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دراسة تاثير بعض المطهرات والمعقمات والمنظفات على نمو بعض الفطريات المعزولة من بعض المستشفيات في بغداد == Study the effect of some disinfectants, sterilizers and detergents on growth of some fungi isolated from Baghdad hospitals
Author name:
صادق رحيم محمد علي
Supervisor name:
منى حمودي الجبوري
General topic:
Biology
Specific topic:
Microbiology - Fungi
Degree:
Master
University:
University of Baghdad - College Of Science For Girls
Language:
English
University location:
Baghdad
First pages:
24T3358 - p.pdf
Abstract:
This study aimed to detect the fungal contamination of hospitals in orderto be controlled and limited.A total of 511 environmental swaps were collected from Al - Karamateaching hospital, Al - Sader general hospital, Al - Yarmouk teachinghospital, Baghdad teaching hospital and Imam Ali general hospital inBaghdad city from the period 28,October,2013 to 31,March,2014. Thesamples were collected to isolate and identify the caused and contaminatedfungi in addition to test their sensitivity against certain antiseptic anddisinfectants that are used in hospitals; also their virulence factors forhigher frequency and occurrence genus.These isolates were collected from main sources, the hospitalsenvironment and most of the items used for hospital work in order to detectthe nosocomial pathogens persistent in the hospital ecosystem, whichincludes : sink surfaces, tools and equipments, walls, indwelling medicaldevices, premature incubators, beds, medical trashes, floors and others.Results revealed that fungal contamination in five hospitals was in thepercentage 69.89%, 64.52%, 62.40%, 51.58% and 41.90% respectively. Inaddition, the most contaminated places were the hospitals wards 80%,while it was 77.77% in endoscopy units and 76.92% in the Respiratory careunits. The lowest contaminated places were the emergency units 25.0%then burns units 33.33% and cardiac care unit which was 41.66%. Inrespect with the percentage of occurrence and frequency for the isolatedfungi, it was found out that Aspergillus flavus formed the highest ratio offrequency and occurrence 21.29% and 15.46% respectively, followed byAspergillus niger 18.33% and 13.31% then Penecillium spp. which was10.78% and 7.83%. Regarding other fungi, their frequency and occurrence were different. All fungal isolates were identified depending on themorphological and microscopic examinations as well as biochemical tests.The results on the effect of temperature and pH on fungal growthshowed that the best growth was at 25 - 30 OC, and the best growth wasfound at the pH range 5.0 - 6.0.Results of the test of fungal sensitivity against eight antiseptics andsterilizers used in the hospital showed that the ratio of inhibition of thesecompounds agnaist fungi were different according to types andconcentration of antiseptics as well as genus and species of fungi. Use ofthese compounds in 100%, 50%, 40%, 30% and 20% concentration is veryhigher effective, while more effective if they were used in 10% or 7.5%concentration than in 5% and 2.5%, but not effective in 1.25%concentration.The results showed that Setol (Chloroxylenol) has significantly the mosteffective compound on fungi, followed by Formalin (Formaldehyde), thenCarmal (Tension, active surfactants, non - ionic) and Alwazir (Sodiumhipochlorite).Testing some of virulence factors aflatoxin production, lipaseproduction , protease production and haemolysis test, it was found that A.flavus has higher lipase production and protease production than otherfungi. A. flavus and A. niger were capable of blood hemolysis in 3 daysonly, but in different diameters of lysis zone, 1.8mm and 1.6mmrespectively. Some isolates of A. flavus are capable to aflatoxin production.Depending on all results, hospitals contain different fungal species andgenus, antiseptics and disinfectants have an effect on fungal growth if theyare used in a correctly.
