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التشخيص البكتريولوجي والمناعي لتجرثم الدم لدى المرضى الراقدين في وحدات العناية المركزة في مستشفيات مدينة الموصل == Bacteriological And Immunological Diagnosis Of Bacteremia In Patients Admitted To Intensive Care Uints in Mosul City Hospitals
Author name:
محمود ياسين يونس حسن البدراني
Supervisor name:
هيام عادل ابراهيم الطائي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Al Mosul - College Of Science
Language:
English
University location:
Mosul
Key words:
- تجرثم الدم
- التشخيص المناعي
- العناية المركزة
First pages:
T91295 - p.pdf
Abstract:
The current study was carried out in Nineveh Governorate and aimed to . investigate Bloodstream infections(BSIs) with Gram-positive and Gram-negative . bacteremia in patients admitted to intensive care units(ICUs) which consist of two . units, respiratory care units(RCU) and cardiac care unit(CCU) in Mosul City . Hospitals(Ibin Sina Teaching Hospital, Mosul General Hospital, and AL-Salam . Teaching Hospital). (90) blood samples both of sexes(male and female) for different . age groups ranging from (21 to 90) years were collected from RCU and CCU . patients between the first of July and December of 2022, used to separate serum and . plasma for immunological parameters. . In addition, (24) blood samples from healthy individuals served as a control . group. To documenting clinical data such a high fever, dyspnea, coughing, chest . pain, and risk factors accurate information form has been created.. After culturing (90) blood samples under aerobic and anaerobic conditions, final . laboratory diagnosis of bacterial isolates were done by using Vitek-2 compact . system at Mosul General Hospital.. The results indicated that 66/90(73.33%) blood samples revealed negative . cultures and 24/90(26.67%) blood sample revealed positive cultures consisting of . 14/24(58.34%) Gram-positive bacteremia were represented by Staphylococcus. . aureus 3/14(12.5%) and Staphylococcus. hominis also accounted for 3/14(12.5%), . they were predominant isolate followed by Staphylococcus.epidermidis. 2/14(8.33%), Staphylococcus. lentus 1/14(4.166%), Streptococcus. pseudoporcinus. 1/14(4.166%), Enterococcus. faecalis 1/14(4.166%), Enterococcus. faecium. 1/14(4.166%), Aerococcus.viridans 1/14(4.166%), and Gemella.morbillorum. 1/14(4.166%). . Whereas 10/24(41.66%) Gram-negative bacteremia were represented by. Klebsiella.pneumoniae 2/10(8.33%), Serratia. marcescens2/10(8.33%), Escherichia. . coli 2/10(8.33%), Pseudomonas. aeruginosa 1/10(4.17%), Burkholderia. cepacia. 1/10(4.17%) and Acinetobacter. Baumannii 2/10(8.33%). Antimicrobial susceptibility test(AST) and minimum inhibitory concentrations . (MIC) of all isolates were carried out by using the Vitek-2 compact system. The. determination of the minimum inhibitory concentration value ranged between . (0.12 to up to 320μg/ ml) for all antibiotics used in this study for Gram-negative and. Gram-positive bacterial isolates which had resistance to these antibiotics.. The study showed that Vancomycin, Linezolid, Tetracycline, Tigecycline,. Imipenem and Rifampicin provided the best antibacterial effect against most Grampositive bacteremia while Ceftazidime/Avibactm, Ceftolozane/Tazobactam, . Meropenem, Imipenem and Colistin is a good choice to treat bloodstream . infections(BSIs) with Gram-negative bacteremia.. The result of a device CBC Sysmex showed high level of white blood cells . (WBCs) count in blood of bacteremia and non-bacteremia patients group compared . with healthy control group high significant difference P-value (<0.001), whereas . level of WBCs in blood of bacteremia patients group compared with non-bacteremia . patients group showed high significant difference P-value (<0.001).. The result of Mini-vidas technique showed high level of D-dimer in plasma of . bacteremia and non-bacteremia patients group compared with healthy control group. high significant difference P-value (<0.001), while level of D-dimer in bacteremia . patients group compared with non-bacteremia patients group showed high . significant difference P-value (<0.001).. This study also included determination the level concentration of (IgM), (IgG). and (hs-CRP) in the serum of the study group by using Enzyme-Linked . Immunosorbent Assay(ELISA) technique. The results of ELISA technique showed. high levels of IgM and hs-CRP in the serum of bacteremia and non-bacteremia . patients group compared with the healthy control group with high significant. difference P-value (<0.001), whereas mean levels of IgG in the serum of bacteremia. and non-bacteremia patients group compared with the healthy control group showed. no significant difference P-value (>0.05). IgM, IgG and hs-CRP in patients who . have bacteremia compared with patients who do not have bacteremia showed no . significant difference P-value (>0.05).
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