استخلاص وعزل بعض المركبات الفعالة بايولوجيا من جمار النخيل Phoenix dactylifera == Extraction and Isolation Some Active Biological Compounds From (Phoenix dactylifera)
Author name:
وصال عبد الرحمن سالم الثامري
Supervisor name:
اقبال جاسم بدر | هناء كاظم موسى
General topic:
Chemistry
Specific topic:
Chemistry
Degree:
Master
University:
University Of Basrah - College Of Science - Chemistry Department
Language:
Arabic
University location:
Basrah
First pages:
25T1605 - p.pdf
Abstract:
It is notable that Iraq characterized by the availability of palms trees , according to 2012 statistics it has been considered as the fifth largest country in production of dates in the world (Wikipedia), and because of the rareness study about the palm it almost non - exist in Iraq , therefore it has been proposed this study in order to find out the medical uses of the Jummar .The study has included preparation of five extracts which they are aqueous ,70% alcoholic , glycosidic , alkolids and flavonoids .In addition , the isolated oil and makes some chemical and physical detections such as solubility.Moreover , it included knowledge of content for this extracts and identify the number of components in each extract and diagnose the chemical identity using several techniques such as "Thin layer and Colum" chromatography ," FTIR , U.V - Visible" spectroscopy.It has also been studied the effect of the glycosidic , alkolids , flavonoids and oil extracts on the level of blood sugar in normal rabbits and the rabbits suffering from hyperglycemia which induced by alloxan ,in addition to the effect of the pill lower the blood sugar and the mixture from the pill with alkloidic extract , beside the mixture of the pill with oil extracts.Furthermore, the test giving a potion (dosage - dose) of 500mg/Kg which is had reduced the blood glucose level for 24h with significant difference p> 0.001 , the isolated oil was less efficient with significant difference p> 0.001 .The cytotoxicity test was also conducted by using In vivo method on human blood does not affected by isolated compounds from Jummar and oil isolated oil , and it safe and non - toxic.The effect of above extracts was studied on the growth of some kind of positive Gram Staphylococcus aureus , and negative Gram bacteria Aeromonas hydrophila using Agar diffusion method , the flavonoid extract showed the high test 16mm inhibition zone compared with alkaloid extract which showed 12mm inhibition zone for negative Gram bacteria Aeromonas hydrophila.The effectiveness of isolated compounds and oil isolated were estimated as antioxidants and compare them with Butyrated hydroxyl toluene (BHT) which appeared that flavonoids extract has a higher effective antioxidant.The interference of isolated compounds from palms jummar and isolated oil with DNA has been studied by tracking the change in the concentration of the isolated compounds after mixing with DNA in the various time and by tracking the change of the UV spectroscopy. Finally , it has been also tracking the change of the concentration of DNA by using the Electrophoresis Technology and UV photographic technology and both Technologies showed the interference between the isolated compounds and the DNA.Also the glycosidic extracts was studied on the growing of cancer cells Rhabdomysarcoma , and seemed clear inhibition at concentration (10mg/ml) in (72) hours and inhibition ratio was about (37.26%).
