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التوصيف الجزيئي لبعض محددات الضراوة لبكتريا Klebsiella pneumoniae المعزولة من عينات سريرية مختلفة في محافظة بابل == Molecular Characterization of Some Virulence Determinants of Klebsiella pneumoniae Isolated From Different Clinical Samples in Babylon
Author name:
مسار عامر مرزة العماري
Supervisor name:
جواد كاظم طراد الخفاجي | ايفاد كريم عبد الشبلي
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Master
University:
University of Babylon - Faculty Of Medicine
Language:
English
University location:
Babylon
First pages:
19T1267 - p.pdf
Abstract:
A total of 200 samples were collected from different sources of patients that including (sputum , tonsil, sinuses, vagina, urine) and from both sexes during a period from September 2016 to December 2016. Only twenty isolates (10%) were identified as Klebsiella pneumoniae positive according to the cultural characteristics and biochemical tests. Eight (40%) isolates from sputum, 5(25%) isolates from urine, 3(15%) isolates from tonsil, 2(10%) isolates from sinus, and also 2(10%) isolates from vagina. K. pneumoniae in males formed 12 isolates(60%) and 8 isolates(40%) in the females .The age - groups of patients who involved in the study was ranged from one year to thirty years old. The results showed that the mean age of patients was 14.9 years with standard deviation of 10.96. All isolates (100%) were able to produce capsule, siderophore, colonization factor antigen(CFA I/CFAIII) and urease, but none of these isolates were able to produce hemolysin. They have ability to form biofilm and have a strong role in stone formation. Regarding antibiotic susceptibility testing all Klebsiella pneumoniae isolates were resistant to Aztreonam, Ampicillin, Amoxicillin/ clavulanic acid) and penicillin G, whereas sensitive to Gentamycin, Ceftriaxone, and Amikacin. The molecular detection of some virulence genes ( magA gene, kfu gene, and uge gene) was done by PCR technique. And all isolates (100%)were negative mag A. Also, PCR technique displayed that 5 isolates 20(25%) were positive for Kfu gene, while only two isolates 20(10%) were positive for Uge gene. As well, the antibiotic resistance genes bla TEM and bla SHV markers were investigated, 18(90%) isolates positive to bla TEM from them(14) isolates carried on plasmid and (4) isolates carried on chromosomal DNA, while bla SHV gene was found in 17(85%) isolates positive from them (14) isolates carries on plasmid and (3) isolates carried onchromosomal DNA. Moreover, RFLP/PCR analysis explored bla TEM and bla SHV genotyping for Klebsiella pneumoniae isolates in the current study illustrating ESBL contribution in the increment of bacterial resistant to antibiotics.
