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تحسين التحمل الملحي لفسائل نخيل التمر (Phoenix dactylifera L .) صنفي البرحي والساير باستخدام بعض المركبات المضادة للملوحة

اسم المؤلف: حسين جاسم شريف
اسم المشرف: عباس مهدي جاسم مؤيد فاضل عباس
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - البستنة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:

تقويم تحمل بعض اصناف البطاطا Solanum tuberosum L للملوحة خارج الجسم الحي Evaluation of certain potato (Solanum tuberosum L.) cultivars for salinity tolerance in vitro

اسم المؤلف: اماني اسماعيل خليل طاهر
اسم المشرف: ماجد عبد الحميد ابراهيم عواطف نعمة جري
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - البستنة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The study was carried out at the laboratory of plant tissue culture - college of Agriculture - University of Basra during the period 15/3/2013to 20/07/2015 ,to study the effect of salinity stress on some growthindicators contain in callus produced from three potato varieties (Lizeta,Arnova and Safari) in Vitro.The sprout of the three potato varieties (Lizeta, Arnova and Safari) were cultured in aseptic condition on Murashige and Skoog (MS) full strength media supplemented with IAA and BA at concentration of 1.0 mg / L in addition to GA3 at concentration of 0.1 mg / L.For callus induction, the shoots were cultured on (MS) media upplemented with 3 mg / L NAA in combination with 1 mg / L Kin or BA.Moreover; to study the effect of salinity stress on some growth indicators , the callus exposed to NaCl at concentration (0,80, 100, 120 ,140 and 160 mM ) four weeks period, in additon to the effect of salisalic acid in combination with NaCl on protien charectirstic in callus of three potato cultivars. The results sammurized at follow : 1 - The effect of cultivars and growth regulators results sammaurized : No significant on regeneriation ,but Arnova cultivar was superior in height plant and number of shoots reached (7.27 cm and 2.48 shoot ) respectively.Lizeta was superior in number of leaves (1.59 leaves / plant ), the combination of hormons (1.0 mg/L of eachIAA & BA) was superior in height plant and number of shoots reached (2.1 cm and 1.6 leaves / plant ) respectively. 2 - Lizeta cultivar was superior in (% callus induction, callus fresh weight on 28 and 45 days ,CHO,protein,proline, Lipid peroxidation and K+ ion) parameters reached (31% ,186.25 mg, 303.9 mg, 29.09 mg /gm dry weight , 16.29 mg/gm dry weight 3.05 μ.gm /dry weight , 204 nmol MDA g/ fw and 9.16 mg/gm dry weight ) respectively .While Ornova cultivar was superior in (plant hight,shoots number, callus dry weight ) reached (7.27 cm, 2.48 shoot/ plant and 18.13 mg) respectively .3 - The combination of (1.0 mg / l ) of BA and IAA affected significantly on number of shoots and leaves parameters and give (2.1 shoot/plant ,1.61 leaf/ plant ) respectivelywhile (1.0 mg/l IAA+0.1 mg/l GA3 +1.0 mg/l BA ) affected significantly on shoots hieght which reached 8.03 cm .4 - Varied response were found among potato callus growth under 100 mM concentration of salt stress, Arnova and Lizeta were superior as compare with Safari which didn’t show any response in addition to callus growth reduction were found at high concentration (120, 140 and 160 ) mM of salinity . Moreover,100 mM gave negative responce on on callus fresh and dry weight .Furthermore, NaCl affected significantly on V.C and Lipid peroxidation at high concentration of NaCl as compare to control treatment .Concerninig to (CHO,protien,proline, Na - 1 and Cl - 1 ions containt ) , 100 mM of NaCl gave posetive responce for both Lizeta and Arnova cultivars as compere to Safari which in turn recorded highest rate at 80 mM concentration and less concentration of protien , K and K/Na ratio 5 - the interaction of 120 mM NaCl with 0.250 mM SA affected significantly on callus growth in Lizeta as compare with other tow cultivars which recorded no responce NaCl affected posetively on Gen expression, in callus of potatocultivars which represented in a form of a new protein bands with new molecular weight at 80 and 100 mM in addition to the interaction of NaCl with SA treatment in Lizeta cultivar. 7 - Significant responce were found among NaCl and potato cultivars in % of shoot initiation reached 42.11% for Lizita in control treatment and less responce in 80 mM reached 11.78 %. 8 - Sucsessful % of acclimatized plant for Safari reached 82.59% as compare to other cultivars while Lizita was superior in plant hight number of leaves, total root system length ,total yield , number and dimeter of tubers reached (28.33cm, 5.50 leaf/plant ,1.06 dcm2 ,10.25 cm, 17.63 g/plant , 5.16 tuber/plant, 3.18 cm) respectively . On the other hand Safari cultivar were recorded significant reduction in proline, CHO, protien reached (2.03 μ.gm/dry weight, 23.05 and 5.77 mg/gm dry weight) respectively.

