علاقة تعدد الاشكال الجيني للهرمون المطلق لهرمون النمو و مرض ضخامة الاطراف للمرضى في العراق == The Association of Growth Hormone Releasing Hormone Gene Polymorphism with Acromegaly in Patients from Iraq
Author name:
ذو الفقار غالب تركي
Supervisor name:
ناظم مشتاق هاشم
General topic:
Biology
Specific topic:
Biotechnologies
Degree:
Master
University:
Al-Qasim Green University - College of Biotechnology - Department of Medical Biotechnology
Language:
English
University location:
Babylon
Key words:
- Growth Hormone Releasing Hormone Gene
- DNA Mutation
- Endocrine
- Acromegaly
- Growth Hormone
Abstract:
Acromegaly is a rare syndrome categorized by extreme excretion of growth hormone via a pituitary adenoma, happening everywhere in the world also in Iraqi population. Practically all conditions of acromegaly are produced by a benign tumor in the pituitary that creates much growth hormone. In additions, some tumors in the body may yield an element known as growth hormone releasing hormone which lead to stimulating the pituitary for over secretion of growth hormone. The main objective of study was to determine the association of GH,IGF-1,GHRH,FBS biochemical parameter as well as GHRH gene polymorphism with the severity of acromegaly disorder. The biochemical and molecular genetic study is performed from August 2022 to October 2022, including 80 samples of Iraqi ACM patients that collected from Diabetic National Center in AL-Mustansiriyah University and 40 samples from healthy . Biochemical study includes 80 samples ACM patients and 40 were as a control. The measuring of FBS by Trinder method , GH, IGF-1 were achieved by using sandwich chemiluminescence immunoassay technique and The measuring of GHRH was achieved by using the enzyme-linked immunosorbent assay (ELISA) kit to estimate the variations among these biochemical parameters in healthy and ACM patients. The genetic material (DNA) was isolated from ACM patients and healthy controls. The one fragment of GHRH gene (amplicon size 791 included exon 2,3 and intron 2) within chromosome 20 was amplified via polymerase chain reaction technique.
