Share
العلاقة بين جين الانزيم المحول للانجيوتنسين متعدد الاشكال والانجيوتنسين 2 والانزيم المثبط لمحفز البلازمينوجين في المرضى الرجال المصابين باحتشاء العضلة القلبية وارتفاع ضغط الدم == The Association Between Angiotensin Converting Enzyme Gene Polymorphisms With Angiotensin II and Plasminogen Activator Inhibitor Levels in Male Patients With Myocardial Infarction And Hypertension
Author name:
فلاح حسن محمد حسن
Supervisor name:
مها فاضل سميسم | مشتاق وتوت
General topic:
Medicine
Specific topic:
Clinical Biochemistry
Degree:
Master
University:
University of Babylon - Faculty Of Medicine
Language:
English
University location:
Babylon
First pages:
19T1714 - p.pdf
Abstract:
Myocardial infarction (MI) is a necrosis of myocardial cell secondary to interruption of coronary blood supply caused by ischemia, Myocardial ischemia occurs when the oxygen supply to the heart is not sufficient to meet metabolic needs. The most common underlying cause of myocardial ischemia is obstruction of the coronary arteries by atherosclerosis.Many genetic factors that affect Blood Pressure, these Genetic factors include an inappropriately high activity of the Renin - angiotensin - system (RAS) and susceptibility to the effects of salts in the circulation.The aim of the study is to evaluate the role of Angiotensin converting enzyme - 1 (ACE - 1) polymorphism on the level of angiotensin II (Ang II), and the relation between Plasminogen activator inhibitor - 1 (PAI - 1) concentration and Ang II in (MI) and hypertension patients.This study was conducted in Hilla city, from the 1st of November 2016until 25th of February 2017. The MI samples were collected from the coronary care Unit (CCU) in Marjan Teaching Hospital in Babylon / Hilla city and Shaheed al mehrab center. The patients with hypertension sample were collected from Advisory Unit in the Marjan hospital. The control subject samples were collected from out of the hospitals.The study includes (100) males ageing between (50 - 60 years) and BMI (18.5 - 24.9) : 25 control, 75 patient groups, the groups of patient subdivided into {25 hypertensive patients for 10 to 15 years (group A), 25 hypertensive patients for ≥20 years (group B), 25 patients with MI plus hypertension (group C)}.XIIThe sera obtained from the blood of all groups were used to measure the concentrations of plasminogen activator inhibitor 1, angiotensin II, and lipid profile, while whole blood samples from study subjects were used to extract DNA for the study of polymorphisms in the angiotensin converting enzyme gene.The results show : 1. There is a significant increase in the mean of Ang II, PAI - 1 in groups (A, B and C) as compared to the control group, (p<0.01), but there was no significant difference between A and B groups2. The significant increase (P <0.01) in total cholesterol (TC), TG, LDL - C and VLDL - C, while serum HDL - C concentration was found to significantly decrease (P<0.01) in A, B and C groups as compared with the control groups, and no significant increase or decrease in lipid profiles in comparison between A and B groups.3. Groups (A, B and C) show significant positive correlations (p<0.01) (R = 0.7848, R= 0.6855, R = 0.8871) of Ang II concentration with PAI - 1 concentration respectively.In the genetic part of the study, genotypes angiotensin - converting enzyme gene determined by allele specific polymerase chain reactionThe result observed significantly increase of ACE - 1 deletion - deletion (DD) in group (C) (88%) compared with the control groups (44%), while there were no significant difference in the groups (A, B) compared with the control group, also the result showed ACE - 1 (DD) genotype was (66%),XIIIinsertion - deletion (ID) genotype was (34%) and ACE insertion - insertion II genotype was (0%) in all the study groupsConclusion : the (DD) genotype of the Angiotensin - converting enzyme - 1(ACE - 1) polymorphism was the most frequent among Babylon patients with myocardial infarction. This suggests that Babylon individuals with the (DD) genotype might be at high risk for myocardial infarction. Furthermore, D allele of the ACE - 1 polymorphism might confer increased risk for MIPlasminogen activator inhibitor - 1 (PAI - 1) may consider as a risk factor for myocardial infarction. And there was a positive correlation between Ang II and PAI - 1in all patient groups. Therefore DD genotype is lead to an increase of PAI - 1 level.