تاثير موعد الزراعة وكثافتها وحامض السالسليك وتداخلاتها في نمو نبات الكزبرة Coriandrum sativum L. وحاصله من الثمار وفعاليته التثبيطية لبعض الاحياء الدقيقة Effect of Sowing date, plant density and Salicylic acid in Growth plant coriander Coriandrum sativum (L.) and their yield from fruit and inhibitory activities of some microorganism

اسم المؤلف: ليلى تركي فضالة البدري
اسم المشرف: سميرة عبد الكريم مطرود ماجد عبد الحميد ابراهيم
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - البستنة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The present study was conducted during the growing season of 2015/2016 in the Medical and Aromatic Plants Unit Field, Collage of Agricultural, Basrah University, to study the effect of sowing date, plant density and salicylic acid in growth and yield of Coraiandrum sativum (L and its inhibitory activities of some microorganismThe study Includes 12 factorial treatments, which were the combinations of two sowing dates 1/10 or 1/11, two plant densities 25 plant m - 2 or 50 plant m - 2 and spraying with salicylic acid in concentration of 0 ,7.5 or 15 mg.l - 1. A Complete Randomized Block Design in a factorial experiment was used with three replicates. Treatment means were compared according to the Least Significant Differences Test (L.S.D) in level 0.05 was used to compared with Genstat programs. The most important results may be summarized as follows : - 1. Effect of sowing dates : Plants sown on 1/10 gave significantly increases in plant height, leaf number. Plant - 1, number of main lateral branches.plant - 1, dry weights of shoot system.plant - 1, length and dry weight of root system date to the appearance of the first inflorescence, the total number of inflorescences. plant - 1, total number of mini inflorescences plant - 1.total, the productivity of fruit. hectare - 1 (8.96 ton),oil yield.plant - 1 (0.893g), productivity of oil.hectare - 1 (330 kg), refractive index of volatile oil, leaves content of total soluble carbohydrates, leaves content of vitamin C and fruit content of total soluble carbohydrates. Whereas plants sown on 1\11 had a significant effect in total of mini inflorescences, and total protein percentage in fruit.2. Effect of plant densitycultivated plant at 25 plant.m - 2 had a significantly effect in leaf number.Plant - 1, number of main lateral branches.plant - 1 , dry weights of shootSummaryBsystem.plant - 1, length and dry weight of root system , , total number of mini inflorescences.plant - 1, leaves content of total soluble carbohydrates, fruit content of total soluble carbohydrates. cultivated plant at 50 plant.m - 2 had a significant effect in plant height, the productivity of fruit hectare - 1 (8.56 ton), productivity of oil .hectare - 1 (320 kg).3. Effect of salicylic acidPlant sprayed with SA at 15 mg.l - 1 had a significant effect in number of main lateral branches, dry weights of shoot system.plant - 1, dry weight of root, the total number of inflorescences.plant - 1, total number of mini inflorescences plant - 1. weight of 1000 fruit, , refractive index of volatile oil, oil density, leaves content of total soluble carbohydrates, fruit content of total soluble carbohydrates, total protein percentage in fruit. Whereas Plant sprayed with SA at 7.5 mg.l - 1 had a significant effect in leaf number.Plant - 1, length of root system, and the productivity of fruit.hectare - 1 (8.83ton).4. Effect of the interactionsThe Interactions between the study factors, showed a significant effect in most studied parameters.5. Effectiveness of alcoholic extracts and oil of the plant coriander against the two types of pathogenic bacteria to humanThe activities of methanolic and ethanolic extracts and oil of fruit were tested against some types of bacteria Escherichia coli and Staphylococcus aureus at three concentrations100, 200, or 400 mg.l. - 1 by using filter paper disc diffusion method. Each of ethanolic extract and oil of the fruits inhibition in concentrations 400 mg.l - 1, were effected growth of bacteria E. coli. They reached the diameter of inhibition 12 mm. Ethanolic extracts of the fruits at concentration of 400 mg.l - 1 gave the highest inhibition of growth of bacteria staph. aureus amount 12mm and E. coli the diameter of inhibition 10 mm.SummaryC6. Effectiveness of alcoholic exteracts and oil of the plant coriander against two pathogenic fungi to humanThe activities of methanolic and ethanolic extracts and oil of fruit were tested against types two fungi Aspergillus niger and Candida albican at three concentrations 100, 200,or 400 mg.l - 1. Was mathanolic extract of the fruits at concentration of 400 mg.l - 1 gave the highest inhibition of growth of A. nigerand C. albican amount 12mm and showed oil fruit and ethanolic extracts at concentration cultivated of 400 mg.l - 1 the highest inhibition against A. nigerand C. albican amount 10mm.

