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تقييم دور مؤشرات حيوية مختارة لدى مرضى التصلب المتعدد == Assessment Of The Role Of Selected Biomarkers In Multiple Sclerosis Patients
Author name:
ميساء نجاح امين
Supervisor name:
عبد العظيم ياسين عبود البراك | سرمد عبد الرسول الماشطة
General topic:
Medicine
Specific topic:
Microbiology - Immunology
Degree:
Master
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
First pages:
19T1149 - p.pdf
Abstract:
التصلب المتعدد (MS) هو من امراض الجهاز العصبي المركزي الاكثر شيوعا الذي يسبب الاعاقه الدائمه لدى الشباب البالغين. بناء على ادلة غير مباشرة قوية، يعتبر مرض التصلب المتعدد اضطراب المناعة الذاتية لعضو محدد ولكن لا يزال هناك الكثير يجب ان يفهم عن كيفيه نشوء ا | Multiple sclerosis (MS) is the most common disease of the central nervous system that causes permanent disability in young adults. Based on strong circumstantial evidence, MS is considered to be an organ - specific autoimmune disorder, but much remains to be understood about the initiation of the disease. It seems unlikely that MS results from a single causative event, but rather the disease develop in a genetically susceptible population as a result of environmental exposures. The present study was planned to determine the serum levels of IL - 17A, IL - 21 and Myelin Oligodendrocyte Glycoprotein (MOG) in patients and control subjects by a sandwich ELISA test using commercially available kits. Vitamin D total was measured in a group of patients and control subjects by cobas e 411 analyzers which is an automated multichannel analyzer for immunological analysis by the use of electrochemiluminescence (ECL) technology.Forty Iraqi MS patients attending the Consultation Clinic for Multiple Sclerosis / Baghdad Teaching Hospital during the period January 2013 - July 2013 were recruited for this study. For the purpose of comparison, 40 control subjects matched by age and gender were also included.The mean serum level of MOG in patient and control (554.85 vs. 315.20pg/ml) and IL - 17A (11.42 vs. 8.33pg/ml) was significantly higher in MS patients as compared to controls, while the mean serum level of IL - 21 (176.164 vs. 75.714pg/ml) was not significantly higher in MS patients as compared to controls. The mean serum level of vitamin D (n=20) (10.38 vs. 13.06ng/ml) was not significantly lower in MS patients as compared to controls. There was a positive correlation between MOG with IL - 17A and between MOG with IL - 21. These results might illustrate the synergism of action between antibody and T cell response in MS patients. A positive correlation was also detected between IL - 17A and IL21 in MS patients.A significant negative correlation was detected between Vitamin D with MOG. A negative correlation was recorded between Vitamin D and IL - 17A in patients. Also negative correlation was recorded between vitamin D and IL - 21 in MS patients. In conclusion, higher level of IL - 17A and MOG in MS serum indicates an important role of this cytokine and MOG as an auto antigen in the pathogenesis of multiple sclerosis and demyelination process, so they could be considered as a good biomarkers for diagnosis and considered as a target for future therapy.
