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تقدير بعض الاحماض الامينية بطريقة الحقن الجرياني - البريق الكيميائي == Quantification of Some Amino Acids By Flow Injection - Chemiluminescence Technique
Author name:
نشوان حسين علي سليمان الجبوري
Supervisor name:
سهام توفيق امين | نزار احمد ناجي
General topic:
Chemistry
Specific topic:
Analytical Chemistry
Degree:
Master
University:
Tikrit University - College Of Science - Chemistry Department
Language:
Arabic
University location:
Salahaddin
First pages:
25T1522 - p.pdf
Abstract:
شمل الفصل الاول مراجعة عامة تضمنت الاحماض الامينية، الخواص الكيميائية والفيزيائية للاحماض الامينية. كما تضمن الفصل الطرائق المستخدمة لتقدير (السستائين, السستين,الهستدين) بتقنياتها المختلفة، كما تم التطرق للبريق ولانواعه، والظروف الاساسية لحدوثه في الاطوار | This thesis are in four chapters : Chapter I : General survey for amino acids, and their chemical and physical properties. it also includes the methods used for the determination of (cysteine,cystine,histidine) with different techniques. This chapter covers different chemiluminescence types and the necessary conditions to happen in different phases (liquid, gases and solids). The chapter also includes the analytical application for chemiluminesccence reaction with luminol as regarded the donor molecules; also other molecules that has been used for determination of chemiluminesccence and was addressed Flow injection analysis, and application, Chapter I ended with the aim of the project.Chapter II : Includes the preparation of the major chemical reaction solutions that are required for this research work, and also many other solutions that were needed as stook solutions for the many detailed study that were conducted through this research work. The primary study system innovation (Electronic - chemical optical) for the measurement of chemiluminescne.Chapter III : It is three sections : Section A : Involves the study assessment of cysteine through all chemical variables for all involved chemicals in the reaction, ,also various physical parameters were studied,the optimum condition for the determination of system , based on the system chemiluminescence (luminol - hydrogen peroxide - cysteine), the concentration of cysteine over range of (5 - 110) µg.mL - 1.The limit of detection was 9×10 - 6 mol.L - 1. ,RSD % for five replicates of a standard (4.9×10 - 4) was 0.8%.Section B : The optimum condition for the determination of cystine , based on system chemiluminescence (luminol - hydrogen peroxide - Cu II - cystine), calibration was linear between (20 - 80) µg.mL - 1for cystine.The limit of detection was (1.6×10 - 6) mol.L - 1 ,RSD % for five replicates of a standard(2.08 x10 - 4) was 0.94 %. Section C : The optimum conditions the determination of histidine , based on the system chemiluminescence for (luminol - hydrogen peroxide - Mn II - Histidine), calibration was linear between (10 - 160) µg.mL - 1for histidine.The limit of detection was (9.2×10 - 6)mol.L - 1.RSD % for five replicates of standard (5.1 × 10 - 4) mol.L - 1 was 1.27%.All the proposed methods have been applied successfully for the determination of amino acids in pharmaceutical. Moreover, the accuracy and validity of those methods were evaluated against pharmacopoeia method using F and t - tests. It was found that experimental F and t values at 95% confidence level did not exceed the critical values indicating that the present proposed methods have a good accuracy and validity.Chapter IV : Deals with inferences and recommendations
