دراسة مصلية وجزيئية لبكتريا Escherichia coli المعزولة من بعض الاغذية الحيوانية ومن حالات سريرية في محافظة بابل == Molecular and serological study of the bacteria Escherichia coli isolated from some animal foods and clinical cases in Babylon province
Author name:
محمد حسن عبد الكاظم الكعبي
Supervisor name:
الهام سعيد عبد الكريم
General topic:
Biology
Specific topic:
Microbiology - Bacteria
University:
University of Baghdad
Language:
Arabic
University location:
Baghdad
First pages:
24T3135 - p.pdf
Abstract:
This study isolation and Identification of Esherichia coli in Babylon province. Atotal of 206 samples were obtamel which Include 86 stool sample from patients with diarrhea for both sexy and different ages in AL - kasem and AL - Hashimia hospitals from 2 - 2 - 2015 to 30 - 3 - 2015.60 samples of ground beef meat and 60 samples of Localy raw cheeses were obtamil from Hlla , Kasem, Hashimia and Hamza states form the period from 4 - 2 - 2015 to 17 - 4 - 2015 .2. 145 Isolates characterrizd as E.coli depending on the characteristics of the colonies phenotypic and microscopic when grown in diffrerential selective media as well as biochemical test to diagnose Isolates .Then the Identification of these species were confirmal by using Api20E system . 77(89.53 %) E.coli Isolates were obtamel from stoolsamples ,33(22.75%) from ground beef meat , samples 35(24.14%) from Localy raw cheeses samples.3. Serological Identification for 145 E.coli Isolates showed that 31 Isolates (21.8%) were belonged to O157 : H7 ,also results showed 69 E.coli Isolates (47.58%)were belonged to polyvalent II (O111,O119,O26,O55,O126) ,22(15.17%) belonged to polyvalent III (O86,O114,O123,O127,O128),11(7.59%) Isolates belonged to polyvalent IV (O112,O124,O142), 2 Isolstes .belonged to O128 Monovalent serotype from stooland cheese samples and 2Isolates belonged to O159 Monovalent from stool samples .4. Results of Virulence factors of E.coli Isolates showed the ability of 66(45.51%)Producing hemolysin (α,β),E.coli Isolates showed containing tow types of adherence CFA/1 and CFA/III , and tow ratios were 104(71.72%) and 77(53.10%)Respectively ,109(75.7%) Isolates shwed ability to produce Biofilm and 122(84.15%) Isolates showed ability to produce β - Lactamase .5. The Antibiot snsceptibility to all E.coli O157 : H7 Isolate .were performed according to Kirby Bauer testing ,showed ahigh degree of resistance 100% to Ampicillin and Mmeropenem, All Isolates showed Susceptibility 0% to Impenem and Amikacin ,Norfloxcin 96.77% and Gentamycin 90.32%.while resistance to Ceftazidine, Pipracillin ,Ofloxacine and Trimethoprime 77.41%, 70.96 %70 .96%67 74% respectively .6. The molecular Investigation of the genes stx1,stx2 hlyA and flic : H7 was made by taking out the bacterial DNA of all E.coliO157 : H7.Isolates using polymerase chain reaction PCR techniques , results showed 26(83.87%)Isolates gene positive result for stx1 at (180 bp) ,9(29.03%) gave positive result for stx2 at(255bp),all Isolates 100% gave hlyA at (534bp),23(74.19%) gave positive result for flic : H7 at (625bp).
