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التحري الجزيئي عن جين الضراوة rmp لبكتيريا Neisseria gonorrhoeae المعزولة محليا من المرضى البالغين == Molecular Detection of Virulance rmp Gene in Neisseria gonorrhoeae Isolated from Adult patients

اسم المؤلف: مهـند كـريم عنيد الساعدي

اسم المشرف: نورية عبد الحسين علي

الموضوع العام: علوم الحياة

السنة: 2013

الموضوع الدقيق: التقنيات الاحيائية

الدرجة: ماجستير

الجامعة: جامعة بغداد

اللغة: الانكليزية

مكان الجامعة: بغداد

الصفحات الاولى: 📎 24T3158 - p.pdf

المستخلص: This study included 80 clinical samples from patients suspected to have gonorrhoea (a urine sample and two direct smears from each patient) from different areas of Baghdad, the Kut and Suwairah regions, from private clinics and laboratories for the time period from November 2011 till June 2012. Cases caused by gonococci were diagnosed by finding the Neisseria gonorrhoeae bacteria in the samples using microscopic examination and culturing on media cultures for all the 80 samples which included 17 (21.25 %) women and 63 (78.75 %) men. Microscopy and culture revealed that 47 out of the 80 (58.75 %) samples were positive : 35(43.75 %) of them diagnosed by both microscopy and culture on chocolate agar, Neisseria media and modified Thayer - Martin media; while 9 (11.25 %) samples were diagnosed by microscopy alone (cultures were negative) and the remaining 3 (3.75 %) samples by culture alone (microscopy was negative). Results of the genetic diagnosis looking for the bacteria causing gonorrhoea using primers specific for the rmp gene which were specially designed for this study revealed that 77 (96.25 %) of the direct smears and 75 (93.75 %) of the urine samples were positive. Results of the Nitrogen bases sequence of the PCR technology of the samples in this study revealed consistency reaching up to 99 % with the Nitrogen bases sequence of the rmp gene present in the N. gonorrhoeae strain of the WHO. This study presented high specificity and sensitivity for the diagnosis of gonorrhoea using the PCR technology which is cheaper and faster than the conventional methods currently used in the hospitals and laboratories.