دراسة للطفيليات المعوية المعزولة من الخضروات المطرية من بعض اسواق مدينة بغداد
Author name:
وسن عداي مهدي المرسوي
General topic:
Biology
Specific topic:
Microbiology - Fungi
Degree:
Master
University:
University of Baghdad - Ibn Al-Haytham College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Baghdad
First pages:
24T3523 - p.pdf
Abstract:
A study was carried out during the period from November 2010 to October 2011 to detect the intestinal parasites which contaminate the common fresh vegetables. A total of 303 samples were taken from three wholesale markets and three retail markets, in Baghdad province. Ten kinds common vegetables were selected. These vegetables were Celery (Apium graveolens); Parsely (Patroselinum crispum); Garden cress (Lepidium sativum); Leek (Allium porrum); Radish (Raphanus sativus); Lettuce (Lactuca sativa); Spinach (Ipomoea aquatica); Beet (Beta vulgaris); Mint(Mentha spicata) and Cabbage (Brassica oleracea).Sedimentation and floatation techniques were used for samples examination. Three different solutions (ZnSO4, saturated NaCl , saturated sucrose ) were used in floatation technique. The present study has been shown that the collected samples of vegetables were contaminated with sixteen species of intestinal parasites. It was found that the percentages of contamination with these parasites were as following : (Giardia lamblia) (29.4%), Entamoeba histolytica(24.5%), Entamoeba coli(16.9%), Balantidium coli (8%), coccidia(9.9%) and Blastocystis hominis(0.7%). For intestinal helminthes Ascaris lumbricoides (15.6%), Toxocara spp.(10.3%), Fasciola spp. (5.3%), Schistosoma haematobium (1.7%), Schistosoma masnsoni (1.4%), Taenia spp. (4.7%), Diphyllidum caninum (3.9%), Trichuris trichiura (3.3%), Enterobius vermicularis (6.9%) and Nematoda larvae (6.3%).The present study revealed that the percentage of contaminated vegetables by using sedimentation technique (72.3%) was significantly higher than that of flotation techniques (53.1%). Additionally it was found that, the different kinds of vegetables showed a significant difference in the percentages of contamination with the parasites in both techniques. Thus, the percentage of contaminated Garden cress was (93.5%), (72.4%) in both sedimentation and flotation technique respectively, while Cabbage showed (50%), (36.7%) respectively.A significant high percentage of contamination was recorded in Al - Nahrin wholesale market (89.2%) in comparison with low percentage (59.4%) in Al - Rasheed wholesale market. Additionally, it was found that the percentages of contaminated vegetables in wholesale (76.26%) were significantly higher from that of retail market (64.76%).The present study, also, showed that the percentage of detection of parasites by using ZnSo4 solution (31.2%) was more efficient than that showed by saturated sucrose (8.9%). Additionally; the soaking with normal saline (47.83%) showed a higher percentage of contamination than that by using distilled water (17.39%).The percentages of seasonal contamination were varied significantly, therefore, it was found that spring showed the highest percentage (29.7%) while summer the lowest (20.9%). The washed samples of Garden cress which obtained from the three wholesale markets, reduced the percentages of contamination with parasites but did not remove the parasites completely. Therefore, a significant difference has been noticed between washed (56.76%) and unwashed samples (76.66%).Concerning, the percentages of infections with intestinal parasites, it was found that the medical center (14 July) showed (29.4%) while Al - Ameria center (19.2%). Furthermore, the percentage of parasites infections was significantly higher in the summer (46%) than in winter (12.67%).The present study indicated that there were a positive coordination between the high percentages of contaminated vegetables and the high percentages of infection in the medical centers as in the case of G.lamblia and E.histolytica.Finally, it could be conducted that the contaminated vegetables played an important role in transmission of intestinal parasites to human, particularly, when these vegetables were eaten fresh without sterilization or good washing.
