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Immunological Status Of Seminal Plasma For Bodybuilders On Androgenic Anabolic Steroid Hormones == Immunological Status Of Seminal Plasma For Bodybuilders On Androgenic Anabolic Steroid Hormones
Author name:
Anwar M. Rasheed Abdulhussein
Supervisor name:
Dr.Abduladheem Y. Abbood AL.Barra
General topic:
Medicine
Specific topic:
Medicine
Degree:
Master
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
First pages:
19T1133 - p.pdf
Abstract:
Exercises done by bodybuilder have great benefit for the general health and muscle size and performance. Anabolic androgenic steroid hormones are commonly used by bodybuilders to increase muscle mass and strength depending on its mode of action to increase the level of nitrogen retention in muscle, insulin like growth factor IGF - 1 increase in muscle tissue and seminal fluid. IGF - 1 responsible for Cell proliferation, growth and inhibiting apoptosis activity. Also AAS generally has an immunmodulatory impact on cytokines interactions as well as infertility. This study was designed to estimate the changes in cytokines in seminal plasma, sperm count in the seminal fluid and estimation of testosterone concentration in serum due to the impact of androgenic anabolic steroids hormones in bodybuildrs. Eighty male volunteers their age groups are ranging from 21 - 35 years. They were divided into three groups; the first group 27 age matched volunteers apparently healthy (healthy control group) , Second group 27 bodybuilders players attending the sport club with androgen anabolic steroid hormone abuse (AAS) (steroid group), and the third group 26 bodybuilder players attending this sport club in Baghdad without AAS uptake (fitness group ). A blood sample and seminal fluid were obtained from each individual involved in this study. Blood, serum and seminal plasma were prepared from these samples were obtained and applied for estimation of testosterone level by using enzyme immuno - florescent technology. Anti - sperm Ab test by heamo - aglutenation method was used to exclude the positive cases from this study.Heparinized blood was used for estimation of neutrophil phagocytic activity Sperm count was done for each seminal fluid sample, cytokines were estimated by sandwich ELISA method, in seminal plasma, these cytokines are IL - 6, IL - 10,IL17a,TNF - ? and INF - ?. The bodybuilders with AAS uptake showed a significant reduction in sperm count (P=0.00), fitness group sperm count showed slight nonsignificant decrease compared to healthy control group (P=0.7). Serum testosterone level in the AAS uptake group showed a significant decline compared with fitness and healthy control groups (P= 0.00). Exercise alone increases the level of IL - 6 (P=0.00), while exercise with AAS uptake decreases the level of IL - 6 (P=0.06). The fitness group showed highly significant increases in TNF alpha (P =0. 00). IL - 10 as an anti - inflammatory cytokine shows a high level in the AAS uptake group compared to the healthy group (0.04). IL - 17 as an inflammatory cytokine showed high significant increase in steroid group and fitness group (P=0.00), which indicate that both exercises with and without AAS uptake increases the level of IL - 17 secretion(P= 0.02) separately or in combination. Phagocytosis of AAS uptake group showed a highly significant decline (P= 0.00).The correlation results showed that there is a complex network interactions between the studied cytokines. The Phagocytic activity of the AAS uptake group showed a strong correlation with both IL - 6 and IL - 10 levels. IL - 10 as a suppresser cytokine has a significant correlation with TNF - alpha, INF - gamma and IL - 17A while, IL - 10 showed a negative correlation with IL - 6. IL - 17 which act as an inflammatory cytokine has a significant correlation with all cytokines except IL6 and phagocytic activity. Generally seminal plasma cytokine network may be under the effect of immune regulation mechanism and cytokine inactions as well as, the impact of steroids uptakes. In general the final impact of AAS uptake on bodybuilders immunity are declining in phagocytosis and an increase in IL - 10 which act as suppressor to the Th1 immune response and increase of IL - 17 which has a role in increasing the inflammatory response so, these results suggest that AAS abuse players may be more liable for infection and decrease in sperm count which may cause temporary or permanent infertility.
