دراسة نسيجيه مرضيه لسمية الدايوكسين في ذكور الجرذان المختبرية البيضاء Rattus norvegicus == Histopathological study of dioxin toxicity in male rats Rattus norvegicus
Author name:
ياسمين جاسم محمد
Supervisor name:
زینب وحيد خضير | صالح كاظم مجید
General topic:
Medicine
Specific topic:
Diseases
Degree:
Master
University:
University Of Basrah - Faculty Of Medicine
Language:
English
University location:
Basrah
First pages:
19T1232 - p.pdf
Abstract:
In the present study is the toxicological and pathological effects of dioxin on rats behavior (Rattus norvegicus), and the effects of dioxin on weight before and after administration were studied. Thirty two male rats (Rattus norvegicus) were used, the animals were divided into four equal groups, each all of which were administered orally : olive oil (1 ml), 50 ng, 100ng and 200 ng per animal of dioxin respectively for 70 days. On estimation of body weight of animals, the results showed significant decrease of animal weight when compared with the control group (P≤ 0.05). At the end of this treatment period, animals of each group were sacrificed after anesthesia using chloroform inhalation. The result showed variable gross changes in some internal organs (liver, kidney and spleen) in treated groups in comparison with the control group. The study included significant changes in the histopathological structure of many organs due to the effect of dioxin ( liver, kidney, spleen, stomach and skin) in comparison with the control group.The liver showed some important changes like degeneration, necrosis of hepatocytes and sever fibrosis of periportal area. In the spleen there is decrease in size of spleen and atrophy of lymphocyte of whit pulp. And in the kidney there are some changes include degeneration of proximal convoluted tubules and glomeruli atrophy.In stomach the important changes was revel include erosion of mucosa of glandular region and hyperplasia with hyperkeratosis of non glandular region.And in the skin appeared some critical changes like atrophy of hair follicles, sebaceous gland and thinning of epidermis.It was concluded that dioxin has a significant toxic effect on laboratory animals (rats), confirmed by its histopathological effects on internal organs and skin of exposed animals.
