, and loss of hepatic architecture with sever hemorrhage, degeneration in hepatocytes, and dilation of sinusoids. Section obtained from kidneys of thymoquinone treated diabetic rats reveales normal renal convoluted tubules with normal epithelium of the tubules and high cellularity of glomeruli. Whearas those obtained non treated diabetic rat revealed dilation of renal convoluted tubules with necrosis in the epithelium of the tubules and sever hemorrhage in the renal tissue. Immunohistochemical results revealed that male rats drenched with thymoquinone registered higher scores of positive cells and intensity of staining compared with other diabetic (DM and I) groups. Hisological sections obtained from pancreases of control male rats showed actively stained islets of Langerhan's by immunohistochemistry with actively stained populations of beta, alpha, and delta cells, whereas those obtained from pancreases of non treated diabetic male rats showed damage of most cell populations and negatively stained for the few remaining beta cells. on the other hand, section obtained from pancreases of diabetic male rats treated with thymoquinone showed actively stained islets of Langerhan's by immunohistochemistry with actively stained populations of beta, alpha, and delta cells. While those obtained from pancreases of diabetic male rats treated with insulin, in the same stages of experiment, showed negatively stained beta cells and other cells of islets of Langerhan's. Histopathological findingsrevealed moderate improvement of pancreatic changes shown in both exocrine and endocrine (Islands of Langerhan's) parts. It can be concluded that drenching of 50 or 100 mg/ kg, bw, of thymoquinone from Nigella sativa seed has potent hypoglycemic effect in experimentally - induced diabetic mature male rats. As well as its positive role in elevating the expression level of Reg3a, InsI, InsII, PDX1, Pax6, NeuroD1

التعبير الكيميائي - النسجي - المناعي والجزيئي لجينات Inh - a وInh - ba وInh - bb في الاعضاء التناسلية لذكور جرذان الوستر البالغة وغير البالغة == Immunohistochemical And Molecular Expression of Inh - A, Inh - Ba And Inh - Bb Genes In Reproductive Organs of Immature And Mature Male Wistar Rats

اسم المؤلف: وداد عبد جواد التميمي
اسم المشرف: جبار عباس احمد الساعدي | عدنان وحيد محمد البديري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The present study has been carried out at the department of biology, College of Education, Al - Qadisiya University, Iraq, to investigate the immunological localization of transforming growth factors beta (inhibins and activins) subunits during immature and mature periods and its involvement in male reproductive physiology of rats.At pre - pubertal stage, five male rats of 25, 30, 35, 40, and 45 days old, and at post - pubertal stage, five male rats of 55, 60, 65, 70, and 75 days old have been used in the present study. Experimental animals have been anesthetized and blood samples were obtained from abdominal vein for assesment of activin - A, inhibin - B, FSH, LH, testosterone, and estrogen, testis volumes were measured for each age period. Testes, epididymis, prostate and seminal vesicle, were obtained for molecular and immunohistochemical studyto investigate the expression levels of Inha, Inhba, and Inhbb genes using qRT - PCR and immunohistochemical technique.The present study demonstrated gradual increase of testis volume throughout the male rat life in parallel with the increase of serum inhibin - B and testosterone concentrations. Serum activin - A concentration increased significantly at 30 and 40 day periods. Throughout the post - pubertal stage, activin - A concentration gradually decreased. Serum inhibin - B concentrations gradually decreased at the pre - pubertal stage. Post - pubertal stage registered gradual increase. At 25, 30, and 35 day periods, serum FSH level registered no significant changes, whereas 40 day period recorded significant increase then decreased at 45 day period. Throughout the post - pubertal period, the level of FSH concentrations continued in gradual decrease. At 25, 30, 35, and 40 day periods, serum LH and testosterone levels showed no significant differences, whereas 45 day period recorded significant increase. Postpubertal period showed gradual significant increase. Serum estradiol concentration decreased gradually at the pre - pubertal stage and continued in decrement at the post - pubertal stage.The expression level of Inha gene in testis decreased as the age progress until 40 day period, and then slightly increased at 45 day period. At 55 day period, the expression significantly increased. At 60 and 65 periods, the levels recorded no increase, but 70 and 75 day periods recorded significant increase. The expression level of Inhba gene increased significantly as the age progress at the pre - pubertal stage, where the highest level was recorded at 45 day. At 55, 60, and 65 day periods, the highest expression level has been recorded, thenafter, the levels decreased at 70 and 75 day periods. The expression level of Inhbb gene increased significantly at 30, 35, 40, and 45 day periods of the pre - pubertal stage. At 55 and 60 day periods, no significant difference was recorded