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دور الطفيليات والبكتريا مع بعض من عوامل الخطورة المرتبطة بها في تفاقم متلازمة القولون العصبيى == The Role of Parasites and Bacteria with some of Risk Factors in Exacerbation of Irritable Bowel Syndrome
Author name:
نور محمود سلطان
Supervisor name:
عبير عباس العطار | حيدر جليل مردان
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Master
University:
Northern Technical University - College of Health and Medical Technologies - Department of Medical Laboratory Technology
Language:
English
University location:
Kirkuk
Key words:
- Irritable Bowel Syndrome
- Bacteria
- Parasites
Abstract:
Consistently affecting the digestive system and resulting in cramping in the abdomen, Irritable Bowel Syndrome (IBS) is a prevalent chronic health problem. Constipation, excess gas, bloating, diarrhea, and bowel movements that may be harder or looser may affect anybody at any age and are often a life time issue. The correlation between IBS and several parasites and Bacteria. One hundred thirty (130) stool samples and blood from both sexes and their age between (15- <75) years were collected and (20) from healthy as control for some period and range of age. They were attended gastrointestinal clinic in Kirkuk/ Iraq from November 2021 to May 2022. Macroscopic examination like color, odor, consistency and mucoid etc. microscopic examination that used iodine, flotation and concentration method were used to identify the parasites that found in the stool in IBS patients. Many different media culture as MacConkey agar, Eosin Methylene Blue and Salmonella-Shigella agar and some biochemical tests were used to identify the species of bacteria that may be concerned with IBS. The results showed that only 54 (41.53%) of parasites isolated from patients with IBS were belonged to Blastocyst hominis, Entamoeba histolytica, Balantidium coli, Pentatrichomonas hominis, Giardia lamblia, Entamoeba coli, Enterobius vermicularis, Taenia saginata, Ascaris lumbricoides, Trichuris trichura in different percentage in the patients stool results showed that, only 109 (83.846%) of bacterial pathogenic isolated from patients with IBS were belonged to pathogenic Salmonella spp., Shigella spp., Escherichia coli, Helicobacter pylori, and Klebsiella spp. and 21 (16.15%) of the isolates were found to be normal flora from patients with IBS. The higher percentage 20 (15.384%) of parasite was belonged to Blastocyst hominis while thelowest percentage 1(0.769%) of parasites was found to belonged to Taenia saginata. The higher percentage of bacteria that was present in IBS patients was pathogenic Escherichia coli at 37 (28.461%) and the lower percentage was Helicobacter pylori at 3 (2.307%). Depending on some factors like sex, females were more affected with parasite than males, while in the bacterial infection males were more infected with bacteria than females. Individuals with age more than (46) were more infection with parasites than other age, while in bacteria, the most infection individual with age (36-55) years. Education and smoking were had more effect on the parasites and bacteria presence. The current study also used some of the hematological parameters. Including White blood cells, neutrophils (Neu), and lymphocytes (Lym) which were all included in a complete blood count (CBC) and were elevated in infection with parasites, but the hemoglobin (Hb) was decreased. The C-Reactive Protein (C-RP) that also elevated in IBS infected with parasites and bacteria. Interleukins were different in levels according to the type of infection.
Summary:
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