دراسة تصنيفية وجزيئية لثمانية انواع من العقارب Scorpions Arachnida : Scorpiones في بعض مناطق العراق == Taxonomic and Molecular Study for Eight Species of the Scorpions (Arachnida : Scorpiones) in Some Areas of Iraq
Author name:
زينة نبيل نصيف العزاوي
Supervisor name:
صباح فرج عبد الاحد
General topic:
Biology
Specific topic:
Zoology - Invertebrates
Degree:
Doctorate
University:
University of Baghdad
Language:
Arabic
University location:
Baghdad
First pages:
24T3467 - p.pdf
Abstract:
The study was including two sides : taxonomic description and genetic, for One hundred of scorpions were collected from different parts from central and southern provinces of Iraq (Baghdad, Diyala, Salahuddin, Karbala, Wasit, Najaf, Qadisiyah, Dhi Qar and Basra). The taxonomic side was included using eighty six samples to study the external characteristics in addition to make some specific measurements to length of the samples, while the results of the taxonomic study showed large variation in the external characteristics as well as present of differences in the measurements which were taken among members of the same species. The results showed obtaining from eight species five recorded previously in Iraq and described for first time and three species recorded and described for the first time in Iraq, as well as reordered new species discovered at first time in world . The study has been including set diagnostic key to isolation the species in addition to determinate diagnostic characterization for each type supported by illustrative photography as follows : 1 - Family : Buthidae ( 74 sample) a) - Androctonus crassicauda ,Olivier,1807,(49 sample)b) - Compsobuthus iraqensis ,sp.nov,Vachon,1949,(1 sample)c) - Hottentotta zagrosensis , Kovarik,1997(3 sample)d) - Hottentotta judaicus ,Simon,1872,(1 sample)e) - Mesobuthus eupeus ,Koch,1839,(17 sample)f) - Othochirus scrobiculosus ,Grube,1873,(3 sample) ,2 - Family Bothriuridae( 2 sample)a ) - Bothriurus nendai ,Affilastro and Mauro,2010, (2 sample)3 - Family Scorpionidae( 10 sample)a) - Scorpio maurus ,Linnaeus,1758(10 sample)* Genus and species were recorded previously in Iraq, and they were described for the first time in Iraq. Species was new recorded in Iraq, and it was described for the first time in Iraq.*** Species was new recorded in Iraq and the world, and it was described for the first time in Iraq and the world.The genetic study was included used fifteen samples of scorpions which collected from different regions at the provinces of central and southern Iraq (Baghdad, Diyala, Dhi Qar, Najaf, Qadisiyah, Basra). On this side was utilized a technology polymerase chain reaction (PCR) for the purpose of finding genetic diversity between the species and for the detection of the relevant document types with each other on gene 1 Cytochrome Oxidase (CO1) which present on the piece of DNA mitochondria (mtDNA) and extract from the pair III and IV of the legs and by present of a pair of specialized primers (ZCO1, NCO1). The results are displayed on one band for eight samples out of fifteen sample and the same level and the molecular weight of 1057 base pairs of the primer (ZCO1), but appearance just two bands for two samples of scorpions Androctonus crassicauda and Scorpio maurus which collected from provinces Baghdad and Najaf at the same level and molecular weight 793 base pairs of the primer (NCO1).The sequence sequential of nitrogen bases was conducted for two samples which they scorpions Androctonus crassicauda and Scorpio maurus when used primers ZCO1 and NCO1 after amplification of the DNA piece by using PCR reaction device that contains a gene CO1 located in mitochondria. The results of primer ZCO1 and sequence nucleotides in Iraqi samples indicated present of many variations in the gene under study compared with standard samples and registered on Gen Bank site (NCBI) where found a case of polymorphism resulting from mutations replacement of Substitution genetic for the sequences nucleotides of the primer and by up to 23.9% and 24.3% respectively as the highest proportion especially at the base of nitrogenous T→C and the lowest rate of 2.8% and 5.4% of the substitution nitrogenous base A→T and nitrogenous base G→A respectively.When using primer NCO1 and their sequence nucleotides to scorpion sample Androctonus crassicauda that belong to Buthidae family noticed lack of match in the gene with samples recorded at NCBI site. While no similarity found in scorpion sample Scorpio maurus that belong to Scorpionidae family when used anterior direction for primer NFCO1 with recorded samples in NCBI site. While used posterior direction for primer NRCO1 for same sample was observed numbers of mutations compared to the recorded sample on NCBI site including substitution genetic mutations of sequences nucleotide gene and especially at the base of nitrogenous C→T and T→C at percentage 22.9% as the highest percentage. The nitrogenous base A→T recorded lower percentage in 2.0% and the twonitrogenous bases C and A in 2.0%, which represents deletion mutation in the nucleotide sequence of Iraqi sample. This differences give a significant advantage to the Iraqi samples that represented two species Androctonus crassicauda and Scorpio maurus compared with registered samples in the world. In addition to the presence of genetic diversity in recorded Iraqi samples.
