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دراسة العوامل الكيميوحيوية والتنميط الجيني للانزيم المحول للانجيوسين لدى مرضى اعتلال الكلية السكري النوع 2 == Biochemical Study and Angiotensin converting enzyme polymorphism of Nephropathy in T2DM Patients
Author name:
زينب عباس جواد الطالبي
Supervisor name:
حيدر كامل زيدان السعدي | محمود حسين هدوان
General topic:
Chemistry
Specific topic:
Chemistry
Degree:
Master
University:
University of Babylon - College Of Science
Language:
English
University location:
Babylon
First pages:
25T1553 - p.pdf
Abstract:
Diabetes is characterized by high levels of blood - glucose resulting from defect insulin hormone secretion or insulin action, or both of them. Chronic high blood - sugar lead to development and failure in many organs in the body such as heart, blood vessels of the eyes, nerves system and kidneys.The Study evaluation of Angiotensin Converting Enzyme - 1 and Angiotensinogen in Type 2 Diabetic patients according to the following steps : it is directed to evaluate Biochemical parameters in type 2 diabetic patients. Measure the levels of some hormones and some parameters. Study the relationships between these hormones and the Biochemical parameters. The association of Angiotensin converting enzyme gene in some hormonal and physiological parameters in type 2 diabetic Iraqi patients. Determined by Polymerase Chain Reaction (PCR). while the hormones including (Insulin and Erythropoietin) and the physiological parameters (insulin resistance, insulin sensitivity, Fasting Blood Glucose; HbA1C; microalbuminuria; systolic and diastolic blood pressure).This case - control study was done in a period of March 2016 to May 2016, from Al - Sadder Medical City in Al - Najaf center for Diabetes and Endocrine. All blood samples of patients were collected in fasting (8 - 10hr.) Number of patients in Diabetes mellitus (DM) type II. Total patients were (n=66), then divided into three groups according to the duration of the disease, the First subgroup (less than 5 years) (n=22), Second subgroup (5 - 10 years) (n=22) and the last subgroup more than 10 years (n=22). The healthy control includes (n=22) subjects, total numbers becomes (88 persons).VISummaryExcluded from this study : Type I diabetes, Females, smokers Patients, random Patient uncontrolled hypertension, alcoholic patients, types of cancer, Hepatitis and Removal of glands. Both groups were subjected to full history including age, BMI, complete clinical examination such as blood pressure measurement. Laboratory measurements including, fasting blood sugar, HbA1C, Microalbuminuria, Insulin hormone, Erythropoietin hormone, Estimation insulinresistance and sensitivity, Extraction DNA from frozen blood for all control and patients then, determination of ACE I/D genotype were done by using PCR technique.The results show significantly Elevation in Microalbuminuria, HbA1c , Fasting blood - sugar, and insulin resistance in nephropathy diabetic type 2 patients compared with healthy control.ACE - 1 enzyme levels have significant increase and lead to the hypertension in the Diabetic patients. There is no relationship between Erythropoietin hormone level and blood pressure deduce that EPO did not raise blood pressure with the patients according to the results of this study, but caused from Angiotensin converting enzyme - 1. The frequency of DD genotype was 21/44 (50%) in patients type 2 more frequent and significant that was significantly higher than that of patient group ID and II with P value =0.036. while there was no significant in ID genotype between patients in group ID 14/44(31%) with p=0.92 and patient in group II genotype 9/44 (20%) with P value=0.07.This study found that DD genotype of ACE gene may be associated with development of diabetic nephropathy among Iraqi patients and this could lead to the prevention and treatment of this complication (Diabetic nephropathy) in Iraqi diabetic patients.