compared with 45 day period. At 65 day period, the highest level was recorded, thenafter, the levels decreased at 70 and 75 day periods. The expression level of Inha gene in epididymis recorded no significant difference at all periods of the pre - pubertal stage, but the post - pubertal stage showed gradual significant increase as age progressed. The expression level of Inhba gene recorded no difference at 25, 30, 35, and 40 day periods, but it was significantly higher at 45 day period. The levels at 55, 60, and 65 day periods recorded no significant difference when compared with each other or with 45 day period. Significant increase has been recorded at 70 and 75 day periods. The expression level of Inhbb gene increased significantly at 30 and 35 but it decreased at 40 day period, then it showed further increase at 45 day period. At 55, 60, 65, and 70 day periods, also increased but the highest expression level was recorded at 75 day period.The expression level of Inha, Inhba, Inhbb genes in prostate recorded no significant difference at all periods of the pre - pubertal stage, but the postpubertal stage showed significant increase at all periods.The expression level of Inha and Inhba genes in seminal vesicle recorded no significant difference at 25, 30, 35, and 40 day periods and significant increase at 45 day period. The 55, 60, and 65 day periods recorded no significant difference when compared with each other but significant increase has been recorded at 70 and 75 day periods. The expression level of Inhbb gene recorded no significant difference at 25, 30, and 35 day periods, but it increased as the age progressed, whereas the levels showed no significant difference at all periods of the post - pubertal stage, but they were significantly higher than that recorded at the pre - pubertal stage. The results of immunohistochemical study demonstrated positive immunostaining for inhibin - ? subunit in Sertoli cells and primary spermatocyte and no staining in Leydig cells of pre - pubertal rat testis, whereas post - pubertal testis showed positive immunostaining in Sertoli cells, Leydig cells and primary spermatocyte at 55 day period and positive immunostaining in Sertoli cells, Leydig cells, and spermatogonia at 60 day period. There was positive immunostaining in Sertoli cells, Leydig cells, spermatogonia, primary spermatocyte and spermatid at 65, 70, and 75 day periods. At 25 and 30 day periods of pre - pubertal stage, rat epididymis showed moderate positive immunostaining in epithelial cells, but strong positive staining in epithelial cells has been shown at 35, 40, and 45 day periods and at all periods of the post - pubertal stages. The result expressed positive immunostaining in the epithelial cells of the pre - pubertal rat prostate and strong positive immunostaining at the post - pubertal stage. on the otherhand both, pre - pubertal and post - pubertal rat seminal vesicle showed strong positive immunostaining in epithelial cells. Weak positive immunostaining of Inhba has been observed in the primary spermatocyte and no staining in Sertoli cells and Leydig cells in the prepubertal rat testis and at 55 day period of the post - pubertal rat testis, and no staining in Sertoli cells, Leydig cells and spermatogenic cells at 60, 65, 70, and 75 day periods. Moreover there was positive immunostaining in epithelial cells in both the pre - pubertal and post - pubertal rat epididymis and prostate. In the post - pubertal stage, rat prostate showed strong positive mmunostaining in epithelial cells. No staining was observed in epithelial cells in both the prepubertal and post - pubertal rat seminal vesicle. The pre - pubertal rat testis showed strong immunostaining for Inhbb subunit in Sertoli cells, Leydig cells and primary spermatocyte, in adittion to spermatid in post - pubertal rat testis. Strong positive immunostaining in epithelial cells of the pre - pubertal rat epididymis at 25 and 30 day periods was expressed, but moderate positive immunostaining in epithelial cells of prepubertalrat epididymis was observed at 35, 40, and 45 day periods and postpubertal rat epididymis. However Inhbb subunit in both pre - pubertal and postpubertal rat prostate showed positive immunostaining in epithelial cells. There was positive immunostaining in epithelial cells in the pre - pubertal rat seminal vesicle at 25, 30, 35, and 40 day periods, and strong positive immunostaining at 45 day period and post - pubertal stage.It could be concluded that serum inhibin - B has positive correlation with testis volum and testosterone concentration at pre - and post - pubertal stages, and positive correlation with FSH and LH concentrations at pre - pubertal but negative partial correlation at post - pubertal stage. There was relationship between serum inhibin and activin concentration and fold changes of Inha, Inhba, and Inhbb genes in testis, epididymis, prostate, and seminal vesicle tissues at all periods of the study. Positive immunostaining for inhibin ? - and ?B - subunits, but not for ?A - subunit has been shown in testis cells and epithelial cells of seminal vesicle, positive immunostaining for inhibin ?, ?A, and ?B subunits in the epididymis and prostate