معدل عدوى الكلاميديا كسبب في الولادة المبكرة باستخدام طريقة اختبار سلسلة تفاعل البلمرة مقابل اختبار انزيم المناعية وتاثيره على نتيجة ولادة الجنين == The Rate of Chlamydia Infection as a Cause of Preterm Labour (Using PCR, Elisa) and effect on fetal birth out come
Author name:
بان كاظم عبد الرحيم
Supervisor name:
وسن وجدي ابراهيم
General topic:
Medicine
Specific topic:
Obstetrics and Gynecology
Degree:
Higher Diploma
University:
University of Baghdad - Faculty Of Medicine
Language:
English
University location:
Baghdad
First pages:
19T1582 - p.pdf
Abstract:
Background : Chlamydia trachomatis is an obligate intracellular bacterium characterized by biphasic developmental cycle of replication. The organism is recognized as one of the causes of preterm labour in our country.Aim of the study : Detection of Chlamydia trachomatis as acause of preterm labour by using PCR versus ELISA, and effect of infection on fetal birth outcome.Design : Prospective Case - control study. Patient and methods : Endocervical swabs were collected from 80 pregnant women, with gestational age 28th—36th+ 6 weeks ,estimated from 1st day of last menstrual period and /or by 1st or 2nd trimester uttrasonographic assessment. These specimens were processed using molecular (polymerase chain reaction, PCR) and enzyme immunoassay (EIASA).Result : By using polymerase chain reaction (PCR) has a sensitivity of 80% to 95% and a specificity of 99% . in this study ,PCR test is better than ELISA test for the diagnosis the chlamydia infection ( P value 0.0001) in preterm labour.Conclusions : Nucleic acid amplification test by using Polymerase chain reaction proved to be superior and more efficient in the diagnosis of Chlamydia trachomatis than Enzyme linked immune sorbent assay ,and Chlamydia infection did not affect fetal birth outcome .
