دراسة تاثير المخلفات النباتية والحيوانية والفطر الاحيائي Trichoderma viride في مكافحة مرض تعفن جذور الباميا المتسبب عن الفطر Macrophomina phaseolina == A study of effect of plant and animal residues and the bioagent Trichoderma viride in controlling okra root rot disease caused by Macrophomina phaseolina
Author name:
نور مهدي صالح المالكي
Supervisor name:
ضياء سالم علي الوائلي
General topic:
Agricultural sciences
Specific topic:
Plant - Prevention
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture - Department Of Plant Protection
Language:
Arabic
University location:
Basrah
First pages:
31T1876 - p.pdf
Abstract:
This study aimed to use plant and animal residues and bioagent Trichoderma viride against pathogenic fungus Macrophomina phaseolina which caused okra root rot disease in pots and field and to detect the active compounds in okra leaves by GCMS technique.Three pathogenic fungi were isolated from infected plants, they were M.phaseolina , Rhizoctonia solani and Fusarium solani .The fungus M.phaseolina gave the highest percentage of occurance and freguencywhich were 55 and 66.67% .The pathogenicity test showed that M.phaseolina led to decrease germination pereen and increase damping - off up to 20 and 26.67% respectively, compared to control treatment which was 80 and 0% respectively.The results explained that the animal manure (F) , rice (R) and wheat (w) led to increase the germination percent up to 66.67% , 60 and 53.3% respectively compared to control treatment which was 40% , the fresh weight also increased in the same treatments up to 1.36 , 1.25 and 1.28 gm respectively compared to control which was 0.64 gm .It was founed the less infection intensity with M.phaseolina in pots was noticed in the treatment RTv (bioagent +rice) and FTv (bioagent+animal manure), it was 0% compared to control treatment which was 90%, which the highest shoot weight and plant height were noticed in the treatment FTv which were 6.22 gm and 32.03 cmrespectively compared to control which were 1.33 gm and 22.55 cm. The field experiment results revealed that the less infection intensity was observed in the treatment FTv which was 0% compared to control which was 62.22% .The treatment RTv gave a high shoot weight which was 156.11 gm compared to control which was 46.39 gm, while the high plant height was obsrved in the treatments RTv and FTv which were 73.9 and 71.98 gm respectively compared to control which was 40.33 gm. It was also founed that the high plant production was noticed in the treatment RTv and FTv which were 64.53 and 63.10 gm respectively compared to contorl which was 1.44 gm.From the other hand ,the total chlorophyl was noticed in the treatment RTv ,R,FTv,wTv and F which were 1728,224 ,1629.96,1454.442,1377.884 and 1281.168 mgm/100gm fresh weight respectively compared to 359.9 mgm/100gm fresh weight. The results of phenotic compouneds detection in the plant leaves by using GCMS technique revealed a presence of some compouneds such as Pentanol, 1,2,4 Triazole and Cyclohexanol with the treatments RTv,FTv and wTv ,these compouneds were not been recorded in the other treatments.
