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دراسة فعالية نبات عين البزون كعلاج طبيعي لمرض سرطان الدم المستحث في ذكور الفئران == Study the Activity of Catharanthus Roseus as Natural Treatment for Induced Leukemia in Male Mice
Author name:
محمد عبود عياش
Supervisor name:
مفيد جليل عوض | نسرين جلال محمد
General topic:
Medicine
Specific topic:
Clinical Biochemistry
Degree:
Master
University:
University of Babylon - Faculty Of Medicine
Language:
English
University location:
Babylon
First pages:
19T1213 - p.pdf
Abstract:
Leukemia is cancer of blood - forming tissues, begin in the bone marrow characterized by highly elevated of abnormal proliferation and circulation of immature clonal hematopoietic cells. It can affect all ages around the world which in turn considered as one of public health problem.Plants considered as an important source for medical drugs manufacturing due to the presence of certain compounds with medicinal biological activity, therefore adopted in the preparation of a lot of medicines and medical drugs because of its' speed therapeutic influence and low of negative side effects which caused by chemically manufactured medications.The present study aimed to evaluate the activity and efficiency of vincristine and other important constituent in Catharanthus Roseus plant in treatment of leukemia through enhance and direct impact on some of the body's vital biological activities by using fresh crude plant as herbal medicine. This study is Cohort study design. It is monitoring, detection, and investigation study that included some of groups that completely under control; this study used in medicine, nursing, psychology and social sciences in addition to the Ecology. This study included (60) Albino male mice divided into four groups (15) mice for each. Group (A) was the control group, group (B) was mice feed orally with of C. Roseus plant, group (C) was leukemia induced mice by subcutaneous injection of (300mg/kg B.W benzene), and group (D) was Induced leukemia mice treated by oral feeding with C. Roseus plant. The experimental work was carried out in Clinical Biochemistry Department / College of Medicine / University of Babylon during the period from (October 2015 to August 2016).High - Performance Liquid Chromatography (HPLC) technique was used for measurement of Vincristine drug (VCR) in plant and vitamin D levels in mices' serum, Enzyme Linked Immunosorbent Assay (ELISA) had was used for the measurement of serum ferritin, UV - Vis Spectrophotometer was used for determination of GSH - Px and GST concentrations, while Graphite Furnace Atomic Absorption Spectrophotometer technique (GFAAS) was used for measurement of trace elements concentration of (Fe, Cu, Zn, Se). There were hematological and histopathology studies carried out by using special required equipment to make sure that leukemia disease was indeed occur.Hematological study included measurement of Hb levels and WBC count, while histopathology study included bone marrow, liver, kidney, spleen smear. Microscopic investigation for Chromosomal Aberration and Micronuclei were also had been done.Results showed that C. Roseus plant contain the concentration of vincristine drug up to 148μg/gm. There was highly significant increase of serum ferritin levels, GST activity, Iron and Copper concentration, and WBCs in leukemic group (C) (P<0.001) when compared with control group (A), while there were highly significant decrease of these parameters in group (B) as compared with the control (A) and (C) groups.Results also showed a highly significant decrease of GSH - Px activity, Vit.D levels, Zinc and Selenium concentration, and Hemoglobin levels in group (C) (P<0.001) when compared with the control group, while there were highly significant increase of these parameters in group (B) as compared with control and (C) groups. The present study found a positive correlation between Vit.D and GSH - Px activity, Ferritin and GST activity, while this study found a negative correlation between Ferritin and GSH - Px activity, serum Iron and Selenium concentration.The present study found positive effect of C.Roseus on abnormal parameters and support the biological system which was clear in group (D) which demonstrated that there were significant inhibitions to decrease or increase of these parameters than those in healthy control.
