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مستوى الادنوسين دي امينيز وعلاقته بالجينات في مرضى التهاب المفاصل الرثوي == Adenosine Deaminase Level And Its Related Gene In Rheumatoid Arthritis Patients
Author name:
هديل علاء عبد الامير
Supervisor name:
علي حمود السعدي | سهير عيسى القيسي
General topic:
Medicine
Specific topic:
Clinical Biochemistry
Degree:
Master
University:
University of Babylon - Faculty Of Medicine
Language:
English
University location:
Babylon
First pages:
19T1677 - p.pdf
Abstract:
Rheumatoid arthritis is an inflammatory, autoimmune disease that causes pain, joint stiffness - especially in the morning - and loss of function. Although there are many forms of arthritis, of those commonly known, rheumatoid arthritis is the most serious and the second most common (after osteoarthritis). It can occur at any age but is more common in persons over the age of 30 years and affects women more than menObjective : Comparing changes in adenosine deaminase activity and C - reactive protein among patients with rheumatoid arthritis and control group , Comparing total anti - oxidant for patients with rheumatoid arthritis and control group , Determination of genes association with the occurrence of rheumatoid arthritis .Subjects and Methods : The study was conducted in rheumatology unit in Marjan teaching hospital in Hilla city / Babylon / Iraq from 1st ∕ December / 2014 to 1st / February / 2015.The present study was conducted on (60) patients with rheumatoid arthritis and (53) apparently healthy subjects were taken as a control group and the ages between (30 - 79) years. The sera obtained from the blood of patients and healthy subjects were used to measure the concentrations of adenosine deaminase , C - Reactive protein and total antioxidant capacity , while whole blood samples from study subjects were used to extract DNA for the study of polymorphisms in ADA1 gene and ADA2 and ADA6 gene.The patients and control groups enrolled in this study have undergone full assessment that included : - clinical assessment by history and examination (joints movement , presence of rheumatoid nodule,assessment of patient general condition) , assessment of the level of physical activity; hematological assessment {Red blood corpuscles counts (RBCs), blood hemoglobin (Hb) and packed cell volume (PVC), white blood cell (WBCs) count and erythrocyte sedimentation rate (ESR).Results : In comparison with the control group, the patients with rheumatoid arthritis showed a significant increase in of adenosine deaminase (P ≤ 0.05) and C - reactive protein (P ≤ 0.05) concentrations, and a significant decrease in total antioxidant capacity concentration (P ≤ 0.05) .Regarding to hematological parameters, the results of study show that there is significant increase (p ≤ 0.05) in ESR in RA patients with mild anemia. White blood cell count was slightly higher with no significant difference (p ≥ 0.05) between RA patients and control group.In the genetic part of the study , Genotypes of ADA1 gene determined by PCR technique were defined as AA , BB and AB according to the presence of the A (deletion) and B (insertion) alleles. There was statistically significant difference in both the genotypic distribution and allelic frequency between the patients versus healthy controls (odds ratio = 1.73 , CI 95% 0.792 - 3.800) of the AA genotype and A allele making them consider as non dependent risk factors for RAAccording to ADA2 gene , genotypes determined by PCR technique were defined as TT, TC and CC . There was statistically significant difference in both the genotypic distribution and allelic frequency between the patients versus healthy controls (odds ratio = 5.00 , CI 95 % 0.96 - 25.94) of the TT genotype and T allele making them consider as a dependent risk factors for RAAccording to ADA6 gene , genotypes determined by PCR technique were defined as DD, DE and EE There was statistically significant difference in both the genotypic distribution and allelic frequency betweenthe patients versus healthy controls (odds ratio = 2.177 , CI 95% 0.93 - 5.07) of the consider as a dependent risk factors for RASeveral genetic polymorphisms of certain genes ( such as ADA1 , ADA2 and ADA6 genes can have an effect in predisposing the subject to rheumatoid arthritis
