عزل وتشخيص البكتريا المرافقة لبعض الاصابات المرضية في بعض الاسماك في محافظة البصرة، العراق == Isolation and diagnosis of bacteria associated with some disease infections in some fishes in Basrah Governorate, Iraq
Author name:
نادية علي حسين الشمري
Supervisor name:
نجم رجب خمیس | اسعد محمد رضا الطائي
General topic:
Agricultural sciences
Specific topic:
Zoology - Fish And Marine Wealth
Degree:
Master
University:
University Of Basrah - Faculty Of Agriculture - Department Of Fish And Marine Wealth
Language:
Arabic
University location:
Basrah
First pages:
31T1871 - p.pdf
Abstract:
The aim of this study was to isolate and identifying the pathogenic and nonpathogenic bacteria from infected fish, healthy fish and water. One hundred andfifty three healthy and infected fish were collected during the period from October2015 till April 2016, which included seven species viz. : (6) Acanthopogrusarabicus Iwatsuki, 2013; (65) Cyprinus carpio L., 1758; (14) Coptodon zillii(Gervais, 1848); (15) Hypophthalmichys molitrix (Val., 1844); (23) Planilizasubviridis (Val., 1836); (15) Oreochromis niloticus (L., 1758) and (15) Poecilialatipinna (Lesueur, 1821).The samples of water and fishes were collected from seven different stations inBasrah governorate as, Hartha station (Floating cages), Al - Mashab river station,Basrah University station (Terrestrial ponds), Al - Seeba station (Terrestrial ponds),Al - Marbad station (Concrete ponds), Mehaijran station (Terrestrial ponds) andShatt Al - Basrah canal.The environmental properties of water have studied such as pH, the lower valuewas 6.9 in Al - Mashab river station, while the maximum was 8.4 in Al - Seebastation, meanwhile the maximum average was 7.8 in the Shatt Al - Basrah canal. For salinity the range was recorded from 1.5 ppt at Al - Mashab river station and 45.6 ppt at Shatt Al - Basrah canal. Otherwise the maximum value was 35.2 ppt in the same station.The values of temperature were fluctuated from 12 ᵒC in Al - Marbad station to 31.2 ᵒC in the Shatt Al - Basrah canal.The results of the present study appeared that, the fish was infected with different diseases, including : Spring Viraemia of Carp Virus, Viral Hemorrhagic Septicemia, Bacterial Gill disease, Fin rot, Vibriosis, Dropsy, red mouth and Pox disease.The bacteria were identified in the present study using Vitek II system, API kits and biochemical tests. The accurate identification of Vitek II was between 85 - 99% for most bacterial genera, while failing to identify the species of Vibrio.Many bacterial species were identified from infected fish, from Spring Viraemia of Carp Virus disease, five species were identified as Staphylococcus lentus, Aeromonas hydrophila, Vibrio cholerae, Klebsiella pneumoniae pneumoniae and Burkholderia pseudomallei. From Vibrosis disease, seven species were identified as Staphylococcus haemolyticus, V. cholerae, V. alginolyticus, V. costicola, Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa. From Dropsy disease only one species was identified as Raoultella ornithinolytica. From red mouth disease, three species were identified as Enterobacter cloacae complex, Shigella group and V. cholerae. From Fin rot disease, two species were identified as Aeromonas hydrophila and Aer. sobria. From Diplostomatosis, also two species were identified as V. diazotrophicus and Aer. hydrophila, while from Pox disease, only two species were identified as V. furnissii and Globicatella sanguinis. Finally from Ascocotyle only E. coli was identified.On the other hand, the bacteria were isolated from the water of studying stations and identified as : Staph. lentus, Aer. hydrophila, R. ornithinolytica, V. Alginolyticus, V. furnissii, E.coli, V. costicola , V. gazogenes , B. pseudomallei, V. cholerae , Ent. cloacae¤Aer.sobria, K. pneumniae pneumniae, G. sanguins, E. areogenes, P. aeruginosa.Meanwhile, the bacteria also isolated from healthy fishes and identified as : Aer.hydrophila, E. coli, V. alginolylius, B. pseudomallei, V. gazogenes, P. aeruginosa,Ent. cloacae, K. pneumniae pneumniae and Proteus vulgaris.
