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عزل وتشخيص بعض انواع البكتريا المكسرة للهيدروكاربونات النفطية الاليفاتية في الترسبات النفطية لمحافظة البصرة == Isolation and Identification of some species of degradable Bacteria of N - Alkanes hydrocarbons compounds in oil sludge at Basrah Governorate
Author name:
استبرق عبد الحسين سلمان جدي البوغازي
Supervisor name:
ميثم ايوب عبد القادر الحمداني
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Baghdad
First pages:
24T3525 - p.pdf
Abstract:
In the oil industries, large amounts of oil sludge are generated through the refining of crude oil. The main reason for its composition that is when exporting crude oil various kinds of oils (heavy and light ones) are mixed together for the purpose of exportation. But , the mixing process between different types cannot be fully , due to the lack of homogeneity among different quality of crude oil . So, the heavy parts of oil are precipitated to the bottom of tank and with passage of time they accumulate to be sediments that’s known as oil sludge stated at the bottom of the oil storage tank in the cleaning process , oil tanks are emptied and discharged in some spaces or reservoir (pits) located near the tanks called "oil pits". Which are places of dump excess oil of various types of oil from different oil tanks or they are close places from oil / water separating unites .From these spots, samples of two kinds of oil sludge : the bottom of oil tank (during the maintenance of the tank) and oil pits (near the unities of separating water from oil) are collected. Also, samples of soil surrounding the oil pits from five oil locations in Basrah city are collected too. Those locations are : west of Gurna, Northern Rumela, Southern Rumela, Nahar Umar, Alzubeir.At the rate of redundantly for each location and for each type of the study samples.This investigation is considered as the first which conducted in Iraq which include firstly the isolation and identification different types of bacterial species from different oil sludge, and secondly use some of them in biodegradation studies for two kinds of carbon sources of oil sludge : (oil sludge of tank bottom , oil sludge of oil pits) .Oil degrading bacterial species have been isolated on a selective mineral salt medium Bushnell and Hass Mineral Salt Medium (BHMS), supplemented with a carbon source. They are also identified depending on the Morphology and biochemical characteristics; four genus of bacteria (out of 89 bacterial isolates of oil sludge from the tank bottom)Bare identified. They include : 32 Pseudomonas spp. , 27 Rhodococcus spp. 21 Bacillus spp. , 9 Arthrobacter spp. , Also four genera of bacteria (out of 101bacterial isolates of oil sludge from the oil pits) are identified. They include : 41 Pseudomonas spp. 27 Rhodococcus spp. , 22 Bacillus spp. 11 Arthrobacter spp. , , While 8 species and 3 genus of bacteria (out of 259 bacterial isolates of the soil surrounding the oil pits) are identified. They include : 41 Aeromonas spp., 39 Corynebacterium spp., 39 Pseudomonas aeruginosa, 36 Bacillus spp., 33 Micrococcus leteuc, 23 Enterobacter intermedius, 22 Pseudomonas fluorescens, 12 Micrococcus varians 7 Staphylococcus epidermidis, 4 Staphylococcus saprophyticus,3Staphylococcus aureus .The biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes and two compounds of Pristane and Phytane are measured. Also, the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes and the two compounds of Pristane and Phytane are measured for each four genus which were isolated from the oil sludge pits, and it is also done for one isolated genus from the soil surrounding the oil pits. The measuring process was fulfilled as a comparative study with the control flasks. Thus, the oil sludge (tank bottom ) was used as a source of carbone in the first experiment While, the oil sludge (oil pits) was used as a source of carbone in the second experiment . The incubate time of the experiment is 27 days during which a lost of the percentage of hydrocarbon compounds each 9 days are recorded by using( Capillary Gas Chromatography ). The results showed the biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes of the oil sludge (tank bottom ) with regard toCbacteria 73.8 % Pseudomonas spp., 70.3 % Rhodococcus spp. 65.7 % Arthobacter spp., 55.6 % Aeromonas spp.,51.5 % Bacillus spp., Whereas, the biodegradation percentage of the lost in the concentration of the hydrocarbon compounds of the normal alkanes of the oil sludge (oil pits) with regard to bacteria 80 % Pseudomonas spp., 74.9 % Aeromonas spp., 70.4 % Rhodococcus spp., 64.8 % Arthobacter spp.,55 % Bacillus spp., While , The results showed of the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes of the oil sludge (tank bottom ) with regard to bacteria 73.7 % Pseudomonas spp.,70.3 % Rhodococcus spp. 65.6 % Arthobacter spp., 55.3 % Aeromonas spp. ,51.2 % Bacillus spp., Whereas, The results showed of the biodegradation of mole percentage of the lost in the area of the hydrocarbon compounds of the normal alkanes of the oil sludge (oil pits) with regard to bacteria79.2 % Pseudomonas spp., 73.8 % Aeromonas spp., 69.2 % Rhodococcus spp.,63.7 % Arthobacter spp., 53.7 % Bacillus spp. It was found that there is approximately match between the biodegradation of mole percentage by using the area and those by using the concentration for each component .
