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المتغيرات الذوقية والشمية لدى اطباء الاسنان المصابين بمرض كوفيد-19 فيما يتعلق بالتغير الجيني ل FUT2 ومستويات الاجسام مضادة في المصل واللعاب == Gustatory and olfactory changes among dentists with COVID-19 in relation to FUT2 gene polymorphism, serum and salivary antibody titers
Author name:
فريال محمود عبد الرضا
Supervisor name:
امينة ريحان دعيجل
General topic:
Dentistry
Specific topic:
Dentistry
Degree:
Doctorate
University:
University of Baghdad - Faculty Of Dentistry - Department of Oral Medicine
Language:
English
University location:
Baghdad
Key words:
- covid-19
- Anti s and anti n specific to corona virus titers
- gustatory and olfactory loss
First pages:
T99727 - p.pdf
Abstract:
Background: Corona virus disease (COVID-19) manifested as acute respiratory distress syn-drome affected millions of people worldwide. Dentists are at increased risk of acquir-ing occupational infections. The infection susceptibility is linked with the both Anti-gen blood grouping system (ABO) and the alpha (1,2) fucosyl transferase (FUT2) se-cretor. Sudden onset of anosmia and/or ageusia symptoms could be enough to isolate patients and vaccinated participants even without doing the COVID-19 test. This study aimed to assess the changes in the chemical sense by one of objective methods the Connecticut Chemosensory Clinical Research Center Test (CCCRC). Assessed post-COVID Immuno-globulin A and G immunity, quantification of salivary biomarkers for prognostication, and immune surveillance. to find any association between specific ABO blood groups and FUT2 secretor status and COVID-19 in a sample of Iraqi den-tists. Methodology: For each 133 participants, a questionnaire including demography, COVID-19 status, blood grouping, Rhesus factor, with chemo sensitive symptoms was recorded using n-butyl alcohol and environmentally realistic odorants and calculated in a modi-fied way for the detection of olfactory function. The gustatory test scoring was done using the four standard primary tastes. A cross-sectional study was done on 91 out of 133 working Iraqi dentists from June to August 2022. The dual IgG and IgA for both spike and Nucleocapsid proteins (s1 & n) specific to COVID-19 were measured by En-zyme linked immunosorbent assay (ELISA)-specific kits from serum and saliva. For randomly selected 36 out of 91 participants Cluster of Differentiation 4 (CD4) and subtypes T helper 1 and T helper 2 were counted by flow cytometry from fresh whole peripheral blood. The DNA was extracted from salivary leukocytes for all study partic-ipants. Molecular detection of the FUT2 gene by real-time Polymerase Chain Reac-tion and the data were sequenced and the phylogenetic tree was drawn for randomly selected 20 participants. Results: participants were more commonly females (81,61%) with the majority were be-low 35 years of age (97,72%). Half of the participants experienced smell and/or taste loss during the COVID-19 infection; whereas (133;99.2%) recorded by CCCRC had olfactory dysfunction and (105,78.2%) had gustatory dysfunction. Males were with a higher significant rate of taste loss (P=0.009) whereas females with a higher recovery rate within the first two weeks after the onset than males. The Pfizer vaccine was sig-nificantly related to smell loss (P=0.038). All cells’ levels were reduced and signifi-cantly associated with a positive COVID-19 infection whereas the CD4 was signifi-cantly related to the Pfizer vaccine, loss of both sense and recovery time within 15 days. A high significant positive correlation was found between CD4 and Th2, IgG n in serum; was highly significantly related with positive COVID-19 infection higher than that of serum IgG s1. Noticeably, the IgA (s+n) in serum was negatively correlated with Th1 and Th2, could be detected in individuals with a duration from last infection >1-2 years and last vaccine duration > 6- 12 months. Blood group O was the most pre-dominant among this study participants (40%), followed by B, A, and AB with (90%) of them were RH+. A significant relationship between smell dysfunction and infected blood group A and RH+ (p =0.044, 0.038) while taste dysfunction was negatively and significantly correlated with AB group (r=-0.73; p=0.008). The FUT2 secretor showed a significant relation with COVID-19 infection and frequency. The majority of COVID-19-infected participants experienced a significant loss of both smell and taste with fast recovery within 2 weeks. Conclusions: The COVID-19 infection susceptibility and reinfection associated with FUT2 secretor status and greatly related to olfactory and gustatory sense loss. Sex variation was noticed with more predominant to females in dysfunction and faster recovery from dysfunction. The blood group A was associated with olfactory dysfunction and more with Pfizer vaccine. The low level of CD4 and an imbalance Th1/Th2 ratio made the recovered individual more susceptible to re-infection but the significantly high percentage of specific COVID antibodies followed one time of infection or booster vaccine dose gave their protection.
Summary:
1e8cbe1dd9.pdf
References:
ee80003662.pdf
