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دراسة مقارنة تاثير عقاري كلابنكلامايد والميتفورمين على فعالية اراجدونيت 15 لايبوكسيجينيز واكسدة الدهون الفوقية في امصال بعض المرضى العراقين المصابين بالسكري النوع الثاني
Author name:
حيدر خابط عبود القيسي
Supervisor name:
محمود حسين هدوان
General topic:
Chemistry
Specific topic:
Chemistry
Degree:
Master
University:
University of Babylon - College Of Science
Language:
English
University location:
Babylon
Abstract:
Diabetes mellitus is a group of metabolic disorders described with chronic hyperglycemia arising from disorders in insulin secretion, insulin action or both. The presence of these consequences in chronic hyperglycemia led to damage, dysfunction, and failure of some organs, principally eyes, kidneys, heart, nerves, and blood vessels. | Enzymatic lipid oxidation comprises an enzyme as a catalyst, and provides very specific stereo and regiospecific compounds. Non-enzymatic does not produce specific compounds, many stereo-and positional isomers produced. There are three families of enzymes implicated in the oxidative metabolism of arachidonic acid. These involve the lipoxygenases, which form leukotrienes (LT), hydroperoxyeicosatetraenoic acids (HPETEs), hydroxyeicosatetraenoic acids (HETEs), and hydroxyoctadecadienoic acids (HODEs). | The aims of the study are : | - Determining the effects of glibenclamide and metformin on the following parameters : lipid profile, lipid peroxidation, total antioxidant capacity, α-tocopherol, total oxidant status and arachidonate 15-lipoxygenase activity | - Developing a new precise method for assessment catalase activity and compared it with ordinary methods. | The study included a hundred and twenty patients with diabetes type II aged between (44.5±9.2) years old models were obtained from patients in a clinical study in Al-Najaf Center for Diabetes and Endocrinology (Al-Najaf City, Iraq) additionally to sixty individuals representing a healthy control group aged between (42.4±7.2) years old within the period of March 2015 to the end of December 2015. | ABSTRACT | ii | The study includes the following groups : | 1- Patients with diabetes type II (30males & 30 females) were treated with metformin (Glucophage). | 2- Patients with diabetes type II (30males & 30 females) were treated with (Daonil) Glibeneclamide. | 3- Healthy subjects (30males & 30 females) were selected as a control. | Subjects have been diagnosed with diabetes, according to World Health Organization (WHO) standards, which include that all patients within the study does not suffer any of the cases high blood pressure, not smoking, not abusing alcohol or any treatment. All cases outside of these criteria were excluded from the study, the use of serums and blood as models for the study were obtained on serums from the blood of patients and healthy controls to be measured levels of both glucose blood in the case of fasting cholesterol , triglyceride and lipoproteins (HDL-c, LDL-c, VLDL-c,) as well as the measurement of hemoglobin level glycated HbA1c in the blood and so were measured concentration malondialdehyde MDA as an indicator of lipid peroxidation way spectrofluorometer , total reactive oxygene species as an indicator total oxidant status, also have been measured total antioxidants and measuring the concentration of vitamin E, the development of a new way to measure the effectiveness of an catalase enzyme through add correction factors to the equation first Order Reactions to increase accuracy and precision , measure the levels of enzyme Arachidonate 15-lipoxygenase , it was shown that there were significant differences and other variables were not significant for all the different types of diabetes-related treatment. It was found by studying the effectiveness of the enzyme 15- lipoxygenase not changed with the male patients treated with mtformin and glibenclamide compared with healthy | ABSTRACT | iii | controls, but there were significant decrease in the efficiency with female therapists with glipenclamide and mtformin there is a significant increase in the concentration of MDA in each of the male and females treated with metformin and glibenclamide in contrast, a decrease in the ability of antioxidants in both males and females treated with glibenclamide but this ability does not change with both male and female therapists patients with metformin . | Conclusion | oxidation of lipids enzymatic and non enzymatic leads to the formation of lipid peroxides that have a direct impact on the diabetes and that the lipoxygenase enzyme the source of enzymatic sources that reduce antioxidants and increase the concentration of free radicals. glibenclamide reduces concentration of the enzyme as well as increase antioxidants capacity and reduces lipid peroxides and reduce HbA1C In addition Show that the patients were treated with metformin or glibenclamide had non-significantly changed in serum α-tocopherol levels than the control group. | The results show decrease HDL levels; when compared with healthy subjects, but incresed LDL, VLDL, TC, and TG ; that is may be related to increased oxidative stress.
