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استكشاف الجهد لانتاج صور التاثير المراتي الالكتروني من عينات بيولوجية == Exploring the Potential for Producing Electron Mirror Effect Images from Biological Samples
Author name:
محمد شاكر موسى
Supervisor name:
حسن نوري عبد الوهاب العبيدي | دنيلا كانديا
General topic:
Physics
Specific topic:
Electronics Physics
Degree:
Master
University:
Mustansiriyah University - Faculty Of Education
Language:
English
University location:
Baghdad
First pages:
26T1923 - p.pdf
Abstract:
An experimental study which is based on theoretical aspects is adopted to investigate the most important parameters that control the electron mirror effect phenomenon in biological samples. The machine that has been used to achieve the experiments is LEO 1430 scanning electron microscope. All the experiments are executed in room temperature and fixed 5*10 - 5 mbar of pressure and 29 mm of working distance with an average 35%of humidity. A mathematical description of the charging process and trapped charge is presented. Although this modulation is based on simple ideas of the electricity, it has a good agreement with experimental results. Different types of biological samples have been used to investigate mirror effect phenomenon occurs in these samples. Where in some samples mirror effect appears significantly while there is no sign of charge trapping in other samples. The main sample that has been adopted for exploring the phenomenon is Rotula, which belongs to the buccal apparatus of sea urchin, due to its relatively large area and thickness in comparison to other available specimens and the stability of the mirror effect. Different locations are chosen in each side of Rotula to compare among them. As the most effective parameter in this phenomenon is charge trapping and detrapping, the charge accumulation on the sample surface is measured by two different methods namely magnification factor method (MFM) and disappearing method (DM). The MFM is used to investigate five different locations of the inhomogeneous oral side, while the homogeneous aboral side is used to compare between the values of the trapped charge using MFM and DM. The dielectric constant of the aboral side of Rotula has been calculated by making use of the theoretical aspects of MFM and DM.