تاثير رش التربتوفان والكلايسين واضافة المحفز الحيوي Biohealth للتربة في نمو وحاصل بعض اصناف البطاطا SolanumtuberosumL في الاراضي الصحراوية EFFECT OF SPRAYING TRYPTOPHAN, GLYCINE AND SOIL APPLIED BIOHEALTH ON GROWTH AND YIELD OF SOME POTATO (SolanumtuberosumL.)CULTIVARS IN DESERT LANDS

اسم المؤلف: عباس كاظم عبید عباس
اسم المشرف: عصام حسین علي الدوغجي نوال مهدي حمود
الموضوع العام: علوم الزراعة
السنة: 2016
الموضوع الدقيق: النبات - البستنة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Two experimentswere conducted during autum seasonsof 2013 - 2014 and 2014 - 2015 in Tomato Development Project belong to Basrah Agricultural Director, Khor Al - Zubairin southern Iraq to study the effect of foliar application of tryptophan, glycine and soil appliedBiohealthongrowth and yield of some potato cultivars. The aim of the experiments was focused on the effect of three potato cultivars (Draga, Arnova and Provento) in the first season and (Arizona, Arnova and Rivira) in the second season.Spraying with two concentrations( 100 and 200 mg.l - 1)for both Tryptophan and glycine and 0 mg.l - 1(sprying with distilled water).Spryingwas done twice 45 and 60 days after after propagating.Besides, additiontwo concentrations ofBiohealthinto the soil( 0 and 1 g.l - 1for the first seasonand 0 , 1 and 2 g.l - 1 for the second season). The addition ofBiohealthwas done twice 45 and 60 days after propagatingfor both seasons. The experiments was adopted as factional experiment.Split - Split Plot Design was used with three replication,Least Significant Differences Test(LSD) was used at probability of 0.05. The results could be summarized as follows : 1. Emergence of field parameters Results showed that"Arnova" cultivargave asignificant increases in speedemergence field after 20.51 and 19.43 daysin both season,respectively."Brovento"in first season and “Arezona” in the second seasongave asignificant increasesin the percent of field emergence 96.42 and 89.33%respectively, .2.Vegetative growth parameters Resultsshowed that"Draga"cultivar in the first season and "Rivira" inthe second seasonincreased significantly in mostvegetative growth parameters.Spryingwith 200mg.l - 1 tryptophan in bothseasons gave increased significantly in mostvegetative growth parameters.Application withbiohealth1 g.l - 1 in the first seasonand 1, 2 g.l - 1In the second season gave significant increases in mostvegetative growth parameters.The interactions between the studied factors caused significant increased effects in all vegetative growth parameters on both seasons. 3. Yields parameters Results in the first season showed that "Draga" cultivar gave significant increases in tuber number.plant - 1,total plant yield, marketable yield,total yield, small and large tubers yield (6.10 tuber,379.9g,3.36 ton.donum - 1, 3.71 ton.donum - 1,0.35ton.donum - 1 and2.52 ton.donum - 1,respectively), While "Rivira cultivar in the second season gave significantincreases in tuber weigh,total plant yield,marketable yield,total yieldand large tubers yield(88.38 g, 492.6g,4.50 ton.donum - 1,4.82 ton.donum - 1, 3.87 ton.donum - 1,respectively).Sprying with 200 mg.l - 