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الطريقة المثلى لاستخلاص الدنا من عينات الدم البشري == Optimization Method of DNA Extraction From Human Blood Samples
Author name:
انتصار هادي طالب اليساري
Supervisor name:
ذكرى عبد العالي الكعبي
General topic:
Biology
Specific topic:
Criminal Evidence
Degree:
Higher Diploma
University:
University of Babylon - College Of Science - Department Of Biology
Language:
English
University location:
Babylon
Key words:
- History of Forensic DNA
- Sources of DNA (Evidence)
- Bone and Teeth
- Extraction of DNA
- Other Method for Investigation Human DNA Profile
- Instruments and Equipment
- Solutions and Agarose Gel Preparation
- Detection of β - Globin Gene by PCR Assay
- I
First pages:
24T3052 - p.pdf
Abstract:
his study was conducted to create an environment that mimic those samples collected from crime scenes Blood samples were collected from one hundred and twenty persons from Hila city. These samples were used to stimulate the different possible blood samples obtained for forensic medical purpose. The following simulations were created : 20 fresh blood samples, 20 frozen blood samples, 40 dried blood stain samples on carpet (20 samples collected after 2 hours & 20 samples collected after 3 hours), and 40 samples in the form of dried blood stains on soil(20 samples collected after 2 hours & 20 samples collected after 3 hours).Extraction of genomic DNA was attempted from the listed forms of blood samples by using four different methods : Promega purification kits, Favorgen purification kits, salting out methods, and two - step lysis assay.These methods were compared for the best extraction of DNA and were confirmed by molecular detection of β - globin gene using polymerase chain reaction (PCR) technique.After extraction of gDNA and electrophoresis by use agarose gel electrophoresis and measuring the concentration and purity of DNA by bio drop spectrophotometer. Purity was then confirmed by PCR. The results indicated that the best methods for genomic DNA isolation were from fresh, frozen ,and dried stains on carpet using Promega purification kits, whereas, from dried blood stain on soil ,none of the four methods was suitable for isolation the genomic DNA.
