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دراسة الميكروبية والتداخل البكتيري للفطريات المصاحبة لالتهابات الانف والاذن لمرضى السكري وابيضاض الدم == Microbial Study and Bacterial Interaction with Mycosis Associated with Nose and Ear Inflammatory of Diabetes and Leukemia Patients
Author name:
علیاء فخري خلف البیاتي
Supervisor name:
امال عباس محمد الفخري
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Baghdad - College Of Science For Girls
Language:
Arabic
University location:
Baghdad
First pages:
24T3392 - p.pdf
Abstract:
One hundred and ten clinical samples were collected from hospitalized patients in hematology - oncology department in Baghdad Teaching Hospital and Al - Kindy Teaching Hospital, during the period from November 2014 to June 2015.The samples included 48 female and 62 male patients who are suffering from diabetes mellitus (DM) and leukemia. The ages of patients ranged between 13 - 82 years. The swab samples taken from the nose and ear of patients were (67 with diabetes mellitus and 43 with leukemia). The results of fungal culturing revealed that 50 samples of DM patients (74.63%) and 31 samples of leukemia (72.09%) were diagnosed as positive ones.From the collected clinical samples that gave positive results; it was found that Candida spp. was the most common fungi isolated from ear swabs (50%) followed by Aspergillus spp. (42.86%) and Penicillum spp. (7.14%).The results of fungal (molds) species isolated from the nose of DM patients revealed highly significant differences between fungal rates, Aspergillus spp. were isolated with highest levels (48.15%), followed by Penicillum spp. (37.04%); however, Rhizopus spp. was isolated with low levels (14.81%). On the other hand, it was found that Candida spp. and Rhodotorula spp. were the most yeasts isolated from the nose of DM patients. They were isolated with rates as (45.83%) and (41.67%) respectively. Nevertheless, the genus Cryptococcus spp. was isolated with low levels (12.50%). The results also revealed that males were more susceptible to fungal infection compared to females. Moreover, it was found that the fungal are more prevalent in ages over fifty and ages between thirty one and fifty years compared with younger ages between ten and thirty years old. From the collected clinical samples of leukemia patients, highly significant differences were found between the rates of fungal infection. The level of Aspergillus spp. was (90.91%) and Rhizopus spp. (9.09%), while the levels of yeasts were (88.24%) and (11.76%) for Cryptococcus spp. and Candida spp. respectively.The frequent species of Aspergillus identified included A. niger (37.78%), followed by A. flavus (35.56%), A. fumigatus (17.77%) and A. terreus with lowest levels (8.89%). The results also revealed that males are more susceptible to fungal infection compared to females. Moreover, it was found that the fungi are more prevalent in ages 31 - 50, followed by younger ages (10 - 30) compared to old ages, over fifty years old.All fungal isolates were identified according to macroscopic and microscopic characteristics in addition to biochemical tests for yeast identification. Vitek 2 system is another confirmatory test which was carried out for further characterization for the yeast species. The results of Vitek 2 system revealed that Candida spp. were isolated with the highest level (41.67%), followed by Cryptococcus spp. (37.5%) however, Rhodotorula spp. was isolated with a low level (20.83%). A confirmatory test for the selected isolates of A. fumigatus using polymerase chain reaction (PCR) analysis technique was also conducted. This test has been done by using primers specific for hemolysin gene (aspHS). The results of the molecular method were closely related to those obtained from cultural methods with exception of one isolate which was positive by PCR method nevertheless, it was negative by culture method. The results of PCR analysis technique, revealed that 6 isolates out of total 8 (75%) were positive for the presence of aspHS gene compared to culture methods which showed 5 isolates out of total 8 (62.5%) had the ability to induce hemolysis when grown on blood agar .The inhibitory effect of Bacillus subtilis against Aspergillus spp. was also conducted, the results indicated that B. subtilis isolate has antimicrobial activity against Aspergillus spp; the diameter of inhibition fungal growth were 39.3 ± 01.5, 48.6 ± 02.2, 40.5 ± 02.8 and 43.5 ± 01.7 mm for A. fumigates, A. flavus, A. niger and A. terreus respectively compared with the diameter of controls which were 80.0 ± 05.3, 78.0 ± 03.9, 43.5 ± 01.7 and 72. ± 02.6 mm respectively. The radial inhibition growth of Aspergillus spp. treated with B. subtilis were 51%, 38%, 47% and 44% for A. fumigatus, A. flavus, A. niger and A. terreus respectively compared with treatment of control.