1 tryptophan in the two seasons gavesignificant increases in tuber weigh (69.08, 74.95 g), total plant yield (389.1 , 438.4 g), marketable yield (3.54, 3.87ton.donum - 1),total yield (3.80, 4.28 ton.donum - 1) and large tubers yield(2.44, 3.08 ton. donum - 1) respectively, While the 100 mg.l - 1 tryptophan increases in tuber number.plant - 1 in the first season(5.76 tuber) and 200 mg.l - 1 tryptophan increases in the first seasonin yield medium tubers (1.10 ton.donum - 1)and in the second season increases in tuber number.plant - 1(5.90 tuber).Application with1g.l - 1 biohealthin the first season gave significant increases in tuber number.plant - 1,total plant yield,marketable yield,total yield,medium and large tubers yield(5.72 tuber, 366.6 g, 3.28 ton.donum - 1,3.59 ton.donum - 1,1.03 ton.donum - 1and 2.26 ton. donum - 1,respectively), while in the second season application with 2 g.l - 1 biohealthgave significant increases in tuber number.plantC 1,total plant yield,marketable yield,total yield,and large tubers yield(5.85 tuber, 423.4 g), 3.75ton.donum - 1 ,4.15 ton.donum - 1 and 3.00 ton.donum - 1,respectively). The interactions between the studied factors caused significant increased in allyields parameters on both seasons.4. Chemical characteristics 4 - 1. Leaves chemical characteristics Spraying with 100 and 200 mg.l - 1 tryptophan in Carbohydrate contents. In the second season200 mg.l - 1glycinegave significant increases in carbohydrate contents.Application with 1 g.l - 1biohealthin second season gave a significant increase incarbohydrate contents.The interactions between the studied factors caused significant increased effects in all leaves chemicalparameters on both seasons.4 - 2. Tubers chemical characteristics Results showed that "Brovento" cultivar in the first season gave significant increases in the percentage of dry matter, starch and specific gravity, "Draga" increases in thepercentage of protein, vitamin C and total solubilesoilds, "Arnova" increases intotal free amino acids. In the second season "Rivira" cultivar gave significant increases inthe percentage of dry matter, starch, specific gravity and total free amino acids and"Arezon" increases in thepercentage of vitamin C and total solubilesoilds.Foliar application with tryptophan at 200 mg.l - 1 in the first season caused an increase in the percentage of dry matter, starch,specific gravity and protein, while 200 mg.l - 1 glycine increases in vitamin C and total solubilesoilds, glycine at100 mg.l - 1increases in total free amino acids.Foliar application with tryptophan and glycine at 200 mg.l - 1in second season caused an increase in the percentage of dry matter, starch andspecific gravity, while 200 mg.l - 1 glycine caused increases in the percentage of protein and total free amino acids, glycine at100 mg.l - 1 increasesin vitamin C and tryptophan at 200 mg.l - 1 in total solubilesoilds.Application with biohealth gave asignificant increase in mosttubers chemical characteristics for both season.The interactions between the studied factors caused significant increased effects in all tuber chemicalparameters on both seasons.