تعدد النمط الوراثي للجين GSTT1,GSTM1 في الاطفال العراقيين المصابين بمرض السكري النوع الاول == Genetic Polymorphism of (GSTM1, GSTT1) Gene in A sample of Diabetes Mellitus type1 in Iraqi children
Author name:
سهام مجيد محمد
Supervisor name:
بتول علي شهاب | رافد علاء جاسم
General topic:
Biology
Specific topic:
zoology - Genetics
Degree:
Master
University:
University of Baghdad - College Of Science For Girls
Language:
English
University location:
Baghdad
First pages:
24T3487 - p.pdf
Abstract:
Type1 diabetes mellitus uncommon problem in Iraq which increase incidence of effected young age groups ,and its regards one of the multifactorial disorders with genetics and environmental factors playing important role in its cause and pathogenesis ,complication, prognosis.In diabetes, the defects in cellular metabolism especially hyperglycemia results in increasing free radicals. These radicals react with other vital cellular molecules which are responsible in diabetes side effects. Human glutathione S - transferees (GST) are a family of enzymes that catalysis conjugation of electrophilic substances with glutathione. In this research the deletion and expression of two of the most important genes of this family; GSTT1 and GSTM1 genes was investigated as the risk factor for type1 DM and control of the disease glycated hemoglobin (HbA1c), body mass index (BMI) as ( indicators of diabetic control), in relationship to the age , gender , age of the onset, duration of the disease, and also study of 10 parents of type1 DM to demonstrate the genetic role of GSTs genes in the disease.Forty four type1 DM patients were enrolled (23M & 21 F) and compared with 42 non diabetic counterparts matching with age and gender as regards as control group.Deletion of GSTM1 and GSTT1 genes was observed in (3) diabetics’ patients as compared with control group 3/44 (6.8%), 0/42 (0%) respectively . Also this percentage was increased, 8/11 (72.73) 1/11 (9.09) in the poor control type1 DM (HbA1c < 10), while in the underweight 5/10 (50%), 6/10 (60%) are Significant Correlation between GSTM1 null / GSTT1 present genotype, also study showed more incidence in the male than female patients.There are increase incidence of the expression and deletion of GST genes with aging, chronicity of disease, glycemic control, family GST gene states , and BMI.The type1 DM had effect on the GST states according to the gender, age , age of onset and duration of the disease .
